LOCUS       Exported                4591 bp ds-DNA     circular SYN 05-FEB-2019
DEFINITION  Entry vector containing p300 core domain coding sequence with 
            GreenGate D-E flanking sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Joanne Lee
  TITLE     Direct Submission
  JOURNAL   Exported Feb 5, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    442..447
                     /label=BsaI recognition site
     CDS             455..2308
                     /codon_start=1
                     /product="Ath codon-optimised sequence for core domain of 
                     Homo sapiens p300 H3K27 histone acetyltransferase"
                     /label=p300 core domain
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     misc_feature    complement(2317..2322)
                     /label=BsaI recognition site
     CDS             complement(3531..4391)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tatcggatcc cggtctcatc aggtattttt aagccagagg agcttagaca
      481 agcacttatg cctactcttg aggctcttta cagacaagat cctgaatctc ttccattcag
      541 acagcctgtg gaccctcagc ttcttggtat tcctgactac ttcgatattg tgaagtctcc
      601 tatggatctt tctacaatta aaagaaaact tgacacagga caataccaag aaccttggca
      661 atacgttgat gacatctggc ttatgtttaa caatgcttgg ctttacaaca gaaagacttc
      721 tagagtttac aaatattgct ctaaactttc tgaagtgttc gaacaagaaa ttgatccagt
      781 gatgcaatct cttggttact gttgcggtag aaaacttgag ttctctcctc aaactctttg
      841 ctgctatgga aagcaacttt gtactattcc tagagatgca acatactatt cttatcaaaa
      901 cagataccat ttctgtgaaa aatgctttaa cgaaatccag ggtgaatctg tttctcttgg
      961 agacgaccct tctcagcctc agacaacaat caataaagag cagttctcta agagaaagaa
     1021 cgatactctt gaccctgagc ttttcgtgga gtgtactgag tgcggtagaa agatgcatca
     1081 gatttgtgtt cttcatcacg agatcatttg gccagctggt tttgtttgcg atggttgtct
     1141 taagaagtct gcaagaacta gaaaagagaa taagttctct gcaaagagac ttccttctac
     1201 tagacttgga actttccttg aaaacagagt gaacgatttc cttagaagac agaatcatcc
     1261 tgagtctgga gaggtgacag tgagagtggt tcatgcatct gataaaactg ttgaggttaa
     1321 acctggaatg aaagctagat tcgtggactc tggagagatg gcagaatctt ttccatacag
     1381 aacaaaagct ctttttgcat tcgaagagat tgatggtgtt gatctttgtt tcttcggtat
     1441 gcacgtgcaa gagtatggat ctgactgccc tccaccaaat caaagaagag tttacatttc
     1501 ttatcttgat tctgttcact tttttagacc aaaatgcctt agaacagcag tgtaccacga
     1561 gattcttatt ggttatcttg agtatgtgaa aaaacttggt tacacaactg gacatatttg
     1621 ggcttgccca ccttctgaag gtgatgatta tatttttcat tgccaccctc cagaccaaaa
     1681 gattcctaaa cctaaaagac ttcaagagtg gtacaaaaag atgcttgata aagcagtgtc
     1741 tgagagaatt gtgcacgact acaaggacat ttttaaacag gctacagagg atagacttac
     1801 ttctgcaaaa gagcttcctt attttgaagg agatttttgg cctaatgttc ttgaggagtc
     1861 tattaaggaa cttgagcagg aggaagaaga gagaaaaaga gaagagaaca catctaatga
     1921 gtctacagac gttactaaag gtgattctaa aaacgctaaa aagaaaaaca ataaaaaaac
     1981 ttctaaaaat aaatcttctc tttctagagg taacaaaaaa aaacctggaa tgccaaacgt
     2041 ttctaatgac ctttctcaaa aactttacgc aacaatggaa aaacataaag aagttttctt
     2101 cgtgatcaga cttattgctg gacctgctgc taattctctt cctccaatcg ttgacccaga
     2161 ccctcttatt ccttgtgatc ttatggatgg aagagacgca tttcttactc ttgcaagaga
     2221 caaacatctt gaattttctt ctcttagaag agcacagtgg tctacaatgt gtatgcttgt
     2281 tgagcttcac acacaatctc aagactagta actgcagaga ccgggcccgt cgactgcaga
     2341 ggcctgcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2401 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2461 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2521 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2581 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2641 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2701 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2761 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2821 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2881 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2941 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3001 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3061 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3121 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3181 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3241 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3301 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3361 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3421 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3481 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3541 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3601 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3661 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3721 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3781 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3841 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3901 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3961 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4021 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4081 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4141 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4201 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4261 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4321 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4381 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4441 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4501 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     4561 aaaaataggc gtatcacgag gccctttcgt c
//
