LOCUS       pTet-IUP                7794 bp ds-DNA     circular     19-MAR-2018
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     misc_feature    2668..2687
                     /label="High GC Redesign"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     protein_bind    2373..2391
                     /label="tetR binding site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      6003..6309
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             5228..5776
                     /label="idi"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3753..5211
                     /label="gBlock 2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(1695..2321)
                     /label="ORF frame 2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      5986..6441
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(6537..7349)
                     /label="KanR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5212..5227
                     /label="Calced RBS"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4179..4212
                     /label="RBS 796k"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2430..3783
                     /label="gBlock 1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             5818..5835
                     /label="6x His Tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4213..5211
                     /label="atIPK"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2430..4178
                     /label="Choline Kinase"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2430..5211
                     /label="From pET-ProD-GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      5903..5949
                     /label="T7\terminator"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      join(7395..7794,1..283)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
       61 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      121 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      181 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      241 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      301 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      361 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
      421 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
      481 gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca
      541 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
      601 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
      661 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
      721 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt
      781 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc
      841 tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt
      901 aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga
      961 tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg
     1021 cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta
     1081 atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca
     1141 taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga
     1201 ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct
     1261 cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc
     1321 tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct
     1381 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
     1441 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
     1501 cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga
     1561 cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt
     1621 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat
     1681 taattgcgtt gcgcttaaga cccactttca catttaagtt gtttttctaa tccgcatatg
     1741 atcaattcaa ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa taattcgata
     1801 gcttgtcgta ataatggcgg catactatca gtagtaggtg tttccctttc ttctttagcg
     1861 acttgatgct cttgatcttc caatacgcaa cctaaagtaa aatgccccac agcgctgagt
     1921 gcatataatg cattctctag tgaaaaacct tgttggcata aaaaggctaa ttgattttcg
     1981 agagtttcat actgtttttc tgtaggccgt gtacctaaat gtacttttgc tccatcgcga
     2041 tgacttagta aagcacatct aaaactttta gcgttattac gtaaaaaatc ttgccagctt
     2101 tccccttcta aagggcaaaa gtgagtatgg tgcctatcta acatctcaat ggctaaggcg
     2161 tcgagcaaag cccgcttatt ttttacatgc caatacaatg taggctgctc tacacctagc
     2221 ttctgggcga gtttacgggt tgttaaacct tcgattccga cctcattaag cagctctaat
     2281 gcgctgttaa tcactttact tttatctaat ctagacatca ttaattccta atttttgttg
     2341 acactctatc gttgatagag ttattttacc actccctatc agtgatagag aaaagaattc
     2401 aaaagatctt ttaagaagga gatatacatA TGGTGCAGGA GTCCCGCCCC GGCTCGGTCC
     2461 GGTCGTATTC CGTGGGCTAC CAGGCCCGGT CGCGGTCGTC GTCCCAGCGC CGCCATTCGC
     2521 TCACGCGGCA GCGCAGCAGC CAGCGGCTCA TCCGGACGAT CTCCATCGAG AGCGATGTGA
     2581 GCAATATCAC GGACGATGAT GATCTGCGGG CGGTGAATGA AGGGGTGGCC GGGGTCCAGC
     2641 TCGACGTCTC CGAGACGGCG AACAAAGGGC CaCGCCGGGC CAGtGCCACC GATGTCACCG
     2701 ACTCGCTGGG CTCCACGTCC AGCGAATATA TCGAGATCCC CTTCGTGAAA GAGACGCTGG
     2761 ACGCGAGCCT CCCCTCGGAT TACCTCAAAC AAGACATCCT GAACCTGATC CAATCCCTGA
     2821 AGATCTCGAA ATGGTACAAT AACAAAAAGA TCCAGCCCGT CGCCCAGGAC ATGAACCTCG
     2881 TCAAAATCTC CGGCGCGATG ACCAATGCGA TCTTCAAGGT GGAGTACCCG AAACTGCCGT
     2941 CCCTCCTGCT GCGGATCTAT GGCCCGAATA TCGATAACAT CATCGACCGC GAATATGAAC
     3001 TCCAGATCCT CGCGCGGCTC TCGCTGAAAA ACATCGGGCC GTCCCTGTAC GGCTGCTTCG
     3061 TGAATGGGCG CTTCGAGCAG TTCCTCGAAA ACTCCAAAAC GCTGACCAAG GATGATATCC
