LOCUS       Exported               10039 bp ds-DNA     circular SYN 03-APR-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 4, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     21..37
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(71..87)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..138)
                     /label=Recomb OH 1
     promoter        complement(113..131)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(141..157)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      302..757
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        811..1050
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1051..5229
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     5819..5865
                     /label=oRH5056
     gene            complement(5877..7233)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      5877..6074
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(6080..6889)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(6890..7233)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(7254..7303)
                     /label=oRH5055
     promoter        7306..7410
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7411..8271
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8442..9030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        9354..9384
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9392..9408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9416..9432
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9453..9471
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     9500..9527
                     /label=oRH4866
     misc_feature    9526..10029
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     complement(join(10020..10039,1..20))
                     /label=oRH4867
     primer_bind     join(10031..10039,1..21)
                     /label=Recomb OH 2
ORIGIN
        1 agcgctaaga gggcggccgc tctagaacta gtggatcccc cgggctgcag gaattcgata
       61 tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccaattc gccctatagt
      121 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      181 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      241 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      301 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      361 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      421 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      481 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      541 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      601 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      661 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      721 aacgcgaatt ttaacaaaat attaacgttt acaatttcct gatgcggtat tttctcctta
      781 cgcatctgtg cggtatttca caccgcatat aattccactt gcaattacat aaaaaattcc
      841 ggcggttttt cgcgtgtgac tcaatgtcga aatacctgcc taatgaacat gaacatcgcc
      901 caaatgtatt tgaagacccg ctgggagaag ttcaagatat ataagtaaca agcagccaat
      961 agtataaaaa aaaatctgag tttattacct ttcctggaat ttcagtgaaa aactgctaat
     1021 tatagagaga tatcacagag ttactcacta atgactaacg aaaaggtctg gatagagaag
     1081 ttggataatc caactctttc agtgttacca catgactttt tacgcccaca acaagaacct
     1141 tatacgaaac aagctacata ttcgttacag ctacctcagc tcgatgtgcc tcatgatagt
     1201 ttttctaaca aatacgctgt cgctttgagt gtatgggctg cattgatata tagagtaacc
     1261 ggtgacgatg atattgttct ttatattgcg aataacaaaa tcttaagatt caatattcaa
     1321 ccaacgtggt catttaatga gctgtattct acaattaaca atgagttgaa caagctcaat
     1381 tctattgagg ccaatttttc ctttgacgag ctagctgaaa aaattcaaag ttgccaagat
     1441 ctggaaagga cccctcagtt gttccgtttg gcctttttgg aaaaccaaga tttcaaatta
     1501 gacgagttca agcatcattt agtggacttt gctttgaatt tggataccag taataatgcg
     1561 catgttttga acttaattta taacagctta ctgtattcga atgaaagagt aaccattgtt
     1621 gcggaccaat ttactcaata tttgactgct gcgctaagcg atccatccaa ttgcataact
     1681 aaaatctctc tgatcaccgc atcatccaag gatagtttac ctgatccaac taagaacttg
     1741 ggctggtgcg atttcgtggg gtgtattcac gacattttcc aggacaatgc tgaagccttc
     1801 ccagagagaa cctgtgttgt ggagactcca acactaaatt ccgacaagtc ccgttctttc
     1861 acttatcgcg acatcaaccg cacttctaac atagttgccc attatttgat taaaacaggt
     1921 atcaaaagag gtgatgtagt gatgatctat tcttctaggg gtgtggattt gatggtatgt
     1981 gtgatgggtg tcttgaaagc cggcgcaacc ttttcagtta tcgaccctgc atatccccca
     2041 gccagacaaa ccatttactt aggtgttgct aaaccacgtg ggttgattgt tattagagct
     2101 gctggacaat tggatcaact agtagaagat tacatcaatg atgaattgga gattgtttca
     2161 agaatcaatt ccatcgctat tcaagaaaat ggtaccattg aaggtggcaa attggacaat
