LOCUS       Exported                5540 bp ds-DNA     circular SYN 20-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 20, 2018 from SnapGene Viewer 4.1.5
            http://www.snapgene.com
COMMENT     Promoter from p5E-huBetaB1Cry300P 675 to 990
            Gene from 768. pME-mAppleCAAX 1 to 804
            UTR from 302. p3E-polyA 734 to 953
            Backbone from 394. pDestTol2pA2 2787 to 1103
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            228..514
                     /locus_tag="medaka tyrosinase genomic (from AB010101)"
                     /label=medaka tyrosinase genomic (from AB010101)
     LTR             complement(514..530)
                     /locus_tag="Tol2 3' TIR"
                     /label=Tol2 3' TIR
     misc_structure  complement(703..1049)
                     /locus_tag="Tol2 exon4 136-482"
                     /label=Tol2 exon4 136-482
     primer_bind     1061..1082
                     /locus_tag="M13R(1)"
                     /label=M13R
                     /label=M13R(1)
     misc_recomb     1097..1103
                     /locus_tag="attB4"
                     /label=attB4
     misc_recomb     1097..1103
                     /locus_tag="attR4"
                     /label=attR4
     misc_feature    1097..1103
                     /locus_tag="att4_shared"
                     /label=att4_shared
     misc_feature    1104..1117
                     /locus_tag="att site in primer"
                     /label=att site in primer
     misc_feature    1118..1412
                     /locus_tag="Human beta B1 300bp promoter"
                     /label=Human beta B1 300bp promoter
     misc_feature    1413..1419
                     /locus_tag="att site primer"
                     /label=att site primer
     misc_recomb     1420..1436
                     /locus_tag="attL1"
                     /label=attL1
     misc_feature    1420..1430
                     /locus_tag="att1_shared"
                     /label=att1_shared
     CDS             1442..2149
                     /locus_tag="mApple"
                     /label=mApple
     CDS             1442..2149
                     /locus_tag="mApple (Davidson)"
                     /label=mApple (Davidson)
     misc_structure  2150..2212
                     /locus_tag="CAAX box from Harvey ras"
                     /label=CAAX box from Harvey ras
     misc_recomb     2214..2223
                     /locus_tag="attL2"
                     /label=attL2
     misc_feature    2220..2223
                     /locus_tag="att2_shared(1)"
                     /label=att2_shared
                     /label=att2_shared(1)
     misc_recomb     2224..2237
                     /locus_tag="attR2"
                     /label=attR2
     misc_feature    2224..2235
                     /locus_tag="att2_shared"
                     /label=att2_shared
     polyA_signal    2240..2435
                     /locus_tag="SV40 late polyA (from pCS2+)"
                     /label=SV40 late polyA (from pCS2+)
     misc_recomb     2437..2443
                     /locus_tag="attL3"
                     /label=attL3
     misc_feature    2437..2443
                     /locus_tag="att3_shared"
                     /label=att3_shared
     misc_recomb     2444..2457
                     /locus_tag="attB3"
                     /label=attB3
     misc_recomb     2444..2457
                     /locus_tag="attR3"
                     /label=attR3
     misc_feature    2444..2451
                     /locus_tag="att3_shared(1)"
                     /label=att3_shared
                     /label=att3_shared(1)
     primer_bind     complement(2466..2481)
                     /locus_tag="M13F"
                     /label=M13F
     polyA_signal    2497..2694
                     /locus_tag="SV40 late polyA (from pCS2+)(1)"
                     /label=SV40 late polyA (from pCS2+)
                     /label=SV40 late polyA (from pCS2+)(1)
     misc_structure  complement(2753..3087)
                     /locus_tag="Tol2 exon1 bp1-335 of 461"
                     /label=Tol2 exon1 bp1-335 of 461
     LTR             complement(3251..3269)
                     /locus_tag="Tol2 5' TIR"
                     /label=Tol2 5' TIR
     misc_structure  complement(3270..3306)
                     /locus_tag="medaka tyrosinase ex5 frag"
                     /label=medaka tyrosinase ex5 frag
     promoter        complement(3355..3374)
                     /locus_tag="T3"
                     /label=T3
     primer_bind     complement(3390..3411)
                     /locus_tag="M13R"
                     /label=M13R
     rep_origin      complement(3738..4411)
                     /direction=LEFT
                     /locus_tag="pUC ori"
                     /label=pUC ori
