LOCUS Exported 6516 bp ds-DNA circular SYN 24-JAN-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6516) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 24, 2019 from SnapGene 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6516 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..5251 /label=Untitled /note="���������������������������������������������������� ������������" misc_feature 1..175 /label=1.65 for /note="1.65 for" primer_bind complement(155..172) /label=r.AAV backbone seq.01.67 /note="r.AAV backbone seq.01.67" misc_feature 176..708 /label=1.59rev seq /note="1.59rev seq" primer_bind complement(285..306) /label=r.AAV backbone seq.01.66 /note="r.AAV backbone seq.01.66" primer_bind 709..1294 /label=1.19 1.20 FOR SEQ /note="1.19 1.20 FOR SEQ" primer_bind complement(855..877) /label=r.AAV backbone seq.01.59 /note="r.AAV backbone seq.01.59" misc_feature complement(1104..1818) /label=3.112r sequence /note="3.112r sequence" misc_feature 1295..2726 /label=sequenced /note="sequenced" misc_feature 1329..2190 /label=1.17r seq /note="1.17r seq" misc_feature complement(1671..2391) /label=1.53r (syn rev) seq /note="1.53r (syn rev) seq" misc_feature 1722..2669 /label=Mouse synapsin I gene, exon 1 /note="Mouse synapsin I gene, exon 1" primer_bind complement(1845..1868) /label=r.iCRE seq.03.112 /note="r.iCRE seq.03.112" misc_feature complement(2249..3129) /label=3.104r and 3.105r seq /note="3.104r and 3.105r seq" gene 2727..3785 /label=insert: Recombinase iCRE CDS 2763..3755 /codon_start=1 /label=Recombinase_CRE /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETG" primer_bind 2916..2936 /label=f.iCRE seq.03.101 /note="f.iCRE seq.03.101" primer_bind complement(2916..2936) /label=r.iCRE seq.03.104 /note="r.iCRE seq.03.104" misc_feature 2990..4772 /label=sequenced /note="sequenced" primer_bind 3164..3187 /label=f.iCRE seq.03.102 /note="f.iCRE seq.03.102" primer_bind complement(3164..3187) /label=r.iCRE seq.03.105 /note="r.iCRE seq.03.105" primer_bind 3474..3491 /label=f.iCRE seq.03.103 /note="f.iCRE seq.03.103" misc_feature 3552..4273 /label=3.103f seq /note="3.103f seq" primer_bind 3795..3824 /label=HA-TAG /note="HA-TAG" misc_feature 3847..4435 /label=WPRE /note="WPRE" misc_feature 3918..3937 /label=WPRE rev /note="WPRE rev" misc_feature 3983..4003 /label=Mao 115r /note="Mao 115r" primer_bind 4094..4112 /label=f.wpre seq.01.50 /note="f.wpre seq.01.50" misc_feature 4153..4752 /label=1.50f seq /note="1.50f seq" primer_bind 4461..4484 /label=f.ITR for 3.01.15 /note="f.ITR for 3.01.15" primer_bind 4601..4621 /label=f.ITR for 4.01.16 /note="f.ITR for 4.01.16" misc_feature complement(4746..4861) /label=1.13r seq /note="1.13r seq" primer_bind 4749..5251 /label=1.16f sequence /note="1.16f sequence" misc_feature complement(4897..4917) /label=r.ITR for 1.01.13 /note="r.ITR for 1.01.13" primer_bind 5078..5101 /label=f.AAV backbone seq.01.60 /note="f.AAV backbone seq.01.60" primer_bind 5185..5206 /label=f.AAV backbone seq.01.61 /note="f.AAV backbone seq.01.61" misc_feature 5252..5825 /label=1.60f 1.61f seq /note="1.60f 1.61f seq" primer_bind 5491..5507 /label=f.AAV backbone seq.01.63 /note="f.AAV backbone seq.01.63" gene complement(5631..6491) /label=ampicillin /note="ampicillin" primer_bind 5798..5818 /label=f.AAV backbone seq.01.64 /note="f.AAV backbone seq.01.64" misc_feature 5826..6412 /label=1.63f 1.64f seq /note="1.63f 1.64f seq" misc_feature 6413..6516 /label=1.66r, 1.67r /note="1.66r, 1.67r" primer_bind 6464..6481 /label=f.AAV backbone seq.01.65 /note="f.AAV backbone seq.01.65" ORIGIN 1 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 61 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 121 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg 181 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 241 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 301 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 361 aaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 421 ttttttaacc aatagaccga aatcggcaaa atcccttata aatcaaaaga atagcccgag 481 atagagttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 541 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 601 aaatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 661 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 721 gcgaaaggag cgggcgctaa ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 781 acacccgccg cgcttaatgc gccgctacag ggcgcgtact atggttgctt tgacgtatgc 841 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc ccctgcaggc 901 agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct 961 ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca 1021 ctaggggttc ctgcggccgc ggtaccttaa ttaagggatg taattacgtc cctaacccgc 1081 tagggggcag cagcgagccg aacggggctc cgctccggtc cggcgctaac cccgcatccc 1141 cgagggggga tgtaattacg tccctaaccc gctagggggc agcagcgagc cgaacggggc 1201 tccgctccgg tccggcgcta accccgcatc cccgaggggg gatgtaatta cgtccctaac 1261 ccgctagggg gcagcagcga gccgaacggg gctccgctcc ggtccggcgc taaccccgca 1321 tccccgaggg tcgactatta atagtaatca attacggggt cattagttca tagcccatat 1381 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 1441 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 1501 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 1561 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 1621 