LOCUS       Exported                6298 bp ds-DNA     circular SYN 25-JAN-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Pnhx2(XbaI mut)CemNeptune2_5.nucl Assembly
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 25, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    0..3051
                     /label=1378 to 1383 of mKate2 + SacI R
                     /note="1378 to 1383 of mKate2 + SacI R"
     misc_feature    0
                     /label=223 to 234 of E2-Crimson+NheI_F [Split]
                     /note="223 to 234 of E2-Crimson+NheI_F [Split]"
     misc_feature    complement(0)
                     /label=947 to 958 of E2-Crinson_+_SacI_R [Split]
                     /note="947 to 958 of E2-Crinson_+_SacI_R [Split]"
     misc_feature    24..2004
                     /label=pNHX-2
                     /note="1 to 1997 of 1.5kbPnhxSphXba [Split]"
     misc_feature    1953..3141
                     /label=NEPTUNE-2418
                     /note="NEPTUNE-2418"
     misc_feature    complement(1956..3080)
                     /label=NEPTUNE-3020
                     /note="NEPTUNE-3020"
     misc_feature    join(2089..6298,1..0)
                     /label=685 to 690 of mKate2 + NheI F
                     /note="685 to 690 of mKate2 + NheI F"
     misc_feature    2089..3051
                     /label=679 to 1389 of mKate2+NheI + SacI [Split]
                     /note="679 to 1389 of mKate2+NheI + SacI [Split]"
     misc_feature    2090..3050
                     /label=2 to 962 of CemNeptune2_5 gBlock
                     /note="2 to 962 of CemNeptune2_5 gBlock"
     CDS             2125..3045
                     /codon_start=1
                     /label=CemNeptune 2.5
                     /note="CemNeptune 2.5"
                     /translation="MVSKGEELIKENMHTKLYMEGTVNNHHFKCTHEGEGKPYEGTQTN
                     RIKVVEGGPLPFAFDILATCFMYGSKVSLNIYILTNPDYLNFQTFINHTQGIPDFFKQS
                     FPEGFTWERVTTYEDGGVLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKVSLNSSV
                     LTNHTYLNFQKTLGWEASTETLYPADGGLEGRCDMALKLVGGGHLHCNLKTTYRSKVSL
                     NMILLTN*SDLNFQKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAVARYCDLPS
                     KLGHKLNGMDELYKLNGPRRSEFGA"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgca gctttgtggc gaccaccacg tgtctactga
       61 taaattaaca gattgtacta ctttgcatgt aatgtgctag aggacggttc tcttatcatc
      121 acaggcactg acgacactgc caaagtttta caagctaatt tattagcgct cttcggccgc
      181 aatggcacga tttgaattgg tttattgatt ccagagacta gtttcagttt ttgataagaa
      241 tgaattatta ttcattttct agtttatgaa tatatatgta actagggaag cctatttatg
      301 atttttctaa ttttttaaaa ttgttatttt agagaggtgt gaaattaaaa tgatatatat
      361 tttcgagtac tttaaaaaat aaaaacggta catcctatat taaatttttg attttagatc
      421 cttccaagtt attgacaagt attaactgca cttctcataa aaatttttca aatagatcta
      481 tttagtctgg aataggaata cttttggcaa ttttcttgag aatcatagaa gacgaatttc
      541 tgaactgctc tttgtgaagc cacgcccata tattggcatt tttgtatcca attttttgta
      601 gagtacctga atcattatca ctacatctgc taaacaatat atggaagttc tatgagaggt
      661 gaaaggtaaa tcatataaca actgtaataa catagaaatg attgtagtat atttccaatt
      721 aggctgagtt gatcttattg aagagtttga atcatagcaa ttttttcctc ttttagtatt
      781 tttcaaaaca aattttcaca atgttagatg tagtatttag cattaaggca tctgtaatct
      841 cccagaaatg ttttgggaat aaaacccttt gactctttgg tccttttttg ctccaaataa
      901 tatctgattt taacccgttc tgatctactt tttacctttc gatcgtcaca tccgcgtaca
      961 cgtttctctt gctctcaatt gcttatcttt atggcctttt cccccacttt ttgtcgacta
     1021 tcatgcggac gtggcttcct tgctccctcc cggttttttt gaaaacactt gtgaatttat
     1081 tttttgtggt caaaattacg aaacatttga attctcaatc tatttataga ccgggtgtgg
     1141 tatgttatgc catgtttttg tcattgccaa atgaacatcg atattttaat tttctgaact
     1201 taccgtaccc cctgcgttac tattttattt ttaatttgta taatttcatt gctataattt
     1261 gttgtccttg aaacattgaa gatatcgaag aaataatgac aaaaatcaga tagaacaatt
     1321 tttcctactc atcaactcac atttttctat tttctcttgt gcgaaaaaaa tagtatattg
     1381 ggaatattta tagtattgca acttttctct tagttcgcag agttgcaata ctactagatc
     1441 cctaatagag gaaatatggt gaccagcttt ttgcaattgg aaagttggga aaaaagtata
     1501 tatattaata gacttacact cttgtagttc gtagaatttt tcaggaaatc tgacacgcaa
     1561 gactttttca aaaagttttt ttttttaatt tggcaatctg ccaaaacgtt tactgaacat
     1621 tttgaaaaca tctaccagta cctttaacac aacgaaaaat tgttctaagc aatattaact
     1681 gtgataattg atgttaagac ctgaaattta atcacaaatt tcacctggaa atgacagtaa
     1741 tcggcttcta aatactgtcg acacaagtgc catacctaag attccaatcc tattaccatg
     1801 tttcccaatt tcattctctt tcgtatctct atttctcttc tttattatca attacttttt
     1861 atcagttctt cgtttgcttt aacatcaaga gcacatagcg ctctctcgtc tctctctcat
     1921 gctctttttg aacttttaca aaaaactatt tccggttgtt ttgattcttg gaatttgaaa
