LOCUS       pSEVA1213S              4673 bp    DNA     circular UNA 10-JAN-2019
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...4q-9mo0nx3
VERSION     urn.local...4q-9mo0nx3
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        <9..73
                     /source="PEM7-Promoter.seq"
                     /modified_by="User"
                     /label="Pem7 promoter"
     misc_feature    80..83
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="RBS"
     gene            94..804
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /label="I-SceI"
     terminator      900..1002
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /label="T0"
     misc_feature    1030..2072
                     /Original_Bases="TGACATAAGCCTGTTCGGTTCGTAAACTGTAATGCAAGTAG
                     CGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCG
                     CAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGTACAGCCTATGCCTCGGGC
                     ATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAAC
                     GATGTTACGCAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTA
                     GGTGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAAT
                     CCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTC
                     CCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATC
                     GCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGC
                     CCAAGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGAGA
                     GCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAAC
                     GCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGG
                     CTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAG
                     TACCGCCACCTAACAATTCGTTCAAGCCGAGATCGGCTTCCCGGCCGCGGAGTTGTTC
                     GGTAAATTGGACAACGGTCCGCGCG"
                     /label="TGACATAAGCCTGTTCGGTTCGTAAACTGTAATGCAAGTAGCGTATGCGC
                     TCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGG
                     TTTTCATGGCTTGTTATGACTGTTTTTTTGTACAGCCTATGCCTCGGGCATCCAAGCA
                     GCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACG
                     CAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGTGGCTCA
                     AGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGG
                     CTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCA
                     GCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCT
                     GCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAAGTTTG
                     AGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGAGAGCACCGGAG
                     GCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGT
                     GCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATA
                     CAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCAC
                     CTAACAATTCGTTCAAGCCGAGATCGGCTTCCCGGCCGCGGAGTTGTTCGGTAAATTG
                     GACAACGGTCCGCGCG"
                     /note="Geneious type: Editing History Replacement"
     CDS             1163..2023
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /label="bla CDS"
     gene            1163..2023
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /label="bla gene"
     misc_feature    2078..2323
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /label="oriT"
     CDS             complement(2382..3530)
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /label="trfA CDS"
     gene            complement(2382..3530)
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /label="trfA gene"
     terminator      4568..4672
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /label="T1"
ORIGIN      
        1 ttaattaagt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
       61 gaggaactaa acccctaggg gagtcatgaa catatgaaaa acatcaaaaa aaaccaggta
      121 atgaacctgg gtccgaactc taaactgctg aaagaataca aatcccagct gatcgaactg
      181 aacatcgaac agttcgaagc aggtatcggt ctgatcctgg gtgatgctta catccgttct
      241 cgtgatgaag gtaaaaccta ctgtatgcag ttcgagtgga aaaacaaagc atacatggac
      301 cacgtatgtc tgctgtacga tcagtgggta ctgtccccgc cgcacaaaaa agaacgtgtt
      361 aaccacctgg gtaacctggt aatcacctgg ggcgcccaga ctttcaaaca ccaggctttc
      421 aacaaactgg ctaacctgtt catcgttaac aacaaaaaaa ccatcccgaa caacctggtt
      481 gaaaactacc tgaccccgat gtctctggca tactggttca tggatgatgg tggtaaatgg
      541 gattacaaca aaaactctac caacaaatcg atcgtactga acacccagtc tttcactttc
      601 gaagaagtag aatacctggt taagggtctg cgtaacaaat tccaactgaa ctgttacgta
      661 aaaatcaaca aaaacaaacc gatcatctac atcgattcta tgtcttacct gatcttctac
      721 aacctgatca aaccgtacct gatcccgcag atgatgtaca aactgccgaa cactatctcc
      781 tccgaaactt tcctgaaata ataagaattc gagctcggta cccggggatc ctctagagtc
      841 gacctgcagg catgcaagct tgcggccgcg tcgtgactgg gaaaaccctg gcgactagtc
      901 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
      961 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aggggtcccc aataattacg
     1021 atttaaatta gtagcccgcc taatgagcgg gctttttttt aattccccta tttgtttatt
     1081 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1141 ataatattga aaaaggaaga gtatgagcat tcagcatttt cgtgtggcgc tgattccgtt
     1201 ttttgcggcg ttttgcctgc cggtgtttgc gcatccggaa accctggtga aagtgaaaga
     1261 tgcggaagat caactgggtg cgcgcgtggg ctatattgaa ctggatctga acagcggcaa
     1321 aattctggaa tcttttcgtc cggaagaacg ttttccgatg atgagcacct ttaaagtgct
     1381 gctgtgcggt gcggttctga gccgtgtgga tgcgggccag gaacaactgg gccgtcgtat
     1441 tcattatagc cagaacgatc tggtggaata tagcccggtg accgaaaaac atctgaccga
     1501 tggcatgacc gtgcgtgaac tgtgcagcgc ggcgattacc atgagcgata acaccgcggc
     1561 gaacctgctg ctgacgacca ttggcggtcc gaaagaactg accgcgtttc tgcataacat
     1621 gggcgatcat gtgacccgtc tggatcgttg ggaaccggaa ctgaacgaag cgattccgaa
     1681 cgatgaacgt gataccacca tgccggcagc aatggcgacc accctgcgta aactgctgac
     1741 gggtgagctg ctgaccctgg caagccgcca gcaactgatt gattggatgg aagcggataa
     1801 agtggcgggt ccgctgctgc gtagcgcgct gccggctggc tggtttattg cggataaaag
     1861 cggtgcgggc gaacgtggca gccgtggcat tattgcggcg ctgggcccgg atggtaaacc
     1921 gagccgtatt gtggtgattt ataccaccgg cagccaggcg acgatggatg aacgtaaccg
     1981 tcagattgcg gaaattggcg cgagcctgat taaacattgg taaaccgata caattaaagg
     2041 ctccttttgg agcctttttt tttggacgac ccttgtcctt ttccgctgca taaccctgct
     2101 tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat
     2161 tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga
     2221 taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc
     2281 tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaggccggc cgcgatgcag
     2341 gtggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc
     2401 aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct
     2461 tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg
     2521 aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt
     2581 cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca
     2641 gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca
     2701 cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg
     2761 gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa
     2821 taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc
     2881 ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca
     2941 tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa
     3001 atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca
     3061 gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac
     3121 aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg
     3181 gcttcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata
     3241 gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga
     3301 cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg
     3361 tcgcgctcga tcttggccgt agcttgctgg actatcgagc cgacggactg gaaggtttcg
     3421 cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc
     3481 gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct
     3541 tgcgggattg ccccggaatt aattccccgg atcgatccgt cgatcttgat cccctgcgcc
     3601 atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac
     3661 cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta
     3721 gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc
     3781 ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg
     3841 ctttctacgt ggctgccatt tttggggtga ggccgttcgc ggccgagggg cgcagcccct
     3901 ggggggatgg gaggcccgcg ttagcgggcc gggagggttc gagaaggggg ggcacccccc
     3961 ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag gtttataaat
     4021 attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc
     4081 ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag gtgcgcccct
     4141 catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc
     4201 gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca catcatctgt
     4261 gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca gctccacgtc
     4321 gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga gtcggcccct
     4381 caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc gaggtatcca
     4441 caacgccggc ggccctacat ggctctgctg tagtgagtgg gttgcgctcc ggcagcggtc
     4501 ctgatccccc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggcgcgc
     4561 ccagctgtct agggcggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
     4621 ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cct
//
