LOCUS       Exported               10258 bp ds-DNA     circular SYN 03-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 4, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10258
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     21..37
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(71..87)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..138)
                     /label=Recomb OH 1
     promoter        complement(113..131)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(141..157)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      302..757
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        811..1050
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1051..5229
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     5819..5865
                     /label=oRH5056
     gene            complement(5877..7452)
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     terminator      5877..6074
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(6080..7108)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     promoter        complement(7109..7452)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(7473..7522)
                     /label=oRH5055
     promoter        7525..7629
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7630..8490
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8661..9249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        9573..9603
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9611..9627
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9635..9651
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9672..9690
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     9719..9746
                     /label=oRH4866
     misc_feature    9745..10248
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     complement(join(10239..10258,1..20))
                     /label=oRH4867
     primer_bind     join(10250..10258,1..21)
                     /label=Recomb OH 2
ORIGIN
        1 agcgctaaga gggcggccgc tctagaacta gtggatcccc cgggctgcag gaattcgata
       61 tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccaattc gccctatagt
      121 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      181 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      241 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      301 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      361 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      421 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      481 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      541 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      601 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      661 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      721 aacgcgaatt ttaacaaaat attaacgttt acaatttcct gatgcggtat tttctcctta
      781 cgcatctgtg cggtatttca caccgcatat aattccactt gcaattacat aaaaaattcc
      841 ggcggttttt cgcgtgtgac tcaatgtcga aatacctgcc taatgaacat gaacatcgcc
      901 caaatgtatt tgaagacccg ctgggagaag ttcaagatat ataagtaaca agcagccaat
      961 agtataaaaa aaaatctgag tttattacct ttcctggaat ttcagtgaaa aactgctaat
     1021 tatagagaga tatcacagag ttactcacta atgactaacg aaaaggtctg gatagagaag
     1081 ttggataatc caactctttc agtgttacca catgactttt tacgcccaca acaagaacct
     1141 tatacgaaac aagctacata ttcgttacag ctacctcagc tcgatgtgcc tcatgatagt
     1201 ttttctaaca aatacgctgt cgctttgagt gtatgggctg cattgatata tagagtaacc
     1261 ggtgacgatg atattgttct ttatattgcg aataacaaaa tcttaagatt caatattcaa
     1321 ccaacgtggt catttaatga gctgtattct acaattaaca atgagttgaa caagctcaat
     1381 tctattgagg ccaatttttc ctttgacgag ctagctgaaa aaattcaaag ttgccaagat
     1441 ctggaaagga cccctcagtt gttccgtttg gcctttttgg aaaaccaaga tttcaaatta
     1501 gacgagttca agcatcattt agtggacttt gctttgaatt tggataccag taataatgcg
     1561 catgttttga acttaattta taacagctta ctgtattcga atgaaagagt aaccattgtt
     1621 gcggaccaat ttactcaata tttgactgct gcgctaagcg atccatccaa ttgcataact
     1681 aaaatctctc tgatcaccgc atcatccaag gatagtttac ctgatccaac taagaacttg
     1741 ggctggtgcg atttcgtggg gtgtattcac gacattttcc aggacaatgc tgaagccttc
     1801 ccagagagaa cctgtgttgt ggagactcca acactaaatt ccgacaagtc ccgttctttc
     1861 acttatcgcg acatcaaccg cacttctaac atagttgccc attatttgat taaaacaggt
     1921 atcaaaagag gtgatgtagt gatgatctat tcttctaggg gtgtggattt gatggtatgt
     1981 gtgatgggtg tcttgaaagc cggcgcaacc ttttcagtta tcgaccctgc atatccccca
     2041 gccagacaaa ccatttactt aggtgttgct aaaccacgtg ggttgattgt tattagagct
     2101 gctggacaat tggatcaact agtagaagat tacatcaatg atgaattgga gattgtttca
     2161 agaatcaatt ccatcgctat tcaagaaaat ggtaccattg aaggtggcaa attggacaat
