LOCUS       pTV-Npm1-tdiRFP670-PEST 7055 bp ds-DNA     circular     08-FEB-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(4955..4984)
                     /label="lac"
     promoter        complement(4867..4885)
                     /label="T3 promoter"
     primer_bind     625..645
                     /label="T7"
     promoter        626..643
                     /label="T7_promoter"
     CDS             1646..1699
                     /label="2A peptide"
     misc_recomb     1700..1733
                     /label="LoxP"
     CDS             complement(6070..6924)
                     /label="AmpR"
     promoter        complement(4954..4984)
                     /label="lac promoter"
     misc_recomb     3847..3880
                     /label="FRT"
     CDS             1775..2701
                     /label="iRFP670"
     CDS             1772..3703
                     /label="tdiRFP670"
     misc_binding    complement(4928..4950)
                     /label="LacO"
     CDS             3725..3844
                     /label="PEST sequence"
     misc_feature    complement(6969..6997)
                     /label="Amp prom"
     primer_bind     complement(4865..4884)
                     /label="T3"
     rep_origin      13..453
                     /label="F1 ori"
     primer_bind     complement(4902..4922)
                     /label="M13-rev"
     primer_bind     599..616
                     /label="M13-fwd"
     CDS             2777..3703
                     /label="iRFP670"
     rep_origin      complement(135..441)
                     /label="F1 ori"
     rep_origin      complement(5290..5918)
                     /label="ColE1 origin"
     CDS             1742..1762
                     /label="NLS"
     rep_origin      complement(5290..5972)
                     /label="ColE1 origin"
     CDS             complement(460..528)
                     /label="LacZ alpha"
     primer_bind     626..645
                     /label="T7"
ORIGIN
        1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
      121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
      181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
      241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
      301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
      361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
      421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG
      481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG
      541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG
      601 TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGGGTACCTT
      661 AAATAGTTGG TAAATTATCT CTTGGTTACT GAACTTAGTT TTACTCTTAG TAGTGTATTG
      721 GCTAAGGAAA TAGGATATGG TTTTTATTTG CTCCATTTGG CTGCTTTACA GTCTGATGAT
      781 ACTTGGACTC TTGGAAGTGG TAGTATCTTC TCCAGATAGC AAAGGAGGGT AAAATTGGTA
      841 GAATCAACCT AGATGGGTTA AAGCCTCTTG CATGTCAAGT TACTGTTATG TTTATTGTGG
      901 TATTAGGATA CTTGGGCCAT GCAAGGGCAA AGCACTACCA GTGATCTCTT CCCAGTGGTA
      961 TGAATTTACA GTGTTAAATA GTAAATATAA TGGGTTAATG TTAGACCTTC TGAATGAACA
     1021 GTTGATTTAA TTGGTTTCAT CAGTATTGGC TATAGAGGTG TCTATTCAAA AAGAATTAAA
     1081 AGGAACAATA TAGCAGTAGA GTATTAAATG ATAGTATTGC CTATTTAAAT TATAGCTCCC
     1141 AGGTTTGTAG TCAAAAGTGG TTAGGATTGA GGCAGACTGT CAATGATTGG AAGTAGTGAT
     1201 GTCATCAAAT TCAGTCCTTT CGTGTTAGCT ACAGATTGAC CTAAGTTTTA GAATTCACCA
     1261 CTGAAGAGAA TACTTGATAT TTTTAAGTTT TTAGAAACAT ACTGGCTTGT CTTAAGTATG
