LOCUS       pDONR223_SCAF4_W        6240 bp ds-DNA     circular     16-DEC-2014
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Unknown type
COMMENT     SerialCloner_Comments=GateWay(tm) BP reaction between SCAF  as an
            Expression fragm ent and pDONR223	5005 as a Donor vector.
COMMENT     SerialCloner_Ends=0,0,,0,
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     terminator      complement(270..297)
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="rrnB_T2_terminator" -
                     complement(268..295) \ 
                     /label=rrnB_T2_terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1663..1664
                     /label=Ex9_A_PMA
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1216..1219
                     /label=Ex6_A_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1684..1685
                     /label=Ex4_A_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      complement(429..472)
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="rrnB_T1_terminator" -
                     complement(427..470) \ 
                     /label=rrnB_T1_terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1666..1667
                     /label=Ex4_A_gRNA(1)
                     /ApEinfo_label=Ex4_A_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1249..1250
                     /label=Ex6_B_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1696..1697
                     /label=Ex9_B_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    524..546
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_pUC_fwd_primer" - 522..544 \
                     
                     /label=M13_pUC_fwd_primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1694..1694
                     /label=Ex9_B_gRNA(1)
                     /ApEinfo_label=Ex9_B_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1252..1253
                     /label=Ex6_B_gRNA(1)
                     /ApEinfo_label=Ex6_B_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1699..1700
                     /label=Ex9_B_gRNA(2)
                     /ApEinfo_label=Ex9_B_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        539..555
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_forward20_primer" - 537..553
                     \ 
                     /label=M13_forward20_primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1713..1715
                     /label=Ex9_B_PAm
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1255..1256
                     /label=Ex6_B_gRNA(2)
                     /ApEinfo_label=Ex6_B_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1702..1703
                     /label=Ex9_B_gRNA(3)
                     /ApEinfo_label=Ex9_B_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(571..654)
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="attL1" - complement(569..652) \ 
                     /label=attL1
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1201..1202
                     /label=Ex6_A_gRNA(1)
                     /ApEinfo_label=Ex6_A_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1258..1259
                     /label=Ex6_B_gRNA(3)
                     /ApEinfo_label=Ex6_B_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1705..1706
                     /label=Ex9_B_gRNA(4)
                     /ApEinfo_label=Ex9_B_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4133..4220
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="attL2" -
                     translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     -
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     -
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     -
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     - A*" - 2896..2983 \ 
                     /label=attL2
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1198..1199
                     /label=Ex6_A_PAM
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1261..1262
                     /label=Ex6_B_gRNA(4)
                     /ApEinfo_label=Ex6_B_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1708..1712
                     /label=Ex9_B_gRNA(5)