     2221 ggcgaggatg ttttggctcc atatgatcac tacaaagaca ccagaacagg tgttgtagtt
     2281 ggaccagatt ccaacccaac cctatctttc acatctggtt ccgaaggtat tcctaagggt
     2341 gttcttggta gacatttttc cttggcttat tatttcaatt ggatgtccaa aaggttcaac
     2401 ttaacagaaa atgataaatt cacaatgctg agcggtattg cacatgatcc aattcaaaga
     2461 gatatgttta caccattatt tttaggtgcc caattgtatg tccctactca agatgatatt
     2521 ggtacaccgg gccgtttagc ggaatggatg agtaagtatg gttgcacagt tacccattta
     2581 acacctgcca tgggtcaatt acttactgcc caagctacta caccattccc taagttacat
     2641 catgcgttct ttgtgggtga cattttaaca aaacgtgatt gtctgaggtt acaaaccttg
     2701 gcagaaaatt gccgtattgt taatatgtac ggtaccactg aaacacagcg tgcagtttct
     2761 tatttcgaag ttaaatcaaa aaatgacgat ccaaactttt tgaaaaaatt gaaagatgtc
     2821 atgcctgctg gtaaaggtat gttgaacgtt cagctactag ttgttaacag gaacgatcgt
     2881 actcaaatat gtggtattgg cgaaataggt gagatttatg ttcgtgcagg tggtttggcc
     2941 gaaggttata gaggattacc agaattgaat aaagaaaaat ttgtgaacaa ctggtttgtt
     3001 gaaaaagatc actggaatta tttggataag gataatggtg aaccttggag acaattctgg
     3061 ttaggtccaa gagatagatt gtacagaacg ggtgatttag gtcgttatct accaaacggt
     3121 gactgtgaat gttgcggtag ggctgatgat caagttaaaa ttcgtgggtt cagaatcgaa
     3181 ttaggagaaa tagatacgca catttcccaa catccattgg taagagaaaa cattacttta
     3241 gttcgcaaaa atgccgacaa tgagccaaca ttgatcacat ttatggtccc aagatttgac
     3301 aagccagatg acttgtctaa gttccaaagt gatgttccaa aggaggttga aactgaccct
     3361 atagttaagg gcttaatcgg ttaccatctt ttatccaagg acatcaggac tttcttaaag
     3421 aaaagattgg ctagctatgc tatgccttcc ttgattgtgg ttatggataa actaccattg
     3481 aatccaaatg gtaaagttga taagcctaaa cttcaattcc caactcccaa gcaattaaat
     3541 ttggtagctg aaaatacagt ttctgaaact gacgactctc agtttaccaa tgttgagcgc
     3601 gaggttagag acttatggtt aagtatatta cctaccaagc cagcatctgt atcaccagat
     3661 gattcgtttt tcgatttagg tggtcattct atcttggcta ccaaaatgat ttttacctta
     3721 aagaaaaagc tgcaagttga tttaccattg ggcacaattt tcaagtatcc aacgataaag
     3781 gcctttgccg cggaaattga cagaattaaa tcatcgggtg gatcatctca aggtgaggtc
     3841 gtcgaaaatg tcactgcaaa ttatgcggaa gacgccaaga aattggttga gacgctacca
     3901 agttcgtacc cctctcgaga atattttgtt gaacctaata gtgccgaagg aaaaacaaca
     3961 attaatgtgt ttgttaccgg tgtcacagga tttctgggct cctacatcct tgcagatttg
     4021 ttaggacgtt ctccaaagaa ctacagtttc aaagtgtttg cccacgtcag ggccaaggat
     4081 gaagaagctg catttgcaag attacaaaag gcaggtatca cctatggtac ttggaacgaa
     4141 aaatttgcct caaatattaa agttgtatta ggcgatttat ctaaaagcca atttggtctt
     4201 tcagatgaga agtggatgga tttggcaaac acagttgata taattatcca taatggtgcg
     4261 ttagttcact gggtttatcc atatgccaaa ttgagggatc caaatgttat ttcaactatc
     4321 aatgttatga gcttagccgc cgtcggcaag ccaaagttct ttgactttgt ttcctccact
     4381 tctactcttg acactgaata ctactttaat ttgtcagata aacttgttag cgaagggaag
     4441 ccaggcattt tagaatcaga cgatttaatg aactctgcaa gcgggctcac tggtggatat
     4501 ggtcagtcca aatgggctgc tgagtacatc attagacgtg caggtgaaag gggcctacgt
     4561 gggtgtattg tcagaccagg ttacgtaaca ggtgcctctg ccaatggttc ttcaaacaca
     4621 gatgatttct tattgagatt tttgaaaggt tcagtccaat taggtaagat tccagatatc
     4681 gaaaattccg tgaatatggt tccagtagat catgttgctc gtgttgttgt tgctacgtct
     4741 ttgaatcctc ccaaagaaaa tgaattggcc gttgctcaag taacgggtca cccaagaata
     4801 ttattcaaag actacttgta tactttacac gattatggtt acgatgtcga aatcgaaagc
     4861 tattctaaat ggaagaaatc attggaggcg tctgttattg acaggaatga agaaaatgcg
     4921 ttgtatcctt tgctacacat ggtcttagac aacttacctg aaagtaccaa agctccggaa
     4981 ctagacgata ggaacgccgt ggcatcttta aagaaagaca ccgcatggac aggtgttgat
     5041 tggtctaatg gaataggtgt tactccagaa gaggttggta tatatattgc atttttaaac
     5101 aaggttggat ttttacctcc accaactcat aatgacaaac ttccactgcc aagtatagaa
     5161 ctaactcaag cgcaaataag tctagttgct tcaggtgctg gtgctcgtgg aagctccgca
     5221 gcagcttaag gttgagcatt acgtatgata tgtccatgta caataattaa atatgaatta
     5281 ggagaaagac ttagcttctt ttcgggtgat gtcacttaaa aactccgaga ataatatata
     5341 ataagagaat aaaatattag ttattgaata agaactgtaa atcagctggc gttagtctgc
     5401 taatggcagc ttcatcttgg tttattgtag catgaatcat atttgccttt ttttcctgta
     5461 attcaatgat tcttgcttct atactatcct caatgcaaaa ccttgtgatc ttcacaggtc
     5521 gatactgacc aattctatga actctatcac cactttgcca ttcaacacta gggttccacc
     5581 atgggtctaa aatgaatact tgcgaagcta tatggtgcac tctcagtaca atctgctctg
     5641 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5701 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5761 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5821 tatttttata ggttaatgtc atgatctgga tggcggcgtt agtatcgaat cgacagcagt
     5881 atagcgacca gcattcacat acgattgacg catgatatta ctttctgcgc acttaacttc
