LOCUS       Exported                6483 bp ds-DNA     circular SYN 15-NOV-2016
DEFINITION  pCAGIG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 18, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1735..2058
                     /organism="Mus musculus"
                     /chromosome="17"
                     /map="17 14.5 cM"
                     /strain="C57BL/6"
                     /mol_type="mRNA"
                     /label=source:Mus musculus
                     /db_xref="taxon:10090"
     misc_feature    84..371
                     /label=CAG_enhancer
     CDS             255..734
                     /codon_start=1
                     /label=ORF frame 3
                     /translation="MPSTPPIDVNDGKWPAWHYAQYMTLWDFPTWQYIYVLVIAITMGR
                     GEPHVLLHSPHLPPLPTPNFVFIYFLIILCSDGGGGGGGARQAGRGGARGGAGRGGEVR
                     RQPIRAARSESFLLWRGGGGGGPIKSEARGGRESLRCLRPVPRSAPPRAARPGSD"
     CDS             complement(802..1533)
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MEKGTAARRRTKALPAHFLPAGAAPLRPRPLEGVRRRLPDFGSAR
                     FGTKEVPAPSRTITIKGNGCGSPRLDSRRRSGAAAPLPRALPGPRRPRPARHPHLPPPP
                     ARHGEPRATPRTEPRTRSRAVLSNSGRGVQGGVTARPPRPHPLLTPPRTHPARSLCSPR
                     SPPAPRGTAPGRAPLAHTRSAQSPAPRPQRSQPPGSAGRTRRSPRTHTRTHPPSRSPPH
                     KGPSRSPLRLSRSHRKETSRH"
     CDS             complement(843..1346)
                     /codon_start=1
                     /label=ORF frame 2
                     /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPGT
                     ASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAAPP
                     HRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRTKGP
                     PGAL"
     gene            1735..2058
                     /gene="Hmga1"
                     /gene_synonym="AL023995; Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy"
                     /label=Hmga1
                     /note="high mobility group AT-hook 1"
                     /db_xref="GeneID:15361"
                     /db_xref="MGI:96160"
     STS             1735..2058
                     /gene="Hmga1"
                     /gene_synonym="AL023995; Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy"
                     /standard_name="PMC109291P1"
                     /label=Hmga1
                     /label=Hmga1(2)
                     /db_xref="UniSTS:270161"
     CDS             1735..2058
                     /codon_start=1
                     /gene="Hmga1"
                     /gene_synonym="AL023995; Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy"
                     /product="high mobility group protein HMG-I/HMG-Y isoform 
                     a"
                     /label=high mobility group protein HMG-I/HMG-Y isoform a
                     /note="isoform a is encoded by transcript variant 2; high 
                     mobility group protein HMG-I/HMG-Y; high mobility group 
                     protein A1; high mobility group AT-hook protein 1; high 
                     mobility group protein I"
                     /db_xref="GI:70909308"
                     /db_xref="CCDS:CCDS28564.1"
                     /db_xref="GeneID:15361"
                     /db_xref="MGI:96160"
                     /protein_id="NP_001020598.1"
                     /translation="MSESGSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQ
                     KEPSEVPTPKRPRGRPKGSKNKGAAKTRKVTTAPGRKPRGRPKKLEKEEEEGISQESSE
                     EEQ"
     exon            1735..1869
                     /gene="Hmga1"
                     /gene_synonym="AL023995; Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy"
                     /label=Hmga1
                     /label=Hmga1(1)
                     /inference="alignment:same species:1.39.8"
     misc_feature    1738..1740
                     /gene="Hmga1"
                     /gene_synonym="AL023995; Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy"
                     /label=Hmga1
                     /label=Hmga1(3)
                     /note="N-acetylserine, in isoform HMG-Y; propagated from 
                     UniProtKB/Swiss-Prot (P17095.4); acetylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    1864..1866
                     /gene="Hmga1"
                     /gene_synonym="AL023995; Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy"
                     /label=Hmga1
                     /label=Hmga1(4)
                     /note="Phosphoserine; propagated from UniProtKB/Swiss-Prot 
                     (P17095.4); phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            1870..1953
                     /gene="Hmga1"
                     /gene_synonym="AL023995; Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy"
                     /label=Hmga1
                     /label=Hmga1(11)
                     /inference="alignment:same species:1.39.8"
     misc_feature    1879..1881
                     /gene="Hmga1"
                     /gene_synonym="AL023995; Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy"
                     /label=Hmga1
                     /label=Hmga1(5)
                     /note="Phosphoserine, in isoform HMG-I; propagated from 
                     UniProtKB/Swiss-Prot (P17095.4); phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    1891..1965
                     /gene="Hmga1"
                     /gene_synonym="AL023995; Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy"
                     /label=Hmga1
                     /label=Hmga1(6)
                     /note="propagated from UniProtKB/Swiss-Prot (P17095.4); 
                     Region: Interaction with HIPK2"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    1891..1893
                     /gene="Hmga1"
                     /gene_synonym="AL023995; Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy"
                     /label=Hmga1
                     /label=Hmga1(7)
                     /note="Phosphothreonine, by HIPK2 and CDC2, in isoform 
                     HMG-I and isoform HMG-Y; propagated from 
                     UniProtKB/Swiss-Prot (P17095.4); phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     exon            1954..2004
                     /gene="Hmga1"
                     /gene_synonym="AL023995; Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy"
                     /label=Hmga1
                     /label=Hmga1(12)
                     /inference="alignment:same species:1.39.8"
     exon            2005..2058
                     /gene="Hmga1"
                     /gene_synonym="AL023995; Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy"
                     /label=Hmga1
                     /label=Hmga1(13)
                     /inference="alignment:same species:1.39.8"
     misc_feature    2029..2031
                     /gene="Hmga1"
                     /gene_synonym="AL023995; Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy"
