LOCUS       pTV-Klf4-tdiRFP670-PEST 7090 bp ds-DNA     circular     28-MAR-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_recomb     5446..5479
                     /label="FRT"
     rep_origin      1138..1766
                     /label="ColE1 origin"
     primer_bind     complement(6446..6466)
                     /label="T7"
     rep_origin      6650..6956
                     /label="F1 ori"
     CDS             5324..5443
                     /label="PEST sequence"
     primer_bind     2172..2191
                     /label="T3"
     rep_origin      complement(6638..7078)
                     /label="F1 ori"
     promoter        complement(6448..6465)
                     /label="T7_promoter"
     misc_recomb     3299..3332
                     /label="LoxP"
     CDS             4376..5302
                     /label="iRFP670"
     promoter        2171..2189
                     /label="T3 promoter"
     CDS             3374..4300
                     /label="iRFP670"
     CDS             6563..6631
                     /label="LacZ alpha"
     CDS             132..986
                     /label="AmpR"
     primer_bind     complement(6475..6492)
                     /label="M13-fwd"
     primer_bind     2134..2154
                     /label="M13-rev"
     rep_origin      1084..1766
                     /label="ColE1 origin"
     CDS             3245..3298
                     /label="2A peptide"
     misc_feature    2072..2101
                     /label="lac"
     CDS             3341..3361
                     /label="NLS"
     primer_bind     complement(6446..6465)
                     /label="T7"
     CDS             3371..5302
                     /label="tdiRFP670"
     promoter        2072..2102
                     /label="lac promoter"
     misc_feature    59..87
                     /label="Amp prom"
     misc_binding    2106..2128
                     /label="LacO"
ORIGIN
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGCAGCCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG
     1861 AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG
     1921 AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT
     1981 GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG
     2041 TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT
     2101 TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG
     2161 CCAAGCGCGC AATTAACCCT CACTAAAGGG AACAAAAGCT GCGTACGTCT GGTCAAGAAC
     2221 CCGAGTCTCG AAATAACAAA GAACGCACAG GTAGTCTTTT AGTGGGTAGA GTCTTCCAGA
     2281 TGCATTTATG AAAACAGCCA GAACGGCTCT GCTGGAGAAA TTAGCCACGG GTGGCATTCT
     2341 GGACCCAGCG TAATGGCATG GGATCCTTTG GGTTTCAACA GAGCCAACTT CTTTTTTTTT
     2401 TTTTTCCATT TTTTATTAAG TATTTAGCTC ATTTACATTT CCAATGCTAT ACCAAAAGTC
     2461 CCCCATACCC ACCCACCCCC ACTCCCCTAC CCGCCCACTC CCCCTTTTTG GCCCTGGCGT