     5941 gcatctgggc agatgatgtc gaggcgaaaa aaaatataaa tcacgctaac atttgattaa
     6001 aatagaacaa ctacaatata aaaaaactat acaaatgaca agttcttgaa aacaagaatc
     6061 tttttattgt cagtactgat tattcctttg ccctcggacg agtgctgggg cgtcggtttc
     6121 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
     6181 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
     6241 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
     6301 tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga
     6361 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
     6421 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
     6481 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
     6541 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
     6601 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
     6661 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
     6721 ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agaacagcgg
     6781 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat
     6841 aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg agcgcggccg
     6901 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
     6961 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
     7021 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
     7081 tcgcggtgag ttcaggcttt ttacccatgg ttgtttatgt tcggatgtga tgtgagaact
     7141 gtatcctagc aagattttaa aaggaagtat atgaaagaag aacctcagtg gcaaatccta
     7201 accttttata tttctctaca ggggcgcggc gtggggacaa ttcaacgcgt ctgtgagggg
     7261 agcgtttccc tgctcgcagg tctgcagcga ggagccgtaa tttttgcttc gcgccgtgcg
     7321 gccatcaaaa tgtatggatg caaatgatta tacatgggga tgtatgggct aaatgtacgg
     7381 gcgacagtca catcatgccc ctgagctgcg cacgtcaaga ctgtcaagga gggtattctg
     7441 ggcctccatg tcgctggccg ggtgacccgg cggggacgag gcaagctaaa caagacgtca
     7501 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7561 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7621 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7681 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7741 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7801 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7861 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7921 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7981 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8041 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8101 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8161 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8221 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8281 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8341 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8401 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8461 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8521 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8581 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8641 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8701 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8761 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     8821 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8881 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8941 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9001 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9061 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9121 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9181 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9241 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9301 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9361 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9421 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     9481 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     9541 gtgagttacc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcctatg
     9601 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     9661 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc
     9721 ggccgcgcca cctgggtcct tttcatcacg tgctataaaa ataattataa tttaaatttt
     9781 ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt
     9841 ttttgttttc cgaagatgta aaagactcta gggggatcgc caacaaatac taccttttat
     9901 cttgctcttc ctgctctcag gtattaatgc cgaattgttt catcttgtct gtgtagaaga
     9961 ccacacacga aaatcctgtg attttacatt ttacttatcg ttaatcgaat gtatatctat
    10021 ttaatctgct tttcttgtct aataaatata tatgtaaagt acgctttttg ttgaaatttt
    10081 ttaaaccttt gtttattttt ttttcttcat tccgtaactc ttctaccttc tttatttact
    10141 ttctaaaatc caaatacaaa acataaaaat aaataaacac agagtaaatt cccaaattat
    10201 tccatcatta aaagatacga ggcgcgtgta agttacaggc aagcgatccg tcctaaga
//
