LOCUS       pTV-Birc6-tdiRFP670-PES 7103 bp ds-DNA     circular     20-FEB-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     complement(4950..4970)
                     /label="M13-rev"
     CDS             complement(6118..6972)
                     /label="AmpR"
     CDS             3715..3834
                     /label="PEST sequence"
     CDS             1765..2691
                     /label="iRFP670"
     misc_recomb     1690..1723
                     /label="LoxP"
     misc_binding    complement(4976..4998)
                     /label="LacO"
     misc_feature    complement(7017..7045)
                     /label="Amp prom"
     primer_bind     625..645
                     /label="T7"
     CDS             2767..3693
                     /label="iRFP670"
     promoter        complement(4915..4933)
                     /label="T3 promoter"
     rep_origin      complement(5338..5966)
                     /label="ColE1 origin"
     primer_bind     complement(4913..4932)
                     /label="T3"
     rep_origin      complement(5338..6020)
                     /label="ColE1 origin"
     promoter        626..643
                     /label="T7_promoter"
     primer_bind     599..616
                     /label="M13-fwd"
     CDS             1762..3693
                     /label="tdiRFP670"
     CDS             complement(460..528)
                     /label="LacZ alpha"
     rep_origin      13..453
                     /label="F1 ori"
     misc_feature    complement(5003..5032)
                     /label="lac"
     CDS             1732..1752
                     /label="NLS"
     promoter        complement(5002..5032)
                     /label="lac promoter"
     primer_bind     626..645
                     /label="T7"
     rep_origin      complement(135..441)
                     /label="F1 ori"
     misc_recomb     3837..3870
                     /label="FRT"
     CDS             1636..1689
                     /label="2A peptide"
ORIGIN
        1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
      121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
      181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
      241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
      301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
      361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
      421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG
      481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG
      541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG
      601 TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGGGTACCCC
      661 TTGCTCGTGC CAGAGAAGTG CTGCACTATT CAATCAACAC CCAATCTGGG TATATTTTAG
      721 TTTGTTCAGT TGTCCTTTTC AGAGTTGGCA TCTGTGACAG CACAAGGAAT TACTGTCTTT
      781 AATTTAAAAG AATATGTATA TCAAACTATT GATGTTTGTA AGTCATTGTT GATGCTGATC
      841 CTGTGGAACT GTACTGTGTT CTTGAGGAGG TGGAGTTTAG TCTTTTGTAG CTGGATAGAA
      901 TGTAACTCTG AGCTTTTCTT TTCTTTTTTT TTTTTCTTTT TTCTTTTTTT TGGTTTTTTG
      961 AGACAGGGTT TCTCTGTGTA GCCCTGGCTG TCCTGGAACT CACTTTGTAG ACCAGGCTGG
     1021 CCACGAACTC AGAAATCTGC CTGCCTCTGC CTCCCAAGTG CTGGGATTAA AGGCGCCACC
     1081 ACACCCAGCT TACTCTGAGC TTTTCAACAT CTGGAATTAA TTTCTATGTT TAGGAATTTT
     1141 GCCTGCATGT ATATGCATAT GGGGGTGGTG TGAGCCCTGG AACTTGAGTT ACAGACAGGT
     1201 GTGAATTACC ATGTGGGTGC TGGGAATTGA ACCAGGGTCC TCATGAAAAA TAGCTAGTGC
     1261 TCTTAACCTC TGAACCATCT CTCTAGCCCC TAGTTTGGGG ATCCTTGAGA GTTTTGTTTA
