LOCUS       Exported                9706 bp ds-DNA     circular SYN 06-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Hampton lab
  TITLE     Direct Submission
  JOURNAL   Exported Feb 4, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     7..23
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(57..73)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(99..117)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(127..143)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      288..743
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        797..1036
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1037..5215
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     gene            complement(5863..7438)
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     terminator      5863..6060
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(6066..7094)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     promoter        complement(7095..7438)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        7511..7615
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7616..8476
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8647..9235
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        9559..9589
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9597..9613
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9621..9637
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(9633..9706,1..505)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFA
                     GFPRQALNRGLPLGFRFSALRHLDPKKLD"
     promoter        9658..9676
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(9689..9706,1..90)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
ORIGIN
        1 ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat
       61 accgtcgacc tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc
      121 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      181 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      241 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag
      301 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      361 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      421 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      481 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
      541 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
      601 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
      661 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
      721 caaaatatta acgtttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
      781 atttcacacc gcatataatt ccacttgcaa ttacataaaa aattccggcg gtttttcgcg
      841 tgtgactcaa tgtcgaaata cctgcctaat gaacatgaac atcgcccaaa tgtatttgaa
      901 gacccgctgg gagaagttca agatatataa gtaacaagca gccaatagta taaaaaaaaa
      961 tctgagttta ttacctttcc tggaatttca gtgaaaaact gctaattata gagagatatc
     1021 acagagttac tcactaatga ctaacgaaaa ggtctggata gagaagttgg ataatccaac
     1081 tctttcagtg ttaccacatg actttttacg cccacaacaa gaaccttata cgaaacaagc
     1141 tacatattcg ttacagctac ctcagctcga tgtgcctcat gatagttttt ctaacaaata
     1201 cgctgtcgct ttgagtgtat gggctgcatt gatatataga gtaaccggtg acgatgatat
     1261 tgttctttat attgcgaata acaaaatctt aagattcaat attcaaccaa cgtggtcatt
     1321 taatgagctg tattctacaa ttaacaatga gttgaacaag ctcaattcta ttgaggccaa
     1381 tttttccttt gacgagctag ctgaaaaaat tcaaagttgc caagatctgg aaaggacccc
     1441 tcagttgttc cgtttggcct ttttggaaaa ccaagatttc aaattagacg agttcaagca
     1501 tcatttagtg gactttgctt tgaatttgga taccagtaat aatgcgcatg ttttgaactt
     1561 aatttataac agcttactgt attcgaatga aagagtaacc attgttgcgg accaatttac
     1621 tcaatatttg actgctgcgc taagcgatcc atccaattgc ataactaaaa tctctctgat
     1681 caccgcatca tccaaggata gtttacctga tccaactaag aacttgggct ggtgcgattt
     1741 cgtggggtgt attcacgaca ttttccagga caatgctgaa gccttcccag agagaacctg
     1801 tgttgtggag actccaacac taaattccga caagtcccgt tctttcactt atcgcgacat
     1861 caaccgcact tctaacatag ttgcccatta tttgattaaa acaggtatca aaagaggtga
     1921 tgtagtgatg atctattctt ctaggggtgt ggatttgatg gtatgtgtga tgggtgtctt
     1981 gaaagccggc gcaacctttt cagttatcga ccctgcatat cccccagcca gacaaaccat
     2041 ttacttaggt gttgctaaac cacgtgggtt gattgttatt agagctgctg gacaattgga
     2101 tcaactagta gaagattaca tcaatgatga attggagatt gtttcaagaa tcaattccat
