LOCUS       pKS_3HA_PAC             4189 bp ds-DNA     circular     15-JUN-2018
DEFINITION  pBluescript II KS(+) vector DNA, phagemid excised from lambda
            ZAPII.
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             752..850
                     /vntifkey="4"
                     /note="3HA epitope tag"
                     /label=3HA
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1257..1865
                     /vntifkey="21"
                     /label=PAC
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    983..1253
                     /label=gamma giardin 5'
                     /ApEinfo_fwdcolor=#ffff80
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    854..975
                     /label=alpha tub 3' UTR
                     /ApEinfo_fwdcolor=#408080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa
      721 gcttatcgat accgtcgacc tcgagcttaa ggaatatcct tatgacgtcc cagactacgc
      781 ggagtaccca tacgatgtac ctgattatgc tgagtaccca tacgacgtac cagattacgc
      841 tggatcctga atcgaattcT TTCCGTCGAC ctttgcatca tcgttgctcg gtcgtctgcc
      901 tcgtttacga cctgcttgtt cctgggcctc aaatggttag tggttttcct tcttccaacg
      961 gcgttggtcc atcctcggcc gcgtgatcaa atgccttcga gcgtatgtct gacctgtttg
     1021 tgaatgacga tccgtggctt tactcagatt cctgtgtttg tgaataagaa atccgtgaat
     1081 ttgttggggt ggaacggcga agtggcctct gctacaataa gaactggaga gtgtgttcag
     1141 aggcgaatgc gcaacgaact ttgtcgttac acgacgtgtg cgcctgtggt cttcgtgcgg
     1201 tttccgcgcg gaattttgcg ccttttctaa aaaactaacc ctaaaacaag aaaatcatgg
     1261 gcaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     1321 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     1381 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     1441 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     1501 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     1561 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     1621 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     1681 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     1741 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     1801 acgtcgagtg cccgaaggac cgcgcgacct ggtgcatgac ccgcaagccc ggtgccctcg
     1861 agtgagggct gaagtgaata tttacctttt ccgggttcga cgggcgtgct tgacgatgcg
     1921 gaagtcgctg tccgttccaa cggctatctg ccccaggacc cccatcccgg cgccgatcag
     1981 ctggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     2041 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     2101 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     2161 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     2221 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     2281 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2341 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2401 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2461 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2521 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2581 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2641 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2701 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2761 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2821 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2881 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2941 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3001 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3061 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3121 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3181 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3241 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3301 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3361 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3421 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3481 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3541 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3601 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3661 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3721 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3781 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3841 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3901 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3961 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4021 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4081 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4141 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac 
//