     2521 TCCCCTGTAC TGGGGCATAT AAAGTTTGCC TGTCCAATGG GCCTCTCTTT CCAGTGATGG
     2581 CCGACTAGGC CATCTTTTGA TATATATGCA ACTAGAGACA AGAGCTCCAG GGTACTGGTT
     2641 AGTTCATAAT GTTGTTCCAC CTATAGGATT GCAGATCCCT TTAGCAGAGC CAACTTCTAA
     2701 TCCAGTCCAT TTCCAGTGCT GGTTAGGCTC GAGAGTGAAA ACTCCGTACT GTGGGGGTGG
     2761 ACGCTTTGAG AGGGTATCTG CTTTTTTTTT TTTTAAGTTA CTAACTACTG TTTCTCCATG
     2821 AGTTTAAACT CATAACACAT GTCATTCCTC TTCATCTGAA ATTCAGTATG GCCCTAAAGT
     2881 GTCATAAAGA CATTCCTGAA GGTTTTTTTT ATTATTATTA TTATATTTTT ACTTTCTTGA
     2941 AAGGAGAGAG GGTGGGGCAT GCAGTAGAAT TTGCTCTCTG GCCTCTCCCT TTTTTTTAAG
     3001 AAAAAAAAGG GGGGTGGCTA ATTCTTCCTT CTAGCAATGG CTTCAGCAGC CCGTTCTCTT
     3061 TCCCTTTACA GGCGAGAAAC CTTACCACTG TGACTGGGAC GGCTGTGGGT GGAAATTCGC
     3121 CCGCTCCGAT GAACTGACCA GGCACTACCG CAAACACACA GGGCACCGGC CCTTTCAGTG
     3181 CCAGAAGTGT GACAGGGCCT TTTCCAGGTC GGACCACCTT GCCTTACACA TGAAGAGGCA
     3241 CTTTGAGGGA AGAGGTAGCC TGCTAACTTG CGGGGACGTA GAGGAAAATC CTGGTCCCAT
     3301 AACTTCGTAT AGCATACATT ATACGAAGTT ATCAATGGGC CCAAAAAAGA AAAGAAAAGT
     3361 TGAATTCATG GCCAGAAAAG TGGACCTGAC CAGTTGTGAC CGAGAGCCGA TACACATTCC
     3421 TGGCTCTATC CAGCCATGTG GGTGTTTGCT GGCCTGCGAT GCCCAGGCAG TCAGGATTAC
     3481 TCGAATTACC GAAAATGCGG GTGCCTTCTT TGGCCGTGAA ACGCCCCGTG TCGGCGAACT
     3541 CTTAGCCGAT TATTTCGGAG AAACCGAGGC TCACGCACTT AGGAACGCAC TGGCGCAATC
     3601 AAGCGATCCC AAACGCCCTG CTCTCATCTT TGGGTGGAGA GATGGACTGA CAGGCCGTAC
     3661 TTTCGACATA AGCCTTCACC GGCATGACGG AACCAGTATA ATTGAGTTTG AACCGGCCGC
     3721 CGCTGAGCAA GCAGACAACC CCCTTCGCCT GACCAGACAA ATTATCGCCC GCACTAAGGA
     3781 GCTGAAATCG CTGGAAGAAA TGGCCGCTCG TGTTCCCAGG TATCTTCAGG CAATGCTGGG
     3841 TTATCATAGG GTAATGCTCT ACAGATTCGC CGACGATGGC TCTGGTATGG TAATAGGGGA
     3901 GGCCAAGCGA TCCGACCTGG AGTCTTTTCT GGGGCAGCAT TTCCCCGCAT CTCTAGTACC
     3961 CCAGCAGGCA AGGCTTTTGT ACCTCAAGAA TGCCATCCGC GTGGTATCGG ACTCCCGCGG
     4021 TATTAGTAGC AGGATCGTTC CAGAACACGA CGCCTCTGGA GCTGCGCTGG ATCTGTCTTT
     4081 CGCTCACCTC CGATCTATCA GCCCTTGTCA TTTAGAGTTT CTGCGAAATA TGGGGGTCAG
     4141 CGCAAGTATG TCCCTGAGCA TAATAATCGA TGGAACACTA TGGGGCCTAA TAATTTGCCA
     4201 TCATTACGAA CCTAGAGCAG TACCAATGGC ACAGCGAGTG GCCGCCGAAA TGTTCGCCGA
     4261 TTTTTTGTCT CTTCATTTTA CTGCCGCTCA CCATCAGCGC GGCCACGGGA CTGGCTCGAC
     4321 AGGTTCTGGC AGTAGTGGGA CTGCTTCATC AGAAGATAAT AACATGGCCA TGGCCAGAAA
     4381 AGTGGACCTG ACCAGTTGTG ACCGAGAGCC GATACACATT CCTGGCTCTA TCCAGCCATG
     4441 TGGGTGTTTG CTGGCCTGCG ATGCCCAGGC AGTCAGGATT ACTCGAATTA CCGAAAATGC
     4501 GGGTGCCTTC TTTGGCCGTG AAACGCCCCG TGTCGGCGAA CTCTTAGCCG ATTATTTCGG
     4561 AGAAACCGAG GCTCACGCAC TTAGGAACGC ACTGGCGCAA TCAAGCGATC CCAAACGCCC
     4621 TGCTCTCATC TTTGGGTGGA GAGATGGACT GACAGGCCGT ACTTTCGACA TAAGCCTTCA
     4681 CCGGCATGAC GGAACCAGTA TAATTGAGTT TGAACCGGCC GCCGCTGAGC AAGCAGACAA
     4741 CCCCCTTCGC CTGACCAGAC AAATTATCGC CCGCACTAAG GAGCTGAAAT CGCTGGAAGA