     2221 ggcgaggatg ttttggctcc atatgatcac tacaaagaca ccagaacagg tgttgtagtt
     2281 ggaccagatt ccaacccaac cctatctttc acatctggtt ccgaaggtat tcctaagggt
     2341 gttcttggta gacatttttc cttggcttat tatttcaatt ggatgtccaa aaggttcaac
     2401 ttaacagaaa atgataaatt cacaatgctg agcggtattg cacatgatcc aattcaaaga
     2461 gatatgttta caccattatt tttaggtgcc caattgtatg tccctactca agatgatatt
     2521 ggtacaccgg gccgtttagc ggaatggatg agtaagtatg gttgcacagt tacccattta
     2581 acacctgcca tgggtcaatt acttactgcc caagctacta caccattccc taagttacat
     2641 catgcgttct ttgtgggtga cattttaaca aaacgtgatt gtctgaggtt acaaaccttg
     2701 gcagaaaatt gccgtattgt taatatgtac ggtaccactg aaacacagcg tgcagtttct
     2761 tatttcgaag ttaaatcaaa aaatgacgat ccaaactttt tgaaaaaatt gaaagatgtc
     2821 atgcctgctg gtaaaggtat gttgaacgtt cagctactag ttgttaacag gaacgatcgt
     2881 actcaaatat gtggtattgg cgaaataggt gagatttatg ttcgtgcagg tggtttggcc
     2941 gaaggttata gaggattacc agaattgaat aaagaaaaat ttgtgaacaa ctggtttgtt
     3001 gaaaaagatc actggaatta tttggataag gataatggtg aaccttggag acaattctgg
     3061 ttaggtccaa gagatagatt gtacagaacg ggtgatttag gtcgttatct accaaacggt
     3121 gactgtgaat gttgcggtag ggctgatgat caagttaaaa ttcgtgggtt cagaatcgaa
     3181 ttaggagaaa tagatacgca catttcccaa catccattgg taagagaaaa cattacttta
     3241 gttcgcaaaa atgccgacaa tgagccaaca ttgatcacat ttatggtccc aagatttgac
     3301 aagccagatg acttgtctaa gttccaaagt gatgttccaa aggaggttga aactgaccct
     3361 atagttaagg gcttaatcgg ttaccatctt ttatccaagg acatcaggac tttcttaaag
     3421 aaaagattgg ctagctatgc tatgccttcc ttgattgtgg ttatggataa actaccattg
     3481 aatccaaatg gtaaagttga taagcctaaa cttcaattcc caactcccaa gcaattaaat
     3541 ttggtagctg aaaatacagt ttctgaaact gacgactctc agtttaccaa tgttgagcgc
     3601 gaggttagag acttatggtt aagtatatta cctaccaagc cagcatctgt atcaccagat
     3661 gattcgtttt tcgatttagg tggtcattct atcttggcta ccaaaatgat ttttacctta
     3721 aagaaaaagc tgcaagttga tttaccattg ggcacaattt tcaagtatcc aacgataaag
     3781 gcctttgccg cggaaattga cagaattaaa tcatcgggtg gatcatctca aggtgaggtc
     3841 gtcgaaaatg tcactgcaaa ttatgcggaa gacgccaaga aattggttga gacgctacca
     3901 agttcgtacc cctctcgaga atattttgtt gaacctaata gtgccgaagg aaaaacaaca
     3961 attaatgtgt ttgttaccgg tgtcacagga tttctgggct cctacatcct tgcagatttg
     4021 ttaggacgtt ctccaaagaa ctacagtttc aaagtgtttg cccacgtcag ggccaaggat
     4081 gaagaagctg catttgcaag attacaaaag gcaggtatca cctatggtac ttggaacgaa
     4141 aaatttgcct caaatattaa agttgtatta ggcgatttat ctaaaagcca atttggtctt
     4201 tcagatgaga agtggatgga tttggcaaac acagttgata taattatcca taatggtgcg
     4261 ttagttcact gggtttatcc atatgccaaa ttgagggatc caaatgttat ttcaactatc
     4321 aatgttatga gcttagccgc cgtcggcaag ccaaagttct ttgactttgt ttcctccact
     4381 tctactcttg acactgaata ctactttaat ttgtcagata aacttgttag cgaagggaag
     4441 ccaggcattt tagaatcaga cgatttaatg aactctgcaa gcgggctcac tggtggatat
     4501 ggtcagtcca aatgggctgc tgagtacatc attagacgtg caggtgaaag gggcctacgt
     4561 gggtgtattg tcagaccagg ttacgtaaca ggtgcctctg ccaatggttc ttcaaacaca
     4621 gatgatttct tattgagatt tttgaaaggt tcagtccaat taggtaagat tccagatatc
     4681 gaaaattccg tgaatatggt tccagtagat catgttgctc gtgttgttgt tgctacgtct
     4741 ttgaatcctc ccaaagaaaa tgaattggcc gttgctcaag taacgggtca cccaagaata
     4801 ttattcaaag actacttgta tactttacac gattatggtt acgatgtcga aatcgaaagc
     4861 tattctaaat ggaagaaatc attggaggcg tctgttattg acaggaatga agaaaatgcg
     4921 ttgtatcctt tgctacacat ggtcttagac aacttacctg aaagtaccaa agctccggaa
     4981 ctagacgata ggaacgccgt ggcatcttta aagaaagaca ccgcatggac aggtgttgat
     5041 tggtctaatg gaataggtgt tactccagaa gaggttggta tatatattgc atttttaaac
     5101 aaggttggat ttttacctcc accaactcat aatgacaaac ttccactgcc aagtatagaa
     5161 ctaactcaag cgcaaataag tctagttgct tcaggtgctg gtgctcgtgg aagctccgca
     5221 gcagcttaag gttgagcatt acgtatgata tgtccatgta caataattaa atatgaatta
     5281 ggagaaagac ttagcttctt ttcgggtgat gtcacttaaa aactccgaga ataatatata
     5341 ataagagaat aaaatattag ttattgaata agaactgtaa atcagctggc gttagtctgc
     5401 taatggcagc ttcatcttgg tttattgtag catgaatcat atttgccttt ttttcctgta
     5461 attcaatgat tcttgcttct atactatcct caatgcaaaa ccttgtgatc ttcacaggtc
     5521 gatactgacc aattctatga actctatcac cactttgcca ttcaacacta gggttccacc
     5581 atgggtctaa aatgaatact tgcgaagcta tatggtgcac tctcagtaca atctgctctg
     5641 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5701 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5761 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5821 tatttttata ggttaatgtc atgatctgga tggcggcgtt agtatcgaat cgacagcagt
     5881 atagcgacca gcattcacat acgattgacg catgatatta ctttctgcgc acttaacttc
