LOCUS       Exported                9253 bp ds-DNA     circular SYN 06-JUN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Hampton lab
  TITLE     Direct Submission
  JOURNAL   Exported Feb 4, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     7..23
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(57..73)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(99..117)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(127..143)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      288..743
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        797..1036
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1037..5215
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     gene            complement(5863..6985)
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     terminator      5863..6060
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(6066..6641)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVISYSAWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFAGERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGERQALYMSMPCP"
     promoter        complement(6642..6985)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        7058..7162
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7163..8023
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8194..8782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        9106..9136
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9144..9160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9168..9184
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(9180..9253,1..505)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFA
                     GFPRQALNRGLPLGFRFSALRHLDPKKLD"
     promoter        9205..9223
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(9236..9253,1..90)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
ORIGIN
        1 ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat
       61 accgtcgacc tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc
      121 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      181 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      241 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag
      301 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      361 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      421 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      481 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
      541 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
      601 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
      661 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
      721 caaaatatta acgtttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
      781 atttcacacc gcatataatt ccacttgcaa ttacataaaa aattccggcg gtttttcgcg
      841 tgtgactcaa tgtcgaaata cctgcctaat gaacatgaac atcgcccaaa tgtatttgaa
      901 gacccgctgg gagaagttca agatatataa gtaacaagca gccaatagta taaaaaaaaa
      961 tctgagttta ttacctttcc tggaatttca gtgaaaaact gctaattata gagagatatc
     1021 acagagttac tcactaatga ctaacgaaaa ggtctggata gagaagttgg ataatccaac
     1081 tctttcagtg ttaccacatg actttttacg cccacaacaa gaaccttata cgaaacaagc
     1141 tacatattcg ttacagctac ctcagctcga tgtgcctcat gatagttttt ctaacaaata
     1201 cgctgtcgct ttgagtgtat gggctgcatt gatatataga gtaaccggtg acgatgatat
     1261 tgttctttat attgcgaata acaaaatctt aagattcaat attcaaccaa cgtggtcatt
     1321 taatgagctg tattctacaa ttaacaatga gttgaacaag ctcaattcta ttgaggccaa
     1381 tttttccttt gacgagctag ctgaaaaaat tcaaagttgc caagatctgg aaaggacccc
     1441 tcagttgttc cgtttggcct ttttggaaaa ccaagatttc aaattagacg agttcaagca
     1501 tcatttagtg gactttgctt tgaatttgga taccagtaat aatgcgcatg ttttgaactt
     1561 aatttataac agcttactgt attcgaatga aagagtaacc attgttgcgg accaatttac
     1621 tcaatatttg actgctgcgc taagcgatcc atccaattgc ataactaaaa tctctctgat
     1681 caccgcatca tccaaggata gtttacctga tccaactaag aacttgggct ggtgcgattt
     1741 cgtggggtgt attcacgaca ttttccagga caatgctgaa gccttcccag agagaacctg
     1801 tgttgtggag actccaacac taaattccga caagtcccgt tctttcactt atcgcgacat
     1861 caaccgcact tctaacatag ttgcccatta tttgattaaa acaggtatca aaagaggtga
     1921 tgtagtgatg atctattctt ctaggggtgt ggatttgatg gtatgtgtga tgggtgtctt
     1981 gaaagccggc gcaacctttt cagttatcga ccctgcatat cccccagcca gacaaaccat
