LOCUS       pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7       10269 bp    DNA     circular SYN 23-AUG-2018
DEFINITION  -.
ACCESSION   -
KEYWORDS    -.
SOURCE      -.
  ORGANISM  
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            http://www.invitrogen.com/
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FEATURES             Location/Qualifiers
     misc_feature    join(5627..>5643,complement(<6699..>6699),complement(<5644..>5644),<6700..>6795)
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="sgRNA cassette BfuAI sites [Split]"
     misc_feature    3421..4281
                     /vntifkey="21"
                     /label=Amp
     misc_recomb     6592..6796
                     /vntifkey="86"
                     /label=REV
     misc_recomb     6074..6401
                     /vntifkey="86"
                     /label=5'\Gag
     misc_recomb     5983..6091
                     /vntifkey="86"
                     /label=Psi
     misc_marker     2026..2270
                     /vntifkey="22"
                     /label=SIN/3'\LTR
     misc_marker     5736..5916
                     /vntifkey="22"
                     /label=HIV\5'\LTR
     rep_origin      4482..5022
                     /vntifkey="33"
                     /label=pUC\Ori
     promoter        3322..3420
                     /vntifkey="30"
                     /label=Bla\Promoter
     polyA_signal    2332..2462
                     /vntifkey="25"
                     /label=SV40\PolyA
     misc_feature    2005..2018
                     /vntifkey="21"
                     /label=PPT
     misc_feature    2026..2079
                     /vntifkey="21"
                     /label=Partial\U3
     misc_feature    7936..8241
                     /vntifkey="21"
                     /label=CcdB
     misc_feature    2051..2056
                     /vntifkey="21"
                     /label=(BbsI)
     misc_feature    1..507
                     /vntifkey="21"
                     /label=hPGK\promoter
     misc_feature    1128..1184
                     /vntifkey="21"
                     /label=P2A
     misc_feature    1200..1910
                     /vntifkey="21"
                     /label=TagBFP2
     misc_feature    1500..1505
                     /vntifkey="21"
                     /label=(BbsI)
     misc_feature    1785..1787
                     /vntifkey="21"
                     /label=V-to-I\mutation
     misc_feature    7331..7586
                     /vntifkey="21"
                     /label=human\U6\promoter
     misc_feature    1911..1931
                     /vntifkey="21"
                     /label=NLS
     misc_feature    525..1118
                     /vntifkey="21"
                     /label=Puromycin
     misc_feature    6425..6432
                     /vntifkey="21"
                     /label=(Not\I)
     misc_feature    8654..8965
                     /vntifkey="21"
                     /label=mouse\U6\promoter
     misc_feature    9639..10038
                     /vntifkey="21"
                     /label=sgRNA-Sirius-8XPP7
     misc_feature    9315..9620
                     /vntifkey="21"
                     /label=CcdB
     misc_feature    8262..8627
                     /vntifkey="21"
                     /label=sgRNA-Sirius-8XMS2
BASE COUNT     2714 a      2503 c      2723 g      2329 t 
ORIGIN
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 
      241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 
      481 gaccgaatca ccgacctctc tccccagggg gatccgccac catgaccgag tacaagccca 
      541 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt 
      601 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca 
      661 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg 
      721 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg 
      781 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc 
      841 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca 
      901 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg 
      961 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca 
     1021 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag 
     1081 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgc tagcggtgct actaacttca 
     1141 gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctggtagt ggaacgcgta 
     1201 tggtgtctaa gggcgaagag ctgattaagg agaacatgca catgaagctg tacatggagg 
     1261 gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg 
     1321 gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca 
     1381 tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc cagggcatcc 
     1441 ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg 
     1501 aagatggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct 
     1561 acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg cagaagaaaa 
     1621 cactcggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggc ctggaaggca 
     1681 gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac gccaagacca 
     1741 catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcatctac tatgtggact 
     1801 acagactgga aagaatcaag gaggccaaca acgaaaccta cgtcgagcag cacgaggtgg 
     1861 cagtggccag atactgcgac ctccctagca aactggggca caagcttaat ccaaagaaaa 
     1921 agcggaaagt gtgagtcgac ctcgaggggg ggcccggtac ctttaagacc aatgacttac 
     1981 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt 
     2041 cactcccaac gaagaaaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag 
     2101 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 
     2161 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 
     2221 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt 
     2281 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt 
     2341 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 
     2401 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 
     2461 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 
     2521 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 
     2581 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc 
     2641 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg 
     2701 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc 
     2761 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 
     2821 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 
     2881 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 
     2941 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 
     3001 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 
     3061 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 
     3121 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 
     3181 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 
     3241 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact 
     3301 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 
     3361 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 
     3421 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 
     3481 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 
     3541 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 
     3601 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 
     3661 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 
     3721 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 
     3781 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 
