LOCUS       Exported               14573 bp ds-DNA     circular SYN 10-JAN-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14573)
  AUTHORS   Fern Sha
  TITLE     Direct Submission
  JOURNAL   Exported Jan 10, 2019 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14573
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     exon            3052..3058
                     /label=elavl3 Exon 2
     CDS             3064..3117
                     /codon_start=1
                     /label=NES
                     /translation="MLQNELALKLAGLDINKT"
     CDS             3127..3144
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3151..4377
                     /codon_start=1
                     /label=rsCaMPARI
                     /label=rsCaMPARI-46
                     /translation="MVSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFLYGNRVFVKYPDNIQDYFKQSFPKGYSWERSMTFEDGGICYA
                     RSDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALL
                     LEGNAHYRALSSRRKFNKTGHALRAIGRLSSGGSGGSGGSGGSDQLTEEQIAEFKEAFS
                     LFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKM
                     KDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQ
                     VNYEEFVVMMTAKCLFRTTYKAKEKGVKLPGVHYVDHTIEILSHDKDYNKVKIYEYAVA
                     HSGLPDNARR"
     misc_feature    3670..3732
                     /label=M13
     misc_feature    3769..4209
                     /label=CaM
     CDS             4387..5094
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDI
                     ALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVA
                     KYSNLGGGMDELYK"
     polyA_signal    5139..5273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     complement(5712..5911)
                     /label=Tol2_LA-200
     primer_bind     complement(5945..5961)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5998..6016)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6037..6053)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6061..6077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6085..6115)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6130..6151
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6439..7027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7198..8058)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8059..8163)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8100..8128
                     /label=Amp promoter
                     /note="Amp promoter"
     misc_recomb     complement(8696..8845)
                     /label=Tol2_RA_150
     promoter        8924..11682
                     /label=HuC promoter
     exon            11683..12059
                     /label=elavl3 Exon 1
     intron          join(12060..14573,1..3052)
                     /label=Intron 1
ORIGIN
        1 ccgaaattag ccaaattcac caattaaaaa aaaaataagc caacaaaaaa gaagggttta
       61 tccgttcagt tttgacttgt gtcctgttgt tttacagctg ccactgtgat cccttaagct
      121 gcagtgagag tgtggctaat gccttttgtt taagataatc ctgtaatctg ttaccgaaac
      181 ggcctattga caagccggca ttcacatttc agtcaggagg cccgtccaga catgtacatt
      241 tatgaattac gaatgataaa attaagatct gcattaggcg tcagaaatgt cacgaacacc
      301 cattcatttc ccacagccaa taatggatca tgctgggaag cgctatcctc gcagtctcaa
      361 aattaatgtt gacatgttgc gtagagctac agattagatc agccatatgc tttatgtgtt
      421 tacctcagcg gatcttcagc aagatgtgtt tattttaaga aaatggcttc ctcgctgcct
