LOCUS       pGT-Ah7                 5561 bp ds-DNA     circular SYN 25-JAN-2019
DEFINITION  .
ACCESSION   . (modified)
VERSION     .
KEYWORDS    pSAM_Bt.
SOURCE      null
  ORGANISM  
            .
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 06, 2014 from SnapGene Viewer 1.5.3
            http://www.snapgene.com
COMMENT     LOCUS PSAM_BT.GB 4582 BP SS-DNA CIRCULAR SYN 07-SEP-2006 DEFINITION
            - ACCESSION - KEYWORDS - SOURCE -.
FEATURES             Location/Qualifiers
     source          order(<2185..>2218,<1..1217)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     rep_origin      29..825
                     /created_by="User"
                     /modified_by="User"
                     /label="p15A"
     source          complement(<868..>1187)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     rep_origin      992..1090
                     /created_by="User"
                     /modified_by="User"
                     /label="RP4-oriT"
     origin          1217^1218
                     /label="origin"
     gene            complement(1375..2184)
                     /created_by="User"
                     /modified_by="User"
                     /label="kanR"
     promoter        complement(2219..2225)
                     /note="P3"
                     /label="P3 promoter"
     source          <2219..>5561
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     misc_feature    <2401..4395
                     /note="transposon"
                     /label="transposon"
     misc_feature    2401..2429
                     /note="MmeI IR_L"
                     /label="MmeI IR L"
     promoter        2557..2682
                     /created_by="User"
                     /modified_by="User"
                     /label="promoter 8 (MAGIC paper)"
     gene            2683..3306
                     /note="catP chloramphenicol acetyltransferase, cmlR
                     chloramphenicol resistance"
                     /modified_by="User"
                     /label="catP gene"
     misc_feature    3111..3116
                     /Recognition_pattern="GTY^RAC"
                     /annotation_group="GTYRAC"
                     /label="HincII"
                     /note="Geneious type: restriction site"
     RBS             3307..3342
                     /created_by="User"
                     /modified_by="User"
                     /label="RBS"
     source          <3307..>4401
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     source          <3307..>3342
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     gene            3343..4059
                     /created_by="User"
                     /label="sfGFP"
     source          order(<3343..>4059,<3343..>4059)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     misc_feature    3406..3411
                     /Recognition_pattern="GTY^RAC"
                     /annotation_group="GTYRAC"
                     /label="HincII"
                     /note="Geneious type: restriction site"
     terminator      4119..4162
                     /note="E. coli rrnB terminator T1"
                     /label="E. coli rrnB terminator T1"
     misc_feature    4367..4395
                     /note="MmeI IR_R"
                     /label="MmeI IR R"
     promoter        4402..4511
                     /created_by="User"
                     /label="promoter 6 (MAGIC)"
     gene            4515..5561
                     /note="mariner transposase himar1C9"
                     /label="mariner transposase himar1C9 gene"
     misc_feature    4940..4945
                     /Recognition_pattern="GTY^RAC"
                     /annotation_group="GTYRAC"
                     /label="HincII"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 gcggccgcca ccgcggtgga ggggaattaa caacttatat cgtatggggc tgacttcagg
       61 tgctacattt gaagagataa attgcactga aatctagaaa tattttatct gattaataag
      121 atgatcttct tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac
      181 cgccttgcag ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa
      241 ctggcttgga ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca
      301 tgacttcaag actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg
      361 catgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact
      421 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg
      481 tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
      541 gcaggaacag gagagcgcac gagggagccg ccagggggaa acgcctggta tctttatagt
      601 cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg
      661 cggagcctat ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc
      721 tggcatcttc caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa
      781 cgaccgagcg tagcgagtca gtgagcgagg aagcggaata tatccagatc ctctacgccg
      841 gacgcatcgt ggccggatca attccgtgat aggtgggctg cccttcctgg ttggcttggt
      901 ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag
      961 agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc
     1021 gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg
     1081 aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac
     1141 cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaacg gaattgatcc
     1201 ccctcgaatt gacgcgtaac cagcaataga cataagcggc tatttaacga ccctgccctg
