LOCUS       pBT44c_intC.dCas9.UGI   6357 bp ds-DNA     circular     22-JAN-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS              370..4470
                      /label="dCas9"
                      /ApEinfo_revcolor=#b7e6d7
                      /ApEinfo_fwdcolor=#b7e6d7
     cloning junction 4799..4806
                      /label="USER junction 26"
                      /ApEinfo_revcolor=#75c6a9
                      /ApEinfo_fwdcolor=#75c6a9
     cloning junction 6343..6352
                      /label="USER junction 23"
                      /ApEinfo_revcolor=#d6b295
                      /ApEinfo_fwdcolor=#d6b295
     CDS              367..369
                      /label="from XTEN linker"
                      /ApEinfo_revcolor=#75c6a9
                      /ApEinfo_fwdcolor=#75c6a9
     CDS              4483..4737
                      /label="ugi"
                      /ApEinfo_revcolor=#9eafd2
                      /ApEinfo_fwdcolor=#9eafd2
     cloning junction 59..67
                      /label="USER junction 21"
                      /ApEinfo_revcolor=#c7b0e3
                      /ApEinfo_fwdcolor=#c7b0e3
     cloning junction 5674..5683
                      /label="USER junction 22"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     CDS              259..363
                      /label="Npu DnaE-C"
                      /ApEinfo_revcolor=#d6b295
                      /ApEinfo_fwdcolor=#d6b295
     cloning junction 4736..4743
                      /label="USER junction 15"
                      /ApEinfo_revcolor=#faac61
                      /ApEinfo_fwdcolor=#faac61
     misc_feature     complement(2..14)
                      /label="T-rich"
                      /ApEinfo_revcolor=#f8d3a9
                      /ApEinfo_fwdcolor=#f8d3a9
     terminator       1..45
                      /label="rrnB1 rho-independent transcriptional terminator"
                      /ApEinfo_revcolor=#d59687
                      /ApEinfo_fwdcolor=#d59687
     misc_feature     10..41
                      /label="stem loop"
                      /ApEinfo_revcolor=#ff9ccd
                      /ApEinfo_fwdcolor=#ff9ccd
     promoter         153..158
                      /label="proC -10"
                      /ApEinfo_revcolor=#d6b295
                      /ApEinfo_fwdcolor=#d6b295
     misc_feature     68..243
                      /label="insulated proC"
                      /ApEinfo_revcolor=#f58a5e
                      /ApEinfo_fwdcolor=#f58a5e
     prim_transcript  165..165
                      /label="+1 TSS"
                      /ApEinfo_revcolor=#75c6a9
                      /ApEinfo_fwdcolor=#75c6a9
     promoter         130..135
                      /label="-35"
                      /ApEinfo_revcolor=#ffef86
                      /ApEinfo_fwdcolor=#ffef86
     RBS              244..258
                      /label="SD8 RBS"
                      /ApEinfo_revcolor=#d59687
                      /ApEinfo_fwdcolor=#d59687
     terminator       4751..4792
                      /label="bidirectional terminator"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     misc_feature     complement(5621..5629)
                      /label="-10"
                      /ApEinfo_revcolor=#c7b0e3
                      /ApEinfo_fwdcolor=#c7b0e3
     misc_feature     complement(5623..5673)
                      /label="promoter (functional)"
                      /ApEinfo_revcolor=#75c6a9