      481 gcacaggggc attaaggaag tgacgtgagc gtcctcacag tcaaattact ttatctcatg
      541 ctgctttcag gcctctgctt attttattat tattatttta aattaggggg aatcacggtg
      601 gcgttgtggg tagggtagcg cgatcacctt acagcaagaa ggtcgctggt tcgagctctg
      661 gctgggtcag ttggcatttc tgtgtggagt ttaaattgaa ttgaaattga ataaactaaa
      721 ttggccctag tgtatgtgtg tgaatgtaag tgtgcatggg tgtttcccag tgttgggttt
      781 gactggaaga gcatccgctt cgtaaaacat atgctggata agttggcggt tcatttcact
      841 gtggtgaccg ctgattaata aagggactaa gctgaaaatg aaatgaatta atacatttta
      901 aatgagcttt tgctgacatg tttatgatta caaatagtga aattgtatgt taatttacga
      961 taagtgcatt gttctaaatt catttttatg tagcagctgg tttttcttcc atttgtgtca
     1021 ttcaacagaa catttttcta aaaaagtgaa cttaaatttt ctcctccctc tgacaaggct
     1081 tgatggtaat ttcagccgac tctaattaca gactcactaa aaggaatcgg tgcagtcatt
     1141 tatctttatc agcgcgctgg tgggaaggta ataggttttg cttatgagtt gtttgcgtgc
     1201 gagtctgggt ccttgtgtag gcgatgctga tgcactgcat taaatattga gggagaagtc
     1261 gctcaaaaat agcaaagtac aggctggggc aggaacgtca aagcccgctc agtggccacg
     1321 tttcttgttt cgtaagtcct atttttaata gcgataattc aggctcagta gaaggaactc
     1381 cagcactata agcagtctct cgcctgtctc ggcgagttat gtattttggg atgaagtgct
     1441 cttccacctc tacctctgct gtgctggcgc cagtatctgg gctgtaagat agtgtacgtg
     1501 tcagtctgtg tggccgaaga gcctgtgtgt tgtgtaactc actacattcg tcgctgtgga
     1561 tggctgactt gccatgggga ggtttacttc agttcacgaa tcacattcgc cacgatcttg
     1621 tcactttgtg tcaagactcg ctctctgtgt gtcaacagct ctgctgtttg aggttgaaat
     1681 tctgaacttg agatatatcc caggatatat tagggctact gccaacggaa aaagcaaatg
     1741 gtgccatggc catttgattg atcaaacaaa cacccttttg cttgggtgac agtgaaatgt
     1801 cagatccatg tttgtactgc tttaataact tgcctctctg agctttctcc ttgtcagtgt
     1861 caatttgcct gagatataaa ttccggttgc cccgaaagct tattttatta aaaaaaagct
     1921 aacatgcact catacaacgt gcgcacatac atgtgaggct tgctaaaagt caagaagcaa
     1981 gccaatagca agagtcgtca gactgctgtt acctgggcaa cctggttaga ggtaaggctc
     2041 aaggcaagaa taaatcattc cactgaagag acacagcgtt acagcgagat gagagcaaga
     2101 ggcgatagtg aagggagaac aaaagaacag cctacccaca ctagtgcaga tctacctgtt
     2161 tgagctactg attgttcagt ttgattagat atcagacaat tctgctatct ccaattgcat
     2221 tgctgcatga attcctagaa atcattttaa tcaccaaaaa ctagtaggga cacttgtggc
     2281 gtatatgatt ctgcctgttg attgtgggga tccaacagaa agatcatgaa tgcattgtta
     2341 attaagtcgg tgagacaccc tgttccaccc taggggccgt tctggggaaa gagtgctttc
     2401 agtcagctaa agatgactaa tatgtgaaca tatattacta cacatgccaa tttgtacatt
     2461 ctggatagtt accagccctg ggaaaacatc gaaaaaaata aatcaacata gataaaattg
     2521 gcaaatccct tctggtacga tgcagagtgc ttgctctgag aacttgtgca ttgtgacaga
     2581 aagcgaccgt taaaaactga aagtagcatc tacaacctgc atacatctgt tctttgaaaa
     2641 tcccctgtag gccaaattaa gcatcacgct gtgctccaca gcaacacgga tgaaccgaaa
     2701 acccagacac aaacgcacac acttttcaca taaacaaggt ttagttatgc tagctatgct
     2761 ttttcgtcct ccctttgtca tcagcggcga gtgacgtaac acagtttgac cctggacagc
     2821 gtaaaggggg ggttgggaat gagtgaaagg actgtgacat ctgcagggcc aggggctgat
     2881 ggagagagct gaagtgaggg aggtcagagg aggaggaagg gtgggatatt gtcctcctca
     2941 ggccctatcc ctctcagacg cattgatttt taacctctca gcgagaatgc caagcattac
     3001 acccctcaaa ttatggttcc tcacccaacc tgttatattt tccacctgca gataattacc
     3061 ggtatgctgc agaacgagct tgctcttaag ttggctggac ttgatattaa caagactgga
     3121 ggttctcatc atcaccacca ccatggatcc atggtgagtg cgattaagcc agacatgaag
