LOCUS       pBT100.110_CDA1-intein_ 2897 bp ds-DNA     circular     22-JAN-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     ORF             716..1024
                     /label="Npu DnaE N-term intein"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    15..46
                     /label="syn RBS 654k t.i.r. from proIII"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             671..715
                     /label="XTEN linker"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1025..1027
                     /label="unintentional duplication from cloning"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             47..670
                     /label="pmCDA1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     cloning junction 12..14
                     /label="AGT for SapI Golden Gate cloning"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     restriction enzyme recognition sequence 4..10
                     /label="SapI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     cloning junction 1..9
                     /label="USER junction 16"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     cloning junction 1028..1030
                     /label="GGC for SapI Golden Gate cloning"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     restriction enzyme recognition sequence complement(1032..1038)
                     /label="SapI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     cloning junction 1033..1040
                     /label="USER junction 13"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     original of replication 2068..2744
                     /label="pUC origin high copy"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     terminator      2748..2785
                     /label="predicted terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(2015..2023)
                     /label="-10"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(2017..2067)
                     /label="promoter (functional)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2039..2044)
                     /label="-35"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(1201..2016)
                     /label="aph (KanR)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 aatgctcttc tagtctacta cataaatcaa acataaggag gtaaccatga ccgacgcgga
       61 atacgttcgt atccacgaaa aactggacat ctacaccttc aaaaaacagt tcttcaacaa
      121 caaaaaatct gtttctcacc gttgctacgt tctgttcgaa ctgaaacgtc gtggtgaacg
      181 tcgtgcgtgc ttctggggtt acgcggttaa caaaccgcag tctggtaccg aacgtggtat
      241 ccacgcggaa atcttctcta tccgtaaagt tgaagaatac ctgcgtgaca acccgggtca
      301 gttcaccatc aactggtact cttcttggtc tccgtgcgcg gactgcgcgg aaaaaatcct
      361 ggaatggtac aaccaggaac tgcgtggtaa cggtcacacc ctgaaaatct gggcgtgcaa
      421 actgtactac gaaaaaaacg cgcgtaacca gatcggtctg tggaacctgc gtgacaacgg
      481 tgttggtctg aacgttatgg tttctgaaca ctaccagtgc tgccgtaaaa tcttcatcca
      541 gtcttctcac aaccagctga acgaaaaccg ttggctggaa aaaaccctga aacgtgcgga
      601 aaaacgtcgt tctgaactgt ctatcatgat ccaggttaaa atcctgcaca ccaccaaatc
      661 tccggcggtt tccggtagcg aaacaccggg gacttcagaa tcggccaccc cggAgtgcct
      721 gTcatacgaa accgagatac tgacagtaga atatggcctt ctgccaatcg ggaagattgt
      781 ggagaaacgg atagaatgca cagtttactc tgtcgataac aatggtaaca tttatactca
      841 gccagttgcc cagtggcacg accggggaga gcaggaagta ttcgaatact gtctggagga
      901 tggaagtctc attagggcca ctaaggacca caaatttatg acagtcgatg gccagatgct
      961 gcctatagac gaaatctttg agcgagagtt ggacctcatg cgagttgaca accttcctaa
     1021 ttaaggcggc tgaagagcct ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     1081 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     1141 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc
     1201 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
     1261 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
     1321 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
     1381 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
     1441 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
     1501 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
     1561 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     1621 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
     1681 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc
     1741 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
     1801 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
     1861 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac
     1921 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
     1981 cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
     2041 gtaagcagac agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact
     2101 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2161 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2221 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2281 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2341 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2401 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2461 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2521 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2581 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2641 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2701 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2761 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2821 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2881 gcgagtcagt gagcgag
//