LOCUS       Exported                7171 bp ds-DNA     circular SYN 25-JAN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Pnhx2_lmp-1_CemOrange2.nucl Assembly
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 25, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      24..2004
                     /note="Pnhx-2"
     misc_feature    complement(1707..2750)
                     /label=Pnhx-2lmp-1CemOrange2-Lmp-1_Seq_1R
                     /note="Pnhx-2lmp-1CemOrange2-Lmp-1_Seq_1R"
     misc_feature    1928..2889
                     /label=Pnhx-2lmp-1CemOrange2-Pnhx-2_seq_4F
                     /note="Pnhx-2lmp-1CemOrange2-Pnhx-2_seq_4F"
     misc_feature    complement(2027..3066)
                     /label=Pnhx-2lmp-1CemOrange2-CemOrange2_Seq_R
                     /note="Pnhx-2lmp-1CemOrange2-CemOrange2_Seq_R"
     misc_feature    2089..3017
                     /label=C-terminal Fusion Multiple Cloning Site [Split]
                     /note="C-terminal Fusion Multiple Cloning Site [Split]"
     gene            2090..3923
                     /label=mOrange2 [Split]
                     /note="mOrange2 [Split]"
     misc_feature    2095..2991
                     /label=1 to 897 of lmp-1 gDNA
                     /note="1 to 897 of lmp-1 gDNA"
     gene            2095..2991
                     /label=lmp-1 HiFi assembly
                     /note="lmp-1 HiFi assembly"
     misc_feature    2340..3288
                     /label=Pnhx-2lmp-1CemOrange2-Lmp-1_Seq_1F
                     /note="Pnhx-2lmp-1CemOrange2-Lmp-1_Seq_1F"
     misc_feature    3889..3924
                     /label=N terminal Multiple Cloning Site
                     /note="N terminal Multiple Cloning Site"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgca gctttgtggc gaccaccacg tgtctactga
       61 taaattaaca gattgtacta ctttgcatgt aatgtgctag aggacggttc tcttatcatc
      121 acaggcactg acgacactgc caaagtttta caagctaatt tattagcgct cttcggccgc
      181 aatggcacga tttgaattgg tttattgatt ccagagacta gtttcagttt ttgataagaa
      241 tgaattatta ttcattttct agtttatgaa tatatatgta actagggaag cctatttatg
      301 atttttctaa ttttttaaaa ttgttatttt agagaggtgt gaaattaaaa tgatatatat
      361 tttcgagtac tttaaaaaat aaaaacggta catcctatat taaatttttg attttagatc
      421 cttccaagtt attgacaagt attaactgca cttctcataa aaatttttca aatagatcta
      481 tttagtctgg aataggaata cttttggcaa ttttcttgag aatcatagaa gacgaatttc
      541 tgaactgctc tttgtgaagc cacgcccata tattggcatt tttgtatcca attttttgta
      601 gagtacctga atcattatca ctacatctgc taaacaatat atggaagttc tatgagaggt
      661 gaaaggtaaa tcatataaca actgtaataa catagaaatg attgtagtat atttccaatt
      721 aggctgagtt gatcttattg aagagtttga atcatagcaa ttttttcctc ttttagtatt
      781 tttcaaaaca aattttcaca atgttagatg tagtatttag cattaaggca tctgtaatct
      841 cccagaaatg ttttgggaat aaaacccttt gactctttgg tccttttttg ctccaaataa
      901 tatctgattt taacccgttc tgatctactt tttacctttc gatcgtcaca tccgcgtaca
      961 cgtttctctt gctctcaatt gcttatcttt atggcctttt cccccacttt ttgtcgacta
     1021 tcatgcggac gtggcttcct tgctccctcc cggttttttt gaaaacactt gtgaatttat
     1081 tttttgtggt caaaattacg aaacatttga attctcaatc tatttataga ccgggtgtgg
     1141 tatgttatgc catgtttttg tcattgccaa atgaacatcg atattttaat tttctgaact
     1201 taccgtaccc cctgcgttac tattttattt ttaatttgta taatttcatt gctataattt
     1261 gttgtccttg aaacattgaa gatatcgaag aaataatgac aaaaatcaga tagaacaatt
     1321 tttcctactc atcaactcac atttttctat tttctcttgt gcgaaaaaaa tagtatattg
     1381 ggaatattta tagtattgca acttttctct tagttcgcag agttgcaata ctactagatc
     1441 cctaatagag gaaatatggt gaccagcttt ttgcaattgg aaagttggga aaaaagtata
     1501 tatattaata gacttacact cttgtagttc gtagaatttt tcaggaaatc tgacacgcaa
     1561 gactttttca aaaagttttt ttttttaatt tggcaatctg ccaaaacgtt tactgaacat
     1621 tttgaaaaca tctaccagta cctttaacac aacgaaaaat tgttctaagc aatattaact
     1681 gtgataattg atgttaagac ctgaaattta atcacaaatt tcacctggaa atgacagtaa
     1741 tcggcttcta aatactgtcg acacaagtgc catacctaag attccaatcc tattaccatg
     1801 tttcccaatt tcattctctt tcgtatctct atttctcttc tttattatca attacttttt
     1861 atcagttctt cgtttgcttt aacatcaaga gcacatagcg ctctctcgtc tctctctcat
     1921 gctctttttg aacttttaca aaaaactatt tccggttgtt ttgattcttg gaatttgaaa
     1981 taattttcag tgattaaatc tagaggatcc ccgggattgg ccaaaggacc caaaggtatg
     2041 tttcgaatga tactaacata acatagaaca ttttcaggag gacccttggc tagcaaaaat
     2101 gttgaaatcg tttgtcatct tgtttggtaa gtgaaatact tcctacgcag aaatacaata
     2161 gcaatcaatc aattttcagc cttcctggcc tccgcatccg ccaccgcttc gcattactat
     2221 gtaaccaaca acaacaccgg actcacgtgc atcatcctcg acggagactt ccagttcaat
     2281 ttggtcttca acgagaagaa caccaccgag aagttcacgg tcacattcaa cgagacagtc
     2341 agtgtcgaag gagattgcaa cggagtccgc aacaatcaat cggttcagac gttgaatgtg
     2401 agtttttgaa taacaacttt agggcaattt ataacttgaa aaaatttcca gattaagttc
     2461 aaccctgaag gacaatctgc ccgttacgcc aaagagtggg agctcgatat cgtgttcgga
     2521 tcatctagca acgtcgcctt tgagatcatt gactacaccc tcaccactca gagaacggat
     2581 cttgtcccat actttggaaa gtttgtcaga gacgccaatg ctgccggaga tgttacagca
