LOCUS       pGT-B3                  6234 bp    DNA     circular UNA 20-DEC-2018
DEFINITION  pJN105 backbone, carb resistance, sfGFP.
ACCESSION   urn.local...1377632286368.18 (modified)
VERSION     urn.local...1377632286368.18
KEYWORDS    .
SOURCE      Expression vector pJN105
  ORGANISM  Expression vector pJN105
            other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     misc_feature    112..117
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /label="PvuI"
                     /note="Geneious type: restriction site"
     misc_feature    579..584
                     /Recognition_pattern="GCC^GGC"
                     /annotation_group="GCCGGC"
                     /label="NaeI"
                     /note="Geneious type: restriction site"
     gene            complement(721..1383)
                     /created_by="User"
                     /label="rep"
     misc_feature    1377..1382
                     /Recognition_pattern="TGG^CCA"
                     /annotation_group="TGGCCA"
                     /label="BalI"
                     /note="Geneious type: restriction site"
     misc_feature    2289..2294
                     /Recognition_pattern="GT^MKAC"
                     /annotation_group="GTMKAC"
                     /label="AccI"
                     /note="Geneious type: restriction site"
     rep_origin      2377..>3369
                     /created_by="User"
                     /modified_by="User"
                     /label="RP4 oriT"
     misc_feature    2851..2858
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     misc_feature    2853..2865
                     /Recognition_pattern="GGCCNNNN^NGGCC"
                     /annotation_group="GGCCNNNNNGGCC"
                     /label="SfiI"
                     /note="Geneious type: restriction site"
     origin          3175..3176
                     /label="misc"
     misc_feature    3456..3461
                     /Recognition_pattern="GAT^ATC"
                     /annotation_group="GATATC"
                     /label="EcoRV"
                     /note="Geneious type: restriction site"
     misc_feature    3650..3655
                     /Recognition_pattern="A^GATCT"
                     /annotation_group="AGATCT"
                     /label="BglII"
                     /note="Geneious type: restriction site"
     gene            complement(3656..4516)
                     /note="beta-lactamase"
                     /modified_by="User"
                     /label="beta-lactamase"
     misc_feature    4096..4101
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /label="PvuI"
                     /note="Geneious type: restriction site"
     misc_feature    4207..4212
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI"
                     /note="Geneious type: restriction site"
     misc_feature    4396..4401
                     /Recognition_pattern="G^TGCAC"
                     /annotation_group="GTGCAC"
                     /label="ApaLI"
                     /note="Geneious type: restriction site"
     promoter        complement(4517..4804)
                     /created_by="User"
                     /modified_by="User"
                     /label="promoter"
     misc_feature    4647..4652
                     /Recognition_pattern="GACGT^C"
                     /annotation_group="GACGTC"
                     /label="AatII"
                     /note="Geneious type: restriction site"
     misc_feature    4805..4810
                     /Recognition_pattern="C^CATGG"
                     /annotation_group="CCATGG"
                     /label="NcoI"
                     /note="Geneious type: restriction site"
     misc_feature    4928..4933
                     /Recognition_pattern="GCATG^C"
                     /annotation_group="GCATGC"
                     /label="SphI"
                     /note="Geneious type: restriction site"
     misc_feature    5203..5208
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    5218..5223
                     /Recognition_pattern="C^TCGAG"
                     /annotation_group="CTCGAG"
                     /label="XhoI"
                     /note="Geneious type: restriction site"
     misc_feature    5224..5229
                     /Recognition_pattern="GT^MKAC"
                     /annotation_group="GTMKAC"
                     /label="AccI"
                     /note="Geneious type: restriction site"
