LOCUS       PLC_RFP                 5877 bp ds-DNA     circular     12-MAR-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     RBS             complement(4232..4243)
                     /label="BBa_B0034"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     terminator      5080..5215
                     /label="BBa-B0015 Terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4347..4364
                     /label="EL222 binding region"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        4294..4346
                     /label="pLasI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     RBS             2447..2466
                     /label="Default RBS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     Gene            complement(3504..4223)
                     /label="LasR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      3162..3297
                     /label="BBa-B0015 Terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(join(5349..5877,1..154))
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     Terminator      complement(3363..3477)
                     /label="BBa_B0015"
                     /ApEinfo_revcolor=#7ea7d8
                     /ApEinfo_fwdcolor=#7ea7d8
     Promoter        complement(4244..4278)
                     /label="J23108"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     RBS             4365..4384
                     /label="RFP-RBS"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     gene            complement(1228..2106)
                     /label="AraC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     RBS             4365..4384
                     /label="Default RBS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2380..2409
                     /label="pBRBAD"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     gene            4385..5062
                     /label="RFP"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     gene            2467..3144
                     /label="RFP"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(291..1082)
                     /label="Kan/neoR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
       61 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
      121 aacgaaaact cacgttaagg gattttggtc atgactagtg cttggattct caccaataaa
      181 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
      241 tggatctatc aacaggagtc caagcgagct ctcgaacccc agagtcccgc tcagaagaac
      301 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
      361 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
      421 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
      481 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
      541 tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
      601 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
      661 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
      721 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
      781 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
      841 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
      901 tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
      961 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     1021 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     1081 atcatgcgaa acgatcctca tcctgtctct tgatcagatc atgatcccct gcgccatcag
     1141 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
     1201 ggcgccccag ctggcaattc cgacgtctta tgacaacttg acggctacat cattcacttt
     1261 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg
     1321 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg
     1381 ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac
     1441 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg
     1501 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca
     1561 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg
     1621 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc
     1681 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc
     1741 cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta
     1801 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc
     1861 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc
     1921 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt
     1981 cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa
     2041 acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc
     2101 agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag
     2161 acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct
     2221 tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag
     2281 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta
     2341 tgccatagca tttttatcca taagattagc ggattctacc tgacgGGTAG CCTTTAGTCC
     2401 ATGtactgtt tctccatacc cgtttttttg ggaattcaaa agatctttta agaaggagat
     2461 atacatatgg cgagtagcga agacgttatc aaagagttca tgcgtttcaa agttcgtatg
     2521 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac
     2581 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg
     2641 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac
     2701 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac
     2761 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc
     2821 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa
     2881 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa
     2941 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa
     3001 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa
     3061 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa
     3121 ggtcgtcact ccaccggtgc ttaaggatcc aaactcgagt aaggatctcc aggcatcaaa
     3181 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     3241 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatacct
     3301 agggcgttcg gctgcggcga gcggtatcag ctcactcaaT TTTTTGGGAA TTCAAGAGAT
     3361 CTTATAAACG CAGAAAGGCC CACCCGAAGG TGACCCAGTG TGACTCTAGT AGAGAGCGTT
     3421 CACCGACAAA CAACAGATAA AACGAAAGGC CCAGTCTTTC GACTGAGCCT TTCGTTTTAA
     3481 TTTGATGCCT GGCTCTAGTA TTATTAGAGA GTAATAAGAC CCAAATTAAC GGCCATAATG
     3541 GCCGCTACGC GGCGGGAGGT CACACCGAAC TTCCGCCGAA TATTTCCCAT ATGGAAGTTC
     3601 ACATTGGCTT CCGAGCAGTT GCAGATAACC GATATCTCCC AACTGGTCTT GCCGATGGCG
     3661 CACCACTGCA ACACTTCCTT CTCCCGGCTG GTCAGAACCA CCGGTTTGCT GACCGGATGT
     3721 TCGAAGGCCA GTCCGGCACC GCTTTGCAGT GCGTAGTCCT TGAGCATCCA CAGGGTCGGC
     3781 AGGACCGACT CTATGAAACG GTTGGCCTCG GCCCGGTTTT CCGCTTCCAC GCTGAGGCTC
     3841 AGCGCGCCGA GTTCGCCGCG AGCACCATGC AGCGGCATGG TCAGCCCATA CACCAGGCCG
     3901 GCGGCCGAGG CTTCCTCGAA GAACTCGTGC TGCTTTCGCG TCTGGTAGAT GGACGGTTCC
     3961 CAGAAAATCG GCAGTACGCT CTGGGTACAG TGACTGACCG TCGGGTCGAC CCGCGCGTAG
     4021 CCAGCCCGGT CGTAATGCTC GCGCCAGGCG GCCGGGTAGT TGCCGACGAT GAAGGCGTTC
     4081 TCGTAGTCCT GGCTGTCCTT AGGCAACAGG CCGAACAGGA TCTTCGAGAA TCCAAGGTCG
     4141 CTCGCCATCT TCTGGAGGAT GGCGCTCCAC TCCAATTTTC CACTTGAGCG TTCCAGCTCA
     4201 AGAAAACCGT CAACCAAGGC CATCTCTAGT ATTTCTCCTC TTTGCTAGCA TTATACCTAG
     4261 GACTGAGCTA GCTGTCAGGG ATCTGGTTCA CCGAAATCTA TCTCATTTGC TAGTTATAAA
     4321 ATTATGAAAT TTGCATAAAT TCTTCAGGTA GCCTTTAGTC CATGTTTAAG AAGGAGATAT
     4381 ACATatggcg agtagcgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga
     4441 aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga
     4501 aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga
     4561 catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat
     4621 cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt
     4681 cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat
     4741 ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa
     4801 aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg
     4861 tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac
     4921 cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact
     4981 ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg
     5041 tcgtcactcc accggtgctt aaggatccaa actcgagtaa ggatctccag gcatcaaata
     5101 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     5161 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatacctag
     5221 ggcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5281 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5341 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5401 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5461 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5521 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5581 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5641 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5701 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5761 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     5821 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatcc
//