LOCUS       pSV_238                 9086 bp    DNA     circular SYN 15-JAN-2019
DEFINITION  pRS303SalI-Pret2.rTetR.SUM1.Tact1-NotI
ACCESSION   pSV_238
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|pRS303SalI-Pret2.rTetR.SUM1.Tact1-NotI
FEATURES             Location/Qualifiers
     CDS             191..1051
                     /gene="Ampicillin"
                     /product="b-lactamase"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Ampicillin"
                     /SECDescr="b-lactamase"
     CDS             2994..3617
                     /gene="rtTA (rTetR only)"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="rtTA (rTetR only)"
     CDS             3618..6815
                     /gene="SUM1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="SUM1"
     CDS             complement(7925..8584)
                     /gene="HIS3"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="HIS3"
ORIGIN      
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataagacgtc
       61 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      121 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      181 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      241 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      301 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      361 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      421 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      481 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      541 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      601 gacaacgatc ggaggaccga aggagctaac cgcttttttt cacaacatgg gggatcatgt
      661 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      721 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      781 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      841 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      901 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      961 agttatctac acgacgggca gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     1021 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1081 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1141 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1201 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1261 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1321 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     1381 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1441 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1501 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1561 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
     1621 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1681 aacaggagag cgcacgaggg agcttccagg ggggaacgcc tggtatcttt atagtcctgt
     1741 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggccgag
     1801 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1861 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1921 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1981 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2041 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2101 tgtgagttac ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcctat
     2161 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2221 cgccaagctc ggaattaacc ctcactaaag ggaacaaaag ctgggtaccg ggccccccct
     2281 cgaggtcgac attacgatgg cttcttatct cacttcaata gtactttcca ccggttatac
     2341 ttccggcttt tccctattaa tacaagctac aatttcaatg ggtggcaaat aatgtgtaga
     2401 atagaaaata agccgacagg gtaataaaga aaatttttag aaaaaaaagg ttagatggct
     2461 tatttaagtt acaggctagc gaaaaaagga acttcagggc aagtaaagtg tttgattggg
     2521 cactagcatg gcttataaag gcgagcaatt gtcgaaacta attaatgttg tacggactat
     2581 tgctgtcttc tcgtggtaaa tgcgtgttcc aggtcgaata ctacttgcac acaggcgagc
