LOCUS       pTn7·xylS               4976 bp ds-DNA     circular     16-JAN-2019
DEFINITION  .
ACCESSION   KR920750                                                           
VERSION     KR920750.2                                                         
KEYWORDS    "Responsible person/group" "Usage" "Status" "Parent plasmids"      
            "Cloning sites" "Antibiotic resistance" "Origin of replication"
            "Promoters" "RBS" "Genes" "Tags" "Markers" "Spacers" "gRNAs"
            "Terminators" "DNA fragments" "Backbones" "Design entry"
            "LabCollector ID" "Comment"
SOURCE      Cloning vector pTn7-M                                              
  ORGANISM  Cloning vector pTn7-M other sequences; artificial sequences;       
            vectors.
FEATURES             Location/Qualifiers
     oriT            4647..4892
                     /label="oriT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     gene            3811..4626
                     /label="aphA gene"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             3811..4626
                     /label="aphA CDS"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(1439..2404)
                     /label="xylS CDS"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3400..3502
                     /label="T0"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    499..603
                     /label="T1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     gene            complement(1439..2404)
                     /label="xylS gene"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      join(4909..4976,1..324)
                     /label="R6K"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(664..1197)
                     /label="aacC1 CDS"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1421..3286
                     /label="Fragment2"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     gene            complement(664..1197)
                     /label="aacC1 gene"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3503..3701
                     /label="Tn7R"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    333..498
                     /label="Tn7L"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    join(3287..4976,1..1420)
                     /label="Fragment1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 ctcatgttta acgtactaag ctctcatgtt taacgaacta aaccctcatg gctaacgtac
       61 taagctctca tggctaacgt actaagctct catgtttcac gtactaagct ctcatgtttg
      121 aacaataaaa ttaatataaa tcagcaactt aaatagcctc taaggtttta agttttataa
      181 gaaaaaaaag aatatataag gcttttaaag cctttaaggt ttaacggttg tggacaacaa
      241 gccagggatg taacgcactg agaagccctt agagcctctc aaagcaattt tgagtgacac
      301 aggaacactt aacggctgac atggggcgcg cctgtgggcg gacaaaatag ttgggaactg
      361 ggaggggtgg aaatggagtt tttaaggatt atttagggaa gagtgacaaa atagatggga
      421 actgggtgta gcgtcgtaag ctaatacgaa aattaaaaat gacaaaatag tttggaacta
      481 gatttcactt atctggtttc tagggcggcg gatttgtcct actcaggaga gcgttcaccg
      541 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
      601 gccgacgtcc aatttaccga acaactccgc ggccgggaag ccgatctcgg cttgaacgaa
      661 ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc gtatgcccaa
      721 ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt agatcacata
      781 agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg caatgccctg
      841 cctccggtgc tctccggaga ctgcgagatc atagatatag atctcactac gcggctgctc
      901 aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt cgaaggcagc
      961 aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt ccggctgatg
     1021 ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag cagcccgcat
     1081 ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac ttgagccacc
     1141 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc gtaacatcgt
     1201 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
     1261 gaggcatagg ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt
     1321 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc
     1381 attacagttt acgaaccgaa caggcttatg tcaagacgtc ttaattaaac gttcgtaatc
     1441 aagccacttc ctttttgcat tgacgcaggg tgtcggaagg caactcgccg aacgcgctcc
