LOCUS       pBT29-splitD_(2nd_gener 5662 bp ds-DNA     circular     22-JAN-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     SNP              466..466
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     SNP              1827..1827
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     SNP              112..112
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     CDS              1532..1633
                      /label="gVII"
                      /ApEinfo_revcolor=#b1ff67
                      /ApEinfo_fwdcolor=#b1ff67
     SNP              1594..1594
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     RBS              1619..1626
                      /label="gIX RBS"
                      /ApEinfo_revcolor=#75c6a9
                      /ApEinfo_fwdcolor=#75c6a9
     SNP              354..354
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     promoter         1210..1259
                      /label="gV promoter"
                      /ApEinfo_revcolor=#ff9ccd
                      /ApEinfo_fwdcolor=#ff9ccd
     SNP              1557..1557
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     SNP              1440..1440
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     RBS              1252..1262
                      /label="gV RBS"
                      /ApEinfo_revcolor=#f58a5e
                      /ApEinfo_fwdcolor=#f58a5e
     cloning junction 1992..1994
                      /label="AGT for SapI Golden Gate cloning"
                      /ApEinfo_revcolor=#c6c9d1
                      /ApEinfo_fwdcolor=#c6c9d1
     SNP              1615..1615
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     CDS              23..1255
                      /label="gII"
                      /ApEinfo_revcolor=#b7e6d7
                      /ApEinfo_fwdcolor=#b7e6d7
     SNP              1680..1680
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     SNP              1360..1360
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     SNP              694..694
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     SNP              455..455
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     promoter         218..266
                      /label="gII' promoter (internal)"
                      /ApEinfo_revcolor=#f8d3a9
                      /ApEinfo_fwdcolor=#f8d3a9
     promoter         1579..1625
                      /label="gVIII promoter"
                      /ApEinfo_revcolor=#d6b295
                      /ApEinfo_fwdcolor=#d6b295
     SNP              757..757
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     cloning junction 1..3
                      /label="CTT for SapI Golden Gate cloning"
                      /ApEinfo_revcolor=#75c6a9
                      /ApEinfo_fwdcolor=#75c6a9
     RBS              10..19
                      /label="gII RBS"
                      /ApEinfo_revcolor=#ffef86
                      /ApEinfo_fwdcolor=#ffef86
     SNP              1943..1943
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     SNP              205..205
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     CDS              1725..1946
                      /label="gVIII"
                      /ApEinfo_revcolor=#f8d3a9
                      /ApEinfo_fwdcolor=#f8d3a9
     CDS              920..1255
                      /label="gX"
                      /ApEinfo_revcolor=#b7e6d7
                      /ApEinfo_fwdcolor=#b7e6d7
     CDS              1630..1728
                      /label="gIX"
                      /ApEinfo_revcolor=#84b0dc
                      /ApEinfo_fwdcolor=#84b0dc
     promoter         1924..1971
                      /label="gIII promoter"
                      /ApEinfo_revcolor=#f8d3a9
                      /ApEinfo_fwdcolor=#f8d3a9
     RBS              1710..1722
                      /label="gVIII RBS"
                      /ApEinfo_revcolor=#c7b0e3
                      /ApEinfo_fwdcolor=#c7b0e3
     CDS              1267..1530
                      /label="gV"
                      /ApEinfo_revcolor=#f58a5e
                      /ApEinfo_fwdcolor=#f58a5e
     RBS              905..916
                      /label="gX RBS"
                      /ApEinfo_revcolor=#84b0dc
                      /ApEinfo_fwdcolor=#84b0dc
     terminator       1962..1989
                      /label="terminator 1"
                      /ApEinfo_revcolor=#c6c9d1
                      /ApEinfo_fwdcolor=#c6c9d1
     SNP              46..46
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     RBS              1521..1527
                      /label="gVII RBS"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     SNP              914..914
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     SNP              773..773
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     promoter         805..852
                      /label="gX promoter"
                      /ApEinfo_revcolor=#c6c9d1
                      /ApEinfo_fwdcolor=#c6c9d1
     SNP              325..325
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     SNP              951..951
                      /label="mutated vs generation 1 backbone"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     cloning junction 3857..3872
                      /label="USER junction"
                      /ApEinfo_revcolor=#b7e6d7