     1261 aaccgacgac cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact
     1321 tattcaggcg tagcaaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattagaa
     1381 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     1441 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     1501 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     1561 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     1621 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
     1681 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     1741 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     1801 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     1861 taatacctgg aatgctgttt ttccggggat cgcagtggtg agtaaccatg catcatcagg
     1921 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     1981 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     2041 tggcgcatcg ggcttcccat acaagcgata gattgtcgca cctgattgcc cgacattatc
     2101 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga
     2161 cgtttcccgt tgaatatggc tcatactctt cctttttcaa tattattgaa gcatttatca
     2221 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2281 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     2341 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcacgcgt cctcggtacc
     2401 taacaggttg gatgataagt ccccggtctt cgtatgccgt cttctgcttg gcgcgccctc
     2461 gagcaattgc cgaccgaatt tttatgtcgt aaagaggggc tttgcagggg gtggactcag
     2521 aaagatgaga atagatgact attgtagttg aaacacgcaa gtgttcaaga agttattaag
     2581 tcgggagtgc agtcgaagtg ggcaagttga aaaattcaca aaaatgtggt ataatatctt
     2641 tgttcattag agcgataaac ttgaatttga gagggaactt agatggtatt tgaaaaaatt
     2701 gataaaaata gttggaacag aaaagagtat tttgaccact actttgcaag tgtaccttgt
     2761 acatacagca tgaccgttaa agtggatatc acacaaataa aggaaaaggg aatgaaacta
     2821 tatcctgcaa tgctttatta tattgcaatg attgtaaacc gccattcaga gtttaggacg
     2881 gcaatcaatc aagatggtga attggggata tatgatgaga tgataccaag ctatacaata
     2941 tttcacaatg atactgaaac attttccagc ctttggactg agtgtaagtc tgactttaaa
     3001 tcatttttag cagattatga aagtgatacg caacggtatg gaaacaatca tagaatggaa
     3061 ggaaagccaa atgctccgga aaacattttt aatgtatcta tgataccgtg gtcaaccttc
     3121 gatggcttta atctgaattt gcagaaagga tatgattatt tgattcctat ttttactatg
     3181 gggaaatatt ataaagaaga taacaaaatt atacttcctt tggcaattca agttcatcac
     3241 gcagtatgtg acggatttca catttgccgt tttgtaaacg aattgcagga attgataaat
     3301 agttaaatta tatttaaact caatttaaag tagggagaat agatgcgtaa aggcgaagag
     3361 ctgttcactg gtttcgtcac tattctggtg gaactggatg gtgatgtcaa cggtcataag
     3421 ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac gctgaagttc
     3481 atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac gctgacttat
     3541 ggtgttcagt gctttgctcg ttatccggac cacatgaagc agcatgactt cttcaagtcc
     3601 gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga cggcacgtac
     3661 aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat tgagctgaaa
     3721 ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata caattttaac
     3781 agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc gaattttaaa
     3841 attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca gcaaaacact
     3901 ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac gcaaagcgtt
     3961 ctgtctaaag atccgaacga gaaacgcgat cacatggttc tgctggagtt cgtaaccgca
     4021 gcgggcatca cgcatggtat ggatgaactg tacaaataaa aacaataggc cacatgcaac
     4081 tgtctagaat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga
     4141 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     4201 atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac
     4261 gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt
     4321 ttgcgtttct acctgcaggg cgcgccaagc agaagacggc atacgaagac cggggactta
     4381 tcatccaacc tgttaggatc cataaggatt ctttaaagag agatatagtt atgtcaaaga
     4441 ctgtagaatt tttagtaaat caaaataaaa aaagaggtat taaatagagt gtattttaaa
     4501 ggaggagact tcatatggaa aaaaaggaat ttcgtgtttt gataaaatac tgttttctga
     4561 agggaaaaaa tacagtggaa gcaaaaactt ggcttgataa tgagtttccg gactctgccc
     4621 cagggaaatc aacaataatt gattggtatg caaaattcaa gcgtggtgaa atgagcacgg
     4681 aggacggtga acgcagtgga cgcccgaaag aggtggttac cgacgaaaac atcaaaaaaa
     4741 tccacaaaat gattttgaat gaccgtaaaa tgaagttgat cgagatagca gaggccttaa
     4801 agatatcaaa ggaacgtgtt ggtcatatca ttcatcaata tttggatatg cggaagctct
     4861 gtgcgaaatg ggtgccgcgc gagctcacat ttgaccaaaa acaacgacgt gttgatgatt
     4921 ctaagcggtg tttgcagctg ttaactcgta atacacccga gtttttccgt cgatatgtga
     4981 caatggatga aacatggctc catcactaca ctcctgagtc caatcgacag tcggctgagt
     5041 ggacagcgac cggtgaaccg tctccgaagc gtggaaagac tcaaaagtcc gctggcaaag
     5101 taatggcctc tgttttttgg gatgcgcatg gaataatttt tatcgattat cttgagaagg
     5161 gaaaaaccat caacagtgac tattatatgg cgttattgga gcgtttgaag gtcgaaatcg
     5221 cggcaaaacg gccccacatg aagaagaaaa aagtgttgtt ccaccaagac aacgcaccgt
     5281 gccacaagtc attgagaacg atggcaaaaa ttcatgaatt gggcttcgaa ttgcttcccc
     5341 acccgccgta ttctccagat ctggccccca gcgacttttt cttgttctca gacctcaaaa
     5401 ggatgctcgc agggaaaaaa tttggctgca atgaagaggt gatcgccgaa actgaggcct
     5461 attttgaggc aaaaccgaag gagtactacc aaaatggtat caaaaaattg gaaggtcgtt
     5521 ataatcgttg tatcgctctt gaagggaact atgttgaata a
//
