LOCUS       Exported                4502 bp ds-DNA     circular SYN 17-JAN-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pCS2-aBTX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene Viewer 3.0.3
            http://www.snapgene.com
COMMENT     >pCS2+
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6"
     misc_feature    106..153
                     /label=secretion sequence from fish trypsin
                     /note="secretion sequence from fish trypsin"
     gene            154..180
                     /label=FLAG epitope tag
                     /note="FLAG epitope tag"
     misc_recomb     181..399
                     /label=a-Bgtx from B. multicinctus (what Ines used in zeb
                     /note="a-Bgtx from B. multicinctus (what Ines used in zeb"
                     /note="a-Bgtx from B. multicinctus (what Ines used in 
                     zebrafish)"
     primer_bind     400..429
                     /label=linker
                     /note="linker"
     repeat_region   430..510
                     /label=C-terminal GPI anchor  from fish lye
                     /note="C-terminal GPI anchor from fish lye"
                     /note="C-terminal GPI anchor  from fish lye"
     polyA_signal    555..746
                     /label=SV40 late polyA
                     /note="SV40 late polyA"
     primer_bind     complement(796..815)
                     /label=T3
                     /note="T3"
     primer_bind     complement(833..853)
                     /label=M13-rev
                     /note="M13-rev"
     misc_binding    complement(859..881)
                     /label=LacO
                     /note="LacO"
     rep_origin      complement(1221..1903)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ColE1 origin"
     CDS             complement(2001..2660)
                     /label=AmpR
                     /note="AmpR"
     rep_origin      complement(3121..3427)
                     /direction=LEFT
                     /label=F1 ori
                     /note="F1 ori"
     CDS             complement(3446..3514)
                     /label=LacZ alpha
                     /note="LacZ alpha"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg ccaccatgaa agcatttata
      121 ctgttagctc tattcgctgt ggcatacgct gctgactata aggacgatga tgataagctt
      181 attgtctgtc acacaacagc taccagccca atttccgcag tcacttgccc acccggagag
      241 aacctctgct ataggaagat gtggtgtgac gctttctgta gtagcagagg caaggtcgta
      301 gagttggggt gcgctgccac ttgtccttca aaaaagcctt atgaggaggt gacctgctgc
      361 agtaccgaca agtgtaaccc tcatccaaag cagcgaccag gagctgctgc cggaggcgcc
      421 ttgtgtaacg gtgctagcgc cgtgcaactc agtacaaccg ccgctttctc tactgcttta
      481 ttggcaagta tatggtcaag ctacatgttg tgactcgagc ctctagaact atagtgagtc
      541 gtattacgta gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
      601 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
      661 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
      721 tcagggggag gtgtgggagg ttttttaatt cgcggccgcg gcgccaatgc attgggcccg
      781 gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
      841 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      901 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
      961 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1021 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1081 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1141 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1201 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1261 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1321 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1381 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1441 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1501 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1561 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1621 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     1681 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1741 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1801 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1861 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1921 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1981 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2041 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2101 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2161 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2221 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2281 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2341 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2401 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2461 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2521 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2581 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2641 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2701 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2761 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2821 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2881 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2941 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     3001 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3061 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3121 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     3181 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     3241 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     3301 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     3361 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     3421 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     3481 ggcgatcggt gcgggcctct tcgctattac gccagtcgac catagccaat tcaatatggc
     3541 gtatatggac tcatgccaat tcaatatggt ggatctggac ctgtgccaat tcaatatggc
     3601 gtatatggac tcgtgccaat tcaatatggt ggatctggac cccagccaat tcaatatggc
     3661 ggacttggca ccatgccaat tcaatatggc ggacttggca ctgtgccaac tggggagggg
     3721 tctacttggc acggtgccaa gtttgaggag gggtcttggc cctgtgccaa gtccgccata
     3781 ttgaattggc atggtgccaa taatggcggc catattggct atatgccagg atcaatatat
     3841 aggcaatatc caatatggcc ctatgccaat atggctattg gccaggttca atactatgta
     3901 ttggccctat gccatatagt attccatata tgggttttcc tattgacgta gatagcccct
     3961 cccaatgggc ggtcccatat accatatatg gggcttccta ataccgccca tagccactcc
     4021 cccattgacg tcaatggtct ctatatatgg tctttcctat tgacgtcata tgggcggtcc
     4081 tattgacgta tatggcgcct cccccattga cgtcaattac ggtaaatggc ccgcctggct
     4141 caatgcccat tgacgtcaat aggaccaccc accattgacg tcaatgggat ggctcattgc
     4201 ccattcatat ccgttctcac gccccctatt gacgtcaatg acggtaaatg gcccacttgg
     4261 cagtacatca atatctatta atagtaactt ggcaagtaca ttactattgg aaggacgcca
     4321 gggtacattg gcagtactcc cattgacgtc aatggcggta aatggcccgc gatggctgcc
     4381 aagtacatcc ccattgacgt caatggggag gggcaatgac gcaaatgggc gttccattga
     4441 cgtaaatggg cggtaggcgt gcctaatggg aggtctatat aagcaatgct cgtttaggga
     4501 ac
//