                      /ApEinfo_fwdcolor=#b7e6d7
     restriction enzyme recognition sequence 5655..5661
                      /label="SapI"
                      /ApEinfo_revcolor=#84b0dc
                      /ApEinfo_fwdcolor=#84b0dc
     terminator       2760..2897
                      /label="terminator"
                      /ApEinfo_revcolor=#d59687
                      /ApEinfo_fwdcolor=#d59687
     promoter         2003..2081
                      /label="tet promoter"
                      /ApEinfo_revcolor=#d59687
                      /ApEinfo_fwdcolor=#d59687
     restriction enzyme recognition sequence complement(1996..2002)
                      /label="SapI"
                      /ApEinfo_revcolor=#84b0dc
                      /ApEinfo_fwdcolor=#84b0dc
     cloning junction 5573..5587
                      /label="USER junction"
                      /ApEinfo_revcolor=#faac61
                      /ApEinfo_fwdcolor=#faac61
     cloning junction 2895..2911
                      /label="USER junction"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     CDS              2082..2759
                      /label="mRFP"
                      /ApEinfo_revcolor=#ff9ccd
                      /ApEinfo_fwdcolor=#ff9ccd
     misc_feature     complement(2778..2790)
                      /label="T-rich"
                      /ApEinfo_revcolor=#f8d3a9
                      /ApEinfo_fwdcolor=#f8d3a9
     terminator       2777..2821
                      /label="rrnB1 rho-independent transcriptional terminator"
                      /ApEinfo_revcolor=#d59687
                      /ApEinfo_fwdcolor=#d59687
     misc_feature     2786..2817
                      /label="stem loop"
                      /ApEinfo_revcolor=#ff9ccd
                      /ApEinfo_fwdcolor=#ff9ccd
     misc_feature     complement(3804..3812)
                      /label="-10"
                      /ApEinfo_revcolor=#c7b0e3
                      /ApEinfo_fwdcolor=#c7b0e3
     misc_feature     complement(3806..3856)
                      /label="promoter (functional)"
                      /ApEinfo_revcolor=#75c6a9
                      /ApEinfo_fwdcolor=#75c6a9
     misc_feature     complement(3828..3833)
                      /label="-35"
                      /ApEinfo_revcolor=#c7b0e3
                      /ApEinfo_fwdcolor=#c7b0e3
     CDS              complement(2990..3805)
                      /label="aph (KanR)"
                      /ApEinfo_revcolor=#ffef86
                      /ApEinfo_fwdcolor=#ffef86
     rep_origin       complement(4827..5572)
                      /label="pSC101 ori"
                      /ApEinfo_revcolor=#c7b0e3
                      /ApEinfo_fwdcolor=#c7b0e3
     misc_feature     5274..5454
                      /label="partitioning locus, gyrase binding"
                      /ApEinfo_revcolor=#9eafd2
                      /ApEinfo_fwdcolor=#9eafd2
     CDS              complement(3873..4823)
                      /label="repA"
                      /ApEinfo_revcolor=#d59687
                      /ApEinfo_fwdcolor=#d59687
     misc_feature     5372..5394
                      /label="hairpin"
                      /ApEinfo_revcolor=#ff9ccd
                      /ApEinfo_fwdcolor=#ff9ccd
     misc_feature     complement(5589..5601)
                      /label="T-rich"
                      /ApEinfo_revcolor=#f8d3a9
                      /ApEinfo_fwdcolor=#f8d3a9
     terminator       5588..5632
                      /label="rrnB1 rho-independent transcriptional terminator"
                      /ApEinfo_revcolor=#d59687
                      /ApEinfo_fwdcolor=#d59687
     misc_feature     5597..5628
                      /label="stem loop"
                      /ApEinfo_revcolor=#ff9ccd
                      /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 cttattatca accggggtac atatgattga catgctagtt ttacgGttac cgttcatcga
       61 ttctcttgtt tgctccagac tctcaggcaa tgacctgata gcctttgtag aTctctcaaa
      121 aatagctacc ctctccggca tgaatttatc agctagaacg gttgaatatc atgttgatgg
      181 tgatttgact gtctccggcc tttcCcaccc ttttgaatct ttacctacac attactcagg
      241 cattgcattt aaaatatatg agggttctaa aaatttttat ccttgcgttg aaataaaggc
      301 ttctcccgca aaagtattac agggGcataa tgtttttggt acaaccgatt tagGtttatg
      361 ctctgaggct ttattgctta attttgctaa ttctttgcct tgcctgtatg atttattgga
      421 tgttaacgct actactatta gtagaattga tgccGccttt tcagcCcgcg ccccaaatga
      481 aaatatagct aaacaggtta ttgaccattt gcgaaatgta tctaatggtc aaactaaatc
      541 tactcgttcg cagaattggg aatcaactgt tacatggaat gaaacttcca gacaccgtac
      601 tttagttgca tatttaaaac atgttgagct acagcaccag attcagcaat taagctctaa
      661 gccatccgca aaaatgacct cttatcaaaa ggaAcaatta aaggtactct ctaatcctga
      721 cctgttggag tttgcttccg gtctggttcg ctttgaGgct cgaattaaaa cgAgatattt
      781 gaagtctttc gggcttcctc ttaatctttt tgatgcaatc cgctttgctt ctgactataa
      841 tagtcagggt aaagacctga tttttgattt atggtcattc tcgttttctg aactgtttaa
      901 agcatttgag gggAattcaa tgaatattta tgacgattcc gcagtattgg Ccgctatcca
      961 gtctaaacat tttactatta ccccctctgg caaaacttct tttgcaaaag cctctcgcta
     1021 ttttggtttt tatcgtcgtc tggtaaacga gggttatgat agtgttgctc ttactatgcc
     1081 tcgtaattcc ttttggcgtt atgtatctgc attagttgaa tgtggtattc ctaaatctca
     1141 actgatgaat ctttctacct gtaataatgt tgttccgtta gttcgtttta ttaacgtaga