     2161 cgctattcaa gaaaatggta ccattgaagg tggcaaattg gacaatggcg aggatgtttt
     2221 ggctccatat gatcactaca aagacaccag aacaggtgtt gtagttggac cagattccaa
     2281 cccaacccta tctttcacat ctggttccga aggtattcct aagggtgttc ttggtagaca
     2341 tttttccttg gcttattatt tcaattggat gtccaaaagg ttcaacttaa cagaaaatga
     2401 taaattcaca atgctgagcg gtattgcaca tgatccaatt caaagagata tgtttacacc
     2461 attattttta ggtgcccaat tgtatgtccc tactcaagat gatattggta caccgggccg
     2521 tttagcggaa tggatgagta agtatggttg cacagttacc catttaacac ctgccatggg
     2581 tcaattactt actgcccaag ctactacacc attccctaag ttacatcatg cgttctttgt
     2641 gggtgacatt ttaacaaaac gtgattgtct gaggttacaa accttggcag aaaattgccg
     2701 tattgttaat atgtacggta ccactgaaac acagcgtgca gtttcttatt tcgaagttaa
     2761 atcaaaaaat gacgatccaa actttttgaa aaaattgaaa gatgtcatgc ctgctggtaa
     2821 aggtatgttg aacgttcagc tactagttgt taacaggaac gatcgtactc aaatatgtgg
     2881 tattggcgaa ataggtgaga tttatgttcg tgcaggtggt ttggccgaag gttatagagg
     2941 attaccagaa ttgaataaag aaaaatttgt gaacaactgg tttgttgaaa aagatcactg
     3001 gaattatttg gataaggata atggtgaacc ttggagacaa ttctggttag gtccaagaga
     3061 tagattgtac agaacgggtg atttaggtcg ttatctacca aacggtgact gtgaatgttg
     3121 cggtagggct gatgatcaag ttaaaattcg tgggttcaga atcgaattag gagaaataga
     3181 tacgcacatt tcccaacatc cattggtaag agaaaacatt actttagttc gcaaaaatgc
     3241 cgacaatgag ccaacattga tcacatttat ggtcccaaga tttgacaagc cagatgactt
     3301 gtctaagttc caaagtgatg ttccaaagga ggttgaaact gaccctatag ttaagggctt
     3361 aatcggttac catcttttat ccaaggacat caggactttc ttaaagaaaa gattggctag
     3421 ctatgctatg ccttccttga ttgtggttat ggataaacta ccattgaatc caaatggtaa
     3481 agttgataag cctaaacttc aattcccaac tcccaagcaa ttaaatttgg tagctgaaaa
     3541 tacagtttct gaaactgacg actctcagtt taccaatgtt gagcgcgagg ttagagactt
     3601 atggttaagt atattaccta ccaagccagc atctgtatca ccagatgatt cgtttttcga
     3661 tttaggtggt cattctatct tggctaccaa aatgattttt accttaaaga aaaagctgca
     3721 agttgattta ccattgggca caattttcaa gtatccaacg ataaaggcct ttgccgcgga
     3781 aattgacaga attaaatcat cgggtggatc atctcaaggt gaggtcgtcg aaaatgtcac
     3841 tgcaaattat gcggaagacg ccaagaaatt ggttgagacg ctaccaagtt cgtacccctc
     3901 tcgagaatat tttgttgaac ctaatagtgc cgaaggaaaa acaacaatta atgtgtttgt
     3961 taccggtgtc acaggatttc tgggctccta catccttgca gatttgttag gacgttctcc
     4021 aaagaactac agtttcaaag tgtttgccca cgtcagggcc aaggatgaag aagctgcatt
     4081 tgcaagatta caaaaggcag gtatcaccta tggtacttgg aacgaaaaat ttgcctcaaa
     4141 tattaaagtt gtattaggcg atttatctaa aagccaattt ggtctttcag atgagaagtg
     4201 gatggatttg gcaaacacag ttgatataat tatccataat ggtgcgttag ttcactgggt
     4261 ttatccatat gccaaattga gggatccaaa tgttatttca actatcaatg ttatgagctt
     4321 agccgccgtc ggcaagccaa agttctttga ctttgtttcc tccacttcta ctcttgacac
     4381 tgaatactac tttaatttgt cagataaact tgttagcgaa gggaagccag gcattttaga
     4441 atcagacgat ttaatgaact ctgcaagcgg gctcactggt ggatatggtc agtccaaatg
     4501 ggctgctgag tacatcatta gacgtgcagg tgaaaggggc ctacgtgggt gtattgtcag
     4561 accaggttac gtaacaggtg cctctgccaa tggttcttca aacacagatg atttcttatt
     4621 gagatttttg aaaggttcag tccaattagg taagattcca gatatcgaaa attccgtgaa
     4681 tatggttcca gtagatcatg ttgctcgtgt tgttgttgct acgtctttga atcctcccaa
     4741 agaaaatgaa ttggccgttg ctcaagtaac gggtcaccca agaatattat tcaaagacta
     4801 cttgtatact ttacacgatt atggttacga tgtcgaaatc gaaagctatt ctaaatggaa
     4861 gaaatcattg gaggcgtctg ttattgacag gaatgaagaa aatgcgttgt atcctttgct
     4921 acacatggtc ttagacaact tacctgaaag taccaaagct ccggaactag acgataggaa
     4981 cgccgtggca tctttaaaga aagacaccgc atggacaggt gttgattggt ctaatggaat
     5041 aggtgttact ccagaagagg ttggtatata tattgcattt ttaaacaagg ttggattttt
     5101 acctccacca actcataatg acaaacttcc actgccaagt atagaactaa ctcaagcgca
     5161 aataagtcta gttgcttcag gtgctggtgc tcgtggaagc tccgcagcag cttaaggttg
     5221 agcattacgt atgatatgtc catgtacaat aattaaatat gaattaggag aaagacttag
     5281 cttcttttcg ggtgatgtca cttaaaaact ccgagaataa tatataataa gagaataaaa
     5341 tattagttat tgaataagaa ctgtaaatca gctggcgtta gtctgctaat ggcagcttca
     5401 tcttggttta ttgtagcatg aatcatattt gccttttttt cctgtaattc aatgattctt
     5461 gcttctatac tatcctcaat gcaaaacctt gtgatcttca caggtcgata ctgaccaatt
     5521 ctatgaactc tatcaccact ttgccattca acactagggt tccaccatgg gtctaaaatg
