LOCUS       Exported                9657 bp ds-DNA     circular SYN 03-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 4, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9657
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     21..37
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(71..87)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..138)
                     /label=Recomb OH 1
     promoter        complement(113..131)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(141..157)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      302..757
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     624..643
                     /label=oRH4736
     promoter        811..1050
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1051..5229
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(5797..5816)
                     /label=oRH4872
     primer_bind     5819..5867
                     /label=oRH5045
     CDS             complement(5996..6655)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(6656..6842)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(6872..6921)
                     /label=oRH5046
     promoter        6924..7028
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7029..7889
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8060..8648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        8972..9002
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9010..9026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9034..9050
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9071..9089
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     9118..9145
                     /label=oRH4866
     misc_feature    9144..9647
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     complement(join(9638..9657,1..20))
                     /label=oRH4867
     primer_bind     join(9649..9657,1..21)
                     /label=Recomb OH 2
ORIGIN
        1 agcgctaaga gggcggccgc tctagaacta gtggatcccc cgggctgcag gaattcgata
       61 tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccaattc gccctatagt
      121 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      181 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      241 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      301 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      361 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      421 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      481 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      541 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      601 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      661 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      721 aacgcgaatt ttaacaaaat attaacgttt acaatttcct gatgcggtat tttctcctta
      781 cgcatctgtg cggtatttca caccgcatat aattccactt gcaattacat aaaaaattcc
      841 ggcggttttt cgcgtgtgac tcaatgtcga aatacctgcc taatgaacat gaacatcgcc
      901 caaatgtatt tgaagacccg ctgggagaag ttcaagatat ataagtaaca agcagccaat
      961 agtataaaaa aaaatctgag tttattacct ttcctggaat ttcagtgaaa aactgctaat
     1021 tatagagaga tatcacagag ttactcacta atgactaacg aaaaggtctg gatagagaag
     1081 ttggataatc caactctttc agtgttacca catgactttt tacgcccaca acaagaacct
     1141 tatacgaaac aagctacata ttcgttacag ctacctcagc tcgatgtgcc tcatgatagt
     1201 ttttctaaca aatacgctgt cgctttgagt gtatgggctg cattgatata tagagtaacc
     1261 ggtgacgatg atattgttct ttatattgcg aataacaaaa tcttaagatt caatattcaa
     1321 ccaacgtggt catttaatga gctgtattct acaattaaca atgagttgaa caagctcaat
     1381 tctattgagg ccaatttttc ctttgacgag ctagctgaaa aaattcaaag ttgccaagat
     1441 ctggaaagga cccctcagtt gttccgtttg gcctttttgg aaaaccaaga tttcaaatta
     1501 gacgagttca agcatcattt agtggacttt gctttgaatt tggataccag taataatgcg
     1561 catgttttga acttaattta taacagctta ctgtattcga atgaaagagt aaccattgtt
     1621 gcggaccaat ttactcaata tttgactgct gcgctaagcg atccatccaa ttgcataact
     1681 aaaatctctc tgatcaccgc atcatccaag gatagtttac ctgatccaac taagaacttg
     1741 ggctggtgcg atttcgtggg gtgtattcac gacattttcc aggacaatgc tgaagccttc
     1801 ccagagagaa cctgtgttgt ggagactcca acactaaatt ccgacaagtc ccgttctttc
     1861 acttatcgcg acatcaaccg cacttctaac atagttgccc attatttgat taaaacaggt
     1921 atcaaaagag gtgatgtagt gatgatctat tcttctaggg gtgtggattt gatggtatgt
     1981 gtgatgggtg tcttgaaagc cggcgcaacc ttttcagtta tcgaccctgc atatccccca
     2041 gccagacaaa ccatttactt aggtgttgct aaaccacgtg ggttgattgt tattagagct
