LOCUS       Exported                9154 bp ds-DNA     circular SYN 06-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Hampton lab
  TITLE     Direct Submission
  JOURNAL   Exported Feb 4, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     7..23
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(57..73)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(99..117)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(127..143)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      288..743
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     610..629
                     /label=oRH4736
     promoter        797..1036
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1037..5215
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(5783..5802)
                     /label=oRH4872
     promoter        5854..6069
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             6070..6873
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        6959..7063
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7064..7924
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8095..8683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        9007..9037
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9045..9061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9069..9085
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(9081..9154,1..505)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFA
                     GFPRQALNRGLPLGFRFSALRHLDPKKLD"
     promoter        9106..9124
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(9137..9154,1..90)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
ORIGIN
        1 ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat
       61 accgtcgacc tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc
      121 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      181 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      241 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag
      301 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      361 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      421 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      481 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
      541 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
      601 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
      661 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
      721 caaaatatta acgtttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
      781 atttcacacc gcatataatt ccacttgcaa ttacataaaa aattccggcg gtttttcgcg
      841 tgtgactcaa tgtcgaaata cctgcctaat gaacatgaac atcgcccaaa tgtatttgaa
      901 gacccgctgg gagaagttca agatatataa gtaacaagca gccaatagta taaaaaaaaa
      961 tctgagttta ttacctttcc tggaatttca gtgaaaaact gctaattata gagagatatc
     1021 acagagttac tcactaatga ctaacgaaaa ggtctggata gagaagttgg ataatccaac
     1081 tctttcagtg ttaccacatg actttttacg cccacaacaa gaaccttata cgaaacaagc
     1141 tacatattcg ttacagctac ctcagctcga tgtgcctcat gatagttttt ctaacaaata
     1201 cgctgtcgct ttgagtgtat gggctgcatt gatatataga gtaaccggtg acgatgatat
     1261 tgttctttat attgcgaata acaaaatctt aagattcaat attcaaccaa cgtggtcatt
     1321 taatgagctg tattctacaa ttaacaatga gttgaacaag ctcaattcta ttgaggccaa
     1381 tttttccttt gacgagctag ctgaaaaaat tcaaagttgc caagatctgg aaaggacccc
     1441 tcagttgttc cgtttggcct ttttggaaaa ccaagatttc aaattagacg agttcaagca
     1501 tcatttagtg gactttgctt tgaatttgga taccagtaat aatgcgcatg ttttgaactt
     1561 aatttataac agcttactgt attcgaatga aagagtaacc attgttgcgg accaatttac
     1621 tcaatatttg actgctgcgc taagcgatcc atccaattgc ataactaaaa tctctctgat
     1681 caccgcatca tccaaggata gtttacctga tccaactaag aacttgggct ggtgcgattt
     1741 cgtggggtgt attcacgaca ttttccagga caatgctgaa gccttcccag agagaacctg
     1801 tgttgtggag actccaacac taaattccga caagtcccgt tctttcactt atcgcgacat
     1861 caaccgcact tctaacatag ttgcccatta tttgattaaa acaggtatca aaagaggtga
     1921 tgtagtgatg atctattctt ctaggggtgt ggatttgatg gtatgtgtga tgggtgtctt
     1981 gaaagccggc gcaacctttt cagttatcga ccctgcatat cccccagcca gacaaaccat
     2041 ttacttaggt gttgctaaac cacgtgggtt gattgttatt agagctgctg gacaattgga