                     /ApEinfo_label=Ex9_B_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(4238..4256)
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="T7_promoter" -
                     translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     -
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     -
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     -
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     - A*" - complement(3001..3019) \ 
                     /label=T7_promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1246..1247
                     /label=Ex6_B_gRNA(5)
                     /ApEinfo_label=Ex6_B_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1264..1265
                     /label=Ex6_B_gRNA(6)
                     /ApEinfo_label=Ex6_B_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(4257..4275)
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_reverse_primer" -
                     translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     -
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     -
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     -
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     - A*" - complement(3020..3038) \ 
                     /label=M13_reverse_primer
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1267..1268
                     /label=Ex6_B_PAM
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1669..1670
                     /label=Ex4_A_gRNA(2)
                     /ApEinfo_label=Ex4_A_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            4489..5499
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="spect" - gene="spect" -
                     translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     -
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     -
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     -
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     - A*" - 3252..4262 \ 
                     /label=spect_spect
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1204..1205
                     /label=Ex6_A_gRNA(2)
                     /ApEinfo_label=Ex6_A_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1672..1673
                     /label=Ex4_A_gRNA(3)
                     /ApEinfo_label=Ex4_A_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             4489..5499
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="ORF frame 3" -
                     translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     -
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     -
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     -
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     -
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     -
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     - GK*" - 3252..4262 \ 
                     /label=ORF frame 3
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1207..1208
                     /label=Ex6_A_gRNA(3)
                     /ApEinfo_label=Ex6_A_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1675..1676
                     /label=Ex4_A_gRNA(4)
                     /ApEinfo_label=Ex4_A_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      5576..6195
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="pBR322_origin" -
                     translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA
                     -
                     RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL
                     -
                     AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE
                     -
                     VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL
                     -
                     VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA
                     -
                     PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV
                     - GK*" - 4339..4958 \  653..4136
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="Fragment inserted from SCAF  into
                     pDONR2235005 [BP] reaction \ 
                     /label=Insert from SCAF 
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1210..1211