                     /label=Hmga1
                     /label=Hmga1(8)
                     /note="Phosphoserine, in isoform HMG-Y; propagated from 
                     UniProtKB/Swiss-Prot (P17095.4); phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    2038..2040
                     /gene="Hmga1"
                     /gene_synonym="AL023995; Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy"
                     /label=Hmga1
                     /label=Hmga1(9)
                     /note="Phosphoserine, in isoform HMG-Y; propagated from 
                     UniProtKB/Swiss-Prot (P17095.4); phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    2041..2043
                     /gene="Hmga1"
                     /gene_synonym="AL023995; Hmga1a; Hmga1b; Hmgi; Hmgiy; Hmgy"
                     /label=Hmga1
                     /label=Hmga1(10)
                     /note="Phosphoserine, in isoform HMG-Y; propagated from 
                     UniProtKB/Swiss-Prot (P17095.4); phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    2126..2698
                     /label=IRES
                     /note="/translation=MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCR
                     HPPPG 
                     TASPAPRPARSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA 
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT 
                     KGPPGAL*"
     CDS             2690..3421
                     /codon_start=1
                     /label=ORF frame 2
                     /label=ORF frame 2(1)
                     /translation="MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG
                     KLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQK
                     NGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     gene            2705..3418
                     /gene="EGFP"
                     /label=EGFP
                     /note="/translation=MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATY 
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT 
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD 
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE 
                     KRDHMVLLEFVTAAGITLGMDELYK*"
     misc_feature    complement(2747..2768)
                     /label=EGFP_N_primer
                     /note="/translation=MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATY 
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT 
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD 
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE 
                     KRDHMVLLEFVTAAGITLGMDELYK*"
     misc_feature    2885..2914
                     /label=Y66 (EGFP)
                     /note="/translation=MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATY 
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT 
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD 
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE 
                     KRDHMVLLEFVTAAGITLGMDELYK*"
     misc_feature    3355..3376
                     /label=EGFP_C_primer
                     /note="/translation=MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATY 
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT 
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD 
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE 
                     KRDHMVLLEFVTAAGITLGMDELYK*"
     terminator      3475..3940
                     /label=rb_glob_PA_terminator
                     /note="/translation=MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATY 
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT 
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD 
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE 
                     KRDHMVLLEFVTAAGITLGMDELYK*"
     promoter        complement(3982..4000)
                     /label=M13_reverse_primer
                     /note="/translation=MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATY 
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT 
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD 
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE 
                     KRDHMVLLEFVTAAGITLGMDELYK*"
     misc_feature    complement(3999..4021)
                     /label=M13_pUC_rev_primer
                     /note="/translation=MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATY 
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT 
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD 
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE 
                     KRDHMVLLEFVTAAGITLGMDELYK*"
     promoter        complement(4035..4064)
                     /label=lac_promoter
                     /note="/translation=MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATY 
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT 
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD 
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE 
                     KRDHMVLLEFVTAAGITLGMDELYK*"
     misc_feature    4153..4158
                     /label=(Kishi does not know the correct sequence aroun
                     /label=(Kishi does not know the correct sequence around he
     promoter        4170..4349
                     /label=SV40_promoter
                     /note="/translation=MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATY 
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT 
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD 
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE 
                     KRDHMVLLEFVTAAGITLGMDELYK*"
     rep_origin      4205..4282
                     /label=SV40_origin
                     /note="/translation=MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATY 
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT 
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD 
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE 
                     KRDHMVLLEFVTAAGITLGMDELYK*"
     misc_feature    4267..4286
                     /label=SV40pro_F_primer
                     /note="/translation=MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATY 
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT 
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD 
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE 
                     KRDHMVLLEFVTAAGITLGMDELYK*"
     terminator      4363..4489
                     /label=SV40_PA_terminator
                     /note="/translation=MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATY 
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT 
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD 
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE 
                     KRDHMVLLEFVTAAGITLGMDELYK*"
     misc_feature    4451..4470
                     /label=EBV_rev_primer
                     /note="/translation=MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATY 
