LOCUS       pPEPY-term              3329 bp    DNA     circular SYN 08-JUN-2017
DEFINITION  product added to end of product, overlap trimmed
ACCESSION   pPEPY-term
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
FEATURES             Location/Qualifiers
     misc_feature    1..281
                     /gene="MCS"
                     /product="Multiple Cloning Site (BglFusion)"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     306..394
                     /label=TER_rrnB
                     /SECDrawAs="Label"
     misc_signal     399..450
                     /label=TER_rpsI
                     /SECDrawAs="Label"
     misc_signal     455..482
                     /label=TER_tufA
                     /SECDrawAs="Label"
     misc_feature    complement(503..1024)
                     /gene="Dwstr Insertion''"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    complement(1114..1772)
                     /gene="'oUC18"
                     /product="pUC18 origin of replication (multi copy)"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     1114..1772
                     /label=pUC18 origin
                     /SECDrawAs="Label"
     misc_feature    complement(1816..2340)
                     /gene="Upstr Insertion"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     complement(2351..2376)
                     /label=T_KanR
                     /product="Terminator: KanR"
                     /SECDrawAs="Label"
     CDS             complement(2414..3205)
                     /gene="kan"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="kan"
     misc_signal     complement(3206..3294)
                     /label=P_PF6
                     /product="Promoter: PF6"
                     /SECDrawAs="Label"
ORIGIN      
        1 ggtctccgat cgtgtactgc agaaaactag tcgcaaaaaa ccccgcttcg gcggggtttt
       61 ttcgctctag acggtgatca acacgctagc caggcatcaa ataaaacgaa aggctcagtc
      121 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      181 cactggctca ccttcgggtg ggcctttctg cgtttataga attcatgaga tctaaataaa
      241 taaggaggaa aattaatgcc ggatccctcc agtaactcga gaaagtgtaa gcaattctgc
      301 agtactagga cgccgccaag ccagcttaaa cccagctcaa tgagctgggt tttttgtttg
      361 ttaaaaatga agaagaaact gtgaagcgta tttatagcaa agcactcaaa agtttacctt
      421 atgggtgctt ttttcgtgct tttttgaaaa gacaaaaaaa agaaccttgc caagcaagat
      481 tcttttgata gcgcagttga gcagtgtctt tagtaaattt agggacttgt tttgtcatag
      541 ttttgatacc tgtattcgtt gtttcatgag catttgacgt ttcttttcat gtaggcattt
      601 caacaattag aatgatattt ttccgaggat gaacttatat attgtgactt tttagagtta
      661 tgatgcctct tgatttgact atcgctatca atttcatcca cttttacagt ttcatggttt
      721 tatgaaccga aacatctagg tacaggtttt agcatcaatt gattgttttt gagccatatg
      781 aagttgaaac gtggagatac ttttcttgtg ataacgccct attcaaagag gaataaccgt
      841 ttttggagag agttcagtac tatgtggcgc taaatactat atgtaaaaca tatattaaac
      901 aagatttaac taaggagaat tgtaaaaaca aaaatgagaa tttcatataa agtttattct
      961 aggagtatgt atcttgaata aaaattgtta tttaagttct gggggttatg atgaatgttt
     1021 ttatgcggcc gcgggataac gcaggaaaga acatgtgagc aaaaggcata aaaacattca
     1081 tcataacccc cagaacttaa ataacgcggc cgcgggataa cgcaggaaag aacatgtgag
     1141 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1201 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1261 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1321 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1381 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1441 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1501 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1561 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1621 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1681 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1741 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1801 ctacggggtg gtaccaaacc ttactaaagt atataattta ggcagtcaat tctgtatgat
     1861 aatgtttgta ctatcgtcga gtttttgctt gattgcctaa cgagataaat tttagaagag
     1921 ttttcggtgt gatttgacaa gtttctgact ggaaggatag ttacactagt attgtttatt
     1981 aaaatgagca gatagagaaa atttggaatc tatttgctta ttatcaatcg tttttttagc
     2041 ttatttgtct tagattggta tagaactgag ctatggatta atcttcttct atcttatcct
     2101 gttcctaaat atgttggtag aaaaatgaag cagttgttac gagtgtctgt attggaaaaa
     2161 aattttgtct atacaattct ttaaatgata tgaagactag gaatgccaaa acactgacat
     2221 atttcgttta aagcctatcc agtctcttca attgtatttg tagaaatgct atcagtaata
     2281 tattcgcgaa cactatgatt tacaacagtg tggtcgtaca tctttgtaac tttattatac
     2341 agcttctagc aaaaaactgg acggagtccc gtccagaaaa gtaagcactt gttattgcaa
     2401 taaaattagc ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
     2461 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
     2521 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
     2581 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
     2641 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
     2701 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
     2761 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
     2821 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
     2881 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
     2941 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
     3001 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
     3061 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
     3121 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
     3181 ccggtgatat tctcatttta gccattaatt ttcctcctta tttatttaga tccttgcatg
     3241 tatagttatt ataccagggg gacagtgcaa tgtcaagctg aaatcttcga gcagaagaac
     3301 cttgccaagc aagattcttt tgatagggt
//
