LOCUS       pPEPZ-Plac              4314 bp    DNA     circular SYN 29-JAN-2019
DEFINITION  pPEPZ-Plac rotated to position 3050
ACCESSION   pPEPZ-Plac
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Sequence has been copy-paste from Benching
            integration vector at SPD39_1735. Will remove most of the gene and 
            insert MCS+spec marker from pPEP1. 
            Contains pUC18 ori.
FEATURES             Location/Qualifiers
     misc_signal     75..96
                     /label=lacO
                     /product="lac operator sequence"
                     /SECDrawAs="Label"
     misc_feature    75..173
                     /gene="Plac"
                     /product="PL8-2 promoter"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     120
                     /label=-35
                     /product="-35 box (consensus: TTGACA)"
                     /SECDrawAs="Label"
     misc_signal     145
                     /label=-10
                     /product="-10 box (consensus: TATAAT)"
                     /SECDrawAs="Label"
     misc_signal     153..172
                     /label=lacO
                     /product="lac operator sequence"
                     /SECDrawAs="Label"
     misc_signal     228..267
                     /label=T_B1006 ->|<-
                     /product="Terminator: B1006 (modified) (synthetic;
                     bidirectional)"
                     /SECDrawAs="Label"
     misc_feature    267..1265
                     /gene="ISD-SPV39_1734"
                     /product="Integration Site Down (S. pneumoniae D39
                     1727381-1728378-SPV39_1734)"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1275..1970
                     /gene="oUC18"
                     /product="pUC18 origin of replication (multi copy)"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1360..1362
                     /label=after sequencing
                     /product="acc in the original file"
                     /SECDrawAs="Info only"
     misc_feature    1977..2980
                     /gene="ISU-SPV39_1736"
                     /product="Integration Site Up (S. pneumoniae D390
                     172879-172977-SPV39_1736)"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     complement(2991..3016)
                     /label=T_SpR |<-
                     /product="Terminator: SpR"
                     /SECDrawAs="Label"
     CDS             complement(3051..3833)
                     /gene="SpcR"
                     /product="Spectinomycin Resistance (Streptomycin
                     3''-adenylyltransferase)"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    3998..4053
                     /label=new sequence
                     /product="change from original sequence"
                     /SECDrawAs="Info only"
     misc_signal     4063..4114
                     /label=T_rpsI ->|
                     /product="Terminator: S. pneumoniae rpsI (forward)"
                     /SECDrawAs="Label"
     misc_signal     complement(4119..4146)
                     /label=T_tuf |<-
                     /product="Terminator: S. pneumoniae tuf (reverse)"
                     /SECDrawAs="Label"
     misc_signal     4190..4223
                     /label=T_B1002 ->|<-
                     /product="Terminator: B1002 (synthetic; bidirectional)"
                     /SECDrawAs="Label"
     misc_signal     join(4248..4314,1..62)
                     /label=T_B0015 ->|->|
                     /product="Terminator: B0015 (E. coli rrnBT1 + T7TE; double
                     forward)"
                     /SECDrawAs="Label"
ORIGIN      
        1 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
       61 tagaattcac tagtaattgt gaggcggata acaattctca ctcattctac agtttattct
      121 tgacattgca ctgtccccct ggtataataa ctaattgtga gcgctcacaa ttaagatcta
      181 aataaataag gaggaaaaat aatgccggat ccctccagta actcgagaaa aaaaaaccgc
      241 gcccctgaca gggcgcggtt tttttttcgt aatttattac tgaacaagag ggcaatcata
      301 tctggaataa tatgcttaaa aagagaagga aaattggtgc aaattcattt tcagactatc
      361 ttcgtggaag tattcatcaa aatagacaaa aataaatttg gataaatcga actcactatt
      421 caggaggcat atgagcaatt cgaaaaagaa aagtgtcaaa ttgagcctat aggagtagaa
      481 gtgaaatagt aagtcctgca tagtggatga gagaaaagtt ctccttgaag ttttcctgaa
      541 ctatcagtcg catgtcaaac gatatgtagg gtaatgtgag aggggatagc gagtagtttt
      601 tggttatttt atcaaaaaac ttatatttta ttataccgaa tgataaaata taataaaaat
