LOCUS       pLH-sgRNA-Sirius-8XMS2        8557 bp    DNA     circular SYN 23-AUG-2018
DEFINITION  -.
ACCESSION   -
KEYWORDS    -.
SOURCE      -.
  ORGANISM  
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            http://www.invitrogen.com/
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FEATURES             Location/Qualifiers
     misc_feature    join(5627..>5643,complement(<6699..>6699),complement(<5644..>5644),<6700..>6795)
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="sgRNA cassette BfuAI sites [Split]"
     misc_feature    597..1622
                     /vntifkey="21"
                     /label=Hygromycin
     misc_feature    3139..3999
                     /vntifkey="21"
                     /label=Amp
     promoter        7059..7308
                     /vntifkey="29"
                     /label=U6\promoter
     misc_recomb     6310..6514
                     /vntifkey="86"
                     /label=REV
     misc_recomb     5792..6119
                     /vntifkey="86"
                     /label=5'\Gag
     misc_recomb     5701..5809
                     /vntifkey="86"
                     /label=Psi
     misc_marker     1744..1988
                     /vntifkey="22"
                     /label=SIN/3'\LTR
     misc_marker     5454..5634
                     /vntifkey="22"
                     /label=HIV\5'\LTR
     rep_origin      4200..4740
                     /vntifkey="33"
                     /label=pUC\Ori
     promoter        3040..3138
                     /vntifkey="30"
                     /label=Bla\Promoter
     polyA_signal    2050..2180
                     /vntifkey="25"
                     /label=SV40\PolyA
     misc_feature    1723..1736
                     /vntifkey="21"
                     /label=PPT
     misc_feature    1744..1797
                     /vntifkey="21"
                     /label=Partial\U3
     misc_feature    7648..7953
                     /vntifkey="21"
                     /label=CcdB
     misc_feature    7974..8339
                     /vntifkey="21"
                     /label=sgRNA-Sirius-8XMS2
BASE COUNT     2228 a      2033 c      2274 g      2022 t 
ORIGIN
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 
      241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 
      481 gaccgaatca ccgacctctc tccccagggg gatccaccgg atcaagcttg ccagctgggg 
      541 cgccctctgg taaggttggg aagccctgca gatctgatcc cggggggcaa tgagatatga 
      601 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg 
      661 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag 
      721 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt 
      781 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg 
      841 aatttagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag 
      901 atctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga 
      961 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg 
     1021 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact 
     1081 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga 
     1141 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca 
     1201 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt 
     1261 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta 
     1321 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc 
     1381 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca 
     1441 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg 
     1501 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg 
     1561 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat 
     1621 agagtagatg ccgaccggga tctatcgata ccgtcgacct cgaggggggg cccggtacct 
     1681 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 
     1741 ggactggaag ggctaattca ctcccaacga agaaaagatc tgctttttgc ttgtactggg 
     1801 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 
     1861 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 
     1921 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 
     1981 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 
     2041 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     2101 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     2161 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc 
     2221 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 
     2281 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 
     2341 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc 
     2401 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 
     2461 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 
     2521 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc 
     2581 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 
     2641 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 
     2701 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 
     2761 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 
     2821 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 
     2881 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 
     2941 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct 
     3001 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 
     3061 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 
     3121 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 
     3181 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 
     3241 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 
     3301 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 
     3361 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 
     3421 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 
     3481 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 
     3541 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 
     3601 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 
     3661 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 
     3721 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 
     3781 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 
     3841 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 
     3901 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 
     3961 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 
     4021 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 
     4081 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 
     4141 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 
     4201 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 
     4261 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 
     4321 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 
     4381 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 
     4441 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 
     4501 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 
     4561 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 
     4621 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 
     4681 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 
     4741 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 
     4801 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 
     4861 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 
     4921 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 
     4981 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 
     5041 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 
     5101 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 
     5161 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa 
     5221 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc 
     5281 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt 
     5341 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg 
     5401 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc 
     5461 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
     5521 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
     5581 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
     5641 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg 
     5701 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat 
     5761 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg 
     5821 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa 
     5881 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct 
     5941 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac 
     6001 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca 
     6061 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa 
     6121 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga 
     6181 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag 
     6241 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag 
     6301 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga 
     6361 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc 
     6421 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc 
     6481 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt 
     6541 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta 
     6601 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta 
     6661 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga 
     6721 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa 
     6781 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa 
     6841 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat 
     6901 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag 
     6961 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc 
     7021 gatcacgaga ctagcctcga gcggccgccc ccttcaccga gggcctattt cccatgattc 
     7081 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg 
     7141 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt 
     7201 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt 
     7261 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccggt gtcttcgagg 
     7321 cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat 
     7381 atatactgat atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt 
     7441 acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct 
     7501 ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga 
     7561 aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt 
     7621 ttgctgacga gaacagggac tggtgaaatg cagtttaagg tttacaccta taaaagagag 
     7681 agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg 
     7741 atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac 
     7801 ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg 
     7861 ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa 
     7921 aacgccatta acctgatgtt ctggggaata taaaccctgc aggacgaaga cccgtttgag 
     7981 agctactgcc atgaggaatg accaccaggc attccgatcc gacgatggac catcaggcca 
     8041 tcgagctgca gaagtgacga ccacgcactt cggagtgaca gaggaggatc acccctctgg 
     8101 ccaccagagt agagcatcag cctactccgg acaactacgg aggaccaccc cgtaggagta 
     8161 gagcgaggag caccagccct cgcgtgtgac gatgacgatc acgcatcgtc acacaaacta 
     8221 ctcaaatgtc cgaaaggtgg caaacactcc aaagcagcta aacggatcaa acatggcagt 
     8281 agcaagttca aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct 
     8341 ttttttgaat tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa 
     8401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata 
     8461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg 
     8521 gacagcagag atccactttg gccgcggctc gaggggg 
//
