LOCUS       Exported                6343 bp ds-DNA     circular SYN 25-JAN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Pnhx2_mitotag_CemOrange2 fixed  Alignment
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 25, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    0..2196
                     /label=2113 to 2187 of Pnhx2_mitotag_CemOrange2
                     /note="2113 to 2187 of Pnhx2_mitotag_CemOrange2"
     regulatory      24..2004
                     /note="Pnhx-2"
     misc_feature    complement(1294..2238)
                     /label=Pnhx-2mitoCemOr2_5-CemOrange2_seq_R
                     /note="Pnhx-2mitoCemOr2_5-CemOrange2_seq_R"
     misc_feature    join(2089..6343,1..0)
                     /label=C-terminal Fusion Multiple Cloning Site [Split]
                     /note="C-terminal Fusion Multiple Cloning Site [Split]"
     gene            2113..3095
                     /label=mOrange2 [Split]
                     /note="mOrange2 [Split]"
     misc_feature    2125..2196
                     /label=4 to 75 of Mito Target Peptide na seq
                     /note="4 to 75 of Mito Target Peptide na seq"
     misc_feature    3061..3096
                     /label=N terminal Multiple Cloning Site
                     /note="N terminal Multiple Cloning Site"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgca gctttgtggc gaccaccacg tgtctactga
       61 taaattaaca gattgtacta ctttgcatgt aatgtgctag aggacggttc tcttatcatc
      121 acaggcactg acgacactgc caaagtttta caagctaatt tattagcgct cttcggccgc
      181 aatggcacga tttgaattgg tttattgatt ccagagacta gtttcagttt ttgataagaa
      241 tgaattatta ttcattttct agtttatgaa tatatatgta actagggaag cctatttatg
      301 atttttctaa ttttttaaaa ttgttatttt agagaggtgt gaaattaaaa tgatatatat
      361 tttcgagtac tttaaaaaat aaaaacggta catcctatat taaatttttg attttagatc
      421 cttccaagtt attgacaagt attaactgca cttctcataa aaatttttca aatagatcta
      481 tttagtctgg aataggaata cttttggcaa ttttcttgag aatcatagaa gacgaatttc
      541 tgaactgctc tttgtgaagc cacgcccata tattggcatt tttgtatcca attttttgta
      601 gagtacctga atcattatca ctacatctgc taaacaatat atggaagttc tatgagaggt
      661 gaaaggtaaa tcatataaca actgtaataa catagaaatg attgtagtat atttccaatt
      721 aggctgagtt gatcttattg aagagtttga atcatagcaa ttttttcctc ttttagtatt
      781 tttcaaaaca aattttcaca atgttagatg tagtatttag cattaaggca tctgtaatct
      841 cccagaaatg ttttgggaat aaaacccttt gactctttgg tccttttttg ctccaaataa
      901 tatctgattt taacccgttc tgatctactt tttacctttc gatcgtcaca tccgcgtaca
      961 cgtttctctt gctctcaatt gcttatcttt atggcctttt cccccacttt ttgtcgacta
     1021 tcatgcggac gtggcttcct tgctccctcc cggttttttt gaaaacactt gtgaatttat
     1081 tttttgtggt caaaattacg aaacatttga attctcaatc tatttataga ccgggtgtgg
     1141 tatgttatgc catgtttttg tcattgccaa atgaacatcg atattttaat tttctgaact
     1201 taccgtaccc cctgcgttac tattttattt ttaatttgta taatttcatt gctataattt
     1261 gttgtccttg aaacattgaa gatatcgaag aaataatgac aaaaatcaga tagaacaatt
     1321 tttcctactc atcaactcac atttttctat tttctcttgt gcgaaaaaaa tagtatattg
     1381 ggaatattta tagtattgca acttttctct tagttcgcag agttgcaata ctactagatc
     1441 cctaatagag gaaatatggt gaccagcttt ttgcaattgg aaagttggga aaaaagtata
     1501 tatattaata gacttacact cttgtagttc gtagaatttt tcaggaaatc tgacacgcaa
     1561 gactttttca aaaagttttt ttttttaatt tggcaatctg ccaaaacgtt tactgaacat
     1621 tttgaaaaca tctaccagta cctttaacac aacgaaaaat tgttctaagc aatattaact
     1681 gtgataattg atgttaagac ctgaaattta atcacaaatt tcacctggaa atgacagtaa
     1741 tcggcttcta aatactgtcg acacaagtgc catacctaag attccaatcc tattaccatg
     1801 tttcccaatt tcattctctt tcgtatctct atttctcttc tttattatca attacttttt
     1861 atcagttctt cgtttgcttt aacatcaaga gcacatagcg ctctctcgtc tctctctcat
     1921 gctctttttg aacttttaca aaaaactatt tccggttgtt ttgattcttg gaatttgaaa
     1981 taattttcag tgattaaatc tagaggatcc ccgggattgg ccaaaggacc caaaggtatg
     2041 tttcgaatga tactaacata acatagaaca ttttcaggag gacccttggc tagcctcgag
     2101 aggcctggta ccggcggaaa aatgctctcc ctccgtcaat ccatccgttt cttcaagcca
     2161 gccacccgta ccctctgctc ctcccgtacc ctcctcgtct ccaagggaga ggagaacaac
     2221 atggccatca tcaaggagtt catgcgtttc aaggtccgta tggagggatc cgtcaacgga
     2281 cacgagttcg agatcgaggg agagggagag ggacgtccat acgagggatt ccaaaccgcc
     2341 aagctcaagg tcaccaaggt aagtttaaac atatatatac taactaaccc tgattattta