     2101 gctggacaat tggatcaact agtagaagat tacatcaatg atgaattgga gattgtttca
     2161 agaatcaatt ccatcgctat tcaagaaaat ggtaccattg aaggtggcaa attggacaat
     2221 ggcgaggatg ttttggctcc atatgatcac tacaaagaca ccagaacagg tgttgtagtt
     2281 ggaccagatt ccaacccaac cctatctttc acatctggtt ccgaaggtat tcctaagggt
     2341 gttcttggta gacatttttc cttggcttat tatttcaatt ggatgtccaa aaggttcaac
     2401 ttaacagaaa atgataaatt cacaatgctg agcggtattg cacatgatcc aattcaaaga
     2461 gatatgttta caccattatt tttaggtgcc caattgtatg tccctactca agatgatatt
     2521 ggtacaccgg gccgtttagc ggaatggatg agtaagtatg gttgcacagt tacccattta
     2581 acacctgcca tgggtcaatt acttactgcc caagctacta caccattccc taagttacat
     2641 catgcgttct ttgtgggtga cattttaaca aaacgtgatt gtctgaggtt acaaaccttg
     2701 gcagaaaatt gccgtattgt taatatgtac ggtaccactg aaacacagcg tgcagtttct
     2761 tatttcgaag ttaaatcaaa aaatgacgat ccaaactttt tgaaaaaatt gaaagatgtc
     2821 atgcctgctg gtaaaggtat gttgaacgtt cagctactag ttgttaacag gaacgatcgt
     2881 actcaaatat gtggtattgg cgaaataggt gagatttatg ttcgtgcagg tggtttggcc
     2941 gaaggttata gaggattacc agaattgaat aaagaaaaat ttgtgaacaa ctggtttgtt
     3001 gaaaaagatc actggaatta tttggataag gataatggtg aaccttggag acaattctgg
     3061 ttaggtccaa gagatagatt gtacagaacg ggtgatttag gtcgttatct accaaacggt
     3121 gactgtgaat gttgcggtag ggctgatgat caagttaaaa ttcgtgggtt cagaatcgaa
     3181 ttaggagaaa tagatacgca catttcccaa catccattgg taagagaaaa cattacttta
     3241 gttcgcaaaa atgccgacaa tgagccaaca ttgatcacat ttatggtccc aagatttgac
     3301 aagccagatg acttgtctaa gttccaaagt gatgttccaa aggaggttga aactgaccct
     3361 atagttaagg gcttaatcgg ttaccatctt ttatccaagg acatcaggac tttcttaaag
     3421 aaaagattgg ctagctatgc tatgccttcc ttgattgtgg ttatggataa actaccattg
     3481 aatccaaatg gtaaagttga taagcctaaa cttcaattcc caactcccaa gcaattaaat
     3541 ttggtagctg aaaatacagt ttctgaaact gacgactctc agtttaccaa tgttgagcgc
     3601 gaggttagag acttatggtt aagtatatta cctaccaagc cagcatctgt atcaccagat
     3661 gattcgtttt tcgatttagg tggtcattct atcttggcta ccaaaatgat ttttacctta
     3721 aagaaaaagc tgcaagttga tttaccattg ggcacaattt tcaagtatcc aacgataaag
     3781 gcctttgccg cggaaattga cagaattaaa tcatcgggtg gatcatctca aggtgaggtc
     3841 gtcgaaaatg tcactgcaaa ttatgcggaa gacgccaaga aattggttga gacgctacca
     3901 agttcgtacc cctctcgaga atattttgtt gaacctaata gtgccgaagg aaaaacaaca
     3961 attaatgtgt ttgttaccgg tgtcacagga tttctgggct cctacatcct tgcagatttg
     4021 ttaggacgtt ctccaaagaa ctacagtttc aaagtgtttg cccacgtcag ggccaaggat
     4081 gaagaagctg catttgcaag attacaaaag gcaggtatca cctatggtac ttggaacgaa
     4141 aaatttgcct caaatattaa agttgtatta ggcgatttat ctaaaagcca atttggtctt
     4201 tcagatgaga agtggatgga tttggcaaac acagttgata taattatcca taatggtgcg
     4261 ttagttcact gggtttatcc atatgccaaa ttgagggatc caaatgttat ttcaactatc
     4321 aatgttatga gcttagccgc cgtcggcaag ccaaagttct ttgactttgt ttcctccact
     4381 tctactcttg acactgaata ctactttaat ttgtcagata aacttgttag cgaagggaag
     4441 ccaggcattt tagaatcaga cgatttaatg aactctgcaa gcgggctcac tggtggatat
     4501 ggtcagtcca aatgggctgc tgagtacatc attagacgtg caggtgaaag gggcctacgt
     4561 gggtgtattg tcagaccagg ttacgtaaca ggtgcctctg ccaatggttc ttcaaacaca
     4621 gatgatttct tattgagatt tttgaaaggt tcagtccaat taggtaagat tccagatatc
     4681 gaaaattccg tgaatatggt tccagtagat catgttgctc gtgttgttgt tgctacgtct
     4741 ttgaatcctc ccaaagaaaa tgaattggcc gttgctcaag taacgggtca cccaagaata
     4801 ttattcaaag actacttgta tactttacac gattatggtt acgatgtcga aatcgaaagc
     4861 tattctaaat ggaagaaatc attggaggcg tctgttattg acaggaatga agaaaatgcg
     4921 ttgtatcctt tgctacacat ggtcttagac aacttacctg aaagtaccaa agctccggaa
     4981 ctagacgata ggaacgccgt ggcatcttta aagaaagaca ccgcatggac aggtgttgat
     5041 tggtctaatg gaataggtgt tactccagaa gaggttggta tatatattgc atttttaaac
     5101 aaggttggat ttttacctcc accaactcat aatgacaaac ttccactgcc aagtatagaa
     5161 ctaactcaag cgcaaataag tctagttgct tcaggtgctg gtgctcgtgg aagctccgca
     5221 gcagcttaag gttgagcatt acgtatgata tgtccatgta caataattaa atatgaatta
     5281 ggagaaagac ttagcttctt ttcgggtgat gtcacttaaa aactccgaga ataatatata
     5341 ataagagaat aaaatattag ttattgaata agaactgtaa atcagctggc gttagtctgc
     5401 taatggcagc ttcatcttgg tttattgtag catgaatcat atttgccttt ttttcctgta
     5461 attcaatgat tcttgcttct atactatcct caatgcaaaa ccttgtgatc ttcacaggtc
     5521 gatactgacc aattctatga actctatcac cactttgcca ttcaacacta gggttccacc