     4801 AATGGCCGCT CGTGTTCCCA GGTATCTTCA GGCAATGCTG GGTTATCATA GGGTAATGCT
     4861 CTACAGATTC GCCGACGATG GCTCTGGTAT GGTAATAGGG GAGGCCAAGC GATCCGACCT
     4921 GGAGTCTTTT CTGGGGCAGC ATTTCCCCGC ATCTCTAGTA CCCCAGCAGG CAAGGCTTTT
     4981 GTACCTCAAG AATGCCATCC GCGTGGTATC GGACTCCCGC GGTATTAGTA GCAGGATCGT
     5041 TCCAGAACAC GACGCCTCTG GAGCTGCGCT GGATCTGTCT TTCGCTCACC TCCGATCTAT
     5101 CAGCCCTTGT CATTTAGAGT TTCTGCGAAA TATGGGGGTC AGCGCAAGTA TGTCCCTGAG
     5161 CATAATAATC GATGGAACAC TATGGGGCCT AATAATTTGC CATCATTACG AACCTAGAGC
     5221 AGTACCAATG GCACAGCGAG TGGCCGCCGA AATGTTCGCC GATTTTTTGT CTCTTCATTT
     5281 TACTGCCGCT CACCATCAGC GCGGATCCGC GTTTAAACTC GAGTCTCACG GCTTCCCTCC
     5341 CGAGGTGGAG GAGCAGGCCG CCGGCACCCT GCCCATGAGC TGCGCCCAGG AGAGCGGCAT
     5401 GGATAGACAC CCTGCTGCTT GCGCCAGCGC CAGGATCAAC GTCTTGAAGT TCCTATTCTC
     5461 TAGAAAGTAT AGGAACTTCT AGATCCCACG TAGTGGATGT GACCCACACT GCCAGGAGAG
     5521 AGAGTTCAGT ATTTTTTTTT CTAACCTTTC ACACTGTCTT CCCACGAGGG GAGGAGCCCA
     5581 GCTGGCAAGC GCTACAATCA TGGTCAAGTT CCCAGCAAGT CAGCTTGTGA ATGGATAATC
     5641 AGGAGAAAGG AAGAGTTCAA GAGACAAAAC AGAAATACTA AAAACAAACA AACAAAAAAA
     5701 CAAACAAAAA AAACAAGAAA AAAAAATCAC AGAACAGATG GGGTCTGATA CTGGATGGAT
     5761 CTTCTATCAT TCCAATACCA AATCCAACTT GAACATGCCC GGACTTACAA AATGCCAAGG
     5821 GGTGACTGGA AGTTTGTGGA TATCAGGGTA TACACTAAAT CAGTGAGCTT GGGGGGAGGG
     5881 AAGACCAGGA TTCCCTTGAA TTGTGTTTCG ATGATGCAAT ACACACGTAA AGATCACCTT
     5941 GTATGCTCTT TGCCTTCTTA AAAAAAAAAA AAGCCATTAT TGTGTCGGAG GAAGAGGAAG
     6001 CGATTCAGGT ACAGAACATG TTCTAACAGC CTAAATGATG GTGCTTGGTG AGTCGTGGTT
     6061 CTAAAGGTAC CAAACGGGGG AGCCAAAGTT CTCCAACTGC TGCATACTTT TGACAAGGAA
     6121 AATCTAGTTT TGTCTTCCGA TCTACATTGA TGACCTAAGC CAGGTAAATA AGCCTGGTTT
     6181 ATTTCTGTAA CATTTTTATG CAGACAGTCT GTTATGCACT GTGGTTTCAG ATGTGCAATA
     6241 ATTTGTACAA TGGTTTATTC CCAAGTATGC CTTTAAGCAG AACAAATGTG TTTTTCTATA
     6301 TAGTTCCTTG CCTTAATAAA TATGTAATAT AAATTTAAGC AAACTTCTAT TTTGTATATT
     6361 TGTAAACTAC AAAGTAAAAA AAAATGAACA TTTTGTGGAG TTTGTATTTT GCATACTCAA
     6421 GGTGAGAAAT AAGAAGCTCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC GCTCACTGGC
     6481 CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC
     6541 AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC
     6601 CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC TGTAGCGGCG CATTAAGCGC
     6661 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC
     6721 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT
     6781 AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA
     6841 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC
     6901 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT
     6961 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG
     7021 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT
     7081 TACAATTTAG
//