LOCUS       pdCas9gabdp2-GFP101     7264 bp ds-DNA     circular     15-NOV-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    291..325
                     /label="BBa_J23101"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     gene            353..1069
                     /label="GFP"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     RBS             2540..2551
                     /label="BBa_B0034"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     RBS             328..352
                     /label="BBa_B0034"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2234..2441)
                     /label="TetR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      6673..6808
                     /label="BBa-B0015 Terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2442..2522
                     /label="gabDP2"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    join(6814..7264,1..263)
                     /label="p15A ori"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3358..3382
                     /label="dCas9 seq 1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2558..4255
                     /label="dCas9"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     gene            complement(1428..2087)
                     /label="CAT/CamR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1091..1226
                     /label="BBa_B0015"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             4256..6664
                     /label="dCas9"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt
       61 tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg
      121 ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac
      181 cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat
      241 ctcaagaaga tcatcttatt aatcagataa aatatttcta gatttcagtg TTTAcagcta
      301 gctcagtcct aggtattATG CTAGCtgtct agagaaagag gagaaatact agatgcgtaa
      361 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      421 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
      481 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
      541 tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt
      601 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
      661 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
      721 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
      781 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
      841 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
      901 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
      961 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
     1021 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat aaaggatcca
     1081 aactcgagta aggatctcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1141 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1201 ttcgggtggg cctttctgcg tttataccta gggatatatt agtgcaattt atctcttcaa
     1261 atgtagcacc tgaagtcagc cccatacgat ataagttgtt actagtgctt ggattctcac
     1321 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     1381 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     1441 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     1501 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     1561 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     1621 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