                      /ApEinfo_fwdcolor=#75c6a9
     misc_feature     complement(5645..5650)
                      /label="-35"
                      /ApEinfo_revcolor=#c7b0e3
                      /ApEinfo_fwdcolor=#c7b0e3
     CDS              complement(4807..5622)
                      /label="aph (KanR)"
                      /ApEinfo_revcolor=#ffef86
                      /ApEinfo_fwdcolor=#ffef86
     promoter         complement(5870..5909)
                      /label="RNA I Promoter (0.96)"
                      /ApEinfo_revcolor=#9eafd2
                      /ApEinfo_fwdcolor=#9eafd2
     misc_RNA         5762..6342
                      /label="RNA II"
                      /ApEinfo_revcolor=#84b0dc
                      /ApEinfo_fwdcolor=#84b0dc
     promoter         5719..5758
                      /label="RNA II Promoter (0.92)"
                      /ApEinfo_revcolor=#b7e6d7
                      /ApEinfo_fwdcolor=#b7e6d7
     -35_signal       complement(5899..5904)
                      /label="-35"
                      /ApEinfo_revcolor=#d6b295
                      /ApEinfo_fwdcolor=#d6b295
     misc_feature     5774..5790
                      /label="hairpin"
                      /ApEinfo_revcolor=#ff9ccd
                      /ApEinfo_fwdcolor=#ff9ccd
     rep_origin       5716..6342
                      /label="p15A Ori"
                      /ApEinfo_revcolor=#f58a5e
                      /ApEinfo_fwdcolor=#f58a5e
     -10_signal       complement(5875..5880)
                      /label="-10"
                      /ApEinfo_revcolor=#84b0dc
                      /ApEinfo_fwdcolor=#84b0dc
     prim_transcript  complement(6270..6270)
                      /label="prim transcript"
                      /ApEinfo_revcolor=#c6c9d1
                      /ApEinfo_fwdcolor=#c6c9d1
     misc_RNA         complement(5765..5868)
                      /label="RNA I"
                      /ApEinfo_revcolor=#84b0dc
                      /ApEinfo_fwdcolor=#84b0dc
     -35_signal       5725..5730
                      /label="-35"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     -10_signal       5748..5753
                      /label="-10"
                      /ApEinfo_revcolor=#75c6a9
                      /ApEinfo_fwdcolor=#75c6a9
     misc_feature     6166..6184
                      /label="hairpin"
                      /ApEinfo_revcolor=#ff9ccd
                      /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttgctga ggagacttAg
       61 ggactcTcac agctaacacc acgtcgtccc tatctgctgc cctaggtcta tgagtggttg
      121 ctggataact ttacgggcat gcataaggct cgtatgatat attcagggag accacaacgg
      181 tttccctcta caaataattt tgtttaactt ttactagagt gggaccctac ctgcaggtgc
      241 agtaaggagg aaaaaaaaat gatcaagata gctacaagga agtatcttgg caaacaaaac
      301 gtttatgata ttggagtcga aagagatcac aactttgctc tgaagaacgg attcatagct
      361 tctAATtGTT TtaaCaaata ctcaataggc ttagctatcg gcacaaatag cgtcggatgg
      421 gcggtgatca ctgatgaata taaggttccg tctaaaaagt tcaaggttct gggaaataca
      481 gaccgccaca gtatcaaaaa aaatcttata ggggctcttt tatttgacag tggagagaca
      541 gcggaagcga ctcgtctcaa acggacagct cgtagaaggt atacacgtcg gaagaatcgt
      601 atttgttatc tacaggagat tttttcaaat gagatggcga aagtagatga tagtttcttt
      661 catcgacttg aagagtcttt tttggtggaa gaagacaaga agcatgaacg tcatcctatt
      721 tttggaaata tagtagatga agttgcttat catgagaaat atccaactat ctatcatctg
      781 cgaaaaaaat tggtagattc tactgataaa gcggatttgc gcttaatcta tttggcctta