     2101 tcaactagta gaagattaca tcaatgatga attggagatt gtttcaagaa tcaattccat
     2161 cgctattcaa gaaaatggta ccattgaagg tggcaaattg gacaatggcg aggatgtttt
     2221 ggctccatat gatcactaca aagacaccag aacaggtgtt gtagttggac cagattccaa
     2281 cccaacccta tctttcacat ctggttccga aggtattcct aagggtgttc ttggtagaca
     2341 tttttccttg gcttattatt tcaattggat gtccaaaagg ttcaacttaa cagaaaatga
     2401 taaattcaca atgctgagcg gtattgcaca tgatccaatt caaagagata tgtttacacc
     2461 attattttta ggtgcccaat tgtatgtccc tactcaagat gatattggta caccgggccg
     2521 tttagcggaa tggatgagta agtatggttg cacagttacc catttaacac ctgccatggg
     2581 tcaattactt actgcccaag ctactacacc attccctaag ttacatcatg cgttctttgt
     2641 gggtgacatt ttaacaaaac gtgattgtct gaggttacaa accttggcag aaaattgccg
     2701 tattgttaat atgtacggta ccactgaaac acagcgtgca gtttcttatt tcgaagttaa
     2761 atcaaaaaat gacgatccaa actttttgaa aaaattgaaa gatgtcatgc ctgctggtaa
     2821 aggtatgttg aacgttcagc tactagttgt taacaggaac gatcgtactc aaatatgtgg
     2881 tattggcgaa ataggtgaga tttatgttcg tgcaggtggt ttggccgaag gttatagagg
     2941 attaccagaa ttgaataaag aaaaatttgt gaacaactgg tttgttgaaa aagatcactg
     3001 gaattatttg gataaggata atggtgaacc ttggagacaa ttctggttag gtccaagaga
     3061 tagattgtac agaacgggtg atttaggtcg ttatctacca aacggtgact gtgaatgttg
     3121 cggtagggct gatgatcaag ttaaaattcg tgggttcaga atcgaattag gagaaataga
     3181 tacgcacatt tcccaacatc cattggtaag agaaaacatt actttagttc gcaaaaatgc
     3241 cgacaatgag ccaacattga tcacatttat ggtcccaaga tttgacaagc cagatgactt
     3301 gtctaagttc caaagtgatg ttccaaagga ggttgaaact gaccctatag ttaagggctt
     3361 aatcggttac catcttttat ccaaggacat caggactttc ttaaagaaaa gattggctag
     3421 ctatgctatg ccttccttga ttgtggttat ggataaacta ccattgaatc caaatggtaa
     3481 agttgataag cctaaacttc aattcccaac tcccaagcaa ttaaatttgg tagctgaaaa
     3541 tacagtttct gaaactgacg actctcagtt taccaatgtt gagcgcgagg ttagagactt
     3601 atggttaagt atattaccta ccaagccagc atctgtatca ccagatgatt cgtttttcga
     3661 tttaggtggt cattctatct tggctaccaa aatgattttt accttaaaga aaaagctgca
     3721 agttgattta ccattgggca caattttcaa gtatccaacg ataaaggcct ttgccgcgga
     3781 aattgacaga attaaatcat cgggtggatc atctcaaggt gaggtcgtcg aaaatgtcac
     3841 tgcaaattat gcggaagacg ccaagaaatt ggttgagacg ctaccaagtt cgtacccctc
     3901 tcgagaatat tttgttgaac ctaatagtgc cgaaggaaaa acaacaatta atgtgtttgt
     3961 taccggtgtc acaggatttc tgggctccta catccttgca gatttgttag gacgttctcc
     4021 aaagaactac agtttcaaag tgtttgccca cgtcagggcc aaggatgaag aagctgcatt
     4081 tgcaagatta caaaaggcag gtatcaccta tggtacttgg aacgaaaaat ttgcctcaaa
     4141 tattaaagtt gtattaggcg atttatctaa aagccaattt ggtctttcag atgagaagtg
     4201 gatggatttg gcaaacacag ttgatataat tatccataat ggtgcgttag ttcactgggt
     4261 ttatccatat gccaaattga gggatccaaa tgttatttca actatcaatg ttatgagctt
     4321 agccgccgtc ggcaagccaa agttctttga ctttgtttcc tccacttcta ctcttgacac
     4381 tgaatactac tttaatttgt cagataaact tgttagcgaa gggaagccag gcattttaga
     4441 atcagacgat ttaatgaact ctgcaagcgg gctcactggt ggatatggtc agtccaaatg
     4501 ggctgctgag tacatcatta gacgtgcagg tgaaaggggc ctacgtgggt gtattgtcag
     4561 accaggttac gtaacaggtg cctctgccaa tggttcttca aacacagatg atttcttatt
     4621 gagatttttg aaaggttcag tccaattagg taagattcca gatatcgaaa attccgtgaa
     4681 tatggttcca gtagatcatg ttgctcgtgt tgttgttgct acgtctttga atcctcccaa
     4741 agaaaatgaa ttggccgttg ctcaagtaac gggtcaccca agaatattat tcaaagacta
     4801 cttgtatact ttacacgatt atggttacga tgtcgaaatc gaaagctatt ctaaatggaa
     4861 gaaatcattg gaggcgtctg ttattgacag gaatgaagaa aatgcgttgt atcctttgct
     4921 acacatggtc ttagacaact tacctgaaag taccaaagct ccggaactag acgataggaa
     4981 cgccgtggca tctttaaaga aagacaccgc atggacaggt gttgattggt ctaatggaat
     5041 aggtgttact ccagaagagg ttggtatata tattgcattt ttaaacaagg ttggattttt
     5101 acctccacca actcataatg acaaacttcc actgccaagt atagaactaa ctcaagcgca
     5161 aataagtcta gttgcttcag gtgctggtgc tcgtggaagc tccgcagcag cttaaggttg
     5221 agcattacgt atgatatgtc catgtacaat aattaaatat gaattaggag aaagacttag
     5281 cttcttttcg ggtgatgtca cttaaaaact ccgagaataa tatataataa gagaataaaa
     5341 tattagttat tgaataagaa ctgtaaatca gctggcgtta gtctgctaat ggcagcttca
