LOCUS       pBA904_gBA405           8914 bp ds-DNA     circular     18-JUL-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     LTR             844..1024
                     /label="LTR 5'"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6574..6589
                     /label="cPPT (pSico annotation)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     PCR_primer      complement(3061..3076)
                     /label="BA100"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     gene            4996..5691
                     /label="BFP"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     LTR             2459..2473
                     /label="cPPT"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     LTR             6583..6597
                     /label="cPPT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    3077..3083
                     /label="polyT-track"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1686..1919
                     /label="RRE (pSico annotation)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    6911..7091
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     gene            complement(7924..8583)
                     /label="AmpR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(3200..3218)
                     /label="oBA421"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        263..826
                     /label="pU6 5'CMV promoter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     PCR_primer      complement(3086..3109)
                     /label="oBA90"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4921..4983
                     /label="t2a"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2969..2969
                     /label="A-U flip"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     LTR             5793..6393
                     /label="wPRE"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2862..2882
                     /label="oBA420"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2934..2945
                     /label="BstXI"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        8823..8851
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2965..2974
                     /label="sgRNA F + E constant region"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3053..3061
                     /label="half hairpin1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3031..3052
                     /label="X22_v2_6"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     gene            4324..4920
                     /label="PURO"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2450..2465
                     /label="cPPT (pSico annotation)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      7144..7826
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        2631..2944
                     /label="mouse U6 - modified"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    6574..6595
                     /label="U3PPT (pSico annotation)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    239..815
                     /label="CMV_immearly_promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3021..3030
                     /label="half hairpin1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        3141..4312
                     /label="ef1a"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2838..2845
                     /label="loxP 8-bp spacer region"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     LTR             6920..7100
                     /label="3' LTR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3000..3000
                     /label="A-U flip"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    6993..6998
                     /label="poly A signal"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1126..1170
                     /label="HIV-1_psi_pack"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1695..1928
                     /label="RRE"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2856..2861
                     /label="mU6 TATA box"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    835..1015
                     /label="HIV-1_5_LTR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5796..6383
                     /label="wPRE (pSico annotation)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2945..2964
                     /label="eGFP-NT2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_recomb     2825..2858
                     /label="LoxP recombination site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     PCR_primer      2674..2693
                     /label="oBA89"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
       61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
     1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc
     2641 gccatctcta ggcccgcgcc ggccccctcg cacggacttg tgggagaagc tcggctactc
     2701 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga
     2761 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat
     2821 ttctataact tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca
     2881 ccctaactgt aaagtaattg tgtgttttga gactataaGt atcccttgga gaaCCAcctT
     2941 GTTGGACCAG GATGGGCACC ACCCGTTTaA GAGCTAaGCT GGAAACAGCA TAGCAAGTTt
     3001 AAATAAGGCT AGTCCGTTAT CAACTTggcc GCTTTAAGGC CGGTCCTAGC AAggccAAGT
     3061 GGCACCGAGT CGGTGCTTTT TTTCtcgagt actaggatcc attaggcggc cgcgtggata
     3121 accgtattac cgccatgcat GTGCCCGTCA GTGGGCAGAG CGCACATCGC CCACAGTCCC
     3181 CGAGAAGTTG GGGGGAGGGG TCGGCAATTG AACCGGTGCC TAGAGAAGGT GGCGCGGGGT
     3241 AAACTGGGAA AGTGATGTCG TGTACTGGCT CCGCCTTTTT CCCGAGGGTG GGGGAGAACC
     3301 GTATATAAGT GCAGTAGTCG CCGTGAACGT TCTTTTTCGC AACGGGTTTG CCGCCAGAAC
     3361 ACAGGTAAGT GCCGTGTGTG GTTCCCGCGG GCCTGGCCTC TTTACGGGTT ATGGCCCTTG
     3421 CGTGCCTTGA ATTACTTCCA CCTGGCTGCA GTACGTGATT CTTGATCCCG AGCTTCGGGT
