LOCUS       Exported               10199 bp ds-DNA     circular SYN 04-MAR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 4, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10199
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     21..37
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(71..87)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..138)
                     /label=Recomb OH 1
     promoter        complement(113..131)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(141..157)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      302..757
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     624..643
                     /label=oRH4736
     promoter        811..1050
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1051..5229
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(5797..5816)
                     /label=oRH4872
     primer_bind     5819..5866
                     /label=oRH5049
     promoter        5847..6080
                     /label=MET17 Promoter
     CDS             6081..7415
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFKLSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     primer_bind     complement(7413..7463)
                     /label=oRH5050
     promoter        7466..7570
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7571..8431
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8602..9190
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        9514..9544
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9552..9568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9576..9592
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9613..9631
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     9660..9687
                     /label=oRH4866
     misc_feature    9686..10189
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     complement(join(10180..10199,1..20))
                     /label=oRH4867
     primer_bind     join(10191..10199,1..21)
                     /label=Recomb OH 2
ORIGIN
        1 agcgctaaga gggcggccgc tctagaacta gtggatcccc cgggctgcag gaattcgata
       61 tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccaattc gccctatagt
      121 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      181 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      241 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      301 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      361 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      421 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      481 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      541 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      601 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      661 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      721 aacgcgaatt ttaacaaaat attaacgttt acaatttcct gatgcggtat tttctcctta
      781 cgcatctgtg cggtatttca caccgcatat aattccactt gcaattacat aaaaaattcc
      841 ggcggttttt cgcgtgtgac tcaatgtcga aatacctgcc taatgaacat gaacatcgcc
      901 caaatgtatt tgaagacccg ctgggagaag ttcaagatat ataagtaaca agcagccaat
      961 agtataaaaa aaaatctgag tttattacct ttcctggaat ttcagtgaaa aactgctaat
     1021 tatagagaga tatcacagag ttactcacta atgactaacg aaaaggtctg gatagagaag
     1081 ttggataatc caactctttc agtgttacca catgactttt tacgcccaca acaagaacct
     1141 tatacgaaac aagctacata ttcgttacag ctacctcagc tcgatgtgcc tcatgatagt
     1201 ttttctaaca aatacgctgt cgctttgagt gtatgggctg cattgatata tagagtaacc
     1261 ggtgacgatg atattgttct ttatattgcg aataacaaaa tcttaagatt caatattcaa
     1321 ccaacgtggt catttaatga gctgtattct acaattaaca atgagttgaa caagctcaat
     1381 tctattgagg ccaatttttc ctttgacgag ctagctgaaa aaattcaaag ttgccaagat
     1441 ctggaaagga cccctcagtt gttccgtttg gcctttttgg aaaaccaaga tttcaaatta
     1501 gacgagttca agcatcattt agtggacttt gctttgaatt tggataccag taataatgcg
     1561 catgttttga acttaattta taacagctta ctgtattcga atgaaagagt aaccattgtt
     1621 gcggaccaat ttactcaata tttgactgct gcgctaagcg atccatccaa ttgcataact
     1681 aaaatctctc tgatcaccgc atcatccaag gatagtttac ctgatccaac taagaacttg
     1741 ggctggtgcg atttcgtggg gtgtattcac gacattttcc aggacaatgc tgaagccttc
     1801 ccagagagaa cctgtgttgt ggagactcca acactaaatt ccgacaagtc ccgttctttc
     1861 acttatcgcg acatcaaccg cacttctaac atagttgccc attatttgat taaaacaggt
     1921 atcaaaagag gtgatgtagt gatgatctat tcttctaggg gtgtggattt gatggtatgt
     1981 gtgatgggtg tcttgaaagc cggcgcaacc ttttcagtta tcgaccctgc atatccccca
     2041 gccagacaaa ccatttactt aggtgttgct aaaccacgtg ggttgattgt tattagagct
     2101 gctggacaat tggatcaact agtagaagat tacatcaatg atgaattgga gattgtttca
     2161 agaatcaatt ccatcgctat tcaagaaaat ggtaccattg aaggtggcaa attggacaat