     2641 ggggccccat aaaagtgttg ccgatttgtt aagttgtctt ttcggttttt ctactctgtt
     2701 attccttact tcccttttta agaactcttt ttatccttca tttaggatct tgcacgtttc
     2761 cgcctcatca cttgaattaa aacatgtctc tgtcagtaaa ccttggcgtt tctattgttc
     2821 ttcatagttc aacttttatt attacccgcc ctgcgcgttt acatttttcc agcaacagcc
     2881 agcgaaaaat tagaaaatct ggttgttgac acctcaagaa caagggcaat tagcctcagc
     2941 gtcgaatata gatcatatta gaatacctat agctccatca aaagaaatac aaaatgccta
     3001 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatggg gtcggaatcg
     3061 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt
     3121 ggcatgtaaa aaataagcgg gctttgctcg acgccttacc cattgagatg ttagataggc
     3181 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg
     3241 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta
     3301 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac
     3361 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcac tttactttag
     3421 gttgcgtatt ggaagaacaa gagcatcaag tcgctaaaga agaaagggaa acacctacta
     3481 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcgc caaggtgcag
     3541 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat
     3601 gtgaaagtgg gtccgcgtcc gcgggcgcat ctgagaacac cacagcccct tctgataaca
     3661 taaccaatga acagagactt ccgtctggac ccaaagatga cgtagatacg ttagcactta
     3721 ccagtgctca aaatcaagct aatagtttga ggaagctaga tacagatgcg aacgctaaag
     3781 ctttgccatc catcacagac ataccagtca gtgatgattt tgatataaaa cgacaagttg
     3841 gctctggttt tggttcaaat ccgcttcata taaaagattc cgaggccttt ccacattcat
     3901 ctattgaagc cctaaaagag ggtatggaca aagtcacaaa acaatgcaac gatttgaaga
     3961 cggctttgct ttccaaagac acttccctca ctgattctgt gcaggatttg tttaactcat
     4021 taaaagtttt atcacacaac caatcagttt tggaaaataa gctggatgat gtcatgaaga
     4081 atcaagtaaa tactgacata ttggtcaata atttgaatga acgattgaac aaattatcga
     4141 caatgttgca aaatacttcg aaagtaaatc actctaacct tctcatagaa aattcatcca
     4201 acaataccag ttcacagcat aatacctctt cttctcgaag gggacctggc aggccaagaa
     4261 aagatgcctc gacctccaca atgaataagt tagtgtcaaa cgcagcctcg gtcaatctca
     4321 aaagtgcatc caatcagggt gctcctttct caccagtaaa tattacttta ccgactgccg
     4381 tagtacaaac gtctaaatcc aaaaggtatt ttgtggaacc atcaacgaaa caagagtcgc
     4441 ttttattatc tgccccttca tcatcaagag atgatgctga tatgtccttg acttccgtac
     4501 cgcaaagaac gaacaatgaa aatggtaaag agcgaccatc cactgctaat tctagctcca
     4561 tcacaccaac acctgttacg ccgaacaact taattcaaat caaaagaaaa agaggcaggc
     4621 ccccaaaaaa gagaacagtg gaaacaatga tatccaattc cacggatacg atagataagt
     4681 cagatgcatc taatcggatt aaaaacgaga ttccaataaa ttccttgctt ccatcttcca
     4741 aatttcatca aataccatca tctccatcca atcctgtgtc acaaccggct ccggttcgga
     4801 cttcaaggtc agccacacaa gaaatagaca ttaaaagttt agaactggcc tctctaattt
     4861 caactaatgg tgatccaaac gcagaggatt cgaatactac tgatactgtt cataataacg
     4921 tggaaggaaa ggtaaatgtt gaagaaaata aaaccgagaa ggagaaaata ataaccatca
     4981 aatcatccag cgaaaatagt ggtaataata cgaccaataa taataatact gacaacgtca
     5041 ttaaattttc agctaattca gatatcaata gtgatattcg ccgattaatg gttaacgatc
     5101 agttttcatt aagttatgac gccagcggta atattacggt caaattacca cccgtttcct
     5161 ctccagcagc agcaacagct gccgctgctg cggcggttac gtcagagatg aatagacaac
     5221 aaagagaatt agataaaaga cgtgattcaa gggagaaaat gcttgttaat atgaaatata
     5281 acgatcgcga taaagcaaag tcatttatgg agtctaataa gaaactctta aaagcaatga
     5341 aagaagaaga gaggaggaaa agaatgactt cgataataca tgataatcac ttgaacttaa
     5401 atttgaatga gatctccacc cgttcaaaga taaaaagtgc agaaaaacca acaactaaag
     5461 gttcttcaat gtcgccaaaa ccaagatcgg cctccatcag cggcatttca gaccaccaac
     5521 aggaaggata tcaaccatta gagcaagaaa aactcgtcga tattgacaat gagggctcaa
     5581 acgcaaacag cgactctctc aagatgggtc taaccatatc cgctgccgat acggttcaca
     5641 aagttggaat acagtccatg ctaaattctg gggaagaggc aattaccaag gagaatgcag
