LOCUS       pKS-3HA-BSR             4007 bp    DNA     circular      2-FEB-2009
DEFINITION  pBluescript II KS(+) vector DNA, phagemid excised from lambda ZAPII.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|500925296|
COMMENT     VNTDBDATE|508950191|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pKS-3HA-BSR|
COMMENT     VNTAUTHORNAME|Cande|
COMMENT     VNTOAUTHORNAME|NCBI Entrez|
COMMENT     VNTOAUTHORTEL|(301)496-2475|
COMMENT     VNTOAUTHORFAX|(301)480-9241|
COMMENT     VNTOAUTHOREML|info@ncbi.nlm.nih.gov|
COMMENT     VNTOAUTHORWWW|http://www3.ncbi.nlm.nih.gov/Entrez/|
COMMENT     VNTOAUTHORAD1|National Center for Biotechnology Information|
COMMENT     VNTOAUTHORAD2|National Library of Medicine|
COMMENT     VNTOAUTHORAD3|Building 38A, Room 8N805|
COMMENT     VNTOAUTHORAD4|Bethesda, MD 20894, U.S.A.|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pKS-3HA-BSR" 0 4007 
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COMMENT         "PstI" "SmaI" "XmaI" "BsrGI" "BsrI") (CStringList "atg" "gtg") 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 2 696 0 741 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 690 0 843 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 727 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 708 0 855 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 720 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 706 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 698 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 696 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "BsrGI" "tgtaca" 2 0 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "BsrI" "actgg" 7 5 13 141 1 587 1 613 1 1118 0 
COMMENT             2000 1 2608 0 2621 0 2733 1 3139 1 3257 1 3300 1 3569 0 3739 1 0 "")) 
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COMMENT             (CStringList) 1 1 1 1 "") 
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COMMENT                          "DNA Plasmid 'pKS-3HA-BSR'" 1) 
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COMMENT                          "pBluescript II KS(+) vector DNA, phagemid excised from lambda ZAPII" 
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COMMENT                          "Foreign object. Author: Cande. Original author: NCBI Entrez" 
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COMMENT                          "Created: 12/05/08 05:54PM" 1) 
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COMMENT                          "length: 4007 bp" 1) 
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FEATURES             Location/Qualifiers
     CDS             752..850
                     /vntifkey="4"
                     /label=3HA
                     /note="3HA epitope tag"
     CDS             1246..1683
                     /vntifkey="4"
                     /label=BSR
                     /note="blasticidin S resistance"
BASE COUNT     1025 a       955 c      1011 g      1016 t 
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca 
      661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa 
      721 gcttatcgat accgtcgacc tcgagcttaa ggaatatcct tatgacgtcc cagactacgc 
      781 ggagtaccca tacgatgtac ctgattatgc tgagtaccca tacgacgtac cagattacgc 
      841 tggatcctga atcgaattcc tttgcatcat cgttgctcgg tcgtctgcct cgtttacgac 
      901 ctgcttgttc ctgggcctca aatggttagt ggttttcctt cttccaacgg cgttggtcca 
      961 tcctcggccg cgtgatcaaa tgccttcgag cgtatgtctg acctgtttgt gaatgacgat 
     1021 ccgtggcttt actcagattc ctgtgtttgt gaataagaaa tccgtgaatt tgttggggtg 
     1081 gaacggcgaa gtggcctctg ctacaataag aactggagag tgtgttcaga ggcgaatgcg 
     1141 caacgaactt tgtcgttaca cgacgtgtgc gcctgtggtc ttcgtgcggt ttccgcgcgg 
     1201 aattttgcgc cttttctaaa aaactaaccc taaaacaaga aaatcatggg aatgaaaaca 
     1261 tttaacattt ctcaacaaga tctagaatta gtagaagtag cgacagagaa gattacaatg 
     1321 ctttatgagg ataataaaca tcatgtggga gcggcaattc gtacgaaaac aggagaaatc 
     1381 atttcggcag tacatattga agcgtatata ggacgagtaa ctgtttgtgc agaagccatt 
     1441 gcgattggta gtgcagtttc gaatggacaa aaggattttg acacgattgt agctgttaga 
     1501 cacccttatt ctgacgaagt agatagaagt attcgagtgg taagtccttg tggtatgtgt 
     1561 agggagttga tttcagacta tgcaccagat tgttttgtgt taatagaaat gaatggcaag 
     1621 ttagtcaaaa ctacgattga agaactcatt ccactcaaat atacccgaaa ttgagtcgag 
     1681 tgagggctga agtgaatatt taccttttcc gggttcgacg ggcgtgcttg acgatgcgga 
     1741 agtcgctgtc cgttccaacg gctatctgcc ccaggacccc catcccggcg ccgatcagct 
     1801 ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc 
     1861 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 
     1921 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 
     1981 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 
     2041 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 
     2101 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 
     2161 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 
     2221 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 
     2281 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 
     2341 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 
     2401 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 
     2461 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 
     2521 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 
     2581 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 
     2641 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 
     2701 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 
     2761 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 
     2821 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 
     2881 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 
     2941 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 
     3001 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 
     3061 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 
     3121 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 
     3181 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 
     3241 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 
     3301 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 
     3361 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 
     3421 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 
     3481 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 
     3541 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 
     3601 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 
     3661 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 
     3721 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 
     3781 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 
     3841 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 
     3901 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 
     3961 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac 
//