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 1681 atcgctatta ccattcagaa ccacggacag caccgaattc aatgtgtaat gtgtgctcaa 1741 tatgctcatg tgtgttaccc tgagcttctg tgtctacata tatacctgga tgcctgtgtg 1801 ttctgtgatg tacatatata ttctgtcttt ccttcctttt ctatttgtgt tattccatgt 1861 gttctttcag attctcacca ccaagggcaa ggatatgtta actacccaag tgtccacctc 1921 cgcctgtctg gtgatgttta cgccaccccc gtgctctttt ctttgcccga cagagttgtt 1981 ataggagatg tctccccggg aacactgcag gaaggagaat ttctacattt atgttcccct 2041 ctgagtgtgc ttctatcccc aaaatgcctt caaaggtgaa aatcaacact ggaaacccaa 2101 gtatctggga agggcaagag tgtgtaagtg caagttagcc taaggaatag gaagaggttg 2161 gtaaacaggg taggatcgtg ggagggagtt tcgttactac aggtccggac cctcaggaca 2221 agaaccccac ccccactccc caaattgcgc atcccccgcc cccatcagag ggggagggga 2281 agaggttgcg gcgcggcgca tgcgcactgt cggattcagc accgcggtca gagccttcgc 2341 ctccgctgcc ggcgcgcacc accacctccc cagcaccaaa ggctgactga cgtcactcac 2401 tagccctccc caaactcccc ttcctcgccg ccttggtcgc gtccatgctg ccgtgagtcc 2461 agtcggaccg caccacgaga ggtgcaagat agggaggtgc gggcgcgacc atacgctctg 2521 cggcggcaga gcctcggcgc tgcctcagtc tgcagcgggc agcagaggag tcgcgtcgtg 2581 ccagagagca ccgccgtgct cctgagcccc tcgcgctccg cccccgcggc ccacagaccc 2641 actgcccctt ggatcccctg ccccacgcca gagcctctgc taaccatgtt catgccttct 2701 tctttttcct acaggagctc gccaccgcca ccatggtgcc caagaagaag aggaaagtct 2761 ccaacctgct gactgtgcac caaaacctgc ctgccctccc tgtggatgcc acctctgatg 2821 aagtcaggaa gaacctgatg gacatgttca gggacaggca ggccttctct gaacacacct 2881 ggaagatgct cctgtctgtg tgcagatcct gggctgcctg gtgcaagctg aacaacagga 2941 aatggttccc tgctgaacct gaggatgtga gggactacct cctgtacctg caagccagag 3001 gcctggctgt gaagaccatc caacagcacc tgggccagct caacatgctg cacaggagat 3061 ctggcctgcc tcgcccttct gactccaatg ctgtgtccct ggtgatgagg agaatcagaa 3121 aggagaatgt ggatgctggg gagagagcca agcaggccct ggcctttgaa cgcactgact 3181 ttgaccaagt cagatccctg atggagaact ctgacagatg ccaggacatc aggaacctgg 3241 ccttcctggg cattgcctac aacaccctgc tgcgcattgc cgaaattgcc agaatcagag 3301 tgaaggacat ctcccgcacc gatggtggga gaatgctgat ccacattggc aggaccaaga 3361 ccctggtgtc cacagctggt gtggagaagg ccctgtccct gggggttacc aagctggtgg 3421 agagatggat ctctgtgtct ggtgtggctg atgaccccaa caactacctg ttctgccggg 3481 tcagaaagaa tggtgtggct gccccttctg ccacctccca actgtccacc cgggccctgg 3541 aagggatctt tgaggccacc caccgcctga tctatggtgc caaggatgac tctgggcaga 3601 gatacctggc ctggtctggc cactctgcca gagtgggtgc tgccagggac atggccaggg 3661 ctggtgtgtc catccctgaa atcatgcagg ctggtggctg gaccaatgtg aacattgtga 3721 tgaactacat cagaaacctg gactctgaga ctggggccat ggtgaggctg ctcgaggatg 3781 gggacagcgg cggataccct tacgacgtgc ctgactacgc ctgactgcag aacggccgtt 3841 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 3901 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 3961 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 4021 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 4081 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 4141 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 4201 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg 4261 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 4321 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 4381 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga 4441 ttgcggccgt cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg 4501 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt 4561 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag 4621 ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tagcggccgc 4681 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 4741 ccgggcgacc aaaggtcgcc cgacgcccgg gcggcctcag tgagcgagcg agcgcgcagc 4801 tgcctgcagg acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 4861 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 4921 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 4981 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 5041 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 5101 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 5161 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 5221 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 5281 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 5341 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 5401 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 5461 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 5521 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 5581 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 5641 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 5701 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 5761 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 5821 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 5881 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 5941 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 6001 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 6061 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 6121 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 6181 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 6241 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 6301 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 6361 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 6421 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 6481 tgaatactca tactcttcct ttttcaatat tattga //