     2221 ggcgaggatg ttttggctcc atatgatcac tacaaagaca ccagaacagg tgttgtagtt
     2281 ggaccagatt ccaacccaac cctatctttc acatctggtt ccgaaggtat tcctaagggt
     2341 gttcttggta gacatttttc cttggcttat tatttcaatt ggatgtccaa aaggttcaac
     2401 ttaacagaaa atgataaatt cacaatgctg agcggtattg cacatgatcc aattcaaaga
     2461 gatatgttta caccattatt tttaggtgcc caattgtatg tccctactca agatgatatt
     2521 ggtacaccgg gccgtttagc ggaatggatg agtaagtatg gttgcacagt tacccattta
     2581 acacctgcca tgggtcaatt acttactgcc caagctacta caccattccc taagttacat
     2641 catgcgttct ttgtgggtga cattttaaca aaacgtgatt gtctgaggtt acaaaccttg
     2701 gcagaaaatt gccgtattgt taatatgtac ggtaccactg aaacacagcg tgcagtttct
     2761 tatttcgaag ttaaatcaaa aaatgacgat ccaaactttt tgaaaaaatt gaaagatgtc
     2821 atgcctgctg gtaaaggtat gttgaacgtt cagctactag ttgttaacag gaacgatcgt
     2881 actcaaatat gtggtattgg cgaaataggt gagatttatg ttcgtgcagg tggtttggcc
     2941 gaaggttata gaggattacc agaattgaat aaagaaaaat ttgtgaacaa ctggtttgtt
     3001 gaaaaagatc actggaatta tttggataag gataatggtg aaccttggag acaattctgg
     3061 ttaggtccaa gagatagatt gtacagaacg ggtgatttag gtcgttatct accaaacggt
     3121 gactgtgaat gttgcggtag ggctgatgat caagttaaaa ttcgtgggtt cagaatcgaa
     3181 ttaggagaaa tagatacgca catttcccaa catccattgg taagagaaaa cattacttta
     3241 gttcgcaaaa atgccgacaa tgagccaaca ttgatcacat ttatggtccc aagatttgac
     3301 aagccagatg acttgtctaa gttccaaagt gatgttccaa aggaggttga aactgaccct
     3361 atagttaagg gcttaatcgg ttaccatctt ttatccaagg acatcaggac tttcttaaag
     3421 aaaagattgg ctagctatgc tatgccttcc ttgattgtgg ttatggataa actaccattg
     3481 aatccaaatg gtaaagttga taagcctaaa cttcaattcc caactcccaa gcaattaaat
     3541 ttggtagctg aaaatacagt ttctgaaact gacgactctc agtttaccaa tgttgagcgc
     3601 gaggttagag acttatggtt aagtatatta cctaccaagc cagcatctgt atcaccagat
     3661 gattcgtttt tcgatttagg tggtcattct atcttggcta ccaaaatgat ttttacctta
     3721 aagaaaaagc tgcaagttga tttaccattg ggcacaattt tcaagtatcc aacgataaag
     3781 gcctttgccg cggaaattga cagaattaaa tcatcgggtg gatcatctca aggtgaggtc
     3841 gtcgaaaatg tcactgcaaa ttatgcggaa gacgccaaga aattggttga gacgctacca
     3901 agttcgtacc cctctcgaga atattttgtt gaacctaata gtgccgaagg aaaaacaaca
     3961 attaatgtgt ttgttaccgg tgtcacagga tttctgggct cctacatcct tgcagatttg
     4021 ttaggacgtt ctccaaagaa ctacagtttc aaagtgtttg cccacgtcag ggccaaggat
     4081 gaagaagctg catttgcaag attacaaaag gcaggtatca cctatggtac ttggaacgaa
     4141 aaatttgcct caaatattaa agttgtatta ggcgatttat ctaaaagcca atttggtctt
     4201 tcagatgaga agtggatgga tttggcaaac acagttgata taattatcca taatggtgcg
     4261 ttagttcact gggtttatcc atatgccaaa ttgagggatc caaatgttat ttcaactatc
     4321 aatgttatga gcttagccgc cgtcggcaag ccaaagttct ttgactttgt ttcctccact
     4381 tctactcttg acactgaata ctactttaat ttgtcagata aacttgttag cgaagggaag
     4441 ccaggcattt tagaatcaga cgatttaatg aactctgcaa gcgggctcac tggtggatat
     4501 ggtcagtcca aatgggctgc tgagtacatc attagacgtg caggtgaaag gggcctacgt
     4561 gggtgtattg tcagaccagg ttacgtaaca ggtgcctctg ccaatggttc ttcaaacaca
     4621 gatgatttct tattgagatt tttgaaaggt tcagtccaat taggtaagat tccagatatc
     4681 gaaaattccg tgaatatggt tccagtagat catgttgctc gtgttgttgt tgctacgtct
     4741 ttgaatcctc ccaaagaaaa tgaattggcc gttgctcaag taacgggtca cccaagaata
     4801 ttattcaaag actacttgta tactttacac gattatggtt acgatgtcga aatcgaaagc
     4861 tattctaaat ggaagaaatc attggaggcg tctgttattg acaggaatga agaaaatgcg
     4921 ttgtatcctt tgctacacat ggtcttagac aacttacctg aaagtaccaa agctccggaa
     4981 ctagacgata ggaacgccgt ggcatcttta aagaaagaca ccgcatggac aggtgttgat
     5041 tggtctaatg gaataggtgt tactccagaa gaggttggta tatatattgc atttttaaac
     5101 aaggttggat ttttacctcc accaactcat aatgacaaac ttccactgcc aagtatagaa
     5161 ctaactcaag cgcaaataag tctagttgct tcaggtgctg gtgctcgtgg aagctccgca
     5221 gcagcttaag gttgagcatt acgtatgata tgtccatgta caataattaa atatgaatta
     5281 ggagaaagac ttagcttctt ttcgggtgat gtcacttaaa aactccgaga ataatatata
     5341 ataagagaat aaaatattag ttattgaata agaactgtaa atcagctggc gttagtctgc
     5401 taatggcagc ttcatcttgg tttattgtag catgaatcat atttgccttt ttttcctgta
     5461 attcaatgat tcttgcttct atactatcct caatgcaaaa ccttgtgatc ttcacaggtc
     5521 gatactgacc aattctatga actctatcac cactttgcca ttcaacacta gggttccacc
     5581 atgggtctaa aatgaatact tgcgaagcta tatggtgcac tctcagtaca atctgctctg
     5641 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5701 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5761 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5821 tatttttata ggttaatgtc atgatagcca tcctcatgaa aactgtgtaa cataataacc
     5881 gaagtgtcga aaaggtggca ccttgtccaa ttgaacacgc tcgatgaaaa aaataagata
     5941 tatataaggt taagtaaagc gtctgttaga aaggaagttt ttcctttttc ttgctctctt