     3181 atcaaactgc gtatggaagg caacgtaaac gggcaccact ttgtgatcga cggagatggt
     3241 acaggcaagc cttatgaggg aaaacagacc atggatcttg aagtcaaaga gggcggacct
     3301 ctgccttttg cctttgatat cctgaccact gcattcctgt acggcaacag ggtattcgtg
     3361 aaatatccag acaacataca agactatttt aagcagtcgt ttcctaaggg gtattcgtgg
     3421 gaacgaagca tgactttcga agacgggggc atttgctatg ccagaagcga cataacaatg
     3481 gaaggggaca ctttctataa taaagttcga ttttatggta ccaactttcc cgccaatggt
     3541 ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt
     3601 gatggagtgc tgacgggtga tgtagagatg gctttgttgc ttgaaggaaa tgcccattac
     3661 cgagcgttgt caagccgccg caagttcaac aaaaccggcc atgccttgcg tgcaattggc
     3721 cgcctgtcca gcggaggctc tggcggttcc ggcggttcag gtggatcaga ccagctgacc
     3781 gaggagcaaa ttgcagaatt taaggaggcg ttctctttgt ttgacaaaga tggcgacggt
     3841 acaattacaa ccaaggaatt gggcacagta atgcgctccc ttggacagaa tcccactgaa
     3901 gccgaacttc aagacatgat caatgaggtt gatgctgacg gggatgggac tatcgacttt
     3961 cctgagtttc ttaccatgat ggctcgcaaa atgaaggata cagacagtga ggaggaaatt
     4021 cgtgaggcct ttcgtgtgtt tgataaggac gggaacggtt atatctcagc ggcggagctg
     4081 cgtcacgtta tgaccaactt aggcgaaaag ctgactgatg aagaagtcga tgagatgatc
     4141 cgcgaggcgg acatcgatgg tgatggtcaa gtcaattatg aagagtttgt ggtgatgatg
     4201 accgcgaagt gcctcttcag aactacttac aaagctaagg agaagggtgt caagttacca
     4261 ggcgtgcact atgtggacca caccattgag attttaagcc atgacaaaga ttacaacaag
     4321 gttaagatct atgagtatgc tgttgctcat tctggattgc ctgacaatgc cagacgagga
     4381 ggtagcgtgt ctaagggcga agagctgatc aaggaaaata tgcgtatgaa ggtggtcatg
     4441 gaaggttcgg tcaacggcca ccaattcaaa tgcacaggtg aaggagaagg cagaccgtac
     4501 gagggagtgc aaaccatgag gatcaaagtc atcgagggag gacccctgcc atttgccttt
     4561 gacattcttg ccacgtcgtt catgtatggc agccgtacct ttatcaagta cccggccgac
     4621 atccctgatt tctttaaaca gtcctttcct gagggtttta cttgggaaag agttacgaga
     4681 tacgaagatg gtggagtcgt caccgtcacg caggacacca gccttgagga tggcgagctc
     4741 gtctacaacg tcaaggtcag aggggtaaac tttccctcca atggtcccgt gatgcagaag
     4801 aagaccaagg gttgggagcc taatacagag atgatgtatc cagcagatgg tggtctgaga
     4861 ggatacactg acatcgcact gaaagttgat ggtggtggcc atctgcactg caacttcgtg
     4921 acaacttaca ggtcaaaaaa gaccgtcggg aacatcaaga tgcccggtgt ccatgccgtt
     4981 gatcaccgcc tggaaaggat cgaggagagt gacaatgaaa cctacgtagt gcaaagagaa
     5041 gtggcagttg ccaaatacag caaccttggt ggtggcatgg acgagctgta caagtaaccg
     5101 gtggatcctg gccgtcgttt tactgtacca tcgatgatga tccagacatg ataagataca
     5161 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     5221 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     5281 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     5341 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagatcag atctgcgaag
     5401 atacggccac gggtgctctt gatcctgtgg ctgattttgg actgtgctgc tcgcagctgc
     5461 tgatgaatca catacttcct ccattttctt ccactgattg actgttataa tttccctaat
     5521 ttccaggtca aggtgctgtg cattgtggta atagatgtga catgacgtca cttccaaagg
     5581 accaatgaac atgtctgacc aatttcatat aatgtgaaaa cgattttcat aggcagaata
     5641 aataacattt aaattaaact gggcatcagc gcaattcaat tggtttggta atagcaaggg
     5701 aaaatagaat gaagtgatct ccaaaaaata agtacttttt gactgtaaat aaaattgtaa
     5761 ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa aagtattgat
     5821 ttttaattgt actcaagtaa agtaaaaatc cccaaaaata atacttaagt acagtaatca
     5881 agtaaaatta ctcaagtact ttacacctct ggttcttgac cccctacctt cagcaagccc