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT 
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD 
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE 
                     KRDHMVLLEFVTAAGITLGMDELYK*"
     misc_feature    4490..4495
                     /label=(Kishi does not know the correct sequence aroun...
                     /label=(Kishi does not know the correct sequence around 
                     he(1)
     rep_origin      complement(4718..5337)
                     /direction=LEFT
                     /label=pBR322_origin
                     /note="/translation=MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATY 
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT 
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD 
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE 
                     KRDHMVLLEFVTAAGITLGMDELYK*"
     gene            complement(5492..6352)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="/translation=MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGE
                     GDATY 
                     GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT 
                     IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD 
                     KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE 
                     KRDHMVLLEFVTAAGITLGMDELYK*"
     CDS             complement(5492..6352)
                     /codon_start=1
                     /label=ORF frame 3
                     /label=ORF frame 3(1)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6394..6422)
                     /label=AmpR_promoter
                     /note="/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLG
                     ARVGY 
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE 
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL 
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL 
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA 
                     EIGASLIKHW*"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attccggatc caccatgagc
     1741 gagtcgggct caaagtccag ccagcccctg gcctccaagc aggaaaagga tgggactgag
     1801 aagcgaggcc ggggcaggcc acgcaagcag cctccggtga gtcctgggac ggcgctggtc
     1861 gggagtcaga aagagcccag tgaagtgcca actccgaaga gacctcgggg ccgaccaaag
     1921 ggaagcaaga ataagggcgc cgccaagacc cggaaagtca ccacagctcc agggaggaaa
     1981 ccaaggggca gacccaagaa actggagaag gaggaagagg agggcatctc ccaggagtcc
     2041 tctgaggagg agcagtgaga attcctgcag gcctcgagga tatcgcggcc gctacgtaaa
     2101 ttccgccccc cccccccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     2161 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     2221 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     2281 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     2341 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     2401 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     2461 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     2521 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     2581 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     2641 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     2701 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2761 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2821 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2881 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     2941 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3001 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3061 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3121 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3181 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3241 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3301 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     3361 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     3421 aagcggccgg ccgccagcac agtggtcgat cgacctgcag gcatgcaagc ttcagccaat
     3481 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     3541 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     3601 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     3661 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgccatatg ctggctgcca
     3721 tgaacaaagg tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     3781 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     3841 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     3901 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatgaagat ccctcgacct
     3961 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4021 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4081 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4141 tgtcgtgcca gcnnnnnngc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4201 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4261 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4321 aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat
     4381 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4441 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctn nnnnngctgc
     4501 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     4561 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4621 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4681 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4741 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4801 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4861 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4921 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4981 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5041 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5101 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5161 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5221 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5281 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5341 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5401 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5461 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5521 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5581 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5641 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5701 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5761 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5821 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5881 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5941 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6001 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6061 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6121 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6181 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6241 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6301 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6361 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6421 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6481 ctg
//
