LOCUS       Exported                6271 bp ds-DNA     circular SYN 10-JAN-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Eric Schreiter
  TITLE     Direct Submission
  JOURNAL   Exported Jan 10, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    57..223
                     /label=ITR
     promoter        267..714
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      742..751
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             754..774
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             781..798
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             805..2031
                     /codon_start=1
                     /label=rsCaMPARI
                     /label=rsCaMPARI-46
                     /translation="MVSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFLYGNRVFVKYPDNIQDYFKQSFPKGYSWERSMTFEDGGICYA
                     RSDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALL
                     LEGNAHYRALSSRRKFNKTGHALRAIGRLSSGGSGGSGGSGGSDQLTEEQIAEFKEAFS
                     LFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKM
                     KDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQ
                     VNYEEFVVMMTAKCLFRTTYKAKEKGVKLPGVHYVDHTIEILSHDKDYNKVKIYEYAVA
                     HSGLPDNARR"
     misc_feature    1324..1386
                     /label=M13
     misc_feature    1423..1863
                     /label=CaM
     CDS             2041..2748
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDI
                     ALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVA
                     KYSNLGGGMDELYK"
     misc_feature    2764..3352
                     /label=WPRE
     CDS             complement(3235..3246)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3355..3371)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3401..3522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(3646..3812)
                     /label=ITR
     rep_origin      3885..4340
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4366..4470
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4471..5331
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5502..6090
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg
       61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
      121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
      181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa
      241 gatctctgca gagggccctg cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg
      301 ggtgggggtg cctacctgac gaccgacccc gacccactgg acaagcaccc aacccccatt
      361 ccccaaattg cgcatcccct atcagagagg gggaggggaa acaggatgcg gcgaggcgcg
      421 tgcgcactgc cagcttcagc accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc
      481 caccgccgcc tcagcactga aggcgcgctg acgtcactcg ccggtccccc gcaaactccc
      541 cttcccggcc accttggtcg cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg
      601 aggcgcgaga taggggggca cgggcgcgac catctgcgct gcggcgccgg cgactcagcg
      661 ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaat
      721 tcaagctgct agcaaggatc cgccaccatg ggtcccaaga agaagaggaa agtcggttct
      781 catcatcacc accaccatgg atccatggtg agtgcgatta agccagacat gaagatcaaa
      841 ctgcgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc
      901 aagccttatg agggaaaaca gaccatggat cttgaagtca aagagggcgg acctctgcct
      961 tttgcctttg atatcctgac cactgcattc ctgtacggca acagggtatt cgtgaaatat
     1021 ccagacaaca tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga
     1081 agcatgactt tcgaagacgg gggcatttgc tatgccagaa gcgacataac aatggaaggg
     1141 gacactttct ataataaagt tcgattttat ggtaccaact ttcccgccaa tggtccagtt
     1201 atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga
     1261 gtgctgacgg gtgatgtaga gatggctttg ttgcttgaag gaaatgccca ttaccgagcg
     1321 ttgtcaagcc gccgcaagtt caacaaaacc ggccatgcct tgcgtgcaat tggccgcctg
     1381 tccagcggag gctctggcgg ttccggcggt tcaggtggat cagaccagct gaccgaggag
     1441 caaattgcag aatttaagga ggcgttctct ttgtttgaca aagatggcga cggtacaatt
     1501 acaaccaagg aattgggcac agtaatgcgc tcccttggac agaatcccac tgaagccgaa
     1561 cttcaagaca tgatcaatga ggttgatgct gacggggatg ggactatcga ctttcctgag
     1621 tttcttacca tgatggctcg caaaatgaag gatacagaca gtgaggagga aattcgtgag
     1681 gcctttcgtg tgtttgataa ggacgggaac ggttatatct cagcggcgga gctgcgtcac
     1741 gttatgacca acttaggcga aaagctgact gatgaagaag tcgatgagat gatccgcgag
     1801 gcggacatcg atggtgatgg tcaagtcaat tatgaagagt ttgtggtgat gatgaccgcg
     1861 aagtgcctct tcagaactac ttacaaagct aaggagaagg gtgtcaagtt accaggcgtg
     1921 cactatgtgg accacaccat tgagatttta agccatgaca aagattacaa caaggttaag
     1981 atctatgagt atgctgttgc tcattctgga ttgcctgaca atgccagacg aggaggtagc
     2041 gtgtctaagg gcgaagagct gatcaaggaa aatatgcgta tgaaggtggt catggaaggt
     2101 tcggtcaacg gccaccaatt caaatgcaca ggtgaaggag aaggcagacc gtacgaggga
     2161 gtgcaaacca tgaggatcaa agtcatcgag ggaggacccc tgccatttgc ctttgacatt
     2221 cttgccacgt cgttcatgta tggcagccgt acctttatca agtacccggc cgacatccct
     2281 gatttcttta aacagtcctt tcctgagggt tttacttggg aaagagttac gagatacgaa
     2341 gatggtggag tcgtcaccgt cacgcaggac accagccttg aggatggcga gctcgtctac
     2401 aacgtcaagg tcagaggggt aaactttccc tccaatggtc ccgtgatgca gaagaagacc
     2461 aagggttggg agcctaatac agagatgatg tatccagcag atggtggtct gagaggatac
     2521 actgacatcg cactgaaagt tgatggtggt ggccatctgc actgcaactt cgtgacaact
     2581 tacaggtcaa aaaagaccgt cgggaacatc aagatgcccg gtgtccatgc cgttgatcac
     2641 cgcctggaaa ggatcgagga gagtgacaat gaaacctacg tagtgcaaag agaagtggca
     2701 gttgccaaat acagcaacct tggtggtggc atggacgagc tgtacaagta aaagcttatc
     2761 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2821 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     2881 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     2941 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3001 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3061 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3121 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     3181 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3241 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3301 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     3361 cgtcgacctc gacccgggcg gccgcttcga gcagacatga taagatacat tgatgagttt
     3421 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     3481 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     3541 cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc
     3601 tacaaatgtg gtaaaatcga taaggatctt cctagagcat ggctacgtag ataagtagca
     3661 tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct
     3721 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3781 ccgggcggcc tcagtgagcg agcgagcgcg cagctggcgt aatagcgaag aggcccgcac
     3841 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     3901 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3961 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4021 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4081 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4141 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4201 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4261 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4321 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4381 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4441 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4501 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4561 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4621 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4681 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4741 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4801 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4861 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4921 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4981 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5041 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5101 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5161 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5221 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5281 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5341 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5401 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5461 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5521 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5581 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5641 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5701 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5761 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5821 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5881 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5941 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6001 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6061 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6121 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6181 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6241 cgcagcgagt cagtgagcga ggaagcggaa g
//