     1681 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     1741 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     1801 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     1861 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     1921 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     1981 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     2041 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     2101 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     2161 tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccagat atcgacgtct
     2221 taagacccac tttcacattt aagttgtttt tctaatccgc atatgatcaa ttcaaggccg
     2281 aataagaagg ctggctctgc accttggtga tcaaataatt cgatagcttg tcgtaataat
     2341 ggcggcatac tatcagtagt aggtgtttcc ctttcttctt tagcgacttg atgctcttga
     2401 tcttccaata cgcaacctaa agtaaaatgc cccacagcgc tgagattttg ggctcgtcgg
     2461 ggattcgccg ggtgctgcaa aaccatctac gctcaggact gggcgagatg aaaaactcgc
     2521 tgaagaattc aaaagatcta aagaggagaa aggatctatg gataagaaat actcaatagg
     2581 cttagctatc ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc
     2641 gtctaaaaag ttcaaggttc tgggaaatac agaccgccac agtatcaaaa AAAATCTTAT
     2701 AGGGGCTCTT TTATTTGACA GTGGAGAGAC AGCGGAAGCG ACTCGTCTCA AACGGACAGC
     2761 TCGTAGAAGG TATACACGTC GGAAGAATCG TATTTGTTAT CTACAGGAGA TTTTTTCAAA
     2821 TGAGATGGCG AAAGTAGATG ATAGTTTCTT TCATCGACTT GAAGAGTCTT TTTTGGTGGA
     2881 AGAAGACAAG AAGCATGAAC GTCATCCTAT TTTTGGAAAT ATAGTAGATG AAGTTGCTTA
     2941 TCATGAGAAA TATCCAACTA TCTATCATCT GCGAAAAAAA TTGGTAGATT CTACTGATAA
     3001 AGCGGATTTG CGCTTAATCT ATTTGGCCTT AGCGCATATG ATTAAGTTTC GTGGTCATTT
     3061 TTTGATTGAG GGAGATTTAA ATCCTGATAA TAGTGATGTG GACAAACTAT TTATCCAGTT
     3121 GGTACAAACC TACAATCAAT TATTTGAAGA AAACCCTATT AACGCAAGTG GAGTAGATGC
     3181 TAAAGCGATT CTTTCTGCAC GATTGAGTAA ATCAAGACGA TTAGAAAATC TCATTGCTCA
     3241 GCTCCCCGGT GAGAAGAAAA ATGGCTTATT TGGGAATCTC ATTGCTTTGT CATTGGGTTT
     3301 GACCCCTAAT TTTAAATCAA ATTTTGATTT GGCAGAAGAT GCTAAATTAC AGCTTTCAAA
     3361 AGATACTTAC GATGATGATT TAGATAATTT ATTGGCGCAA ATTGGAGATC AATATGCTGA
     3421 TTTGTTTTTG GCAGCTAAGA ATTTATCAGA TGCTATTTTA CTTTCAGATA TCCTAAGAGT
     3481 AAATACTGAA ATAACTAAGG CTCCCCTATC AGCTTCAATG ATTAAACGCT ACGATGAACA
     3541 TCATCAAGAC TTGACTCTTT TAAAAGCTTT AGTTCGACAA CAACTTCCAG AAAAGTATAA
     3601 AGAAATCTTT TTTGATCAAT CAAAAAACGG ATATGCAGGT TATATTGATG GGGGAGCTAG
     3661 CCAAGAAGAA TTTTATAAAT TTATCAAACC AATTTTAGAA AAAATGGATG GTACTGAGGA
     3721 ATTATTGGTG AAACTAAATC GTGAAGATTT GCTGCGCAAG CAACGGACCT TTGACAACGG
     3781 CTCTATTCCC CATCAAATTC ACTTGGGTGA GCTGCATGCT ATTTTGAGAA GACAAGAAGA
     3841 CTTTTATCCA TTTTTAAAAG ACAATCGTGA GAAGATTGAA AAAATCTTGA CTTTTCGAAT
     3901 TCCTTATTAT GTTGGTCCAT TGGCGCGTGG CAATAGTCGT TTTGCATGGA TGACTCGGAA
     3961 GTCTGAAGAA ACAATTACCC CATGGAATTT TGAAGAAGTT GTCGATAAAG GTGCTTCAGC
     4021 TCAATCATTT ATTGAACGCA TGACAAACTT TGATAAAAAT CTTCCAAATG AAAAAGTACT
     4081 ACCAAAACAT AGTTTGCTTT ATGAGTATTT TACGGTTTAT AACGAATTGA CAAAGGTCAA
     4141 ATATGTTACT GAAGGAATGC GAAAACCAGC ATTTCTTTCA GGTGAACAGA AGAAAGCCAT
     4201 TGTTGATTTA CTCTTCAAAA CAAATCGAAA AGTAACCGTT AAGCAATTAA AAGAAGATTA
     4261 TTTCAAAAAA ATAGAATGTT TTGATAGTGT TGAAATTTCA GGAGTTGAAG ATAGATTTAA
     4321 TGCTTCATTA GGTACCTACC ATGATTTGCT AAAAATTATT AAAGATAAAG ATTTTTTGGA
     4381 TAATGAAGAA AATGAAGATA TCTTAGAGGA TATTGTTTTA ACATTGACCT TATTTGAAGA
     4441 TAGGGAGATG ATTGAGGAAA GACTTAAAAC ATATGCTCAC CTCTTTGATG ATAAGGTGAT