     1501 tatagttttc agcgaagcgt cccaaatgta agaagccgta gtctagggct atctcagtta
     1561 tactacgcac attggcactg ggatcgttca agcaggcgcg gatgctttcg agcttgcggt
     1621 tgcggatgta gttcttcggc gtggtgccgg cgtgcttctc gaacaaattg tagagcgagc
     1681 gtggactcat catcgccagc tccgctaacc gctcaaggct gatattccgt ttgagattct
     1741 cctcaatgaa ttgaacgact cgctcgaaag acgggttacc tttgctgaaa atttcacggc
     1801 tgacattgct gcccagcatt tcgagcagct tggaagcgat gatccccgca tagtgctctt
     1861 ggacccgagg catcgacttt gtatgttccg cttcgtcaca aactaacccg agtagattga
     1921 taaagccatc gagttgctgg agattgtgtc gcgcggcgaa acggataccc tccctcggct
     1981 tgtgccaatt gttgtcactg cacgcccgat caaggaccac tgagggcaat ttaacgataa
     2041 atttctcgca atcttctgaa taggtcaggt cggcttggtc atccggattg agcagcaata
     2101 gttcgcccgg cgcaaaatag tgctcctggc catggccacg ccacaggcaa tggcctttga
     2161 gtattatttg cagatgataa caggtctcta atccaggcga gattaccctc acgctaccgc
     2221 cgtagctgat tcgacacaga tcgaggcatc cgaagattct gtggtgcagc ctgcctgccg
     2281 ggcgcccgcc cttgggcagg cgaatagagt gcgtaccgac atactggtta acataatcgg
     2341 agactgcata gggctcggcg tggacgaaga tctgactttt ctcgttcaat aagcaaaaat
     2401 ccatagttca cggttctctt attttaatgt gggctgcttg gtgtgatgta gaaaggcgcc
     2461 aagtcgatga aaatgcatct cgacgtgatg cgtatacggg ttacccccat tgccacgttg
     2521 cgccatcctt tttgcaatca gtgaccactt ttccaagcaa aaataacgcc aagcagaacg
     2581 aagacgttct ttttaagaag cgagaacacc agaagttcgt gctgtcgggg catggggcga
     2641 cgaattggcg gataaagggg atctgctgga tattacggcc tttttaaaga ccgtaaagaa
     2701 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     2761 tccgtaatta cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     2821 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     2881 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     2941 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
     3001 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     3061 tttcaccatg catgggaatt agcttgatct gaccaacgac cggtagcgga gctatccaac
     3121 ggcggtatac caggaaaaca cacagcaggt acatcagaac agtaccatga ctgaagaaca
     3181 aatagttttt tcctgatcca taaagcagaa cggcctgctc catgacaaat ctggctcccc
     3241 aactaatgcc ccatgcagcc agcataacca gcataaagtg cagtgtccta ggccgcggcc
     3301 gcgcgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct
     3361 tgcggccgcg tcgtgactgg gaaaaccctg gcgactagtc ttggactcct gttgatagat
     3421 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
     3481 ttttttattg gtgagaatcc agccacgccc ctctttaata cgacgggcaa tttgcacttc
     3541 agaaaatgaa gagtttgctt tagccataac aaaagtccag tatgcttttt cacagcataa
     3601 ctggactgat ttcagtttac aactattctg tctagtttaa gactttattg tcatagttta
     3661 gatctatttt gttcagttta agactttatt gtccgcccac aatttaaatt tgtgtctcaa
     3721 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     3781 gcttacataa acagtaatac aaggggtgtt atgagccata ttcagcgtga aacgagctgt
     3841 agccgtccgc gtctgaacag caacatggat gcggatctgt atggctataa atgggcgcgt
     3901 gataacgtgg gtcagagcgg cgcgaccatt tatcgtctgt atggcaaacc ggatgcgccg
     3961 gaactgtttc tgaaacatgg caaaggcagc gtggcgaacg atgtgaccga tgaaatggtg
     4021 cgtctgaact ggctgaccga atttatgccg ctgccgacca ttaaacattt tattcgcacc
     4081 ccggatgatg cgtggctgct gaccaccgcg attccgggca aaaccgcgtt tcaggtgctg
     4141 gaagaatatc cggatagcgg cgaaaacatt gtggatgcgc tggccgtgtt tctgcgtcgt
     4201 ctgcatagca ttccggtgtg caactgcccg tttaacagcg atcgtgtgtt tcgtctggcc
     4261 caggcgcaga gccgtatgaa caacggcctg gtggatgcga gcgattttga tgatgaacgt
     4321 aacggctggc cggtggaaca ggtgtggaaa gaaatgcata aactgctgcc gtttagcccg
     4381 gatagcgtgg tgacccacgg cgattttagc ctggataacc tgattttcga tgaaggcaaa
     4441 ctgattggct gcattgatgt gggccgtgtg ggcattgcgg atcgttatca ggatctggcc
     4501 attctgtgga actgcctggg cgaatttagc ccgagcctgc aaaaacgtct gtttcagaaa
     4561 tatggcattg ataatccgga tatgaacaaa ctgcaatttc atctgatgct ggatgaattt
     4621 ttctaataat taattggacc gcggtccttt tccgctgcat aaccctgctt cggggtcatt
     4681 atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt ttgccaaagg
     4741 gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta
     4801 ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgcacct ggcggtgctc
     4861 aacgggaatc ctgctctgcg aggctggccg taggccggcc ggcgcgccga tctgaagatc
     4921 agcagttcaa cctgttgata gtacgtacta agctctcatg tttcacgtac taagct
//