LOCUS       pGNW-SmR                3982 bp    DNA     circular UNA 09-JAN-2019
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...1v-9u7h3df
VERSION     urn.local...1v-9u7h3df
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     regulatory      complement(61..163)
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="T0"
     gene            complement(173..889)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="ATGCGTAAAGGTGAAGAACTGTTCACCGGTGTTGTTCCGATCCTGGTTGA
                     ACTGGATGGTGATGTTAACGGCCACAAATTCTCTGTTCGTGGTGAAGGTGAAGGTGAT
                     GCAACCAACGGTAAACTGACCCTGAAATTCATCTGCACTACCGGTAAACTGCCGGTTC
                     CATGGCCGACTCTGGTGACTACCCTGACCTATGGTGTTCAGTGTTTTTCTCGTTACCC
                     GGATCACATGAAGCAGCATGATTTCTTCAAATCTGCAATGCCGGAAGGTTATGTACAG
                     GAGCGCACCATTTCTTTCAAAGACGATGGCACCTACAAAACCCGTGCAGAGGTTAAAT
                     TTGAAGGTGATACTCTGGTGAACCGTATTGAACTGAAAGGCATTGATTTCAAAGAGGA
                     CGGCAACATCCTGGGCCACAAACTGGAATATAACTTCAACTCCCATAACGTTTACATC
                     ACCGCAGACAAACAGAAGAACGGTATCAAAGCTAACTTCAAAATTCGCCATAACGTTG
                     AAGACGGTAGCGTACAGCTGGCGGACCACTACCAGCAGAACACTCCGATCGGTGATGG
                     TCCGGTTCTGCTGCCGGATAACCACTACCTGTCCACCCAGTCTAAACTGTCCAAAGAC
                     CCGAACGAAAAGCGCGACCACATGGTGCTGCTGGAGTTCGTTACTGCCGCAGGTATCA
                     CGCACGGCATGGATGAACTCTACAAATAA"
                     /annotation_group="msfGFP: 1,304 -> 2,020"
                     /label="msfGFP"
     regulatory      complement(890..903)
                     /created_by="User"
                     /label="RBS"
     regulatory      complement(925..965)
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="promoter 14g"
     CDS             1057..1962
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="aadA CDS"
     gene            1057..1962
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="aadA gene"
     misc_feature    complement(1985..2074)
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="lac upstream"
     misc_feature    complement(2067..2087)
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="O3"
     misc_feature    complement(2075..2112)
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="CAP site"
     -35_signal      complement(2123..2128)
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="-35"
     -10_signal      complement(2147..2152)
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="-10"
     misc_feature    complement(2159..2178)
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="O1"
     RBS             complement(2186..2189)
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="RBS"
     gene            2197..2508
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /modified_by="User"
                     /label="LacZα"
     gene            complement(2761..3132)
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /label="TraJ"
     misc_feature    complement(3212..3310)
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="Relaxation region (TraJ recognition sequence)"
     misc_feature    complement(3434..3825)
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="R6K"
ORIGIN      
        1 aggtagctgt aacatcagag attttgagac acaaatttaa atcgtaatta ttggggaccc
       61 ctggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
      121 ggagttctga ggtcattact ggatctatca acaggagtcc aagactagtc atttatttgt
      181 agagttcatc catgccgtgc gtgatacctg cggcagtaac gaactccagc agcaccatgt
      241 ggtcgcgctt ttcgttcggg tctttggaca gtttagactg ggtggacagg tagtggttat
      301 ccggcagcag aaccggacca tcaccgatcg gagtgttctg ctggtagtgg tccgccagct
      361 gtacgctacc gtcttcaacg ttatggcgaa ttttgaagtt agctttgata ccgttcttct
      421 gtttgtctgc ggtgatgtaa acgttatggg agttgaagtt atattccagt ttgtggccca
      481 ggatgttgcc gtcctctttg aaatcaatgc ctttcagttc aatacggttc accagagtat
      541 caccttcaaa tttaacctct gcacgggttt tgtaggtgcc atcgtctttg aaagaaatgg
      601 tgcgctcctg tacataacct tccggcattg cagatttgaa gaaatcatgc tgcttcatgt
      661 gatccgggta acgagaaaaa cactgaacac cataggtcag ggtagtcacc agagtcggcc
      721 atggaaccgg cagtttaccg gtagtgcaga tgaatttcag ggtcagttta ccgttggttg
      781 catcaccttc accttcacca cgaacagaga atttgtggcc gttaacatca ccatccagtt
      841 caaccaggat cggaacaaca ccggtgaaca gttcttcacc tttacgcata tgtttttcct
      901 cctaaacatg cgtcgcggcc taggtcgtgc aattatacct ggccgcgaga gccttgtcaa
      961 tgggcttaat taagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     1021 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     1081 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     1141 ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg
     1201 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     1261 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     1321 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     1381 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     1441 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     1501 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     1561 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     1621 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     1681 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     1741 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     1801 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     1861 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     1921 cactacgtga aaggcgagat caccaaggta gtcggcaaat aagacaattg tccttcgccc
     1981 accccccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     2041 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     2101 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     2161 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccggattac
     2221 cctgttatcc ctagaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg
     2281 agctcgaatt cagattaccc tgttatccct atactggccg tcgttttaca acgtcgtgac
     2341 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     2401 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     2461 ggcgaatggg gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg ctggacgacc
     2521 tcgcggagtt ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc agcggctatc
     2581 cgcgcatcca tgcccccgaa ctgcaggagt ggggaggcac gatggccgct ttggtcggtc
     2641 ttgccttgct cgtcggtgat gtacttcacc agctccgcga agtcgctctt cttgatggag
     2701 cgcatgggga cgtgcttggc aatcacgcgc accccccggc cgttttagcg gctaaaaaag
     2761 tcatggctct gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt
     2821 ctcttcgatc ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg
     2881 gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg
     2941 ggccagctcg cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac
     3001 ggccagcagg taggccgaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct
     3061 tcgttcgtct ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt
     3121 ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag
     3181 agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc
     3241 gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg
     3301 aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac
     3361 cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaacg gccacgatgc
     3421 gtccggcgta gaggatctga agatcagcag ttcaacctgt tgatagtacg tactaagctc
     3481 tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt ttaacgaact
     3541 aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc tcatgtttca
     3601 cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact taaatagcct
     3661 ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg
     3721 tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct tagagcctct
     3781 caaagcaatt ttgagtgaca caggaacact taacggctga catgggaatt agcttcacgc
     3841 tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt
     3901 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa
     3961 aacgcaagcg caaagagaaa gc
//