     2641 actcagacca acgcttacaa ggtaatacca tttctgctat agcatttggg gtcttgttac
     2701 cactcactat ttcttgccta actcaaaaat tttcagtgct ccaccgccaa gcttggactc
     2761 gtcggaggat ctaccattga catcaagaca tccaacgtga ttgcatttgc acagatgaac
     2821 ggcaccgttt tcccaactga ccaagtctac gaggtgtgct atttggatgc tcgcaccaac
     2881 gaagttgtcc caatcgtcgt cggagcctgc ctcgcggctc tcgtcgtggt tgtgctcgtc
     2941 ggatacctta tcggacgtgc tcgtgcaaag agacaaggat atgccagcgt cctcgagagg
     3001 cctggtacca tgggcggaaa aatggtctcc aagggagagg agaacaacat ggccatcatc
     3061 aaggagttca tgcgtttcaa ggtccgtatg gagggatccg tcaacggaca cgagttcgag
     3121 atcgagggag agggagaggg acgtccatac gagggattcc aaaccgccaa gctcaaggtc
     3181 accaaggtaa gtttaaacat atatatacta actaaccctg attatttaaa ttttcaggga
     3241 ggaccactcc cattcgcctg ggacatcctc tccccacact tcacctacgg atccaaggcc
     3301 tacgtcaagc acccagccga catcccagac tacttcaagc tctccttccc agagggattc
     3361 aagtgggagc gtgtcatgaa ctacgaggac ggaggagtcg tcaccgtcac ccaagactcc
     3421 tccctccaag acggagagtt catctacaag gtaagtttaa acagttcggt actaactaac
     3481 catacatatt taaattttca ggtcaagctc cgtggaacca acttcccatc cgacggacca
     3541 gtcatgcaaa agaagaccat gggatgggag gcctcctccg agcgtatgta cccagaggac
     3601 ggagccctca agggaaagat caagatgcgt ctcaagctca aggtaagttt aaacatgatt
     3661 ttactaacta actaatctga tttaaatttt caggacggag gacactacac ctccgaggtc
     3721 aagaccacct acaaggccaa gaagccagtc caactcccag gagcctacat cgtcgacatc
     3781 aagctcgaca tcacctccca caacgaggac tacaccatcg tcgagcaata cgagcgtgcc
     3841 gagggacgtc actccaccgg aggaatggac gaactctaca agcttaacgg tcctaggaga
     3901 tctgaattcg gcgcctaaga gctccgcatc ggccgctgtc atcagatcgc catctcgcgc
     3961 ccgtgcctct gacttctaag tccaattact cttcaacatc cctacatgct ctttctccct
     4021 gtgctcccac cccctatttt tgttattatc aaaaaaactt cttcttaatt tctttgtttt
     4081 ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa tagaattaat
     4141 tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata attctatccc
     4201 aaaatctaca caatgttctg tgtacacttc ttatgttttt tttacttctg ataaattttt
     4261 tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt
     4321 tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca aatcatgctc
     4381 atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag tttatgaaat
     4441 taattttcct gcttttgctt tttgggggtt tcccctattg tttgtcaaga gtttcgagga
     4501 cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa agatcggaag
     4561 aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg aaagtggagt
     4621 agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac caaacataac
     4681 tgtttcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg
     4741 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     4801 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     4861 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     4921 acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta
     4981 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     5041 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5101 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5161 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5221 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5281 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5341 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     5401 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5461 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5521 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5581 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5641 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5701 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5761 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5821 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5881 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5941 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6001 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6061 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6121 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6181 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6241 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6301 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     6361 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6421 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6481 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6541 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     6601 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6661 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6721 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6781 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6841 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6901 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     6961 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7021 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7081 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7141 agcggataac aatttcacac aggaaacagc t
//