      841 gcgcatatga ttaagtttcg tggtcatttt ttgattgagg gagatttaaa tcctgataat
      901 agtgatgtgg acaaactatt tatccagttg gtacaaacct acaatcaatt atttgaagaa
      961 aaccctatta acgcaagtgg agtagatgct aaagcgattc tttctgcacg attgagtaaa
     1021 tcaagacgat tagaaaatct cattgctcag ctccccggtg agaagaaaaa tggcttattt
     1081 gggaatctca ttgctttgtc attgggtttg acccctaatt ttaaatcaaa ttttgatttg
     1141 gcagaagatg ctaaattaca gctttcaaaa gatacttacg atgatgattt agataattta
     1201 ttggcgcaaa ttggagatca atatgctgat ttgtttttgg cagctaagaa tttatcagat
     1261 gctattttac tttcagatat cctaagagta aatactgaaa taactaaggc tcccctatca
     1321 gcttcaatga ttaaacgcta cgatgaacat catcaagact tgactctttt aaaagcttta
     1381 gttcgacaac aacttccaga aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga
     1441 tatgcaggtt atattgatgg gggagctagc caagaagaat tttataaatt tatcaaacca
     1501 attttagaaa aaatggatgg tactgaggaa ttattggtga aactaaatcg tgaagatttg
     1561 ctgcgcaagc aacggacctt tgacaacggc tctattcccc atcaaattca cttgggtgag
     1621 ctgcatgcta ttttgagaag acaagaagac ttttatccat ttttaaaaga caatcgtgag
     1681 aagattgaaa aaatcttgac ttttcgaatt ccttattatg ttggtccatt ggcgcgtggc
     1741 aatagtcgtt ttgcatggat gactcggaag tctgaagaaa caattacccc atggaatttt
     1801 gaagaagttg tcgataaagg tgcttcagct caatcattta ttgaacgcat gacaaacttt
     1861 gataaaaatc ttccaaatga aaaagtacta ccaaaacata gtttgcttta tgagtatttt
     1921 acggtttata acgaattgac aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca
     1981 tttctttcag gtgaacagaa gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa
     2041 gtaaccgtta agcaattaaa agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt
     2101 gaaatttcag gagttgaaga tagatttaat gcttcattag gtacctacca tgatttgcta
     2161 aaaattatta aagataaaga ttttttggat aatgaagaaa atgaagatat cttagaggat
     2221 attgttttaa cattgacctt atttgaagat agggagatga ttgaggaaag acttaaaaca
     2281 tatgctcacc tctttgatga taaggtgatg aaacagctta aacgtcgccg ttatactggt
     2341 tggggacgtt tgtctcgaaa attgattaat ggtattaggg ataagcaatc tggcaaaaca
     2401 atattagatt ttttgaaatc agatggtttt gccaatcgca attttatgca gctgatccat
     2461 gatgatagtt tgacatttaa agaagacatt caaaaagcac aagtgtctgg acaaggcgat
     2521 agtttacatg aacatattgc aaatttagct ggtagccctg ctattaaaaa aggtatttta
     2581 cagactgtaa aagttgttga tgaattggtc aaagtaatgg ggcggcataa gccagaaaat
     2641 atcgttattg aaatggcacg tgaaaatcag acaactcaaa agggccagaa aaattcgcga
     2701 gagcgtatga aacgaatcga agaaggtatc aaagaattag gaagtcagat tcttaaagag
     2761 catcctgttg aaaatactca attgcaaaat gaaaagctct atctctatta tctccaaaat
     2821 ggaagagaca tgtatgtgga ccaagaatta gatattaatc gtttaagtga ttatgatgtc
     2881 gatgccattg ttccacaaag tttccttaaa gacgattcaa tagacaataa ggtcttaacg
     2941 cgttctgata aaaatcgtgg taaatcggat aacgttccaa gtgaagaagt agtcaaaaag
     3001 atgaaaaact attggagaca acttctaaac gccaagttaa tcactcaacg taagtttgat
     3061 aatttaacga aagctgaacg tggaggtttg agtgaacttg ataaagctgg ttttatcaaa
     3121 cgccaattgg ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc
     3181 atgaatacta aatacgatga aaatgataaa cttattcgag aggttaaagt gattacctta
     3241 aaatctaaat tagtttctga cttccgaaaa gatttccaat tctataaagt acgtgagatt
     3301 aacaattacc atcatgccca tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt
     3361 aagaaatatc caaaacttga atcggagttt gtctatggtg attataaagt ttatgatgtt
     3421 cgtaaaatga ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa atatttcttt
     3481 tactctaata tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg agagattcgc