     2041 ttacttaggt gttgctaaac cacgtgggtt gattgttatt agagctgctg gacaattgga
     2101 tcaactagta gaagattaca tcaatgatga attggagatt gtttcaagaa tcaattccat
     2161 cgctattcaa gaaaatggta ccattgaagg tggcaaattg gacaatggcg aggatgtttt
     2221 ggctccatat gatcactaca aagacaccag aacaggtgtt gtagttggac cagattccaa
     2281 cccaacccta tctttcacat ctggttccga aggtattcct aagggtgttc ttggtagaca
     2341 tttttccttg gcttattatt tcaattggat gtccaaaagg ttcaacttaa cagaaaatga
     2401 taaattcaca atgctgagcg gtattgcaca tgatccaatt caaagagata tgtttacacc
     2461 attattttta ggtgcccaat tgtatgtccc tactcaagat gatattggta caccgggccg
     2521 tttagcggaa tggatgagta agtatggttg cacagttacc catttaacac ctgccatggg
     2581 tcaattactt actgcccaag ctactacacc attccctaag ttacatcatg cgttctttgt
     2641 gggtgacatt ttaacaaaac gtgattgtct gaggttacaa accttggcag aaaattgccg
     2701 tattgttaat atgtacggta ccactgaaac acagcgtgca gtttcttatt tcgaagttaa
     2761 atcaaaaaat gacgatccaa actttttgaa aaaattgaaa gatgtcatgc ctgctggtaa
     2821 aggtatgttg aacgttcagc tactagttgt taacaggaac gatcgtactc aaatatgtgg
     2881 tattggcgaa ataggtgaga tttatgttcg tgcaggtggt ttggccgaag gttatagagg
     2941 attaccagaa ttgaataaag aaaaatttgt gaacaactgg tttgttgaaa aagatcactg
     3001 gaattatttg gataaggata atggtgaacc ttggagacaa ttctggttag gtccaagaga
     3061 tagattgtac agaacgggtg atttaggtcg ttatctacca aacggtgact gtgaatgttg
     3121 cggtagggct gatgatcaag ttaaaattcg tgggttcaga atcgaattag gagaaataga
     3181 tacgcacatt tcccaacatc cattggtaag agaaaacatt actttagttc gcaaaaatgc
     3241 cgacaatgag ccaacattga tcacatttat ggtcccaaga tttgacaagc cagatgactt
     3301 gtctaagttc caaagtgatg ttccaaagga ggttgaaact gaccctatag ttaagggctt
     3361 aatcggttac catcttttat ccaaggacat caggactttc ttaaagaaaa gattggctag
     3421 ctatgctatg ccttccttga ttgtggttat ggataaacta ccattgaatc caaatggtaa
     3481 agttgataag cctaaacttc aattcccaac tcccaagcaa ttaaatttgg tagctgaaaa
     3541 tacagtttct gaaactgacg actctcagtt taccaatgtt gagcgcgagg ttagagactt
     3601 atggttaagt atattaccta ccaagccagc atctgtatca ccagatgatt cgtttttcga
     3661 tttaggtggt cattctatct tggctaccaa aatgattttt accttaaaga aaaagctgca
     3721 agttgattta ccattgggca caattttcaa gtatccaacg ataaaggcct ttgccgcgga
     3781 aattgacaga attaaatcat cgggtggatc atctcaaggt gaggtcgtcg aaaatgtcac
     3841 tgcaaattat gcggaagacg ccaagaaatt ggttgagacg ctaccaagtt cgtacccctc
     3901 tcgagaatat tttgttgaac ctaatagtgc cgaaggaaaa acaacaatta atgtgtttgt
     3961 taccggtgtc acaggatttc tgggctccta catccttgca gatttgttag gacgttctcc
     4021 aaagaactac agtttcaaag tgtttgccca cgtcagggcc aaggatgaag aagctgcatt
     4081 tgcaagatta caaaaggcag gtatcaccta tggtacttgg aacgaaaaat ttgcctcaaa
     4141 tattaaagtt gtattaggcg atttatctaa aagccaattt ggtctttcag atgagaagtg
     4201 gatggatttg gcaaacacag ttgatataat tatccataat ggtgcgttag ttcactgggt
     4261 ttatccatat gccaaattga gggatccaaa tgttatttca actatcaatg ttatgagctt
     4321 agccgccgtc ggcaagccaa agttctttga ctttgtttcc tccacttcta ctcttgacac
     4381 tgaatactac tttaatttgt cagataaact tgttagcgaa gggaagccag gcattttaga
     4441 atcagacgat ttaatgaact ctgcaagcgg gctcactggt ggatatggtc agtccaaatg
     4501 ggctgctgag tacatcatta gacgtgcagg tgaaaggggc ctacgtgggt gtattgtcag
     4561 accaggttac gtaacaggtg cctctgccaa tggttcttca aacacagatg atttcttatt
     4621 gagatttttg aaaggttcag tccaattagg taagattcca gatatcgaaa attccgtgaa
     4681 tatggttcca gtagatcatg ttgctcgtgt tgttgttgct acgtctttga atcctcccaa
     4741 agaaaatgaa ttggccgttg ctcaagtaac gggtcaccca agaatattat tcaaagacta
     4801 cttgtatact ttacacgatt atggttacga tgtcgaaatc gaaagctatt ctaaatggaa
     4861 gaaatcattg gaggcgtctg ttattgacag gaatgaagaa aatgcgttgt atcctttgct
     4921 acacatggtc ttagacaact tacctgaaag taccaaagct ccggaactag acgataggaa
     4981 cgccgtggca tctttaaaga aagacaccgc atggacaggt gttgattggt ctaatggaat
     5041 aggtgttact ccagaagagg ttggtatata tattgcattt ttaaacaagg ttggattttt
     5101 acctccacca actcataatg acaaacttcc actgccaagt atagaactaa ctcaagcgca
     5161 aataagtcta gttgcttcag gtgctggtgc tcgtggaagc tccgcagcag cttaaggttg
     5221 agcattacgt atgatatgtc catgtacaat aattaaatat gaattaggag aaagacttag
     5281 cttcttttcg ggtgatgtca cttaaaaact ccgagaataa tatataataa gagaataaaa
     5341 tattagttat tgaataagaa ctgtaaatca gctggcgtta gtctgctaat ggcagcttca
