LOCUS       pSEVA6213S              4451 bp    DNA     circular UNA 10-JAN-2019
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...4o-9mo0nx3
VERSION     urn.local...4o-9mo0nx3
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        <10..74
                     /source="PEM7-Promoter.seq"
                     /modified_by="User"
                     /label="Pem7 promoter"
     misc_feature    81..84
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="RBS"
     gene            95..805
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="I-SceI"
     terminator      901..1003
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /label="T0"
     CDS             1247..1780
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /label="acc3 CDS"
     gene            1247..1780
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="acc3 gene"
     misc_feature    1856..2101
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /label="oriT"
     CDS             complement(2160..3308)
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="trfA CDS"
     gene            complement(2160..3308)
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /label="trfA gene"
     terminator      4346..4450
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /label="T1"
ORIGIN      
        1 ttaattaatg tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
       61 tgaggaacta aacccctagg ggagtcatga acatatgaaa aacatcaaaa aaaaccaggt
      121 aatgaacctg ggtccgaact ctaaactgct gaaagaatac aaatcccagc tgatcgaact
      181 gaacatcgaa cagttcgaag caggtatcgg tctgatcctg ggtgatgctt acatccgttc
      241 tcgtgatgaa ggtaaaacct actgtatgca gttcgagtgg aaaaacaaag catacatgga
      301 ccacgtatgt ctgctgtacg atcagtgggt actgtccccg ccgcacaaaa aagaacgtgt
      361 taaccacctg ggtaacctgg taatcacctg gggcgcccag actttcaaac accaggcttt
      421 caacaaactg gctaacctgt tcatcgttaa caacaaaaaa accatcccga acaacctggt
      481 tgaaaactac ctgaccccga tgtctctggc atactggttc atggatgatg gtggtaaatg
      541 ggattacaac aaaaactcta ccaacaaatc gatcgtactg aacacccagt ctttcacttt
      601 cgaagaagta gaatacctgg ttaagggtct gcgtaacaaa ttccaactga actgttacgt
      661 aaaaatcaac aaaaacaaac cgatcatcta catcgattct atgtcttacc tgatcttcta
      721 caacctgatc aaaccgtacc tgatcccgca gatgatgtac aaactgccga acactatctc
      781 ctccgaaact ttcctgaaat aataagaatt cgagctcggt acccggggat cctctagagt
      841 cgacctgcag gcatgcaagc ttgcggccgc gtcgtgactg ggaaaaccct ggcgactagt
      901 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
      961 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc caataattac
     1021 gatttaaatt tgacataagc ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc
     1081 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     1141 ttcatggctt gttatgactg tttttttgta cagcctatgc ctcgggcatc caagcagcaa
     1201 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     1261 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaggtggct caagtatggg
     1321 catcattcgc acatgtaggc tcggccctga ccaagtcaaa tccatgcggg ctgctcttga
     1381 tcttttcggt cgtgagttcg gagacgtagc cacctactcc caacatcagc cggactccga
     1441 ttacctcggg aacttgctcc gtagtaagac attcatcgcg cttgctgcct tcgaccaaga
     1501 agcggttgtt ggcgctctcg cggcttacgt tctgcccaag tttgagcagc cgcgtagtga
     1561 gatctatatc tatgatctcg cagtctccgg agagcaccgg aggcagggca ttgccaccgc
     1621 gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt gcttatgtga tctacgtgca
     1681 agcagattac ggtgacgatc ccgcagtggc tctctataca aagttgggca tacgggaaga
     1741 agtgatgcac tttgatatcg acccaagtac cgccacctaa caattcgttc aagccgagat
     1801 cggcttcccg gccgcggagt tgttcggtaa attggacaac ggtccgcgcg ttgtcctttt
     1861 ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt
     1921 tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac
     1981 ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca
     2041 ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccgt
     2101 aggccggccg cgatgcaggt ggctgctgaa cccccagccg gaactgaccc cacaaggccc
     2161 tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct
     2221 cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc
     2281 gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg
     2341 aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat
     2401 ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa
     2461 cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc
     2521 aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat
     2581 tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg
     2641 tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc
     2701 tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg
     2761 tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg
     2821 cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc
     2881 cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc
     2941 agcaacgcgg cctgcttggc ttcgctgacc tgttttgcca ggtcctcgcc ggcggttttt
     3001 cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc
     3061 gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg
     3121 ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac tatcgagccg
     3181 acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca
     3241 tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc
     3301 cgattcattc accctccttg cgggattgcc ccggaattaa ttccccggat cgatccgtcg
     3361 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     3421 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
     3481 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
     3541 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
     3601 accgtttctg cggactggct ttctacgtgg ctgccatttt tggggtgagg ccgttcgcgg
     3661 ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga
     3721 gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta
     3781 aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg
     3841 aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat
     3901 gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc
     3961 cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg
     4021 cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag
     4081 gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg
     4141 ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag
     4201 ttttccgcga ggtatccaca acgccggcgg ccctacatgg ctctgctgta gtgagtgggt
     4261 tgcgctccgg cagcggtcct gatcccccgc agaaaaaaag gatctcaaga agatcctttg
     4321 atcttttcta cggcgcgccc agctgtctag ggcggcggat ttgtcctact caggagagcg
     4381 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
     4441 atttgatgcc t
//