     2401 aattttcagg gaggaccact cccattcgcc tgggacatcc tctccccaca cttcacctac
     2461 ggatccaagg cctacgtcaa gcacccagcc gacatcccag actacttcaa gctctccttc
     2521 ccagagggat tcaagtggga gcgtgtcatg aactacgagg acggaggagt cgtcaccgtc
     2581 acccaagact cctccctcca agacggagag ttcatctaca aggtaagttt aaacagttcg
     2641 gtactaacta accatacata tttaaatttt caggtcaagc tccgtggaac caacttccca
     2701 tccgacggac cagtcatgca aaagaagacc atgggatggg aggcctcctc cgagcgtatg
     2761 tacccagagg acggagccct caagggaaag atcaagatgc gtctcaagct caaggtaagt
     2821 ttaaacatga ttttactaac taactaatct gatttaaatt ttcaggacgg aggacactac
     2881 acctccgagg tcaagaccac ctacaaggcc aagaagccag tccaactccc aggagcctac
     2941 atcgtcgaca tcaagctcga catcacctcc cacaacgagg actacaccat cgtcgagcaa
     3001 tacgagcgtg ccgagggacg tcactccacc ggaggaatgg acgaactcta caagcttaac
     3061 ggtcctagga gatctgaatt cggcgcctaa gagctccgca tcggccgctg tcatcagatc
     3121 gccatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg
     3181 ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaaac ttcttcttaa
     3241 tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa
     3301 aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa
     3361 taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttttacttc
     3421 tgataaattt tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt
     3481 tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt
     3541 caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa
     3601 agtttatgaa attaattttc ctgcttttgc tttttggggg tttcccctat tgtttgtcaa
     3661 gagtttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag
     3721 aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt
     3781 tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat
     3841 accaaacata actgtttcct actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg
     3901 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     3961 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     4021 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     4081 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcggcct taagggcctc
     4141 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4201 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4261 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4321 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4381 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4441 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4501 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4561 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4621 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4681 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4741 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4801 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4861 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4921 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4981 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5041 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5101 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5161 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5221 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5281 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5341 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5401 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5461 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     5521 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5581 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5641 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5701 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     5761 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5821 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5881 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5941 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6001 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6061 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6121 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6181 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     6241 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     6301 tgtggaattg tgagcggata acaatttcac acaggaaaca gct
//
