LOCUS       Exported                5038 bp ds-DNA     circular SYN 10-JAN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pROP-IP-FBFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 10, 2019 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      495..589
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(661..1371)
                     /codon_start=1
                     /note="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    1372..1404
                     /note="RBS"
     misc_feature    complement(1405..1452)
                     /note="BBa_J23107"
     terminator      1537..1566
                     /note="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     promoter        1699..1727
                     /note="BBa_J23101"
     misc_feature    1736..1751
                     /note="ROP IP"
     RBS             1762..1796
                     /note="Synthetic RBS"
     CDS             1797..2210
                     /codon_start=1
                     /note="EcFbFP"
                     /translation="MASFQSFGIPGQLEVIKKALDHVRVGVVITDPALEDNPIVYVNQG
                     FVQMTGYETEEILGKNARFLQGKHTDPAEVDNIRTALQNKEPVTVQIQNYKKDGTMFWN
                     ELNIDPMEIEDKTYFVGIQNDITKQKEYEKLLE"
     terminator      2270..2356
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2461..3252)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="SmR"
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(3821..4771)
                     /codon_start=1
                     /gene="rep101"
                     /product="protein needed for replication with the pSC101 
                     origin"
                     /note="Rep101"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4819..3
                     /note="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc
       61 tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa
      121 atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg
      181 acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc
      241 gccttttatg gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct
      301 tctcagggcg ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc
      361 agttcctgcc ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc
      421 agactggcta atgcacccag taaggcagcg gtatcatcaa caggcttacc cgtcttactg
      481 tccctagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
      541 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
      601 gtaaacttgg tctgacagct ctagctccgg caaaaaaacg ggcaaggtgt caccaccctg
      661 ttatttgtac agctcatcca tgccaccggt agaatgacga ccctccgcgc gctcatattg
      721 ctctacgatc gtataatctt cattatgaga ggtgatgtcc agtttaatat tcacattgta
      781 cgcgccaggc agctgcacag gtttcttggc tttgtacgtg gttttcactt cagcgtcata
      841 atggccgcca tctttcagtt tcaggcgctg tttaatttcg cctttcagcg caccatcttc
      901 cggatacatg cgttcgctag acgcctccca acccatcgtc tttttctgca tcaccgggcc
      961 atcagatgga aaattagtac cacgcagttt aactttatag atgaactcgc catcctgcag
     1021 ggaggagtcc tgagtgacgg tcacgacacc accatcttca aaattcatta cgcgttccca
     1081 cttgaaacct tccggaaaag acagcttcag atagtccggg atatccgctg ggtgtttaac
     1141 atacgcttta gaaccgtaca taaattgcgg gctcaggatg tcccacgcaa aaggcagcgg
     1201 gccgccttta gtcactttca gtttggcggt ctgggtgcct tcatacggac ggccctcgcc
     1261 ttcgccttcg atctcgaact cgtgaccgtt aacagaaccc tccatgtgaa ctttgaagcg
     1321 catgaactct ttaatgatag ccatgttatc ctcctcgccc ttggaaacca ttcttttcct
     1381 tcttatcctc gcttaccttc ttgctccatg ctagcataat acctagggct gagctagccg
     1441 taaaggcgcg cccccttttt ctttaaaacc gaaaagatta cttcgcgttt gccacctgac
     1501 gtctaagaaa aggaatattc agcaatttgc ccgtgccgaa gaaaggccca cccgtgaagg
     1561 tgagccagtg agttgattgc tacgtaacaa ttccgacgtc taagaaacca ttattatcat
     1621 gacattaacc tataaaaata ggcgtatcac gaggcccttt tttcgtcttc acctcgagat
     1681 gctagcaaaa aaagagtatt tacagctagc tcagtcctag gtattattac agccatgtga
     1741 aatattgagc aaaaagaatt cacaattctt ataaaatcta ttaggaggtt tatagcatgg
     1801 cgtcctttca gtcttttggt attccgggtc agctggaggt gattaagaag gcgctggatc
     1861 atgtgcgcgt tggcgtcgtt atcaccgatc cggccctgga agataacccg attgtgtatg
     1921 tgaatcaggg cttcgtccaa atgaccggct acgaaacgga agaaatctta ggcaaaaatg
     1981 cacgcttcct gcaaggcaaa cacaccgacc cggcagaggt cgataacatt cgcaccgcgc
     2041 tgcaaaataa agaaccggtt acggtccaga tccagaacta taaaaaagac ggcacgatgt
     2101 tctggaatga actgaacatt gacccgatgg agattgagga taagacgtac tttgttggta
     2161 ttcagaacga catcaccaaa cagaaggagt acgagaagct gttggagtaa aagcttgata
     2221 tcgaattcct gcagcccggg ggatcccatg gtacgcgtgc tagaggcatc aaataaaacg
     2281 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     2341 cctgagtagg acaaatccgc cgccctagac ctagggggtt atcattgggg ctcactcaaa
     2401 ggcggtaatc agataaaaaa aatccttagc tttcgctaag gatgatttct gctagtatta
     2461 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
     2521 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
     2581 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
     2641 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
     2701 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
     2761 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
     2821 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
     2881 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
     2941 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
     3001 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
     3061 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
     3121 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
     3181 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
     3241 cgcttccctc atgatgttta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3301 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     3361 aggcatagac tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
     3421 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca taacacccct
     3481 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
     3541 tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt
     3601 tgctgagttg aaggatcagg ttacattgtc gatctgttca tggtgaacag ctttaaatgc
     3661 accaaaaact cgtaaaagct ctgatgtatc tatctttttt acaccgtttt catctgtgca
     3721 tatggacagt tttccctttg atatctaacg gtgaacagtt gttctacttt tgtttgttag
     3781 tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt ccgtatttag
     3841 ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga
     3901 gatcatactt actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt
     3961 ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag gaatctgatg
     4021 taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta
     4081 cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc
     4141 gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca
     4201 aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact
     4261 taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt
     4321 ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca
     4381 gattatattt tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa
     4441 ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc
     4501 ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag
     4561 ctaatacacc ataagcattt tccctactga tgttcatcat ctgagcgtat tggttataag
     4621 tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca
     4681 cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt
     4741 catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat
     4801 cactatacca attgagatgg gctagtcaat gataattact agtccttttc ctttgagttg
     4861 tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct
     4921 ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt
     4981 atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataa
//