     5941 agcagatcca ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct
     6001 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     6061 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6121 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6181 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6241 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6301 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6361 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6421 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6481 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6541 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6601 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6661 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6721 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6781 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6841 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     6901 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6961 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7021 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7081 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7141 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7201 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7261 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7321 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7381 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7441 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7501 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7561 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7621 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7681 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7741 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7801 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7861 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7921 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7981 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8041 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8101 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8161 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     8221 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     8281 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     8341 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     8401 gatggcccac gctggctaag aactcatcag cctccccggt ccatctaccc acgtaccaat
     8461 gcaccaattg gccacaatga cggctactac atggtgccat tccttcctct ttataggaat
     8521 ggagactacc tcctgtccaa caaggctctt ggatacgagt acgcctacct gttggaccca
     8581 ggtcattgca caacaccaga aatgccctct gatctgcaaa agacgtgaat atctgttcag
     8641 acacccatat ccactctgtt ccacacaggt cagaggtttg tccaggagtt cttgacagag
     8701 gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac aagtacttag ggaaaatttt
     8761 actcaattaa aagtaaaagt atctggctag aatcttactt gagtaaaagt aaaaaagtac
     8821 tccattaaaa ttgtacttga gtattaagga agtaaaagta aaagcaagaa agaaaactag
     8881 agattcttgt ttaagcttcc cgggtgtcga cgaattctcg agctattcct aaagaccttg
     8941 ggtgaccaaa atcttatttt aataaataaa actgtttatt aaaacttttt tgtttcaaag
     9001 aaccatatgt atagtgaaat ttataaaaat atcaattttt aaaaagctgg tgtactcatt
     9061 tatgttatga actctaaaac catatactga ctgcaagtga tgatgtatag agtgatgttt
     9121 acgagtaaac atatttagtt gtatacatcc tactgagcac attttgatgt atgaaataac