     3541 aaacgccctc taatcgaaac taatggggaa actggagaaa ttgtctggga taaagggcga
     3601 gattttgcca cagtgcgcaa agtattgtcc atgccccaag tcaatattgt caagaaaaca
     3661 gaagtacaga caggcggatt ctccaaggag tcaattttac caaaaagaaa ttcggacaag
     3721 cttattgctc gtaaaaaaga ctgggatcca aaaaaatatg gtggttttga tagtccaacg
     3781 gtagcttatt cagtcctagt ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa
     3841 tccgttaaag agttactagg gatcacaatt atggaaagaa gttcctttga aaaaaatccg
     3901 attgactttt tagaagctaa aggatataag gaagttaaaa aagacttaat cattaaacta
     3961 cctaaatata gtctttttga gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga
     4021 gaattacaaa aaggaaatga gctggctctg ccaagcaaat atgtgaattt tttatattta
     4081 gctagtcatt atgaaaagtt gaagggtagt ccagaagata acgaacaaaa acaattgttt
     4141 gtggagcagc ataagcatta tttagatgag attattgagc aaatcagtga attttctaag
     4201 cgtgttattt tagcagatgc caatttagat aaagttctta gtgcatataa caaacataga
     4261 gacaaaccaa tacgtgaaca agcagaaaat attattcatt tatttacgtt gacgaatctt
     4321 ggagctcccg ctgcttttaa atattttgat acaacaattg atcgtaaacg atatacgtct
     4381 acaaaagaag ttttagatgc cactcttatc catcaatcca tcactggtct ttatgaaaca
     4441 cgcattgatt tgagtcagct aggaggtgac AGCGGCGGTT CAATGACAAA TCTGTCGGAT
     4501 ATCATCGAAA AAGAAACCGG TAAACAGCTG GTTATCCAAG AAAGTATTTT AATGCTTCCC
     4561 GAAGAAGTCG AAGAGGTGAT TGGCAACAAA CCCGAAAGTG ATATTCTCGT GCATACTGCG
     4621 TATGATGAGT CGACCGATGA AAACGTCATG CTGCTGACCT CCGACGCGCC CGAGTATAAA
     4681 CCGTGGGCTT TGGTTATCCA GGATAGCAAC GGTGAAAATA AGATTAAAAT GTTAtaaggc
     4741 actcctcagc aaatataatg accctcttga taacccaaga gggcattttt taatgcccAt
     4801 ggcgtcttag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
     4861 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
     4921 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
     4981 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     5041 gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac
     5101 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
     5161 tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
     5221 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     5281 aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca
     5341 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     5401 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     5461 cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg
     5521 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     5581 tcgcggccta gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
     5641 gtttatgtaa gcagacagtt ttattgttca tgaAgagaaa ccTacggatc cttattaagg
     5701 agccccatac gatatatgat cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc
     5761 tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag gttctctgag ctaccaactc
     5821 tttgaaccga ggtaactggc ttggaggagc gcagtcacca aaacttgtcc tttcagttta
     5881 gccttaaccg gcgcatgact tcaagactaa ctcctctaaa tcaattacca gtggctgctg
     5941 ccagtggtgc ttttgcatgt ctttccgggt tggactcaag acgatagtta ccggataagg
     6001 cgcagcggtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct
     6061 acccggaact gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca
     6121 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccgccagg gggaaacgcc
     6181 tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga
     6241 tgcttgtcag gggggcggag cctatggaaa aacggctttg ccgcggccct ctcacttccc
     6301 tgttaagtat cttcctggca tcttccagga aatctccgcc ccAagaggac aTccggt
//