     3841 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 
     3901 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 
     3961 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 
     4021 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 
     4081 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 
     4141 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 
     4201 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 
     4261 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 
     4321 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 
     4381 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 
     4441 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 
     4501 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 
     4561 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 
     4621 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 
     4681 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 
     4741 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 
     4801 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 
     4861 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 
     4921 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 
     4981 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 
     5041 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 
     5101 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 
     5161 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 
     5221 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 
     5281 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag 
     5341 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga 
     5401 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc 
     5461 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg 
     5521 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag 
     5581 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag 
     5641 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag 
     5701 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct 
     5761 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 
     5821 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 
     5881 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa 
     5941 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac 
     6001 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta 
     6061 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg 
     6121 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg 
     6181 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg 
     6241 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag 
     6301 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga 
     6361 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca 
     6421 gcaagcggcc actgatcttc agacctggag gaggagatat gagggacaat tggagaagtg 
     6481 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa 
     6541 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt 
     6601 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca 
     6661 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc 
     6721 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg 
     6781 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac 
     6841 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga 
     6901 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa 
     6961 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg 
     7021 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata 
     7081 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac 
     7141 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc 
     7201 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca 
     7261 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc 
     7321 gagcggccgc ccccttcacc gagggcctat ttcccatgat tccttcatat ttgcatatac 
     7381 gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag 
     7441 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat 
     7501 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt 
     7561 tatatatctt gtggaaagga cgaaacaccg gtgtcttcga atgcatggct tactaaaagc 
     7621 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat 
     7681 gtatacccga agtatgtcaa aaagaggtgt gctatgaagc agcgtattac agtgacagtt 
     7741 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa 
     7801 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc 
     7861 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga 
     7921 acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt 
     7981 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc 
     8041 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat 
     8101 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt 
     8161 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac 
     8221 ctgatgttct ggggaatata aaccctgcag gacgaagacc cgtttgagag ctactgccat 
     8281 gaggaatgac caccaggcat tccgatccga cgatggacca tcaggccatc gagctgcaga 
     8341 agtgacgacc acgcacttcg gagtgacaga ggaggatcac ccctctggcc accagagtag 
     8401 agcatcagcc tactccggac aactacggag gaccaccccg taggagtaga gcgaggagca 
     8461 ccagccctcg cgtgtgacga tgacgatcac gcatcgtcac acaaactact caaatgtccg 
     8521 aaaggtggca aacactccaa agcagctaaa cggatcaaac atggcagtag caagttcaaa 
     8581 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttttctaga 
     8641 ggcaaggatc gatgatccga cgccgccatc tctaggcccg cgccggcccc ctcgcacaga 
     8701 cttgtgggag aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag 
     8761 taactataga ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct 
     8821 acattttaca tgataggctt ggatttctat aagagataca aatactaaat tattatttta 
     8881 aaaaacagca caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat 
     8941 aaatatccct tggagaaaag ccttgtttgg tgtcttcgaa tgcatggctt actaaaagcc 
     9001 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg 
     9061 tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca gtgacagttg 
     9121 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag 
     9181 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca 
     9241 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa 
     9301 cagggactgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc 
     9361 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc 
     9421 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata 
     9481 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta 
     9541 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc 
     9601 tgatgttctg gggaatataa accctgcagg acgaagacgt ttgagagcta ctgccatgag 
     9661 gagcagacga tatggcgtcg ctccgatccg accagcagag catatgggct cgctggggct 
     9721 gcagcagcag aggatatggc ctcgctgcgg agtgacgagc agaccatatg gggtcgctcg 
     9781 gccaccagaa gcagaagata tggcttcgct tccggacaac tagcagatca tatgggatcg 
     9841 ctaggagtag agtagcagat gatatggcat cgctacgtgt gacaagcaga acatatgggt 
     9901 tcgcttgtca cacaaactac tcaaatgtcc gaaaggtggc aaacactcca aagcagccaa 
     9961 acggatcaaa catggcagta gcaagttcaa ataaggctag tccgttatca acttgaaaaa 
    10021 gtggcaccga gtcggtgctt tttttatcga tggcaaggga attctcgacc tcgagacaaa 
    10081 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg 
    10141 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta 
    10201 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggccgcggc 
    10261 tcgaggggg 
//