                     /label=Ex6_A_gRNA(4)
                     /ApEinfo_label=Ex6_A_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1678..1679
                     /label=Ex4_A_gRNA(5)
                     /ApEinfo_label=Ex4_A_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(676..1060,1725..4119)
                     /label=SCAF4 ORF WT
                     /ApEinfo_fwdcolor=#80ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1213..1214
                     /label=Ex6_A_gRNA(5)
                     /ApEinfo_label=Ex6_A_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1681..1682
                     /label=Ex4_A_gRNA(6)
                     /ApEinfo_label=Ex4_A_gRNA
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT
       61 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA
      121 GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG
      181 CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TACGCGTACC
      241 GCTAGCCAGG AAGAGTTTGT AGAAACGCAA AAAGGCCATC CGTCAGGATG GCCTTCTGCT
      301 TAGTTTGATG CCTGGCAGTT TATGGCGGGC GTCCTGCCCG CCACCCTCCG GGCCGTTGCT
      361 TCACAACGTT CAAATCCGCT CCCGGCGGAT TTGTCCTACT CAGGAGAGCG TTCACCGACA
      421 AACAACAGAT AAAACGAAAG GCCCAGTCTT CCGACTGAGC CTTTCGTTTT ATTTGATGCC
      481 TGGCAGTTCC CTACTCTCGC GTTAACGCTA GCATGGATGT TTTCCCAGTC ACGACGTTGT
      541 AAAACGACGG CCAGTCTTAA GCTCGGGCCC CAAATAATGA TTTTATTTTG ACTGATAGTG
      601 ACCTGTTCGT TGCAACAAAT TGATGAGCAA TGCTTTTTTA TAATGCCAAC TTTGTACAAA
      661 AAAGCAGGCT TCACCatgga cgccgtcaac gccttcaacc aggagctctt ttcgcttatg
      721 gatatgaaac ctcccatctc tagagccaag atgattctca tcactaaagc tgctattaaa
      781 gctattaagc tttataagca tgtagttcaa atagtagaaa agttcatcaa aaagtgtaaa
      841 ccagaataca aggttccggg attatatgta attgactcaa ttgtgcgaca gtctcgtcat
      901 cagtttggaa ctgataaaga tgtttttggg ccaagattct ctaaaaacat aactgccaca
      961 ttccaatatt tatatctttg tccatctgaa gataagagta aaatagttcg tgtgctgaac
     1021 ctttggcaaa aaaatggagt gttcaaaatt gaaattattc aacctctttt ggacatggca
     1081 gcgggaacca gtaatgcagc cccagtagca gaaaatgtta ccaataatga aggctcacct
     1141 ccacctccag taaaagtttc ttctgaacct cccacacaag ccactccaaa ctccgtgcct
     1201 gcagttcctc aattacccag ctctgatgct tttgctgctg tggctcaact cttccaaact
     1261 acacagggcc aacagcttca gcagatcctt cagacttttc aacagcctcc aaaaccacag
     1321 tctcctgccc ttgacaatgc tgtgatggct caggttcagg ctatcacagc tcagttaaag
     1381 acaactccta cacaaccatc tgaacaaaaa gctgctttcc ccccacctga acagaaaact
     1441 gcatttgaca agaagttgct tgatagattt gactatgatg atgagccaga agctgtggaa
     1501 gaatcaaaga aagaggatac cactgccgtc accacgacag cacctgctgc cgcagtaccc
     1561 cctgcaccca ccgccaccgt gcctgctgct gctgcacccg ctgctgcctc tcctcctcct
     1621 ccacaggcac catttggctt tcctggagat ggcatgcagc agcctgctta tacgcaacac
     1681 cagaatatgg atcaattcca acctcgtatg atgggaatac aacaggatcc aatgcaccat
     1741 caggttccac ttcctcctaa tggacaaatg ccaggatttg gacttcttcc tacacctcca
     1801 tttcctccca tggctcagcc tgtgattcct ccaactccac cagtgcagca gcctttccaa
     1861 gcttcttttc aggcacaaaa tgaaccactt acacagaagc cgcatcagca ggaaatggaa
     1921 gtagaacaac cttgtattca agaggttaag cgacatatgt ctgataacag aaagtcaaga
     1981 tctaggtcag catccaggtc accaaaaagg aggcgatcta gatctggttc tagatctcga
     2041 aggtctcggc atcgacgttc tcgatctcgg tccagggata gacgccgaca ttctccccga
     2101 tctcgatctc aagaaagacg ggatcgagaa aaagagagag aacgtcgaca aaaaggcctc
     2161 cctcaagtga aaccggaaac tgcaagtgtt tgcagtacta ccctctgggt ggggcagctg
     2221 gacaaaagaa ctactcagca ggatgttgcc agtctcttgg aagagtttgg tccaattgaa
     2281 tcaattaata tgattcctcc caggggttgt gcctatattg ttatggttca taggcaagat
     2341 gcctatcgtg ccctgcagaa actgagccga ggaaactata aagtgaacca gaaatccata
     2401 aagattgcct gggccttaaa caaaggaata aaggcagatt ataagcagta ttgggatgta
     2461 gaacttggtg ttacttatat tccatgggac aaagtcaagc ctgaggaact ggagagtttt
     2521 tgtgaaggag gaatgttgga cagtgacaca cttaacccag attggaaagg aattcctaag
     2581 aagcctgaaa atgaagttgc tcaaaatgga ggtgctgaaa cctcacacac agaaccagta
     2641 tcacccatac ctaaaccatt acctgtgcct gtccctccta ttcctgttcc tgcacctata
     2701 acagtgccac ctccacaggt cccaccacat caaccgggtc cacctgtagt tggtgctctc
     2761 cagccgcctg ctttcacgcc tcctctggga ataccgcctc caggctttgg tcctggtgtt