     6001 gtcttttcat ctactatttc cttcgtgtaa tacagggtcg tcagatacat agatacaatt
     6061 ctattacccc catccataca atgccatctc atttcgatac tgttcaacta cacgccggcc
     6121 aagagaaccc tggtgacaat gctcacagat ccagagctgt accaatttac gccaccactt
     6181 cttatgtttt cgaaaactct aagcatggtt cgcaattgtt tggtctagaa gttccaggtt
     6241 acgtctattc ccgtttccaa aacccaacca gtaatgtttt ggaagaaaga attgctgctt
     6301 tagaaggtgg tgctgctgct ttggctgttt cctccggtca agccgctcaa acccttgcca
     6361 tccaaggttt ggcacacact ggtgacaaca tcgtttccac ttcttactta tacggtggta
     6421 cttataacca gttcaaaatc tcgttcaaaa gatttggtat cgaggctaga tttgttgaag
     6481 gtgacaatcc agaagaattc gaaaaggtct ttgatgaaag aaccaaggct gtttatttgg
     6541 aaaccattgg taatccaaag tacaatgttc cggattttga aaaaattgtt gcaattgctc
     6601 acaaacacgg tattccagtt gtcgttgaca acacatttgg tgccggtggt tacttctgtc
     6661 agccaattaa atacggtgct gatattgtaa cacattctgc taccaaatgg attggtggtc
     6721 atggtactac tatcggtggt attattgttg actctggtaa gttcccatgg aaggactacc
     6781 cagaaaagtt ccctcaattc tctcaacctg ccgaaggata tcacggtact atctacaatg
     6841 aagcctacgg taacttggca tacatcgttc atgttagaac tgaactatta agagatttgg
     6901 gtccattgat gaacccattt gcctctttct tgctactaca aggtgttgaa acattatctt
     6961 tgagagctga aagacacggt gaaaatgcat tgaagttagc caaatggtta gaacaatccc
     7021 catacgtatc ttgggtttca taccctggtt tagcatctca ttctcatcat gaaaatgcta
     7081 agaagtatct atctaacggt ttcggtggtg tcttatcttt cggtgtaaaa gacttaccaa
     7141 atgccgacaa ggaaactgac ccattcaaac tttctggtgc tcaagttgtt gacaatttaa
     7201 agcttgcctc taacttggcc aatgttggtg atgccaagac cttagtcatt gctccatact
     7261 tcactaccca caaacaatta aatgacaaag aaaagttggc atctggtgtt accaaggact
     7321 taattcgtgt ctctgttggt atcgaattta ttgatgacat tattgcagac ttccagcaat
     7381 cttttgaaac tgttttcgct ggccaaaaac catgagtgtg cgtaatgagt tgtagacgtc
     7441 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7501 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7561 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7621 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7681 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7741 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7801 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7861 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7921 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7981 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8041 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8101 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8161 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8221 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8281 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8341 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8401 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8461 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8521 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8581 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8641 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8701 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     8761 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8821 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8881 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8941 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9001 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9061 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9121 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9181 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9241 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9301 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9361 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9421 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9481 tgtgagttac ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcctat
     9541 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     9601 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctcc accgcggtgg
     9661 cggccgcgcc acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt
     9721 tttaatataa atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag
     9781 tttttgtttt ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta
     9841 tcttgctctt cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag
     9901 accacacacg aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta
     9961 tttaatctgc ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt
    10021 tttaaacctt tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac
    10081 tttctaaaat ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta
    10141 ttccatcatt aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcctaaga
//