     5941 gcatctgggc agatgatgtc gaggcgaaaa aaaatataaa tcacgctaac atttgattaa
     6001 aatagaacaa ctacaatata aaaaaactat acaaatgaca agttcttgaa aacaagaatc
     6061 tttttattgt cagtactgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
     6121 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     6181 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     6241 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     6301 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc
     6361 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     6421 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
     6481 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     6541 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttgccg gggatcgcag
     6601 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     6661 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     6721 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     6781 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     6841 tgttggaatt taatcgcggc ctcgaaacgt gagtcttttc cttacccatg gttgtttatg
     6901 ttcggatgtg atgtgagaac tgtatcctag caagatttta aaaggaagta tatgaaagaa
     6961 gaacctcagt ggcaaatcct aaccttttat atttctctac aggggcgcgg cgtggggaca
     7021 attcaacgcg tctgtgaggg gagcgtttcc ctgctcgcag gtctgcagcg aggagccgta
     7081 atttttgctt cgcgccgtgc ggccatcaaa atgtatggat gcaaatgatt atacatgggg
     7141 atgtatgggc taaatgtacg ggcgacagtc acatcatgcc cctgagctgc gcacgtcaag
     7201 actgtcaagg agggtattct gggcctccat gtcgctggcc gggtgacccg gcggggacga
     7261 ggcaagctaa acaagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     7321 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7381 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     7441 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     7501 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     7561 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     7621 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     7681 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     7741 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     7801 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7861 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     7921 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     7981 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8041 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     8101 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     8161 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     8221 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     8281 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     8341 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     8401 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     8461 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8521 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8581 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8641 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8701 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8761 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8821 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8881 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8941 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9001 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9061 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     9121 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     9181 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     9241 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     9301 agtgagcgca acgcaattaa tgtgagttac ctcactcatt aggcacccca ggctttacac
     9361 tttatgcttc cggctcctat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     9421 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     9481 ctggagctcc accgcggtgg cggccgcgcc acctgggtcc ttttcatcac gtgctataaa
     9541 aataattata atttaaattt tttaatataa atatataaat taaaaataga aagtaaaaaa
     9601 agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct agggggatcg
     9661 ccaacaaata ctacctttta tcttgctctt cctgctctca ggtattaatg ccgaattgtt
     9721 tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat tttacttatc
     9781 gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat atatgtaaag
     9841 tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca ttccgtaact
     9901 cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa taaataaaca
     9961 cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt aagttacagg
    10021 caagcgatcc gtcctaaga
//