     2821 cctcctcccc ctcctcctcc accatttttg cgcccaggat tcaacccaat gcatttacca
     2881 ccaggttttc tgcctcctgg acccccacct cctataactc caccagtatc cattcctcct
     2941 cctcacactc caccaataag catcccaaac tctactatcg ctggtataaa tgaagacact
     3001 acaaaagact tatctattgg aaatcccatt ccaacagtgg tgtctggggc tagaggaaac
     3061 gccgagtctg gtgacagcgt gaaaatgtat ggctctgccg tgccacctgc tgcacccacg
     3121 aatctgccca cccctcctgt aacccagcct gtttcacttc ttggcactca aggagttgcc
     3181 cctggtcctg taattggact tcaggcacca tctactggtc ttcttggcgc ccggcccggt
     3241 ctcatcccac tccagcgccc tccaggaatg cccccacctc acttacagcg gttccctttg
     3301 atgccgcccc gtcccatgcc accgcacatg atgcacagag gcccaccgcc aggaccaggg
     3361 ggctttgcga tgcctccacc tcatggaatg aaaggtccct tcccaccgca tggccccttt
     3421 gttaggcctg gtggaatgcc agggctcggg ggcccagggc caggcccagg gggtcctgaa
     3481 gacagagacg gaaggcaaca gccgccgcag cagccacagc agcagccaca gccgcaggcg
     3541 ccccagcaac cacagcagca gcagcagcag cagccaccac catcacaaca gcctccacca
     3601 acacagcagc agccacagca gtttagaaat gataacaggc agcagttcaa ttcaggtaga
     3661 gaccaagaaa ggtttggaag aagatctttt ggaaataggg tggaaaatga ccgggaacgg
     3721 tatgggaacc gtaatgatga tagagataat agtaaccgtg acaggagaga gtggggaagg
     3781 aggagccctg accgggacag gcacagagac ttggaagaga gaaatagacg ctctagtggg
     3841 catcgagaca gagagagaga ttctagagat agagagtctc gtagagagaa ggaagaagcc
     3901 cgaggaaagg aaaagcctga ggtgacagac agggcaggtg gtaacaaaac cgttgaacct
     3961 cccattagcc aagtgggaaa tgtagacact gcttcagaac ttgagaaggg ggtgtctgag
     4021 gctgcagtcc taaagccttc tgaagagtta cctgctgagg ctacctcatc cgttgaaccc
     4081 gaaaaggatt ctggctcagc agcagaggct cctcgttagA ACCCAGCTTT CTTGTACAAA
     4141 GTTGGCATTA TAAGAAAGCA TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT
     4201 CAAAATAAAA TCATTATTTG CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG
     4261 GTCATAGCTG TTTCCTGGCA GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA
     4321 CAAGATAAAA ATATATCATC ATGCCTCCTC TAGACCAGCC AGGACAGAAA TGCCTCGACT
     4381 TCGCTGCTGC CCAAGGTTGC CGGGTGACGC ACACCGTGGA AACGGATGAA GGCACGAACC
     4441 CAGTGGACAT AAGCCTGTTC GGTTCGTAAG CTGTAATGCA AGTAGCGTAT GCGCTCACGC
     4501 AACTGGTCCA GAACCTTGAC CGAACGCAGC GGTGGTAACG GCGCAGTGGC GGTTTTCATG
     4561 GCTTGTTATG ACTGTTTTTT TGGGGTACAG TCTATGCCTC GGGCATCCAA GCAGCAAGCG
     4621 CGTTACGCCG TGGGTCGATG TTTGATGTTA TGGAGCAGCA ACGATGTTAC GCAGCAGGGC
     4681 AGTCGCCCTA AAACAAAGTT AAACATCATG AGGGAAGCGG TGATCGCCGA AGTATCGACT
     4741 CAACTATCAG AGGTAGTTGG CGTCATCGAG CGCCATCTCG AACCGACGTT GCTGGCCGTA
     4801 CATTTGTACG GCTCCGCAGT GGATGGCGGC CTGAAGCCAC ACAGTGATAT TGATTTGCTG
     4861 GTTACGGTGA CCGTAAGGCT TGATGAAACA ACGCGGCGAG CTTTGATCAA CGACCTTTTG
     4921 GAAACTTCGG CTTCCCCTGG AGAGAGCGAG ATTCTCCGCG CTGTAGAAGT CACCATTGTT
     4981 GTGCACGACG ACATCATTCC GTGGCGTTAT CCAGCTAAGC GCGAACTGCA ATTTGGAGAA
     5041 TGGCAGCGCA ATGACATTCT TGCAGGTATC TTCGAGCCAG CCACGATCGA CATTGATCTG
     5101 GCTATCTTGC TGACAAAAGC AAGAGAACAT AGCGTTGCCT TGGTAGGTCC AGCGGCGGAG
     5161 GAACTCTTTG ATCCGGTTCC TGAACAGGAT CTATTTGAGG CGCTAAATGA AACCTTAACG
     5221 CTATGGAACT CGCCGCCCGA CTGGGCTGGC GATGAGCGAA ATGTAGTGCT TACGTTGTCC
     5281 CGCATTTGGT ACAGCGCAGT AACCGGCAAA ATCGCGCCGA AGGATGTCGC TGCCGACTGG
     5341 GCAATGGAGC GCCTGCCGGC CCAGTATCAG CCCGTCATAC TTGAAGCTAG ACAGGCTTAT
     5401 CTTGGACAAG AAGAAGATCG CTTGGCCTCG CGCGCAGATC AGTTGGAAGA ATTTGTCCAC
     5461 TACGTGAAAG GCGAGATCAC CAAGGTAGTC GGCAAATAAC CCTCGAGCCA CCCATGACCA
     5521 AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC
     5581 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA
     5641 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG
     5701 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA
     5761 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA
     5821 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG
     5881 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG
     5941 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG
     6001 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG
     6061 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC
     6121 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA
     6181 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG
//