     misc_feature    5224..5229
                     /Recognition_pattern="G^TCGAC"
                     /annotation_group="GTCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     misc_feature    5224..5229
                     /Recognition_pattern="GTY^RAC"
                     /annotation_group="GTYRAC"
                     /label="HincII"
                     /note="Geneious type: restriction site"
     misc_feature    5233..5238
                     /Recognition_pattern="AT^CGAT"
                     /annotation_group="ATCGAT"
                     /label="ClaI"
                     /note="Geneious type: restriction site"
     misc_feature    5314..5319
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     promoter        5320..5484
                     /created_by="User"
                     /modified_by="User"
                     /label="promoter 3"
     gene            <5485..6219
                     /modified_by="User"
                     /label="sfGFP"
     misc_feature    5500..5505
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
     misc_feature    5566..5571
                     /Recognition_pattern="GTY^RAC"
                     /annotation_group="GTYRAC"
                     /label="HincII"
                     /note="Geneious type: restriction site"
     misc_feature    5664..5669
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    6223..6228
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
       61 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      121 ttcccaacag ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta
      181 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgactgcgat
      241 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
      301 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga
      361 cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac
      421 cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt
      481 tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca
      541 gagcctgata aaaacggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag
      601 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
      661 aggcggctac agccgatagt ctggaacagc gcacttacgg gttgctgcgc aacccaagtg
      721 ctaccggcgc ggcagcgtga cccgtgtcgg cggctccaac ggctcgccat cgtccagaaa
      781 acacggctca tcgggcatcg gcaggcgctg ctgcccgcgc cgttcccatt cctccgtttc
      841 ggtcaaggct ggcaggtctg gttccatgcc cggaatgccg ggctggctgg gcggctcctc
      901 gccggggccg gtcggtagtt gctgctcgcc cggatacagg gtcgggatgc ggcgcaggtc
      961 gccatgcccc aacagcgatt cgtcctggtc gtcgtgatca accaccacgg cggcactgaa
     1021 caccgacagg cgcaactggt cgcggggctg gccccacgcc acgcggtcat tgaccacgta
     1081 ggccgacacg gtgccggggc cgttgagctt cacgacggag atccagcgct cggccaccaa
     1141 gtccttgact gcgtattgga ccgtccgcaa agaacgtccg atgagcttgg aaagtgtctt
     1201 ctggctgacc accacggcgt tctggtggcc catctgcgcc acgaggtgat gcagcagcat
     1261 tgccgccgtg ggtttcctcg caataagccc ggcccacgcc tcatgcgctt tgcgttccgt
     1321 ttgcacccag tgaccgggct tgttcttggc ttgaatgccg atttctctgg actgcgtggc
     1381 catgcttatc tccatgcggt agggtgccgc acggttgcgg caccatgcgc aatcagctgc
     1441 aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc aattacagat
     1501 tttctttaac ctacgcaatg agctattgcg gggggtgccg caatgagctg ttgcgtaccc
     1561 ccctttttta agttgttgat ttttaagtct ttcgcatttc gccctatatc tagttctttg
     1621 gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg ctccccctcc
     1681 ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg cggccttcgg ccttgcccaa
     1741 ggtggcgctg cccccttgga acccccgcac tcgccgccgt gaggctcggg gggcaggcgg
     1801 gcgggcttcg ccttcgactg cccccactcg cataggcttg ggtcgttcca ggcgcgtcaa
     1861 ggccaagccg ctgcgcggtc gctgcgcgag ccttgacccg ccttccactt ggtgtccaac
     1921 cggcaagcga agcgcgcagg ccgcaggccg gaggcttttc cccagagaaa attaaaaaaa
     1981 ttgatggggc aaggccgcag gccgcgcagt tggagccggt gggtatgtgg tcgaaggctg
     2041 ggtagccggt gggcaatccc tgtggtcaag ctcgtgggca ggcgcagcct gtccatcagc
     2101 ttgtccagca gggttgtcca cgggccgagc gaagcgagcc agccggtggc cgctcgcggc
     2161 catcgtccac atatccacgg gctggcaagg gagcgcagcg accgcgcagg gcgaagcccg
     2221 gagagcaagc ccgtagggcg ccgcagccgc cgtaggcggt cacgactttg cgaagcaaag
     2281 tctagtgagt atactcaagc attgagtggc ccgccggagg caccgccttg cgctgccccc
     2341 gtcgagccgg ttggacacca aaagggaggg gcaggcatgg cggcatacgc gatcatgcga
     2401 tgcaagaagc tggcgaaaat gggcaacgtg gcggccagtc tcaagcacgc ctaccgcgag