     1201 tttttcttcc caacgtcctg actggtataa tgagccagtt cttaaaatcg cataaggtaa
     1261 ttcacaatga ttaaagttga aattaaacca tctcaagccc aatttactac tcgttctggt
     1321 gtttctcgtc agggcaagcc ttattcactg aatgagcagT tttgttacgt tgatttgggt
     1381 aatgaatatc cggttcttgt caagattact cttgatgaag gtcagccagc ctatgcgccG
     1441 ggtctgtaca ccgttcatct gtcctctttc aaagttggtc agttcggttc ccttatgatt
     1501 gaccgtctgc gcctcgttcc ggctaagtaa catggagcag gtcgcggatt tcgacaTaat
     1561 ttatcaggcg atgatacaaa tctccgttgt actCtgtttc gcgcttggta taatAgctgg
     1621 gggtcaaaga tgagtgtttt agtgtattct ttcgcctctt tcgttttagg ttggtgcctC
     1681 cgtagtggca ttacgtattt tacccgttta atggaaactt cctcatgaaa aagtctttag
     1741 tcctcaaagc ctctgtagcc gttgctaccc tcgttccgat gctgtctttc gctgctgagg
     1801 gtgacgatcc cgcaaaagcg gcctttGact ccctgcaagc ctcagcgacc gaatatatcg
     1861 gttatgcgtg ggcgatggtt gttgtcattg tcggcgcaac tatcggtatc aagctgttta
     1921 agaaattcac ctcgaaagca agTtgataaa cTgatacaat taaaggctcc ttttggagcc
     1981 ttttttttTg gagtAgaaga gcTccctatc agtgatagag attgacatcc ctatcagtga
     2041 tagagatact gagcacgaat ctgaaagagg agaaaggatc tatggcgagt agcgaagacg
     2101 ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt
     2161 tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga
     2221 aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt
     2281 acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct
     2341 tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg
     2401 ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta
     2461 ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca
     2521 ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
     2581 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
     2641 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
     2701 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
     2761 aaggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     2821 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     2881 gcctttctgc gtttAtacag gtggcacttt Tcggggaaat gtgcgcggaa cccctatttg
     2941 tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact
     3001 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     3061 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     3121 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     3181 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     3241 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     3301 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     3361 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     3421 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     3481 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     3541 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     3601 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     3661 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     3721 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag
     3781 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     3841 gttttattgt tcatgaAggc caaataggcc gTtcagatcc ttccgtattt agccagtatg
     3901 ttctctagtg tggttcgttg tttttgcgtg agccatgaga acgaaccatt gagatcatgc
     3961 ttactttgca tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt
     4021 aaagcatcgt gtagtgtttt tcttagtccg ttacgtaggt aggaatctga tgtaatggtt
     4081 gttggtattt tgtcaccatt catttttatc tggttgttct caagttcggt tacgagatcc
     4141 atttgtctat ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca
     4201 accaccaatt tcatattgct gtaagtgttt aaatctttac ttattggttt caaaacccat
     4261 tggttaagcc ttttaaactc atggtagtta ttttcaagca ttaacatgaa cttaaattca
     4321 tcaaggctaa tctctatatt tgccttgtga gttttctttt gtgttagttc ttttaataac
     4381 cactcataaa tcctcataga gtatttgttt tcaaaagact taacatgttc cagattatat
     4441 tttatgaatt tttttaactg gaaaagataa ggcaatatct cttcactaaa aactaattct
     4501 aatttttcgc ttgagaactt ggcatagttt gtccactgga aaatctcaaa gcctttaacc
     4561 aaaggattcc tgatttccac agttctcgtc atcagctctc tggttgcttt agctaataca
     4621 ccataagcat tttccctact gatgttcatc atctgagcgt attggttata agtgaacgat
     4681 accgtccgtt ctttccttgt agggttttca atcgtggggt tgagtagtgc cacacagcat
     4741 aaaattagct tggtttcatg ctccgttaag tcatagcgac taatcgctag ttcatttgct
     4801 ttgaaaacaa ctaattcaga catacatctc aattggtcta ggtgatttta atcactatac
     4861 caattgagat gggctagtca atgataatta ctagtccttt tcctttgagt tgtgggtatc
     4921 tgtaaattct gctagacctt tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat
     4981 tccgctagac ctttgtgtgt tttttttgtt tatattcaag tggttataat ttatagaata
     5041 aagaaagaat aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa ctcactactt
     5101 tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac
     5161 agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa atcgctgaat
     5221 attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg acattcagtt
     5281 cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc gccttttatg
     5341 gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct tctcagggcg
     5401 ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc agttcctgcc
     5461 ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc agactggcta
     5521 atgcacccag taaggcagcg gtatcatcaa caggcttacc cgtcttactg tcaagaggac
     5581 atccggtcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttgctgagga
     5641 gacttaggga ccctgctctt ct
//