LOCUS       Exported                3335 bp ds-DNA     circular SYN 05-FEB-2019
DEFINITION  Entry vector containing G9a SET domain coding sequence with 
            GreenGate D-E flanking sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Joanne Lee
  TITLE     Direct Submission
  JOURNAL   Exported Feb 5, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1178..2038)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    2451..2456
                     /label=BsaI recognition site
     CDS             2464..3321
                     /codon_start=1
                     /product="Ath codon-optimised sequence of SET catalytic 
                     domain from Homo sapiens G9a H3K9 histone 
                     methyltransferase"
                     /label=G9a SET domain
                     /translation="MAGENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQ
                     HCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRSCKNRV
                     VQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLD
                     NKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEE
                     LGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPDLSSLPPI
                     NTTG"
     misc_feature    complement(3330..3335)
                     /label=BsaI recognition site
ORIGIN
        1 acctgctttt gctcgcttgg atccgaattc ctgtgtgaaa ttgttatccg ctcacaattc
       61 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      121 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      181 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      241 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      301 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      361 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      421 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      481 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      541 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      601 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      661 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      721 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      781 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      841 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
      901 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      961 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1021 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1081 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1141 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1201 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1261 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgac
     1321 tcccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1381 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1441 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     1501 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1561 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     1621 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     1681 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     1741 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     1801 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     1861 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     1921 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     1981 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2041 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     2101 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     2161 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     2221 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     2281 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     2341 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     2401 agattgtact gagagtgcac caattgggta ccgagctcgc ggccgcaagc ggtctcatca
     2461 ggtatggctg gtgagaatgt gccaatccct tgtgtcaatg gggtagatgg agaaccttgt
     2521 cctgaagatt acaagtacat atcggaaaat tgcgagacaa gcacgatgaa tatcgatcgg
     2581 aacattactc atctccaaca ctgtacatgt gttgatgact gctcatcttc aaactgtctt
     2641 tgcggtcaat tgtcaatcag atgttggtac gataaagacg gaagattgct tcaggagttt
     2701 aacaaaatcg aaccacctct catctttgag tgtaatcaag cctgcagttg ttggagatct
     2761 tgcaagaatc gagttgtcca aagtggcatc aaagttcgtt tacagctcta tcgtactgct
     2821 aaaatgggat ggggtgtaag agcacttcag accattccac aaggtacctt catttgcgag
     2881 tatgtgggag agctaatttc cgacgctgaa gcagacgtta gagaggatga tagctacttg
     2941 tttgacctcg ataacaagga tggcgaagtc tactgcatag atgctcgata ttatgggaac
     3001 atttctaggt tcataaacca tctgtgtgat cccaatatca ttccagtgag agttttcatg
     3061 ttacaccagg atttacggtt tcctaggatt gccttctttt ctagcagaga tatacgtaca
     3121 ggagaggaat tggggtttga ctatggtgac agattctggg atattaagtc caagtatttc
     3181 acttgtcagt gtggatctga gaaatgcaag catagtgctg aagcgattgc gttggaacaa
     3241 tctaggctag caaggcttga tccgcatcct gaactgcttc ccgatctttc gtcactacca
     3301 ccgataaaca cgactggtta gtaactgcag agacc
//