     5581 aatacttgcg aagctatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     5641 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     5701 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     5761 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     5821 aatgtcatga tctggatggc ggcgttagta tcgaatcgac agcagtatag cgaccagcat
     5881 tcacatacga ttgacgcatg atattacttt ctgcgcactt aacttcgcat ctgggcagat
     5941 gatgtcgagg cgaaaaaaaa tataaatcac gctaacattt gattaaaata gaacaactac
     6001 aatataaaaa aactatacaa atgacaagtt cttgaaaaca agaatctttt tattgtcagt
     6061 actgattatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
     6121 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     6181 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     6241 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     6301 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     6361 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     6421 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     6481 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     6541 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     6601 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     6661 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
     6721 gcagcgatcg catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca
     6781 ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg
     6841 ctgaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga
     6901 taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca
     6961 cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg
     7021 tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca
     7081 ggctttttac ccatggttgt ttatgttcgg atgtgatgtg agaactgtat cctagcaaga
     7141 ttttaaaagg aagtatatga aagaagaacc tcagtggcaa atcctaacct tttatatttc
     7201 tctacagggg cgcggcgtgg ggacaattca acgcgtctgt gaggggagcg tttccctgct
     7261 cgcaggtctg cagcgaggag ccgtaatttt tgcttcgcgc cgtgcggcca tcaaaatgta
     7321 tggatgcaaa tgattataca tggggatgta tgggctaaat gtacgggcga cagtcacatc
     7381 atgcccctga gctgcgcacg tcaagactgt caaggagggt attctgggcc tccatgtcgc
     7441 tggccgggtg acccggcggg gacgaggcaa gctaaacaag acgtcaggtg gcacttttcg
     7501 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7561 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7621 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7681 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7741 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7801 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7861 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7921 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7981 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8041 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8101 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8161 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     8221 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8281 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     8341 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8401 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8461 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8521 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8581 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8641 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8701 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8761 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     8821 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8881 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8941 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9001 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9061 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9121 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9181 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9241 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9301 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9361 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9421 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     9481 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttacctcac
     9541 tcattaggca ccccaggctt tacactttat gcttccggct cctatgttgt gtggaattgt
     9601 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     9661 taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggc
//