     1321 TCCCTTTGGG TTTTTCTAGG TTGTGATTTT TTTTTTTTTT TGCTTTGTAA TACTTTGCCT
     1381 AGACTTTAAA CATTCTCACT CAAATTCTGA AACCTAAATA GGGCTGACCC ACAGGCTGAG
     1441 GAGGCAACAC TGGCCATAAA GTATTTAAGC CATTTAATTA AGGAAACTCA TGCTATGAGT
     1501 TAATTCTGTA TTTTTCCTTT GTAAAATGAA AAATATTTAA CTGAAGAAAT GAAATTTGTT
     1561 TAATTCTATA AAGTTAACAT TTCTTTACCA TTTTTTTCCT CCTTTACAGG CTATTCAAGA
     1621 TCTCTGGCAG TGGAGGAAAT CTCTTGAGGG AAGAGGTAGC CTGCTAACTT GCGGGGACGT
     1681 AGAGGAAAAT CCTGGTCCCA TAACTTCGTA TAGCATACAT TATACGAAGT TATCAATGGG
     1741 CCCAAAAAAG AAAAGAAAAG TTGAATTCAT GGCCAGAAAA GTGGACCTGA CCAGTTGTGA
     1801 CCGAGAGCCG ATACACATTC CTGGCTCTAT CCAGCCATGT GGGTGTTTGC TGGCCTGCGA
     1861 TGCCCAGGCA GTCAGGATTA CTCGAATTAC CGAAAATGCG GGTGCCTTCT TTGGCCGTGA
     1921 AACGCCCCGT GTCGGCGAAC TCTTAGCCGA TTATTTCGGA GAAACCGAGG CTCACGCACT
     1981 TAGGAACGCA CTGGCGCAAT CAAGCGATCC CAAACGCCCT GCTCTCATCT TTGGGTGGAG
     2041 AGATGGACTG ACAGGCCGTA CTTTCGACAT AAGCCTTCAC CGGCATGACG GAACCAGTAT
     2101 AATTGAGTTT GAACCGGCCG CCGCTGAGCA AGCAGACAAC CCCCTTCGCC TGACCAGACA
     2161 AATTATCGCC CGCACTAAGG AGCTGAAATC GCTGGAAGAA ATGGCCGCTC GTGTTCCCAG
     2221 GTATCTTCAG GCAATGCTGG GTTATCATAG GGTAATGCTC TACAGATTCG CCGACGATGG
     2281 CTCTGGTATG GTAATAGGGG AGGCCAAGCG ATCCGACCTG GAGTCTTTTC TGGGGCAGCA
     2341 TTTCCCCGCA TCTCTAGTAC CCCAGCAGGC AAGGCTTTTG TACCTCAAGA ATGCCATCCG
     2401 CGTGGTATCG GACTCCCGCG GTATTAGTAG CAGGATCGTT CCAGAACACG ACGCCTCTGG
     2461 AGCTGCGCTG GATCTGTCTT TCGCTCACCT CCGATCTATC AGCCCTTGTC ATTTAGAGTT
     2521 TCTGCGAAAT ATGGGGGTCA GCGCAAGTAT GTCCCTGAGC ATAATAATCG ATGGAACACT
     2581 ATGGGGCCTA ATAATTTGCC ATCATTACGA ACCTAGAGCA GTACCAATGG CACAGCGAGT
     2641 GGCCGCCGAA ATGTTCGCCG ATTTTTTGTC TCTTCATTTT ACTGCCGCTC ACCATCAGCG
     2701 CGGCCACGGG ACTGGCTCGA CAGGTTCTGG CAGTAGTGGG ACTGCTTCAT CAGAAGATAA
     2761 TAACATGGCC ATGGCCAGAA AAGTGGACCT GACCAGTTGT GACCGAGAGC CGATACACAT
     2821 TCCTGGCTCT ATCCAGCCAT GTGGGTGTTT GCTGGCCTGC GATGCCCAGG CAGTCAGGAT
     2881 TACTCGAATT ACCGAAAATG CGGGTGCCTT CTTTGGCCGT GAAACGCCCC GTGTCGGCGA
     2941 ACTCTTAGCC GATTATTTCG GAGAAACCGA GGCTCACGCA CTTAGGAACG CACTGGCGCA
     3001 ATCAAGCGAT CCCAAACGCC CTGCTCTCAT CTTTGGGTGG AGAGATGGAC TGACAGGCCG
     3061 TACTTTCGAC ATAAGCCTTC ACCGGCATGA CGGAACCAGT ATAATTGAGT TTGAACCGGC
     3121 CGCCGCTGAG CAAGCAGACA ACCCCCTTCG CCTGACCAGA CAAATTATCG CCCGCACTAA
     3181 GGAGCTGAAA TCGCTGGAAG AAATGGCCGC TCGTGTTCCC AGGTATCTTC AGGCAATGCT
     3241 GGGTTATCAT AGGGTAATGC TCTACAGATT CGCCGACGAT GGCTCTGGTA TGGTAATAGG
     3301 GGAGGCCAAG CGATCCGACC TGGAGTCTTT TCTGGGGCAG CATTTCCCCG CATCTCTAGT
     3361 ACCCCAGCAG GCAAGGCTTT TGTACCTCAA GAATGCCATC CGCGTGGTAT CGGACTCCCG
     3421 CGGTATTAGT AGCAGGATCG TTCCAGAACA CGACGCCTCT GGAGCTGCGC TGGATCTGTC
     3481 TTTCGCTCAC CTCCGATCTA TCAGCCCTTG TCATTTAGAG TTTCTGCGAA ATATGGGGGT
     3541 CAGCGCAAGT ATGTCCCTGA GCATAATAAT CGATGGAACA CTATGGGGCC TAATAATTTG
     3601 CCATCATTAC GAACCTAGAG CAGTACCAAT GGCACAGCGA GTGGCCGCCG AAATGTTCGC
     3661 CGATTTTTTG TCTCTTCATT TTACTGCCGC TCACCATCAG CGCGGATCCG CGTTTAAACT
     3721 CGAGTCTCAC GGCTTCCCTC CCGAGGTGGA GGAGCAGGCC GCCGGCACCC TGCCCATGAG
     3781 CTGCGCCCAG GAGAGCGGCA TGGATAGACA CCCTGCTGCT TGCGCCAGCG CCAGGATCAA
     3841 CGTCTTGAAG TTCCTATTCT CTAGAAAGTA TAGGAACTTC TAGTAAGAAA AGGGTTTAAA
     3901 CAGTTTGAAA TATTCTGTCT TCATTTCTGT AATAGTTAAT ATCTGGCTGT CCTTTTTATA
     3961 ATGCAAAGTG AGAACTTTCC CTACTGTGTT TGATAAATGT TGTCCAGGTT CACTTGCCAA
