LOCUS       Exported                7314 bp ds-DNA     circular SYN 25-JAN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Pnhx2_pisy-1_CemOrange2.nucl Assembly
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 25, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     gene            0..4048
                     /label=pisy-1 gDNA hifi assembly
                     /note="pisy-1 gDNA hifi assembly"
     regulatory      24..2004
                     /note="Pnhx-2"
     misc_feature    2089..2120
                     /label=C-terminal Fusion Multiple Cloning Site
                     /note="C-terminal Fusion Multiple Cloning Site"
     gene            2090..4066
                     /label=mOrange2 [Split]
                     /note="mOrange2 [Split]"
     misc_feature    2895..3823
                     /label=Pnhx-2CemOrange2pisy-1_3003-CemOrange2_2F_seq
                     /note="Pnhx-2CemOrange2pisy-1_3003-CemOrange2_2F_seq"
     misc_feature    2992..4067
                     /label=N terminal Multiple Cloning Site [Split]
                     /note="N terminal Multiple Cloning Site [Split]"
     misc_feature    3053..4020
                     /label=Pnhx-2CemOrange2pisy-1_3003-pisy-1_1F_seq
                     /note="Pnhx-2CemOrange2pisy-1_3003-pisy-1_1F_seq"
     misc_feature    3466..4416
                     /label=Pnhx-2CemOrange2pisy-1_3003-pisy-1_2F_seq
                     /note="Pnhx-2CemOrange2pisy-1_3003-pisy-1_2F_seq"
     misc_feature    3944..4904
                     /label=Pnhx-2CemOrange2pisy-1_3003-pisy-1_3F_seq
                     /note="Pnhx-2CemOrange2pisy-1_3003-pisy-1_3F_seq"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgca gctttgtggc gaccaccacg tgtctactga
       61 taaattaaca gattgtacta ctttgcatgt aatgtgctag aggacggttc tcttatcatc
      121 acaggcactg acgacactgc caaagtttta caagctaatt tattagcgct cttcggccgc
      181 aatggcacga tttgaattgg tttattgatt ccagagacta gtttcagttt ttgataagaa
      241 tgaattatta ttcattttct agtttatgaa tatatatgta actagggaag cctatttatg
      301 atttttctaa ttttttaaaa ttgttatttt agagaggtgt gaaattaaaa tgatatatat
      361 tttcgagtac tttaaaaaat aaaaacggta catcctatat taaatttttg attttagatc
      421 cttccaagtt attgacaagt attaactgca cttctcataa aaatttttca aatagatcta
      481 tttagtctgg aataggaata cttttggcaa ttttcttgag aatcatagaa gacgaatttc
      541 tgaactgctc tttgtgaagc cacgcccata tattggcatt tttgtatcca attttttgta
      601 gagtacctga atcattatca ctacatctgc taaacaatat atggaagttc tatgagaggt
      661 gaaaggtaaa tcatataaca actgtaataa catagaaatg attgtagtat atttccaatt
      721 aggctgagtt gatcttattg aagagtttga atcatagcaa ttttttcctc ttttagtatt
      781 tttcaaaaca aattttcaca atgttagatg tagtatttag cattaaggca tctgtaatct
      841 cccagaaatg ttttgggaat aaaacccttt gactctttgg tccttttttg ctccaaataa
      901 tatctgattt taacccgttc tgatctactt tttacctttc gatcgtcaca tccgcgtaca
      961 cgtttctctt gctctcaatt gcttatcttt atggcctttt cccccacttt ttgtcgacta
     1021 tcatgcggac gtggcttcct tgctccctcc cggttttttt gaaaacactt gtgaatttat
     1081 tttttgtggt caaaattacg aaacatttga attctcaatc tatttataga ccgggtgtgg
     1141 tatgttatgc catgtttttg tcattgccaa atgaacatcg atattttaat tttctgaact
     1201 taccgtaccc cctgcgttac tattttattt ttaatttgta taatttcatt gctataattt
     1261 gttgtccttg aaacattgaa gatatcgaag aaataatgac aaaaatcaga tagaacaatt
     1321 tttcctactc atcaactcac atttttctat tttctcttgt gcgaaaaaaa tagtatattg
     1381 ggaatattta tagtattgca acttttctct tagttcgcag agttgcaata ctactagatc
     1441 cctaatagag gaaatatggt gaccagcttt ttgcaattgg aaagttggga aaaaagtata
     1501 tatattaata gacttacact cttgtagttc gtagaatttt tcaggaaatc tgacacgcaa
     1561 gactttttca aaaagttttt ttttttaatt tggcaatctg ccaaaacgtt tactgaacat
     1621 tttgaaaaca tctaccagta cctttaacac aacgaaaaat tgttctaagc aatattaact
     1681 gtgataattg atgttaagac ctgaaattta atcacaaatt tcacctggaa atgacagtaa
     1741 tcggcttcta aatactgtcg acacaagtgc catacctaag attccaatcc tattaccatg
     1801 tttcccaatt tcattctctt tcgtatctct atttctcttc tttattatca attacttttt
     1861 atcagttctt cgtttgcttt aacatcaaga gcacatagcg ctctctcgtc tctctctcat
     1921 gctctttttg aacttttaca aaaaactatt tccggttgtt ttgattcttg gaatttgaaa
     1981 taattttcag tgattaaatc tagaggatcc ccgggattgg ccaaaggacc caaaggtatg
     2041 tttcgaatga tactaacata acatagaaca ttttcaggag gacccttggc tagcctcgag
     2101 aggcctggta ccatgggcgg aaaaatggtc tccaagggag aggagaacaa catggccatc
     2161 atcaaggagt tcatgcgttt caaggtccgt atggagggat ccgtcaacgg acacgagttc
     2221 gagatcgagg gagagggaga gggacgtcca tacgagggat tccaaaccgc caagctcaag
     2281 gtcaccaagg taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag
     2341 ggaggaccac tcccattcgc ctgggacatc ctctccccac acttcaccta cggatccaag
     2401 gcctacgtca agcacccagc cgacatccca gactacttca agctctcctt cccagaggga
     2461 ttcaagtggg agcgtgtcat gaactacgag gacggaggag tcgtcaccgt cacccaagac
     2521 tcctccctcc aagacggaga gttcatctac aaggtaagtt taaacagttc ggtactaact
     2581 aaccatacat atttaaattt tcaggtcaag ctccgtggaa ccaacttccc atccgacgga
     2641 ccagtcatgc aaaagaagac catgggatgg gaggcctcct ccgagcgtat gtacccagag