     5581 atgggtctaa aatgaatact tgcgaagcta tatggtgcac tctcagtaca atctgctctg
     5641 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5701 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5761 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5821 tatttttata ggttaatgtc atgatgcctc gttcagaatg acacgtatag aatgatgcat
     5881 taccttgtca tcttcagtat catactgttc gtatacatac ttactgacat tcataggtat
     5941 acatatatac acatgtatat atatcgtatg ctgcagcttt aaataatcgg tgtcactaca
     6001 taagaacacc tttggtggag ggaacatcgt tggtaccatt gggcgaggtg gcttctctta
     6061 tggcaaccgc aagagccttg aacgcactct cactacggtg atgatcattc ttgcctcgca
     6121 gacaatcaac gtggagggta attctgctag cctctgcaaa gctttcaaga aaatgcggga
     6181 tcatctcgca agagagatct cctactttct ccctttgcaa accaagttcg acaactgcgt
     6241 acggcctgtt cgaaagatct accaccgctc tggaaagtgc ctcatccaaa ggcgcaaatc
     6301 ctgatccaaa cctttttact ccacgcgcca gtagggcctc tttaaaagct tgaccgagag
     6361 caatcccgca gtcttcagtg gtgtgatggt cgtctatgtg taagtcacca atgcactcaa
     6421 cgattagcga ccagccggaa tgcttggcca gagcatgtat catatggtcc agaaacccta
     6481 tacctgtgtg gacgttaatc acttgcgatt gtgtggcctg ttctgctact gcttctgcct
     6541 ctttttctgg gaagatcgag tgctctatcg ctaggggacc accctttaaa gagatcgcaa
     6601 tctgaatctt ggtttcattt gtaatacgct ttactagggc tttctgctct gtcatctttg
     6661 ccttcgttta tcttgcctgc tcatttttta gtatattctt cgaagaaatc acattacttt
     6721 atataatgta taattcatta tgtgataatg ccaatcgcta agaaaaaaaa agagtcatcc
     6781 gctaggggaa aaaaaaaaat gaaaatcatt accgaggcat aaaaaaatat agagtgtact
     6841 agaggaggcc aagagtaata gaaaaagaaa attgcgggaa aggactgtgt tagacgtcag
     6901 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6961 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7021 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7081 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7141 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7201 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7261 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7321 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7381 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7441 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7501 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7561 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7621 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7681 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7741 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7801 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7861 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7921 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7981 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8041 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8101 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8161 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     8221 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8281 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8341 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8401 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8461 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8521 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8581 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8641 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8701 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8761 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8821 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8881 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     8941 tgagttacct cactcattag gcaccccagg ctttacactt tatgcttccg gctcctatgt
     9001 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     9061 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
     9121 gccgcgccac ctgggtcctt ttcatcacgt gctataaaaa taattataat ttaaattttt
     9181 taatataaat atataaatta aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt
     9241 tttgttttcc gaagatgtaa aagactctag ggggatcgcc aacaaatact accttttatc
     9301 ttgctcttcc tgctctcagg tattaatgcc gaattgtttc atcttgtctg tgtagaagac
     9361 cacacacgaa aatcctgtga ttttacattt tacttatcgt taatcgaatg tatatctatt
     9421 taatctgctt ttcttgtcta ataaatatat atgtaaagta cgctttttgt tgaaattttt
     9481 taaacctttg tttatttttt tttcttcatt ccgtaactct tctaccttct ttatttactt
     9541 tctaaaatcc aaatacaaaa cataaaaata aataaacaca gagtaaattc ccaaattatt
     9601 ccatcattaa aagatacgag gcgcgtgtaa gttacaggca agcgatccgt cctaaga
//