     5401 tcttggttta ttgtagcatg aatcatattt gccttttttt cctgtaattc aatgattctt
     5461 gcttctatac tatcctcaat gcaaaacctt gtgatcttca caggtcgata ctgaccaatt
     5521 ctatgaactc tatcaccact ttgccattca acactagggt tccaccatgg gtctaaaatg
     5581 aatacttgcg aagctatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     5641 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     5701 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     5761 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     5821 aatgtcatga tctggatggc ggcgttagta tcgaatcgac agcagtatag cgaccagcat
     5881 tcacatacga ttgacgcatg atattacttt ctgcgcactt aacttcgcat ctgggcagat
     5941 gatgtcgagg cgaaaaaaaa tataaatcac gctaacattt gattaaaata gaacaactac
     6001 aatataaaaa aactatacaa atgacaagtt cttgaaaaca agaatctttt tattgtcagt
     6061 actgattagg ggcagggcat gctcatgtag agcgcctgcc gctcgccgtc cgaggcggtg
     6121 ccgtcgtaca gggcggtgtc caggccgcag agggtgaacc ccatccgccg gtacgcgtgg
     6181 atcgccggtg cgttgacgtt ggtgacctcc agccagaggt gcccggcgcc ccgctcgccg
     6241 gcgaactccg tcgcgagccc catcaacgcg cgcccgaccc cgtgcccccg gtgctccggg
     6301 gcgacctcga tgtcctcgac ggtcagccgg cggttccacg ccgagtacga gatgaccacg
     6361 aagcccgcca ggtcgccgtc gtccccgtac gcgacgaacg tccgggagtc cgggtcgccg
     6421 tcctccccgt cgtccgattc gtcgtccgat tcgtcgtcgg ggaacacctt ggtcaggggc
     6481 gggtccaccg gcacctcccg cagggtgaag ccgtccccgg tggcggtgac gcggaagacg
     6541 gtgtcggtgg tgaaggaccc atccagtgcc tcgatggcct cggcgtcccc cgggacactg
     6601 gtgcggtacc ggtaagccgt gtcgtcaaga gtggtaccca tggttgttta tgttcggatg
     6661 tgatgtgaga actgtatcct agcaagattt taaaaggaag tatatgaaag aagaacctca
     6721 gtggcaaatc ctaacctttt atatttctct acaggggcgc ggcgtgggga caattcaacg
     6781 cgtctgtgag gggagcgttt ccctgctcgc aggtctgcag cgaggagccg taatttttgc
     6841 ttcgcgccgt gcggccatca aaatgtatgg atgcaaatga ttatacatgg ggatgtatgg
     6901 gctaaatgta cgggcgacag tcacatcatg cccctgagct gcgcacgtca agactgtcaa
     6961 ggagggtatt ctgggcctcc atgtcgctgg ccgggtgacc cggcggggac gaggcaagct
     7021 aaacaagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7081 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7141 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     7201 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7261 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7321 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7381 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     7441 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     7501 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7561 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7621 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7681 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7741 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7801 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7861 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     7921 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     7981 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     8041 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8101 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8161 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8221 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8281 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8341 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8401 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     8461 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     8521 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8581 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8641 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8701 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8761 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8821 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     8881 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     8941 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     9001 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     9061 caacgcaatt aatgtgagtt acctcactca ttaggcaccc caggctttac actttatgct
     9121 tccggctcct atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     9181 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     9241 ccaccgcggt ggc
//