     rep_origin      complement(3779..4461)
                     /direction=LEFT
                     /locus_tag="colE1 ori"
                     /label=colE1 ori
     CDS             complement(4559..5416)
                     /locus_tag="ampR"
                     /label=ampR
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttgatc tgtcagggta gggttggaag
     1141 gtgaagctgc tgtggccagg gtttctgctg tgtgtgctgg tctcacaaag gaaagtgctg
     1201 ctgtctgcag tgatccaggc gaggtcaggg ccccagtccc ccactccctg cccaggcctg
     1261 ctgagccagc ttcccctttg tgccacagac tatagggcct gatgtctgcc agactataaa
     1321 atggggggtc ggactctagt cacccatagc acctgcccgc ctgcctgtcc tccagcagga
     1381 agtagcaagt ccccaggtaa gagggaaccc accaagtttg tacaaaaaag caggctggac
     1441 catggtgagc aagggcgagg agaataacat ggccatcatc aaggagttca tgcgcttcaa
     1501 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     1561 ccgcccctac gaggcctttc agaccgctaa gctgaaggtg accaagggtg gccccctgcc
     1621 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggtct acattaagca
     1681 cccagccgac atccccgact acttcaagct gtccttcccc gagggcttca ggtgggagcg
     1741 cgtgatgaac ttcgaggacg gcggcattat tcacgttaac caggactcct ccctgcagga
     1801 cggcgtgttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     1861 aatgcagaag aagaccatgg gctgggaggc ctccgaggag cggatgtacc ccgaggacgg
     1921 cgccctgaag agcgagatca agaagaggct gaagctgaag gacggcggcc actacgccgc
     1981 cgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt
     2041 cgacatcaag ttggacatcg tgtcccacaa cgaggactac accatcgtgg aacagtacga
     2101 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaaga agctgaaccc
     2161 tcctgatgag agtggccccg gctgcatgag ctgcaagtgt gtgctctcct gacacccagc
     2221 tttcttgtac aaagtggggg atccagacat gataagatac attgatgagt ttggacaaac
     2281 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     2341 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     2401 gtttcaggtt cagggggagg tgtgggaggt tttttccaac tttattatac atagttgata
     2461 attcactggc cgtcgtttta cggtaccatc gatgatgatc cagacatgat aagatacatt
     2521 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     2581 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     2641 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     2701 taaaacctct acaaatgtgg tatggctgat tatgatcctc tagatcagat ctgcgaagat
     2761 acggccacgg gtgctcttga tcctgtggct gattttggac tgtgctgctc gcagctgctg
     2821 atgaatcaca tacttcctcc attttcttcc actgattgac tgttataatt tccctaattt
     2881 ccaggtcaag gtgctgtgca ttgtggtaat agatgtgaca tgacgtcact tccaaaggac
     2941 caatgaacat gtctgaccaa tttcatataa tgtgaaaacg attttcatag gcagaataaa
     3001 taacatttaa attaaactgg gcatcagcgc aattcaattg gtttggtaat agcaagggaa
     3061 aatagaatga agtgatctcc aaaaaataag tactttttga ctgtaaataa aattgtaagg
     3121 agtaaaaagt actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa gtattgattt
     3181 ttaattgtac tcaagtaaag taaaaatccc caaaaataat acttaagtac agtaatcaag
     3241 taaaattact caagtacttt acacctctgg ttcttgaccc cctaccttca gcaagcccag
     3301 cagatccact agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
     3361 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     3421 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3481 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3541 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3601 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3721 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3781 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3961 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4201 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     4261 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4321 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4441 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4501 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4561 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4621 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4681 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4741 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4801 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4861 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4921 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4981 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5041 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5101 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5161 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5221 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5281 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5341 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5401 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5461 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5521 gcacatttcc ccgaaaagtg
//