     3481 TGGAAGTGGG TGGGAGAGTT CGAGGCCTTG CGCTTAAGGA GCCCCTTCGC CTCGTGCTTG
     3541 AGTTGAGGCC TGGCCTGGGC GCTGGGGCCG CCGCGTGCGA ATCTGGTGGC ACCTTCGCGC
     3601 CTGTCTCGCT GCTTTCGATA AGTCTCTAGC CATTTAAAAT TTTTGATGAC CTGCTGCGAC
     3661 GCTTTTTTTC TGGCAAGATA GTCTTGTAAA TGCGGGCCAA GATCTGCACA CTGGTATTTC
     3721 GGTTTTTGGG GCCGCGGGCG GCGACGGGGC CCGTGCGTCC CAGCGCACAT GTTCGGCGAG
     3781 GCGGGGCCTG CGAGCGCGGC CACCGAGAAT CGGACGGGGG TAGTCTCAAG CTGGCCGGCC
     3841 TGCTCTGGTG CCTGGCCTCG CGCCGCCGTG TATCGCCCCG CCCTGGGCGG CAAGGCTGGC
     3901 CCGGTCGGCA CCAGTTGCGT GAGCGGAAAG ATGGCCGCTT CCCGGCCCTG CTGCAGGGAG
     3961 CTCAAAATGG AGGACGCGGC GCTCGGGAGA GCGGGCGGGT GAGTCACCCA CACAAAGGAA
     4021 AAGGGCCTTT CCGTCCTCAG CCGTCGCTTC ATGTGACTCC ACGGAGTACC GGGCGCCGTC
     4081 CAGGCACCTC GATTAGTTCT CGcGCTTTTG GAGTACGTCG TCTTTAGGTT GGGGGGAGGG
     4141 GTTTTATGCG ATGGAGTTTC CCCACACTGA GTGGGTGGAG ACTGAAGTTA GGCCAGCTTG
     4201 GCACTTGATG TAATTCTCCT TGGAATTTGC CCTTTTTGAG TTTGGATCTT GGTTCATTCT
     4261 CAAGCCTCAG ACAGTGGTTC AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAgCTAGCCC
     4321 ACCATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCCGGGCC
     4381 GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGACCCG
     4441 GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC
     4501 GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG
     4561 GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC
     4621 GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG
     4681 GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG
     4741 GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC
     4801 CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC
     4861 GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC
     4921 GGATCGGGAG AGGGCAGAGG AAGTCTGCTA ACATGCGGTG ACGTCGAGGA GAATCCTGGC
     4981 CCAccggtcg ccaccAGCGA GCTGATTAAG GAGAACATGC ACATGAAGCT GTACATGGAG
     5041 GGCACCGTGG ACAACCATCA CTTCAAGTGC ACATCCGAGG GCGAAGGCAA GCCCTACGAG
     5101 GGCACCCAGA CCATGAGAAT CAAGGTGGTC GAGGGCGGCC CTCTCCCCTT CGCCTTCGAC
     5161 ATCCTGGCTA CTAGCTTCCT CTACGGCAGC AAGACCTTCA TCAACCACAC CCAGGGCATC
     5221 CCCGACTTCT TCAAGCAGTC CTTCCCTGAG GGCTTCACAT GGGAGAGAGT CACCACATAC
     5281 GAAGACGGGG GCGTGCTGAC CGCTACCCAG GACACCAGCC TCCAGGACGG CTGCCTCATC
     5341 TACAACGTCA AGATCAGAGG GGTGAACTTC ACATCCAACG GCCCTGTGAT GCAGAAGAAA
     5401 ACACTCGGCT GGGAGGCCTT CACCGAGACG CTGTACCCCG CTGACGGCGG CCTGGAAGGC
     5461 AGAAACGACA TGGCCCTGAA GCTCGTGGGC GGGAGCCATC TGATCGCAAA CATCAAGACC
     5521 ACATATAGAT CCAAGAAACC CGCTAAGAAC CTCAAGATGC CTGGCGTCTA CTATGTGGAC
     5581 TACAGACTGG AAAGAATCAA GGAGGCCAAC AACGAGACCT ACGTCGAGCA GCACGAGGTG
     5641 GCAGTGGCCA GATACTGCGA CCTCCCTAGC AAACTGGGGC ACAAGCTTAA Ttaagaattc
     5701 gtcgagggac ctaataactt cgtatagcat acattatacg aagttataca tgtttaaggg
     5761 ttccggttcc actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac
     5821 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     5881 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5941 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6001 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6061 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6121 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6181 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     6241 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6301 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6361 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct
     6421 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     6481 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     6541 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     6601 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     6661 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     6721 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     6781 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     6841 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     6901 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6961 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7021 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7081 agtgtggaaa atctctagca gcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7141 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7201 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7261 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7321 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7381 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7441 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7501 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7561 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7621 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7681 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7741 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7801 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7861 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7921 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7981 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8041 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8101 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8161 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8221 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8281 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8341 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8401 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8461 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8521 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8581 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8641 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8701 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8761 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8821 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8881 tccgcgcaca tttccccgaa aagtgccacc tgac
//