LOCUS       pSM004                  6784 bp    DNA     circular SYN 15-JAN-2019
DEFINITION  pRS306KpnI-Fig1-XhoI-Tgal7-AvrII-[tetO2]4inPgal1-BglII-5'UTR_YML065
            W_4silmut_RS_MfeI-EcoRI-Ter-NotI 
ACCESSION   pSM004
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|pRS306KpnI-Fig1-XhoI-Tgal7-AvrII-[tetO2]4inPgal1-BglII-5'UT
            R_YML065W_4silmut_RS_MfeI-EcoRI-Ter-NotI 
            
FEATURES             Location/Qualifiers
     CDS             715..1575
                     /gene="AmpR"
                     /product="b-lactamase"
                     /label="AmpR CDS"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="AmpR"
                     /SECDescr="b-lactamase"
     CDS             2866..3510
                     /gene="FIG1"
                     /product="ScFIG1p"
                     /label="FIG1 CDS"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="FIG1"
                     /SECDescr="ScFIG1p"
     misc_feature    3733..4078
                     /gene="Gal1 promoter"
                     /label="Gal1 promoter misc"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Gal1 promoter"
     misc_feature    3745..3763
                     /gene="TetO2(1)"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="TetO2(1)"
     misc_feature    3766..3784
                     /gene="TetO2(2)"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="TetO2(2)"
     misc_feature    3786..3804
                     /gene="TetO2(3)"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="TetO2(3)"
     misc_feature    3852..3870
                     /gene="TetO2(4)"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="TetO2(4)"
     misc_feature    4059..4066
                     /gene="TATA box"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="TATA box"
     misc_feature    4059..4078
                     /gene="TATA+12nt"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="TATA+12nt"
     misc_feature    4085..4184
                     /gene="5'UTR"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="5'UTR"
     CDS             4185..4553
                     /gene="YML065W'"
                     /label="YML065W' CDS"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="YML065W'"
     misc_feature    4236..4247
                     /gene="4silentmutations"
                     /product="gag -> gaa, cag -> caa, gga -> ggt, aat -> aac"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="4silentmutations"
                     /SECDescr="gag -> gaa, cag -> caa, gga -> ggt, aat -> aac"
     misc_feature    4448..4453
                     /gene="RS MfeI added"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="RS MfeI added"
     CDS             complement(5571..6374)
                     /gene="URA3"
                     /product="URA3"
                     /label="URA3 CDS"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="URA3"
                     /SECDescr="URA3"
ORIGIN      
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt ttttcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 ggcagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc caggggggaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc cgagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcggaatt
     2761 aaccctcact aaagggaaca aaagctgggt accatcatag agaaatcata tgaaaaatca
     2821 aatacaactc tgggcttgga ggaagtcatt ataagatccg gttacatggg tgtttgtatt
     2881 gataacattc cctcccaata tagctcttac aataatatga ctacattctc caattcaatt
     2941 tgctatgcaa gaaagaattt aagctcggtt cccttataca gagacttgga aattcaactc
     3001 tcaaatattg catcttccag ctccaaaacc caatcaagcg ttgtcttgaa cattttgaag
     3061 ttagcccaat taacttcggt taatgttata catccatatg tcttgatggc aactgtaatc
     3121 ctaacaatcc taatgtttct atttattctg tatgtaactg tccctaagtt accattcaaa
     3181 ctagcggtta ataaatttct actgttgcta agttcaacta tagttttgac atggggtatt
     3241 ggtgcgatgt ggactcatgt agggataaat gcaagttaca gattggttcc atcatcaagt
     3301 atgaatataa tcactgtcaa gaagggcaag aaggcagcag taatggcttg gtttagcttt
     3361 gcattcctcc ttttagacag tgtggtttta tggttaatat ttttaaggga taggaaaagc
     3421 ttgaaagatg aaattgataa tgttccatgt gcccaaaata ggtacaataa ctactcttcg
     3481 gattcatcta cattgcattc caaagtttag ctcgagggtc agattcatta tctacaaaga
     3541 ctgtaaaaag aaagtggaat attcattcat atcatatttt ttctattaac tgcctggttt
     3601 cttttaaatt ttttattggt tgtcgacttg aacggagtga caatatatat atatatatat
     3661 ttaataatga catcattatc tgtaaatctg attcttaatg ctattctagt tatgtaagag
     3721 tggtccccta ggatattgaa gtaatcccta tcagtgatag agaagtccct atcagtgata
     3781 gagaatccct atcagtgata gagatgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg
     3841 cagatgtgcc ttccctatca gtgatagaga aacaataaag attctacaat actagctttt
     3901 atggttatga agaggaaaaa ttggcagtaa cctggcccca caaaccttca aattaacgaa
     3961 tcaaattaac aaccatagga tgataatgcg attagttttt tagccttatt tctggggtaa
     4021 ttaatcagcg aagcgatgat ttttgatcta ttaacagata tataaatgga aaagctgcag
     4081 atctaactgc ataggcggca aattcagcct aaaagtttcc agaagcagga actcattccc
     4141 tattgattaa tactcattac aaaaaccaca atagagtaga taagatggca aaaacgttga
     4201 aggatttaca gggttgggag ataataacaa ctgatgaaca aggtaacata atcgatggag
     4261 gtcagaagag attacgccga agaggtgcaa aaactgaaca ttacttaaag agaagttctg
     4321 atggaattaa actaggtcgt ggtgatagtg tagtcatgca caacgaagcc gctgggactt
     4381 actccgttta tatgatccag gagttgagac ttaatacatt aaataatgtt gtcgaactct
     4441 gggctctcaa ttgcacctat ttacgatggt ttgaagtcaa tcctttagct cattataggc
     4501 agtttaatcc tgacgctaac attttgaatc gtcctttaaa ttattacaat aaagaattct
     4561 gtacaaatag ggccgcatca tgtaattagt tatgtcacgc ttacattcac gccctccccc
     4621 cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta
     4681 ttttcttata gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt
     4741 tctgtacgga cgcgtgtacg catctagcta gagcggccgc caccgcggtg gagctccaat
     4801 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
     4861 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4921 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4981 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5041 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5101 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5161 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5221 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5281 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5341 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5401 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcctgatgc ggtattttct
     5461 ccttacgcat ctgtgcggta tttcacaccg cagggtaata actgatataa ttaaattgaa
     5521 gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc
     5581 tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc
     5641 ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta
     5701 gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa
     5761 cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt
     5821 tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta
     5881 gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct
     5941 ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc
     6001 accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag
     6061 tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa
     6121 ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc
     6181 aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc
     6241 agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc
     6301 atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat
     6361 gtagctttcg acatgattta tcttcgtttc ctgcaggttt ttgttctgtg cagttgggtt
     6421 aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata taccaatcta
     6481 agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt
     6541 caaagaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa ttgaaaagcg
     6601 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     6661 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     6721 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     6781 gcga
//