     3121 GGAACTGGAA AAACTCGCAA CGGATCGCCC GCCGCATGAA GGAGCTGCAT GTGGGCGTGC
     3181 CCCTCCTCTC GTCGGAGCGG AAGAATGGGA GCGCCTGCTG GCAAAAAATC AACCAATGGC
     3241 TCCGCACGAT CGAGAAGGTG GATCAGTGGG TCGGGGACCC GAAGAACATC GAGAACAGCC
     3301 TCCTCTGCGA AAATTGGTCC AAATTCATGG ACATCGTCGA TCGGTACCAC AAGTGGCTGA
     3361 TCAGCCAAGA ACAAGGGATC GAGCAAGTCA ACAAAAATCT GATCTTCTGC CATAATGATG
     3421 CCCAATACGG GAATCTCCTC TTCACCGCGC CCGTCATGAA CACCCCCTCC CTGTATACCG
     3481 CGCCGAGCTC GACCTCCCTG ACGTCCCAAA GCAGCAGCCT CTTCCCCTCG TCCAGCAACG
     3541 TGATCGTCGA TGATATCATC AATCCCCCGA AGCAAGAACA ATCCCAAGAT TCCAAACTCG
     3601 TGGTCATCGA TTTCGAATAC GCCGGGGCCA ATCCCGCCGC GTACGATCTC GCCAATCACC
     3661 TCTCGGAATG GATGTACGAC TATAATAACG CCAAAGCCCC GCACCAGTGC CACGCCGACC
     3721 GGTACCCCGA CAAGGAGCAA GTGCTCAACT TCCTGTATTC GTATGTCAGC CATCTCCGCG
     3781 GCGGGGCCAA AGAGCCCATC GATGAAGAAG TCCAGCGCCT CTATAAATCG ATCATCCAGT
     3841 GGCGCCCCAC GGTGCAGCTC TTCTGGTCGC TGTGGGCGAT CCTGCAAAGC GGCAAGCTGG
     3901 AAAAAAAAGA AGCCAGCACC GCCATCACCC GCGAAGAAAT CGGGCCCAAT GGGAAAAAGT
     3961 ATATCATCAA GACGGAGCCC GAGTCGCCCG AAGAGGACTT CGTCGAAAAT GACGACGAAC
     4021 CCGAAGCCGG CGTGTCGATC GATACCTTCG ACTACATGGC CTACGGGCGG GACAAGATCG
     4081 CGGTGTTCTG GGGGGACCTG ATCGGGCTGG GCATCATCAC GGAGGAGGAA TGCAAGAACT
     4141 TCTCGAGCTT CAAATTCCTC GACACCAGCT ACCTGTAAGT ACAAGAAAAG TCAGTAGTCT
     4201 AAGGAGGTAA GCATGGAACT CAATATCAGC GAAAGCCGGT CGCGCAGCAT CCGGTGCATC
     4261 GTGAAGCTCG GGGGCGCGGC CATCACCTGC AAAAACGAAC TCGAAAAGAT CCATGACGAA
     4321 AACCTCGAAG TGGTGGCCTG CCAACTGCGG CAAGCGATGC TGGAAGGCTC CGCCCCCTCC
     4381 AAAGTCATCG GCATGGACTG GTCCAAACGG CCGGGCTCCT CCGAAATCTC CTGCGATGTG
     4441 GACGACATCG GCGACCAGAA ATCGAGCGAA TTCTCGAAGT TCGTGGTCGT CCACGGGGCG
     4501 GGCTCGTTCG GCCATTTCCA AGCGTCGCGG TCGGGCGTCC ATAAAGGGGG CCTGGAGAAG
     4561 CCCATCGTCA AAGCCGGGTT CGTCGCGACC CGGATCTCGG TCACCAATCT CAATCTCGAG
     4621 ATCGTCCGCG CCCTGGCCCG GGAAGGCATC CCGACGATCG GGATGAGCCC CTTCAGCTGC
     4681 GGCTGGTCCA CCTCCAAACG GGATGTCGCG TCGGCGGATC TCGCGACGGT CGCCAAGACC
     4741 ATCGACTCGG GCTTCGTGCC CGTGCTCCAT GGGGACGCGG TCCTGGACAA CATCCTGGGC
     4801 TGCACGATCC TGTCCGGCGA TGTCATCATC CGGCACCTGG CCGACCATCT CAAGCCGGAA
     4861 TACGTCGTGT TCCTGACCGA CGTGCTCGGG GTCTATGACC GGCCCCCGAG CCCGTCGGAG
     4921 CCGGACGCCG TCCTGCTCAA GGAGATCGCC GTCGGGGAGG ATGGCTCCTG GAAGGTGGTC
     4981 AACCCCCTCC TGGAACATAC CGACAAAAAG GTGGATTATT CCGTCGCGGC CCACGATACC
     5041 ACGGGGGGGA TGGAAACCAA AATCTCGGAA GCCGCCATGA TCGCCAAGCT CGGCGTGGAT
     5101 GTGTACATCG TGAAAGCCGC GACCACCCAT TCGCAGCGGG CGCTCAATGG CGACCTGCGC
     5161 GACTCCGTCC CCGAAGACTG GCTGGGGACC ATCATCCGCT TCAGCAAATA AAACTAAGGA
     5221 GGTCTATatg CAAACGGAAC ACGTCATTTT ATTGAATGCA CAGGGAGTTC CCACGGGTAC
     5281 GCTGGAAAAG TATGCCGCAC ACACGGCAGA CACCCGCTTA CATCTCGCGT TCTCCAGTTG
     5341 GCTGTTTAAT GCCAAAGGAC AATTATTAGT TACCCGCCGC GCACTGAGCA AAAAAGCATG
     5401 GCCTGGCGTG TGGACTAACT CGGTTTGTGG GCACCCACAA CTGGGAGAAA GCAACGAAGA
     5461 CGCAGTGATC CGCCGTTGCC GTTATGAGCT TGGCGTGGAA ATTACGCCTC CTGAATCTAT
     5521 CTATCCTGAC TTTCGCTACC GCGCCACCGA TCCGAGTGGC ATTGTGGAAA ATGAAGTGTG
     5581 TCCGGTATTT GCCGCACGCA CCACTAGTGC GTTACAGATC AATGATGATG AAGTGATGGA
     5641 TTATCAATGG TGTGATTTAG CAGATGTATT ACACGGTATT GATGCCACGC CGTGGGCGTT
     5701 CAGTCCGTGG ATGGTGATGC AGGCGACAAA TCGCGAAGCC AGAAAACGAT TATCTGCATT
     5761 TACCCAGCTT AAAtaacgaa ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac
     5821 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
     5881 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     5941 ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg
     6001 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6061 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6121 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6181 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     6241 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6301 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6361 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     6421 aaatattaac gtttacaatt tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6481 tttgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa
     6541 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     6601 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     6661 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     6721 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     6781 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
     6841 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     6901 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     6961 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     7021 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     7081 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     7141 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     7201 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     7261 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag
     7321 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     7381 gacagtttta ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7441 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7501 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7561 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     7621 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7681 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7741 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggg
//