     1321 CTGTGTTTTA AATGGCTGCC ATAAGAAGAC AGTAAAGATT TGTACTCGAT ACTGAAGTAT
     1381 GTTAAAACCA AGTTGTCTCA GTGATAGGCA AGAATCACAC TGTTCACACT TCTGATATTA
     1441 GCGATTTTCT CTACCTAGCG CCACACTGCT CAGCTCCGAG AAGAGCTACT GAAACTTCCC
     1501 TGCCCTGAAG GCTTAGATCC GGACATTGAG GATGCCTCCC CGGTGTGCAG GGCCACAGCA
     1561 GGTGCTGAAG ACACTCTAAC CCACGACCAT GTCAATCCCA GCAGCAGCAA AGACCTCCCC
     1621 AGTGACTTCC AATTAGAGGG AAGAGGTAGC CTGCTAACTT GCGGGGACGT AGAGGAAAAT
     1681 CCTGGTCCCA TAACTTCGTA TAGCATACAT TATACGAAGT TATCAATGGG CCCAAAAAAG
     1741 AAAAGAAAAG TTGAATTCAT GGCCAGAAAA GTGGACCTGA CCAGTTGTGA CCGAGAGCCG
     1801 ATACACATTC CTGGCTCTAT CCAGCCATGT GGGTGTTTGC TGGCCTGCGA TGCCCAGGCA
     1861 GTCAGGATTA CTCGAATTAC CGAAAATGCG GGTGCCTTCT TTGGCCGTGA AACGCCCCGT
     1921 GTCGGCGAAC TCTTAGCCGA TTATTTCGGA GAAACCGAGG CTCACGCACT TAGGAACGCA
     1981 CTGGCGCAAT CAAGCGATCC CAAACGCCCT GCTCTCATCT TTGGGTGGAG AGATGGACTG
     2041 ACAGGCCGTA CTTTCGACAT AAGCCTTCAC CGGCATGACG GAACCAGTAT AATTGAGTTT
     2101 GAACCGGCCG CCGCTGAGCA AGCAGACAAC CCCCTTCGCC TGACCAGACA AATTATCGCC
     2161 CGCACTAAGG AGCTGAAATC GCTGGAAGAA ATGGCCGCTC GTGTTCCCAG GTATCTTCAG
     2221 GCAATGCTGG GTTATCATAG GGTAATGCTC TACAGATTCG CCGACGATGG CTCTGGTATG
     2281 GTAATAGGGG AGGCCAAGCG ATCCGACCTG GAGTCTTTTC TGGGGCAGCA TTTCCCCGCA
     2341 TCTCTAGTAC CCCAGCAGGC AAGGCTTTTG TACCTCAAGA ATGCCATCCG CGTGGTATCG
     2401 GACTCCCGCG GTATTAGTAG CAGGATCGTT CCAGAACACG ACGCCTCTGG AGCTGCGCTG
     2461 GATCTGTCTT TCGCTCACCT CCGATCTATC AGCCCTTGTC ATTTAGAGTT TCTGCGAAAT
     2521 ATGGGGGTCA GCGCAAGTAT GTCCCTGAGC ATAATAATCG ATGGAACACT ATGGGGCCTA
     2581 ATAATTTGCC ATCATTACGA ACCTAGAGCA GTACCAATGG CACAGCGAGT GGCCGCCGAA
     2641 ATGTTCGCCG ATTTTTTGTC TCTTCATTTT ACTGCCGCTC ACCATCAGCG CGGCCACGGG
     2701 ACTGGCTCGA CAGGTTCTGG CAGTAGTGGG ACTGCTTCAT CAGAAGATAA TAACATGGCC
     2761 ATGGCCAGAA AAGTGGACCT GACCAGTTGT GACCGAGAGC CGATACACAT TCCTGGCTCT
     2821 ATCCAGCCAT GTGGGTGTTT GCTGGCCTGC GATGCCCAGG CAGTCAGGAT TACTCGAATT
     2881 ACCGAAAATG CGGGTGCCTT CTTTGGCCGT GAAACGCCCC GTGTCGGCGA ACTCTTAGCC
     2941 GATTATTTCG GAGAAACCGA GGCTCACGCA CTTAGGAACG CACTGGCGCA ATCAAGCGAT
     3001 CCCAAACGCC CTGCTCTCAT CTTTGGGTGG AGAGATGGAC TGACAGGCCG TACTTTCGAC
     3061 ATAAGCCTTC ACCGGCATGA CGGAACCAGT ATAATTGAGT TTGAACCGGC CGCCGCTGAG
     3121 CAAGCAGACA ACCCCCTTCG CCTGACCAGA CAAATTATCG CCCGCACTAA GGAGCTGAAA
     3181 TCGCTGGAAG AAATGGCCGC TCGTGTTCCC AGGTATCTTC AGGCAATGCT GGGTTATCAT
     3241 AGGGTAATGC TCTACAGATT CGCCGACGAT GGCTCTGGTA TGGTAATAGG GGAGGCCAAG
     3301 CGATCCGACC TGGAGTCTTT TCTGGGGCAG CATTTCCCCG CATCTCTAGT ACCCCAGCAG
     3361 GCAAGGCTTT TGTACCTCAA GAATGCCATC CGCGTGGTAT CGGACTCCCG CGGTATTAGT
     3421 AGCAGGATCG TTCCAGAACA CGACGCCTCT GGAGCTGCGC TGGATCTGTC TTTCGCTCAC
     3481 CTCCGATCTA TCAGCCCTTG TCATTTAGAG TTTCTGCGAA ATATGGGGGT CAGCGCAAGT
     3541 ATGTCCCTGA GCATAATAAT CGATGGAACA CTATGGGGCC TAATAATTTG CCATCATTAC
     3601 GAACCTAGAG CAGTACCAAT GGCACAGCGA GTGGCCGCCG AAATGTTCGC CGATTTTTTG
     3661 TCTCTTCATT TTACTGCCGC TCACCATCAG CGCGGATCCG CGTTTAAACT CGAGTCTCAC
     3721 GGCTTCCCTC CCGAGGTGGA GGAGCAGGCC GCCGGCACCC TGCCCATGAG CTGCGCCCAG
     3781 GAGAGCGGCA TGGATAGACA CCCTGCTGCT TGCGCCAGCG CCAGGATCAA CGTCTTGAAG
     3841 TTCCTATTCT CTAGAAAGTA TAGGAACTTC TAGTGAGCTG CATTGATGTG GATTTCGTAG
     3901 ACACAAAGGC TTCGAAGCAC AAGCCAAATA TGTCAATATT TGTATGTAAG AAACTAATTA
     3961 TGTAATAGGT AATGAAACTG AAACTATACT ATGCCCTTAA GGAGATCCAG TTTAATTCAA
     4021 GGTGATCTTT GATTTACCTG TACAAGAGTG TAAACTTTTT TGTGCTTTTA TTTTTCAATT