     4021 GAATGTGTTG TCTAAAATGC CTGTTTAGTT TTCAAGGATG GAACTCCACC CTTTACTTGG
     4081 TTTTAAGTAT GTATGGAATG TTATGATAGG ACATAGTAAT AGTGGTCAGA TGTGGAAATG
     4141 GTAGGGAGAC AAATATACAT GTGAAATAAA CTCAGTATTT TAATAAAGTA GCACTGTTTC
     4201 TATTTGCTTA TTTAACTCTC ATACTTTGAA GTTAACGTTA GTGCCCTCAT TTCTGAGCAG
     4261 AATCACTGTT CATACATTCC CATTTAGGAA CTTAACACTT AAAACACACC TTTCCTCTAT
     4321 TGATAGCCAA TCTGTTGGAC ATAACCATAG GATTTATTCA TCCTGTTTTG CTGTGGTATA
     4381 TTGTAACAAT GCATGGGAAA ATACATACTT AAGAAAAAAA CTCAGTGGAT AGTGTCTTTC
     4441 ACAAACACTC AGGCTATCTG TATGGATGCT TTTCTTGAAT CCTTTTAAAA TCTGGTTTGG
     4501 AATTGAGTCT AAAGTTGCCT AGCAGGTCAT ATGGGGTAGG TTAAAAGAGG TATGGTATAG
     4561 TTTGTCTATT CCTGCTCAAG TTTGAAGTAA CTTTAGTCAA AGGCTGCTTC GTGCCTGAAC
     4621 CTTTTTTTTT TTTTTTGGTT TTTCGAGACA GGGCTTCTCT GTATAGCCCT GTCCTGTAAA
     4681 CCGGGCTGGC CTCAGAAATC TGCCTGCCTC TGCCTCCTGA GTGCTGGGAT TAAAGATGTG
     4741 TGCCACCACG CCTGGCTCCT GAACCTCTTT ATAGTGAGGA AATTCTATAC ATATAGGTGT
     4801 ATAAAACTAT TTGGTAACAG ATTAGACTAA AATCTGCATA TTAAGCTGGA GCTCCAGCTT
     4861 TTGTTCCCTT TAGTGAGGGT TAATTGCGCG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC
     4921 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG
     4981 TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC
     5041 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG
     5101 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
     5161 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
     5221 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
     5281 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     5341 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     5401 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     5461 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     5521 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     5581 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     5641 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     5701 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG
     5761 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     5821 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     5881 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     5941 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     6001 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     6061 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     6121 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     6181 TGGCCCCAGT GCTGCAATGA TACCGCGGCT GCCACGCTCA CCGGCTCCAG ATTTATCAGC
     6241 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     6301 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     6361 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     6421 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     6481 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     6541 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     6601 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     6661 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     6721 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     6781 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     6841 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     6901 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     6961 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     7021 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCAC
//