     4501 GAAACAGCTT AAACGTCGCC GTTATACTGG TTGGGGACGT TTGTCTCGAA AATTGATTAA
     4561 TGGTATTAGG GATAAGCAAT CTGGCAAAAC AATATTAGAT TTTTTGAAAT CAGATGGTTT
     4621 TGCCAATCGC AATTTTATGC AGCTGATCCA TGATGATAGT TTGACATTTA AAGAAGACAT
     4681 TCAAAAAGCA CAAGTGTCTG GACAAGGCGA TAGTTTACAT GAACATATTG CAAATTTAGC
     4741 TGGTAGCCCT GCTATTAAAA AAGGTATTTT ACAGACTGTA AAAGTTGTTG ATGAATTGGT
     4801 CAAAGTAATG GGGCGGCATA AGCCAGAAAA TATCGTTATT GAAATGGCAC GTGAAAATCA
     4861 GACAACTCAA AAGGGCCAGA AAAATTCGCG AGAGCGTATG AAACGAATCG AAGAAGGTAT
     4921 CAAAGAATTA GGAAGTCAGA TTCTTAAAGA GCATCCTGTT GAAAATACTC AATTGCAAAA
     4981 TGAAAAGCTC TATCTCTATT ATCTCCAAAA TGGAAGAGAC ATGTATGTGG ACCAAGAATT
     5041 AGATATTAAT CGTTTAAGTG ATTATGATGT CGATGCCATT GTTCCACAAA GTTTCCTTAA
     5101 AGACGATTCA ATAGACAATA AGGTCTTAAC GCGTTCTGAT AAAAATCGTG GTAAATCGGA
     5161 TAACGTTCCA AGTGAAGAAG TAGTCAAAAA GATGAAAAAC TATTGGAGAC AACTTCTAAA
     5221 CGCCAAGTTA ATCACTCAAC GTAAGTTTGA TAATTTAACG AAAGCTGAAC GTGGAGGTTT
     5281 GAGTGAACTT GATAAAGCTG GTTTTATCAA ACGCCAATTG GTTGAAACTC GCCAAATCAC
     5341 TAAGCATGTG GCACAAATTT TGGATAGTCG CATGAATACT AAATACGATG AAAATGATAA
     5401 ACTTATTCGA GAGGTTAAAG TGATTACCTT AAAATCTAAA TTAGTTTCTG ACTTCCGAAA
     5461 AGATTTCCAA TTCTATAAAG TACGTGAGAT TAACAATTAC CATCATGCCC ATGATGCGTA
     5521 TCTAAATGCC GTCGTTGGAA CTGCTTTGAT TAAGAAATAT CCAAAACTTG AATCGGAGTT
     5581 TGTCTATGGT GATTATAAAG TTTATGATGT TCGTAAAATG ATTGCTAAGT CTGAGCAAGA
     5641 AATAGGCAAA GCAACCGCAA AATATTTCTT TTACTCTAAT ATCATGAACT TCTTCAAAAC
     5701 AGAAATTACA CTTGCAAATG GAGAGATTCG CAAACGCCCT CTAATCGAAA CTAATGGGGA
     5761 AACTGGAGAA ATTGTCTGGG ATAAAGGGCG AGATTTTGCC ACAGTGCGCA AAGTATTGTC
     5821 CATGCCCCAA GTCAATATTG TCAAGAAAAC AGAAGTACAG ACAGGCGGAT TCTCCAAGGA
     5881 GTCAATTTTA CCAAAAAGAA ATTCGGACAA GCTTATTGCT CGTAAAAAAG ACTGGGATCC
     5941 AAAAAAATAT GGTGGTTTTG ATAGTCCAAC GGTAGCTTAT TCAGTCCTAG TGGTTGCTAA
     6001 GGTGGAAAAA GGGAAATCGA AGAAGTTAAA ATCCGTTAAA GAGTTACTAG GGATCACAAT
     6061 TATGGAAAGA AGTTCCTTTG AAAAAAATCC GATTGACTTT TTAGAAGCTA AAGGATATAA
     6121 GGAAGTTAAA AAAGACTTAA TCATTAAACT ACCTAAATAT AGTCTTTTTG AGTTAGAAAA
     6181 CGGTCGTAAA CGGATGCTGG CTAGTGCCGG AGAATTACAA AAAGGAAATG AGCTGGCTCT
     6241 GCCAAGCAAA TATGTGAATT TTTTATATTT AGCTAGTCAT TATGAAAAGT TGAAGGGTAG
     6301 TCCAGAAGAT AACGAACAAA AACAATTGTT TGTGGAGCAG CATAAGCATT ATTTAGATGA
     6361 GATTATTGAG CAAATCAGTG AATTTTCTAA GCGTGTTATT TTAGCAGATG CCAATTTAGA
     6421 TAAAGTTCTT AGTGCATATA ACAAACATAG AGACAAACCA ATACGTGAAC AAGCAGAAAA
     6481 TATTATTCAT TTATTTACGT TGACGAATCT TGGAGCTCCC GCTGCTTTTA AATATTTTGA
     6541 TACAACAATT GATCGTAAAC GATATACGTC TACAAAAGAA GTTTTAGATG CCACTCTTAT
     6601 CCATCAATCC ATCACTGGTC TTTATGAAAC ACGCATTGAT TTGAGTCAGC TAGGAGGTGA
     6661 CTAACtcgag taaggatctc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     6721 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     6781 ccttcgggtg ggcctttctg cgtttatacc tagggatata ttccgcttcc tcgctcactg
     6841 actcgctacg ctcggtcgtt cgactgcggc gagcggaaat ggcttacgaa cggggcggag
     6901 atttcctgga agatgccagg aagatactta acagggaagt gagagggccg cggcaaagcc
     6961 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
     7021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggcg gctccctcgt
     7081 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
     7141 gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
     7201 tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7261 ccaa
//