LOCUS       Exported                5327 bp ds-DNA     circular SYN 10-JAN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pROP-IP-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 10, 2019 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      495..589
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(661..1371)
                     /codon_start=1
                     /note="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    1372..1404
                     /note="RBS"
     misc_feature    complement(1405..1452)
                     /note="BBa_J23107"
     terminator      1537..1566
                     /note="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     promoter        1699..1727
                     /note="BBa_J23101"
     misc_feature    1736..1751
                     /note="ROP IP"
     RBS             1762..1776
     CDS             1783..2499
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /note="GFP"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2559..2645
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2750..3541)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="SmR"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(4110..5060)
                     /codon_start=1
                     /gene="rep101"
                     /product="protein needed for replication with the pSC101 
                     origin"
                     /note="Rep101"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5108..3
                     /note="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc
       61 tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa
      121 atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg
      181 acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc
      241 gccttttatg gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct
      301 tctcagggcg ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc
      361 agttcctgcc ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc
      421 agactggcta atgcacccag taaggcagcg gtatcatcaa caggcttacc cgtcttactg
      481 tccctagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
      541 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
      601 gtaaacttgg tctgacagct ctagctccgg caaaaaaacg ggcaaggtgt caccaccctg
      661 ttatttgtac agctcatcca tgccaccggt agaatgacga ccctccgcgc gctcatattg
      721 ctctacgatc gtataatctt cattatgaga ggtgatgtcc agtttaatat tcacattgta
      781 cgcgccaggc agctgcacag gtttcttggc tttgtacgtg gttttcactt cagcgtcata
      841 atggccgcca tctttcagtt tcaggcgctg tttaatttcg cctttcagcg caccatcttc
      901 cggatacatg cgttcgctag acgcctccca acccatcgtc tttttctgca tcaccgggcc
      961 atcagatgga aaattagtac cacgcagttt aactttatag atgaactcgc catcctgcag
     1021 ggaggagtcc tgagtgacgg tcacgacacc accatcttca aaattcatta cgcgttccca
     1081 cttgaaacct tccggaaaag acagcttcag atagtccggg atatccgctg ggtgtttaac
     1141 atacgcttta gaaccgtaca taaattgcgg gctcaggatg tcccacgcaa aaggcagcgg
     1201 gccgccttta gtcactttca gtttggcggt ctgggtgcct tcatacggac ggccctcgcc
     1261 ttcgccttcg atctcgaact cgtgaccgtt aacagaaccc tccatgtgaa ctttgaagcg
     1321 catgaactct ttaatgatag ccatgttatc ctcctcgccc ttggaaacca ttcttttcct
     1381 tcttatcctc gcttaccttc ttgctccatg ctagcataat acctagggct gagctagccg
     1441 taaaggcgcg cccccttttt ctttaaaacc gaaaagatta cttcgcgttt gccacctgac
     1501 gtctaagaaa aggaatattc agcaatttgc ccgtgccgaa gaaaggccca cccgtgaagg
     1561 tgagccagtg agttgattgc tacgtaacaa ttccgacgtc taagaaacca ttattatcat
     1621 gacattaacc tataaaaata ggcgtatcac gaggcccttt tttcgtcttc acctcgagat
     1681 gctagcaaaa aaagagtatt tacagctagc tcagtcctag gtattattac agccatgtga
     1741 aatattgagc aaaaagaatt cattaaagag gagaaaggta ccatgcgtaa aggagaagaa
     1801 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
     1861 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
     1921 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat
     1981 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
     2041 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
     2101 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
     2161 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
     2221 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
     2281 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     2341 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     2401 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     2461 gctgggatta cacatggcat ggatgaacta tacaaataaa agcttgatat cgaattcctg
     2521 cagcccgggg gatcccatgg tacgcgtgct agaggcatca aataaaacga aaggctcagt
     2581 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     2641 caaatccgcc gccctagacc tagggggtta tcattggggc tcactcaaag gcggtaatca
     2701 gataaaaaaa atccttagct ttcgctaagg atgatttctg ctagtattat tatttgccga
     2761 ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg
     2821 aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct
     2881 gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt
     2941 tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc
     3001 cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat
     3061 cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat
     3121 gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga
     3181 tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat
     3241 aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct
     3301 cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg
     3361 tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc
     3421 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct
     3481 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca
     3541 tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata
     3601 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
     3661 gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     3721 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat aacacccctt gtattactgt
     3781 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac
     3841 atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga
     3901 aggatcaggt tacattgtcg atctgttcat ggtgaacagc tttaaatgca ccaaaaactc
     3961 gtaaaagctc tgatgtatct atctttttta caccgttttc atctgtgcat atggacagtt
     4021 ttccctttga tatctaacgg tgaacagttg ttctactttt gtttgttagt cttgatgctt
     4081 cactgataga tacaagagcc ataagaacct cagatccttc cgtatttagc cagtatgttc
     4141 tctagtgtgg ttcgttgttt ttgcgtgagc catgagaacg aaccattgag atcatactta
     4201 ctttgcatgt cactcaaaaa ttttgcctca aaactggtga gctgaatttt tgcagttaaa
     4261 gcatcgtgta gtgtttttct tagtccgtta tgtaggtagg aatctgatgt aatggttgtt
     4321 ggtattttgt caccattcat ttttatctgg ttgttctcaa gttcggttac gagatccatt
     4381 tgtctatcta gttcaacttg gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc
     4441 accaatttca tattgctgta agtgtttaaa tctttactta ttggtttcaa aacccattgg
     4501 ttaagccttt taaactcatg gtagttattt tcaagcatta acatgaactt aaattcatca
     4561 aggctaatct ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt taataaccac
     4621 tcataaatcc tcatagagta tttgttttca aaagacttaa catgttccag attatatttt
     4681 atgaattttt ttaactggaa aagataaggc aatatctctt cactaaaaac taattctaat
     4741 ttttcgcttg agaacttggc atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa
     4801 ggattcctga tttccacagt tctcgtcatc agctctctgg ttgctttagc taatacacca
     4861 taagcatttt ccctactgat gttcatcatc tgagcgtatt ggttataagt gaacgatacc
     4921 gtccgttctt tccttgtagg gttttcaatc gtggggttga gtagtgccac acagcataaa
     4981 attagcttgg tttcatgctc cgttaagtca tagcgactaa tcgctagttc atttgctttg
     5041 aaaacaacta attcagacat acatctcaat tggtctaggt gattttaatc actataccaa
     5101 ttgagatggg ctagtcaatg ataattacta gtccttttcc tttgagttgt gggtatctgt
     5161 aaattctgct agacctttgc tggaaaactt gtaaattctg ctagaccctc tgtaaattcc
     5221 gctagacctt tgtgtgtttt ttttgtttat attcaagtgg ttataattta tagaataaag
     5281 aaagaataaa aaaagataaa aagaatagat cccagccctg tgtataa
//