     2701 gacggagccc tcaagggaaa gatcaagatg cgtctcaagc tcaaggtaag tttaaacatg
     2761 attttactaa ctaactaatc tgatttaaat tttcaggacg gaggacacta cacctccgag
     2821 gtcaagacca cctacaaggc caagaagcca gtccaactcc caggagccta catcgtcgac
     2881 atcaagctcg acatcacctc ccacaacgag gactacacca tcgtcgagca atacgagcgt
     2941 gccgagggac gtcactccac cggaggaatg gacgaactct acaagcttaa cggtcctagg
     3001 agatctgaat tcgaaccgac agccgccgag aacaacaaca atgtgtggct attctacccg
     3061 aatctcatcg gttatggacg catagtactc gccattttgg ccatgtacta catgtcgtca
     3121 gcgccatggt gtgcaatgtt ctggtgagtt ttggcgagct ggaaatcgat ttttgcatca
     3181 aaaattagct aatatccatg tttactacta cttttcatct ctttttcact gaaatgtcga
     3241 gtatttttgc gatgcatggt aaagaaatca aggtgaccag gtttcttttc aattttcccc
     3301 cataattgaa gagttacaat gccatctgac aaggtttttc agaagaaaag tcctgaaaat
     3361 tagattttcg taattaaaat ttctcataat ttcagctatg cactcagcgc cggactcgac
     3421 gcgtttgacg gatgggcggc acggacctac aatcaatcga gccgatttgg agcaatgctc
     3481 gatcagctca ccgatcggta cgttaaaatt tctcaaatat gctggaaaat tcgagaaaaa
     3541 aattggattc ggtaaaattc tcatattttc agctgcgccc tgctggcctt ggtgatggcg
     3601 ctctgcaagt tctacccgga tcacttgttc ctgcttcaac tgtcggccgt aatcgacatc
     3661 gcatctcact ggcttcacct tcacgccact gatctctccg gtgccacgag ccacaagcag
     3721 tcttcgaatt ggcttctgaa cctctactac acggatcgat tgttcctcgg attcatgtgc
     3781 ggcggaaatg aggctttcta cattttgctc tacattcgcg cgttctgggc tggtaaatat
     3841 ttgatttatt tttgctataa tttattaatt cctctaattt tcaggaccac tgatcttcaa
     3901 cttccacttc atgtcgatct tcccactcat cgccttcccg attgccttcg tcaagtccgc
     3961 catctccgtc gtccatctgt acactgccgc cgagatggtc gttgctcacg atgtgaagct
     4021 ccgtgttgag gctgctcaga aggcccaatg acggcgccta agagctccgc atcggccgct
     4081 gtcatcagat cgccatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac
     4141 atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaaa
     4201 cttcttctta atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt
     4261 gtagattcaa aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc
     4321 cccattaata ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt
     4381 ttttttactt ctgataaatt ttttttgaaa catcatagaa aaaaccgcac acaaaatacc
     4441 ttatcatatg ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc
     4501 tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc
     4561 aatcaagtga aagtttatga aattaatttt cctgcttttg ctttttgggg gtttccccta
     4621 ttgtttgtca agagtttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag
     4681 cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga
     4741 agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac
     4801 attcccaata taccaaacat aactgtttcc tactagtcgg ccgtacgggc cctttcgtct
     4861 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     4921 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     4981 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     5041 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcggcc
     5101 ttaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5161 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5221 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5281 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5341 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5401 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5461 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5521 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5581 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5641 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5701 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5761 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5821 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5881 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5941 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6001 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6061 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6121 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6181 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6241 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6301 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6361 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6421 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     6481 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6541 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6601 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6661 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6721 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6781 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6841 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6901 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6961 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7021 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7081 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7141 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7201 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7261 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agct
//