      661 gatagaataa ggaaaaaaca tgaatgtcaa aaagataatg tcaatttttc aatcctttta
      721 tgttgatgtc agtattgagg aactgacttt gactttacca atcagttttg taaaaaggtt
      781 tgagtatact caaatgactt ttcataagga atcattttta ttgattaaag aaaagagaag
      841 ggggagtttg agttcatttg ttactcaggc tcgcactatg ggtgaaaaag ccaatatgga
      901 tgttgttttg gtgttttcga agttatcaga cagtgaaaaa aagcaattac ttcaagctag
      961 agttccgttt gtagacttta agggaaacct cttcttccct ccattgggac tagtactcaa
     1021 tgcgaatgat actgaagtcc ctaaggaatt aacacctagc gaacaattaa cgtggattgc
     1081 ctttttattg acaaaaggtc aaaaagtagt agatgttgat ttgctttcac aagtcactgg
     1141 acttccaaac tcaacaattt ataggtgttt gaggactttt aaagctttat attggttaaa
     1201 caagcaaaat aagctttaca catatacggt gtcaaagaaa gaattattct taaaatccgt
     1261 gtcatgttgg taccaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1321 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgctg gtagcggtgg tttgtttgcc
     1381 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1441 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1501 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1561 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1621 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1681 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1741 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1801 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1861 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1921 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc gcggccgcca
     1981 ataaattgct tccttgtttt gtcataattt ttttatttaa aaaattatga caaaagtgtg
     2041 ctattctttt tatgagaggt gtatgaatat gataaatgta tgtgataaat gtatgtgatg
     2101 ttggaaaaag aataaaagaa cttagaatat cttcaaatct tactcaagat aagattgctg
     2161 aatatttgtc tttatatttg tctttgaatc aaagcatgat tgccaaaatg gaaaaaggtg
     2221 aaaggaatat cacgaatgga tttaagtaat aaagcttcaa atcttagaaa aaagttggga
     2281 gctgatggtg aagcgccgat agatattttt aaattggtac aaaagataga aaatttgacg
     2341 ctggtatttt atggactcgg aaagaatatt agcggagtct gttataaagg aactcagttc
     2401 agtctcattg cagtcaattc agacatgtca ttaggaaggt aaagattttc tttagcacat
     2461 gaactgtatc atctttatta tgatgaggtg aagaagagtt cagtcagtct tatcttgatt
     2521 ggtgaaggag atgaaactga aagaaaagcg gatcagcttg cttcttattt tttaattttc
     2581 ccatcttcac tgtataggat ggttgaggaa atcagagaaa atgccaatag aactcatctt
     2641 gaagtagaag atattataaa attgggtcag ttttatggta tcagtcataa agctatgtta
     2701 tatagattga ggaatgatgg ataccttgat gcagaagaaa ttaaaaatat ggatattagt
     2761 gttatagaga cagcttcaag attaggctat gatacaagtt tatatcgtcc tttgtcagaa
     2821 agtaaaaagg aaatggtatt aggataatat attaattcaa ctgaacaact tttagaaaat
     2881 aacagaattt cgcaagggaa gtatgaggaa ctgttactag atgctttcag atatgatatt
     2941 gtatatgggc tagatgaaga ggggggagtt gtcgttgaaa agcttctagc aaaaaactgg
     3001 acggagtccc gtccagaaaa gtaagcactt gttattgcaa taaaattagc ctaattgaga
     3061 gaagtttcta tagaattttt catatactta acgagtgctt tcacctttga atatagtcct
     3121 tcccacttat catcacactc tccccgatag ccttttctag ctatatccag taaagttaca
     3181 tgctctttag gtaaaagagg tatagcccat tcagcagcga catctttcga ggtaatttca
     3241 ccagtagtca ctgtttgcca cattcgagct agggttaaaa ttacattacg ctcatcacct
     3301 tttatcccct caattagttc tggcaaagaa tccttaattg ctcttcgaat atctgtcaaa
     3361 ggtacggaga caagtatact tgaagaatca ggaccaaata gagaaatact attctttctt
     3421 gcttgtgcta aaacaatagc caaatcagga tcatagcttg gttcctgaat ttgtccattc
     3481 tcaaattcac ccctgagcca ctcaccgtat ataaattctc tttttggagg atattgccaa
     3541 gggacaactt cactcctatt tataaccgta acttcaagtg gtctaacaga atccgtattt
     3601 ccaatccttc ctgatatagt cattagtctt tctgttagtt tttttcgagt taattgaggt
     3661 aaactatgat tcacgacgac tagaacatct acatcgctgt taatgcgtaa accaccattt
     3721 actgctgaac caaatagata tactccaact attgaacttc caaataaatc ttttacgatt
     3781 tttaatgttt gaatcgcttg atttggtatt tttccgttaa tcaaattgct catgatttca
     3841 cctcgttgat tatgttcata taaagtttat attgatactc aatttactta ccctagattg
     3901 gacatatact taaattactg ttcaataaag ctgaccgtta gcgtttaagt acatcctttc
     3961 acaatttgtc tacagaacag tactggctat tatatcagcc ttcttattca atgtcaagaa
     4021 taaactgttc tatgtaaacc tattcattgt tttgcatgcg gcaaagcact caaaagttta
     4081 ccttatgggt gcttttttcg tgcttttttg aaaagacaaa aaaaagaacc ttgccaagca
     4141 agattctttt gatagggtgg tctccgatcg tgtactgcag aaaactagtc gcaaaaaacc
     4201 ccgcttcggc ggggtttttt cgctctagac ggtgatcaac acgctagcca ggcatcaaat
     4261 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtc
//
