LOCUS       Exported                2800 bp ds-DNA     circular SYN 05-FEB-2019
DEFINITION  Entry vector containing MS2-NLS (no stop codon) coding sequence with
            GreenGate C-D flanking sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Joanne Lee
  TITLE     Direct Submission
  JOURNAL   Exported Feb 5, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    380..385
                     /label=BsaI recognition site
     CDS             393..872
                     /codon_start=1
                     /product="Ath codon-optimised sequence for MS2 
                     bacteriophage coat protein linked to nuclear localisation 
                     signal (no stop codon)"
                     /label=MS2-NLS
                     /translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKV
                     TCSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCEL
                     IVKAMQGLLKDGNPIPSAIAANSGIYSAGGGGSGGGGSGGGGSGPKKKRKVAAAGS"
     misc_feature    complement(878..883)
                     /label=BsaI recognition site
     CDS             1875..2672
                     /codon_start=1
                     /label=KanR
                     /translation="LLEKFIQQTIKRNTLAIRCRNAIQHQKTIRPFAAQFFRNITGGQR
                     NILITIRHAQTAAINKAAKTAIFHHNVRQARITMGHHQIFAIRHARFQTRKQLCRCQAL
                     MFFIQIILIHQARFHTGTRTFNTMFRLMIKRTGRRVQGMQTTHGIRHNAHFFCRRQMAR
                     QQILTRHFAQQQPITARFGHHIQHRRTRNTGGGQPAQTRRFILQLVQRTAQIGFHKQHR
                     TTLRAQTKHRRIRAANGLLRPIIAKQTFHPRCRATRMQAILFNH"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcata gtgaagcttg gtctcaggct ccatggcttc taattttact caatttgttt
      421 tggttgataa tggaggaact ggagatgtta ctgttgctcc ttctaatttt gctaatggag
      481 ttgctgaatg gatttcttct aattcaagat ctcaggctta taaggttact tgttctgtta
      541 gacaatcttc tgctcaaaag agaaagtata ctattaaggt tgaagttcct aaggttgcta
      601 ctcaaactgt tggaggagtt gaattgcctg ttgctgcttg gagatcttat ttgaatatgg
      661 aattgactat tcctattttt gctactaatt ctgattgtga attgattgtt aaggctatgc
      721 aaggattgtt gaaggatgga aatcctattc cttctgctat tgctgctaat tctggaattt
      781 attctgctgg aggaggagga tctggaggag gaggatctgg aggaggagga tctggaccta
      841 agaagaagag aaaggttgct gctgctggat cttcagtgag accgaattct cctgggcctc
      901 atgggccttc ctttcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      961 acatggtcat agctgtttcc ttgcgtattg ggcgctctcc gcttcctcgc tcactgactc
     1021 gctgcgctcg gtcgttcggg taaagcctgg ggtgcctaat gagcaaaagg ccagcaaaag
     1081 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1141 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1201 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1261 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1321 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1381 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1441 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1501 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1561 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1621 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1681 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1741 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1801 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1861 acttggtctg acagttatta gaaaaattca tccagcagac gataaaacgc aatacgctgg
     1921 ctatccggtg ccgcaatgcc atacagcacc agaaaacgat ccgcccattc gccgcccagt
     1981 tcttccgcaa tatcacgggt ggccagcgca atatcctgat aacgatccgc cacgcccaga
     2041 cggccgcaat caataaagcc gctaaaacgg ccattttcca ccataatgtt cggcaggcac
     2101 gcatcaccat gggtcaccac cagatcttcg ccatccggca tgctcgcttt cagacgcgca
     2161 aacagctctg ccggtgccag gccctgatgt tcttcatcca gatcatcctg atccaccagg
     2221 cccgcttcca tacgggtacg cgcacgttca atacgatgtt tcgcctgatg atcaaacgga
     2281 caggtcgccg ggtccagggt atgcagacga cgcatggcat ccgccataat gctcactttt
     2341 tctgccggcg ccagatggct agacagcaga tcctgacccg gcacttcgcc cagcagcagc
     2401 caatcacggc ccgcttcggt caccacatcc agcaccgccg cacacggaac accggtggtg
     2461 gccagccagc tcagacgcgc cgcttcatcc tgcagctcgt tcagcgcacc gctcagatcg
     2521 gttttcacaa acagcaccgg acgaccctgc gcgctcagac gaaacaccgc cgcatcagag
     2581 cagccaatgg tctgctgcgc ccaatcatag ccaaacagac gttccaccca cgctgccggg
     2641 ctacccgcat gcaggccatc ctgttcaatc atactcttcc tttttcaata ttattgaagc
     2701 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2761 caaatagggg ttccgcgcac atttccccga aaagtgccac
//