     5401 tcttggttta ttgtagcatg aatcatattt gccttttttt cctgtaattc aatgattctt
     5461 gcttctatac tatcctcaat gcaaaacctt gtgatcttca caggtcgata ctgaccaatt
     5521 ctatgaactc tatcaccact ttgccattca acactagggt tccaccatgg gtctaaaatg
     5581 aatacttgcg aagctatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     5641 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     5701 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     5761 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     5821 aatgtcatga tgcaccatac cacagctttt caattcaatt catcattttt tttttattct
     5881 tttttttgat ttcggtttct ttgaaatttt tttgattcgg taatctccga acagaaggaa
     5941 gaacgaagga aggagcacag acttagattg gtatatatac gcatatgtag tgttgaagaa
     6001 acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaacctg caggaaacga
     6061 agataaatca tgtcgaaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt
     6121 gctgccaagc tatttaatat catgcacgaa aagcaaacaa acttgtgtgc ttcattggat
     6181 gttcgtacca ccaaggaatt actggagtta gttgaagcat taggtcccaa aatttgttta
     6241 ctaaaaacac atgtggatat cttgactgat ttttccatgg agggcacagt taagccgcta
     6301 aaggcattat ccgccaagta caatttttta ctcttcgaag acagaaaatt tgctgacatt
     6361 ggtaatacag tcaaattgca gtactctgcg ggtgtataca gaatagcaga atgggcagac
     6421 attacgaatg cacacggtgt ggtgggccca ggtattgtta gcggtttgaa gcaggcggca
     6481 gaagaagtaa caaaggaacc tagaggcctt ttgatgttag cagaattgtc atgcaagggc
     6541 tccctatcta ctggagaata tactaagggt actgttgaca ttgcgaagag cgacaaagat
     6601 tttgttatcg gctttattgc tcaaagagac atgggtggaa gagatgaagg ttacgattgg
     6661 ttgattatga cacccggtgt gggtttagat gacaagggag acgcattggg tcaacagtat
     6721 agaaccgtgg atgatgtggt ctctacagga tctgacatta ttattgttgg aagaggacta
     6781 tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt acagaaaagc aggctgggaa
     6841 gcatatttga gaagatgcgg ccagcaaaac taaaaaactg tattataagt aaatgcatgt
     6901 atactaaact cacaaattag agcttcagac gtcaggtggc acttttcggg gaaatgtgcg
     6961 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7021 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7081 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7141 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7201 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7261 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7321 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7381 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7441 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7501 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7561 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7621 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7681 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7741 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7801 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7861 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7921 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7981 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8041 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8101 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8161 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8221 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     8281 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8341 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8401 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8461 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8521 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8581 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8641 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8701 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8761 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8821 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     8881 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     8941 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tacctcactc attaggcacc
     9001 ccaggcttta cactttatgc ttccggctcc tatgttgtgt ggaattgtga gcggataaca
     9061 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     9121 aagggaacaa aagctggagc tccaccgcgg tggc
//