     4081 GTGAGAACCA CTGATTGGTA TGTTCAACAA ATTTGTGTAT ACAAAAAAAT GGATAAATCA
     4141 CTGATCTATA AGGGAAACTA CCTTAGGAAA GAATGGTTAC TGAATGTTTA TCTTTTTTTT
     4201 TTTTTTTTTT TTTTTTTTAC TGTAGAGTGA GAGGTTGTTA ACAAAGAATA TATATTGGTC
     4261 ATTCTTACAA CTACTATTTA AAGTCAGCAA CTTTTCACTG AATTTGATAG ATTTTGTTTG
     4321 GCCATATCTT CATGCTCATA TTTGATTTCT GAAGACTTCC TACATACACT TTAATAAAAG
     4381 TTAAACAGAC ATAGCCCCTC TTTTTTGATT TACTGGTACA TTTTTAAGTA ATAAATCTGC
     4441 CATAAAATGC ACTATAGCTG GAGACTTGCA CTTGTATGGA TGAATTTATT ACATTCAACA
     4501 TATTTAATTT TATGCCTTCT AAGATGCAGA AAAAAATAAA TGAACATGAT TTTATTCTAT
     4561 GCCAAACATT TGGGCCTCTG AATGTATCCA TTATTTGAAT TTACTATATG AAAAGGTATG
     4621 GTCAATTGGA AAAGTCGCTT TAAACTGTTT TCCTCAAGGG CTCCTCTTAT TCCTGGACTG
     4681 TGTGGGTTTG TTCCTGAAGT CACATGTATG TATCAACATG GAGGCTCTGT AGAGGAGAGA
     4741 GGCTGGGCCT CTCTGGTGCT GTTACAGTAC GTTCTGTACC TGCCATACAG GCTCGTTTTC
     4801 ATGCAAATTC TTCCTAGAGC CAATAAATAA AAATGTAGGT AAATTAGTAT GTCTTGTTTC
     4861 TAAAATAGTT GCCATGGTGT GCAATGGTAT TTCTACGAGC TCCAGCTTTT GTTCCCTTTA
     4921 GTGAGGGTTA ATTGCGCGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG
     4981 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG
     5041 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC
     5101 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT
     5161 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT
     5221 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA
     5281 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
     5341 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     5401 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     5461 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     5521 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     5581 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     5641 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     5701 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     5761 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
     5821 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     5881 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     5941 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
     6001 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
     6061 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
     6121 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC
     6181 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC
     6241 TGCAATGATA CCGCGGCTGC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
     6301 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT
     6361 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
     6421 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC
     6481 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG
     6541 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
     6601 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC
     6661 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
     6721 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT
     6781 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC
     6841 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
     6901 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA
     6961 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
     7021 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG
     7081 CACATTTCCC CGAAAAGTGC CAC
//