     2461 cgcgagacgc ccaacgctga cgccagcagg acgccagaga acgagcactg ggcggccagc
     2521 agcaccgatg aagcgatggg ccgactgcgc gagttgctgc cagagaagcg gcgcaaggac
     2581 gctgtgttgg cggtcgagta cgtcatgacg gccagcccgg aatggtggaa gtcggccagc
     2641 caagaacagc aggcggcgtt cttcgagaag gcgcacaagt ggctggcgga caagtacggg
     2701 gcggatcgca tcgtgacggc cagcatccac cgtgacgaaa ccagcccgca catgaccgcg
     2761 ttcgtggtgc cgctgacgca ggacggcagg ctgtcggcca aggagttcat cggcaacaaa
     2821 gcgcagatga cccgcgacca gaccacgttt gcggccgctg tggccgatct agggctgcaa
     2881 cggggcatcg agggcagcaa ggcacgtcac acgcgcattc aggcgttcta cgaggccctg
     2941 gagcggccac cagtgggcca cgtcaccatc agcccgcaag cggtcgagcc acgcgcctat
     3001 gcaccgcagg gattggccga aaagctggga atctcaaagc gcgttgagac gccggaagcc
     3061 gtggccgacc ggctgacaaa agcggttcgg caggggtatg agcctgccct acaggccgcc
     3121 gcaggagcgc gtgagatgcg caagaaggcc gatcaagccc aagagacggc ccgagacctt
     3181 cgggagcgcc tgaagcccgt tctggacgcc ctggggccgt tgaatcggga tatgcaggcc
     3241 aaggccgccg cgatcatcaa ggccgtgggc gaaaagctgc tgacggaaca gcgggaagtc
     3301 cagcgccaga aacaggccca gcgccagcag gaacgcgggc gcgcacattt ccccgaaaag
     3361 tgccacctgc ggcgttgtga caatttaccg aacaactccg cggccgggaa gccgatctcg
     3421 gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc
     3481 cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg
     3541 tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg
     3601 gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata gatctttacc
     3661 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3721 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3781 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3841 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3901 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3961 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4021 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4081 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4141 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4201 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4261 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4321 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4381 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4441 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4501 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4561 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4621 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     4681 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     4741 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     4801 ggagccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag
     4861 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag
     4921 tttttatgca tgcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     4981 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     5041 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     5101 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     5161 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tgggtaccgg gccccccctc
     5221 gaggtcgacg gtatcgatgc ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa
     5281 accctatgct actccgtcaa gccgtcactc attggatccg tgaaggatac ggctgcggca
     5341 cttcgacatc gccccatgtg gcggctttga actgggctta tgaaacgcgt tcacaacctt
     5401 ttttgaccat cggcgcgaac gtggtatcat gcgttcagct tttgcccata catactacgt
     5461 gctcaatcta ggaggatttc atacatgcgt gatcattttc tgcagcgtaa aggcgaagag
     5521 ctgttcactg gtttcgtcac tattctggtg gaactggatg gtgatgtcaa cggtcataag
     5581 ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac gctgaagttc
     5641 atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac gctgacttat
     5701 ggtgttcagt gctttgctcg ttatccggac cacatgaagc agcatgactt cttcaagtcc
     5761 gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga cggcacgtac
     5821 aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat tgagctgaaa
     5881 ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata caattttaac
     5941 agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc gaattttaaa
     6001 attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca gcaaaacact
     6061 ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac gcaaagcgtt
     6121 ctgtctaaag atccgaacga gaaacgcgat cacatggttc tgctggagtt cgtaaccgca
     6181 gcgggcatca cgcatggtat ggatgaactg tacaaataac gcgaattcca gcac
//