     9181 attacaagct ttatccaaat taagccattt taaaacactg ccaattgaaa atacaaatcc
     9241 tggaaaaaat cgtctttagc gcagtcattt gagccatcct aatccgttac ctcagaccat
     9301 aataagaagg gataacacta gctgtagcaa tggaacacat ctgtttcaca caatcatatc
     9361 tcctgcgccg gtgctaagca gattcagcgt gatcataaca tgctttccac tcataaatgt
     9421 aaatttacaa tttgcacatg taaaacagac acttttgaga tattggataa aaaaacaaga
     9481 gtatattgct tagtttcatc caccagtcat ccccacagcg tttggaaggc cataaaaagt
     9541 gtctaaaatc aatgatcatt gaaagagcac aagagagact cttacgctgt aatgccactg
     9601 gggacaaaag tgacagtctc ttaatgggct cttctggagg ggctcctgaa cattaaaaat
     9661 tatcagcgaa attaccgaaa gagcttcaag caactggcat gcttgatcct ctgcgtcggg
     9721 gcggtgaata ggtgcttcag atgccctctt acccacgggc tggattcagc tgccccgcta
     9781 ccagcggaga ccccctaatg agcctctgca attaagttta ttcatgttaa gtgtgaacgg
     9841 ggtgcgtgcg gaactgtggg cagctaacag acctgggttc tttgtgccac aagtgctgcc
     9901 tttattcggc tcacaaagca gaaaacaaca cccgcaccta taatggcgcc ctcggctggg
     9961 tctaagaaac gtggcgagtt gacagagcag agtgggcggg gttaagacag actgacagcg
    10021 ggacccatct ccatcctctt attaacgctt aacgagtgcc ttcctcatgc aatattcatc
    10081 gccactaata tcatccaagc tctgagctga gctggccact tatgtaaggc aattatgtaa
    10141 aatatcagac agggcccaca ctcagaatct gactggggta gagacgcggg acgagaaccg
    10201 agagcaagaa ctgaaagtga aagtgaccac taaagggagg agaggacaga ggggcaggat
    10261 gtgtcaagat taccagagaa cacttggcca gaaatgcgca accattggag ctctccggat
    10321 tacccaaagg ttaacgagtt tgaacgcctc tgcccactcg cccatctctg atggtttccc
    10381 aagaactcct caagcaaaat atatataatt gtgtgtatta tgcacagaca cgagaaaatg
    10441 ctgtttttct gatctgcatt acagcacatt tgcccgccaa cgacaatacc acccactcgg
    10501 tacctcgctg actcctgatg cctgatacct gcgcggtgac tgtctacaat ctgcataatc
    10561 aagagaagtt gtgttgaaga cgagcgccac acaaccgttt ccacaaggtc acccaaggcc
    10621 ggtgcagatg taggtgaggt ctccataaac agactgaaat aaacacatcc tccgctggga
    10681 acaacaaccc cctcacgcct catgcatttc cataagccta catgcatctc ttccaactta
    10741 tggagactcg cacctaccaa catccgcaca acaaagatat acagagcgcg ctccctcagg
    10801 tcaaggccct gtgggggtct gtgcagaaat aggtcatttg tcacacatca agtcctgggg
    10861 caggagatgc attatagatg agaccaaaca gcctgtctcg gtgagctcta cccactccct
    10921 gagactagaa atgggggaag ggagcttgag ataacaaccg ctgcaatcac tgtgtcgatg
    10981 tttaatatca gcaccaaccg ggaacaataa ggagatgggt gcattcatgt tcacatctta
    11041 ccagtcaagt atcatcgaac cggcttgata accacacctc gtgtaatagc tgagcagata
    11101 gttgtcattt taaagcgttg gcctttgtcg attatgtaat gcgcacattc aacacatggt
    11161 aatatagaaa cggttatgtc gaggttgttt tgtccagaga tgaccttcac acagttacag
    11221 ccgctctgca tccacacaaa tggaggactt aatcgtggac tgcattctta gaaatgatct
    11281 acaaagacaa ataatgtgaa atcaagaaag gacaaaattt aagtaagggg atgagggaga
    11341 gagagaacga ggggcaagga gaaagcatgg ctcctgtctt tttctgcacc catctgttcg
    11401 gagtgcaggt ggagctctat tcactcagct ctgcatgtgt gtttgggggg ggcaggaaga
    11461 aagggagggc aaaaggaaga gtggagagat ggtgggggct ggagggatgg ggggttctcg
    11521 gtgatctctc ctgaagggga taatgggaga gcagcgcttt gcaatggctg ccatgtagta
    11581 ccctccctgc acaattagcc aatcagcagc aagctctgcc agccagaagg acacataaaa
    11641 gaagaacatt gcagcagagg cacagaagga gcctgcgagg agctgggaaa tacacacaca
    11701 acagcagaac cacaacaccc tcccctggac acaccctact ggggatcact gcttttcttt
    11761 ttttctgaac catcgcccac gccacacgga gagaaatctc tctctcatca tcatcctgaa
    11821 gaaaaccccc ttatcctcat tttcacactg ctgaggaaaa cctacaatcg cacgggctga
