LOCUS       pHBBAD168               4918 bp    DNA     circular UNA 05-NOV-2018
DEFINITION  2 reads from HBPCR168 extraction assembled using Geneious.
ACCESSION   urn.local...4t-9pmm5iu
VERSION     urn.local...4t-9pmm5iu
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     gene            complement(465..1343)
                     /note="Geneious type: Gene"
                     /standard_name="AraC"
     misc_feature    1373..1388
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="araO2 reg"
     misc_feature    1530..1541
                     /note="Geneious type: Regulatory Sequence"
                     /standard_name="araO1 reg"
     gene            1572..1585
                     /note="Geneious type: Gene"
                     /standard_name="CAP_BS"
     gene            1582..1620
                     /note="Geneious type: Gene"
                     /standard_name="AraI1I2"
     promoter        1617..1645
                     /note="Geneious type: Promoter"
                     /standard_name="ARA prom"
     gene            1686..1702
                     /note="Geneious type: Gene"
                     /standard_name="T7_transl_en_RBS"
     CDS             1711..1836
                     /standard_name="NpuDnaBmin2_C39_K4posOrtho-GB1"
     misc_feature    complement(1727..1749)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="L287"
     misc_feature    1745
                     /note="Geneious type: mismatch"
                     /standard_name="A/T"
     misc_feature    1746
                     /note="Geneious type: mismatch"
                     /standard_name="A/C"
     primer_bind     1750..1772
                     /standard_name="L286"
     misc_feature    1759
                     /note="Geneious type: mismatch"
                     /standard_name="G/A"
     CDS             1837..2031
                     /standard_name="GB1-H6"
     misc_feature    2011..2028
                     /note="Geneious type: Tag"
                     /standard_name="6xHis tag"
     terminator      2118..2274
                     /note="Geneious type: Terminator"
                     /standard_name="rrnB term"
     terminator      2240..2283
                     /note="Geneious type: Terminator"
                     /standard_name="rrnB_T1 term"
     terminator      2415..2442
                     /note="Geneious type: Terminator"
                     /standard_name="rrnB_T2 term"
     promoter        2482..2510
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
     misc_feature    2552..3412
                     /note="Geneious type: Marker"
                     /standard_name="amp marker"
     misc_feature    3471..3777
                     /note="Geneious type: Origin of Replication"
                     /standard_name="f1 origin"
     misc_feature    4004..4623
                     /note="Geneious type: Origin of Replication"
                     /standard_name="pBR322 origin"
ORIGIN      
        1 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
       61 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
      121 gaggttttca ccgtcatcac cgaaacgcgc gaggcagcaa ggagatggcg cccaacagtc
      181 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
      241 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
      301 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctaattc tcatgtttga
      361 cagcttatca tcgatgcata atgtgcctgt caaatggacg aagcagggat tctgcaaacc
      421 ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg
      481 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg
      541 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg
      601 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc
      661 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac
      721 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga
      781 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg
      841 ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc
      901 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc
      961 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta
     1021 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga
     1081 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc
     1141 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga
     1201 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg
     1261 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg
     1321 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat
     1381 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc
     1441 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca
     1501 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac
     1561 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga
     1621 cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttgggc tagaaataat
     1681 tttgtttaac tttaagaagg agatatacat atgagtgata tttattggga tgaaatagtt
     1741 tcaaaggaat atagtggtaa ggaagaagtg tttgacctca ccgttcctgg tttgcataat
     1801 tttgttgcaa ataatattat tgttcacaat tccattggta cccagtacaa acttatcctg
     1861 aacggtaaaa ccctgaaagg tgaaaccacc accgaagctg ttgacgctgc taccgcggaa
     1921 aaagttttca aacagtacgc taacgacaac ggtgttgacg gtgaatggac ctacgacgac
     1981 gctaccaaaa cctacaccgt aacggaattc catcatcatc atcatcatta agcttggctg
     2041 ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
     2101 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
     2161 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtccccca tgcgagagta
     2221 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtgc aaagactggg cctttcgttt
     2281 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     2341 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     2401 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
     2461 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2521 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     2581 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     2641 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     2701 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     2761 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     2821 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     2881 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     2941 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3001 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3061 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     3121 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3181 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3241 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3301 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     3361 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     3421 ccaagtttac tcatatatac tttagattga tttacgcgcc ctgtagcggc gcattaagcg
     3481 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3541 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3601 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3661 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3721 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3781 tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3841 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     3901 ttacaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3961 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4021 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4081 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4141 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     4201 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4261 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4321 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4381 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     4441 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4501 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4561 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4621 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4681 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4741 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     4801 cggtattttc tccttacgca tctgtgcggt atttcacacc ggatatggtg cactctcagt
     4861 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtga
//