     1981 taattttcag tgattaaatc tagaggatcc ccgggattgg ccaaaggacc caaaggtatg
     2041 tttcgaatga tactaacata acatagaaca ttttcaggag gacccttggc tagcctcgag
     2101 aggcctggta ccatgggcgg aaaaatggtc tccaagggag aggagcttat caaggagaac
     2161 atgcacacca agctctacat ggagggaacc gtcaacaacc accacttcaa gtgcacccac
     2221 gagggagagg gaaagccata cgagggaacc caaaccaacc gtatcaaggt cgtcgaggga
     2281 ggaccactcc cattcgcctt cgacatcctc gccacctgct tcatgtacgg atccaaggta
     2341 agtttaaaca tatatatact aactaaccct gattatttaa attttcagac cttcatcaac
     2401 cacacccaag gaatcccaga cttcttcaag caatccttcc cagagggatt cacctgggag
     2461 cgtgtcacca cctacgagga cggaggagtc ctcaccgtca cccaagacac ctccctccaa
     2521 gacggatgcc tcatctacaa cgtcaagctc cgtggagtca acttcccatc caacggacca
     2581 gtcatgcaaa aggtaagttt aaacagttcg gtactaacta accatacata tttaaatttt
     2641 cagaagaccc tcggatggga ggcctccacc gagaccctct acccagccga cggaggacta
     2701 gagggacgtt gcgacatggc cctcaagctc gtcggaggag gacacctcca ctgcaacctc
     2761 aagaccacct accgttccaa ggtaagttta aacatgattt tactaactaa ctaatctgat
     2821 ttaaattttc agaagccagc caagaacctc aagatgccag gagtctactt cgtcgaccgt
     2881 cgactagagc gtatcaagga ggccgacaac gagacctacg tcgagcaaca cgaggtcgcc
     2941 gtcgcccgtt actgcgacct cccatccaag ctcggacaca agctcaacgg aatggacgaa
     3001 ctctacaagc ttaacggtcc taggagatct gaattcggcg cctaagagct ccgcatcggc
     3061 cgctgtcatc agatcgccat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt
     3121 caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa
     3181 aaaacttctt cttaatttct ttgtttttta gcttctttta agtcacctct aacaatgaaa
     3241 ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc
     3301 tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt acacttctta
     3361 tgtttttttt acttctgata aatttttttt gaaacatcat agaaaaaacc gcacacaaaa
     3421 taccttatca tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg
     3481 ggcctctcat gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt
     3541 tttcaatcaa gtgaaagttt atgaaattaa ttttcctgct tttgcttttt gggggtttcc
     3601 cctattgttt gtcaagagtt tcgaggacgg cgtttttctt gctaaaatca caagtattga
     3661 tgagcacgat gcaagaaaga tcggaagaag gtttgggttt gaggctcagt ggaaggtgag
     3721 tagaagttga taatttgaaa gtggagtagt gtctatgggg tttttgcctt aaatgacaga
     3781 atacattccc aatataccaa acataactgt ttcctactag tcggccgtac gggccctttc
     3841 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     3901 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     3961 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     4021 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     4081 ggccttaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     4141 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4201 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4261 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4321 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4381 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4441 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4501 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4561 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4621 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4681 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4741 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4801 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4861 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4921 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4981 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5041 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5101 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5161 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5221 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5281 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5341 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5401 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     5461 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5521 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5581 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5641 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5701 gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5761 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5821 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5881 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5941 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6001 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6061 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6121 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6181 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     6241 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagct
//