    11881 gatttcctgg cgaagactgt cctttttcct ttttcttttt ttttcttttc ctttggaaac
    11941 tgacatttgc atttctccat tccaagccac ggcgtaataa tatctgcaat ccagcctgaa
    12001 gacctgcaaa tcgaaggacc tagatcacta tcatctttgt acgtcaagaa tggttactgt
    12061 acgtataacc tttctttctt ttgctctgac caatatgaaa cactaaaatc gattcgagca
    12121 gcctctcagc atcaattaca gtgcgtgaaa aacattcaaa tagaggcagc aaatattaca
    12181 tgtgaaaata caggctggct aaatccaagc taatttagaa atgtggtcaa aacgcatact
    12241 ggcacgtcta atcgcggatt cagtaaacaa gattaacgat tagcccagtg tataagtcat
    12301 atgatacagg catgcgcgag agcatcgcta cacccgagct ggcttcattt tcggaggaaa
    12361 atcaaaacat tgctttctcc tgccgtgcga accattcgtc ataaaccgta atacgcaaca
    12421 tacatttatt actacatccg ttaattagcg ataattagcc gttattaaca aagagcgctg
    12481 aggaattcct ctcaaatagc ggaggtgcgg cggaggcaga ggggcgtaaa agggcacatg
    12541 cgtgcctggc tcaaaaaagg atgctccaga ctgaaactca gagacaaaac acgatgcctc
    12601 ggaggctgag agcccatcga gaggaaaggc aaagaaaagg gtcgagtcac tcagaggggg
    12661 aggggagata ttgtgcatcc attggtttga attgaagcag gcagaataaa aatccttgcg
    12721 attatgcttc gtgagagatc gcgagagagg aaaaggctaa atgccacgta tgcaaatgga
    12781 taaatgtcat ctatttcttc gcgagtagac attttgtgga caacgagagt gtaaataagc
    12841 gatatgaggg gaatacaaag gccttgaggg aaagcgtttt gggcgtgtct tgtcgaaaag
    12901 aagacagcga tggcacgcgc tcagaaccac catcttcacg tagataatcc gcgtgaaaac
    12961 aataacaaaa ctgctcattt taacacgagc aggtgtttga aaccacccaa aaaagattgt
    13021 ctaaaatatt aagattaatt aatgacgttt aaatgtccag gcctgatata aatgaagcgt
    13081 gtagtaagag taatcgcctt tgtttctttt cagagatacg ttttcagaga ttcagggaat
    13141 tatatcttca ataaaataat gtttgatttc attattttaa agctttttaa gaagtaaaaa
    13201 tacacattat tcatttacat tttattttat ttaaaggcct atggtcttga aaataataag
    13261 tggaatatat aggctatata ttcgtctaag gacagccggt gttattttat agtgacctca
    13321 gacaaaagcc gagacaaaac gaggcgcttg cctttcattt taatccttaa tagacggtgt
    13381 gatgaatgaa tgaatgaatg aataaatgaa tgaatgacgg gcccttcggc atgttgttcc
    13441 gcagtcctcc gtgagcacga gtctctaccg cagtgccaag cacataaaac cgttgagtca
    13501 gataaccttc tccaggcgcg tatacacaat tcaaggacaa gcacatcaca actatacaca
    13561 acacaacaca accgcctaaa cgcacgagct catcaatcgc aatttaaagg catgtcagtc
    13621 aaagaaaaag gcggcgttat ttaaaaccaa tgttcaacaa tatttgtatg ctgactagcc
    13681 cgggcgcgca cgctataaga tcacaaggcc ttgttttttc cctttattca aaatatcatt
    13741 aattattaca ggtcataaaa caaaacaatg caaattacaa tgatttaaag actattgatt
    13801 taacaataat cagttaatta tagaataata ttataatatt ttacatacaa atgaaagatg
    13861 acattatttg atgacattgt aattaaaata tatgcaatat atttttacgt ttttattaat
    13921 taggctatta ctattattat aattatcatt atttttatta ttattattat tattaaatag
    13981 tcttgcgaaa taagagtgaa ggcagttgca atgttcattg ttcccggaga gtcccgcagc
    14041 ctctggcctc aacacaaagc gctactgttt acattaatat tcatagtgct gccatggtct
    14101 ctgtagagga ggaacgggtg gctcttttgt tcgctagtgt caatacgttt attcagcaaa
    14161 aagcagctcg gatttgtgaa tgcccaacaa gcacacgcgc actccaatca aaggacgaga
    14221 ggataacgat tttaaacgat tcgcaaacgt gtttatttgt aaagacgaca tcaagagctt
    14281 aatgtccact tgaaaagaaa taaatatgcc cgcggcgcca aggatatttt gaatgtagtc
    14341 gttcatggtg agccactccc gaattcagca ctctggagag cgaccaaaga ggaatagcgg
    14401 aatagcaact acttgagcca tttctctccg tttgatggcg ttcatgatta aaaatataat
    14461 cacgtgattt tttatacata tataaatata tacataaagt aacgctttcc ttttgagaat
    14521 acataaatat ttattcgcgt gataaatcaa tgatcttata tttatttgca cga
//