     5701 aatatgaaag aaagaccccg ggagatgaag aaactaccac gttcgttcca ttagaaaact
     5761 cacaaccttc ggacacgata aggaaaagaa cagcaggcga tgacggtgca ttggatcaaa
     5821 cagagaatac gagcatatca ccaaagaaga gacgcacaga agatcataca aaaggtgaag
     5881 aagatgaggg agaaaggggt gttggtaata gcggaacgct ggccaccgtg gagaatgtct
     5941 caggagacat ctccgcagat ttatccaaag gtacttcatc tatccataac gatactgagt
     6001 ctgctaacga tagtagcaat ggcaacggaa acctcggttt gggtacagag tcacgcaata
     6061 cattgttaac ggcaactccc attgaattaa tctgccggga aggcttcttc taccggagag
     6121 atattccgga cgttcccatt accacaggag cgtacctgga atttaaattc aaggcgaaag
     6181 aagaggaatt gatcaactct agcatcaatg aggaggatta tgctgctaaa tcaaagcatg
     6241 aaaaaatgaa tgcgcatttc ttcaagcctg atattcaaga agagacggaa cttgcctttg
     6301 aaattttgag caagacaacg ctgacagaga aatacgttaa cagtttggag tacttcttga
     6361 tggaattcag gtgggagaat aaactagttg gtctaggctt aaaacttcgg gaatccaaaa
     6421 gaacctggca aaggagaaag gcgttatttg cccttttcga attttggaga gaccaatcaa
     6481 gagacaaaag aagattccac aactacacca tcctgcatgc ggtgaaagag atggaaaact
     6541 acagaatatt tattaatcga tccgtttcat ggttctacaa ccatattacc ctgctaaaaa
     6601 tgatcctcta cgacctctgc gacaatgtga ccactcaatg gagagagtgg atgtttcccc
     6661 ataacgaaac actgccggca ttgggtcagg acggcattaa cgaagacaat ctgaatgaaa
     6721 ccatcgacaa catgttaatt tttgacttcc ttgatgacgg ttcagaaaac aaccaggtca
     6781 aatattccag aatcataccg ccagatatcc gttaacaatt caaaaatatg gactgcattt
     6841 gaactagtgt ttctaatctc tgcttttgtg cgcgtatgtt tatgtatgta cctctctctc
     6901 tatttctatt tttaaaccac cctctcaata aaataaaaat aataaagtat ttttaaggaa
     6961 aagacgtgtt taagcactga ctttatctac gcggccgcca ccgcggtgga gctccaattc
     7021 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
     7081 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccccttcg ccagctggcg
     7141 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     7201 atggcgcgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     7261 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     7321 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     7381 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     7441 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     7501 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     7561 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7621 aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcctgat gcggtatttt
     7681 ctccttacgc atctgtgcgg tatttcacac cgcatatgat ccgtcgagtt caagagaaaa
     7741 aaaaagaaaa agcaaaaaga aaaaaggaaa gcgcgcctcg ttcagaatga cacgtataga
     7801 atgatgcatt accttgtcat cttcagtatc atactgttcg tatacatact tactgacatt
     7861 cataggtata catatataca catgtatata tatcgtatgc tgcagcttta aataatcggt
     7921 gtcactacat aagaacacct ttggtggagg gaacatcgtt ggtaccattg ggcgaggtgg
     7981 cttctcttat ggcaaccgca agagccttga acgcactctc actacggtga tgatcattct
     8041 tgcctcgcag acaatcaacg tggagggtaa ttctgctagc ctctgcaaag ctttcaagaa
     8101 aatgcgggat catctcgcaa gagagatctc ctactttctc cctttgcaaa ccaagttcga
     8161 caactgcgta cggcctgttc gaaagatcta ccaccgctct ggaaagtgcc tcatccaaag
     8221 gcgcaaatcc tgatccaaac ctttttactc cacgcgccag tagggcctct ttaaaagctt
     8281 gaccgagagc aatcccgcag tcttcagtgg tgtgatggtc gtctatgtgt aagtcaccaa
     8341 tgcactcaac gattagcgac cagccggaat gcttggccag agcatgtatc atatggtcca
     8401 gaaaccctat acctgtgtgg acgttaatca cttgcgattg tgtggcctgt tctgctactg
     8461 cttctgcctc tttttctggg aagatcgagt gctctatcgc taggggacca ccctttaaag
     8521 agatcgcaat ctgaatcttg gtttcatttg taatacgctt tactagggct ttctgctctg
     8581 tcatctttgc cttcgtttat cttgcctgct cattttttag tatattcttc gaagaaatca
     8641 cattacttta tataatgtat aattcattat gtgataatgc caatcgctaa gaaaaaaaaa
     8701 gagtcatccg ctaggtggaa aaaaaaaaat gaaaatcatt accgaggcat aaaaaaatat
     8761 agagtgtact agaggaggcc aagagtaata gaaaaagaaa attgcgggaa aggactgtgt
     8821 tatgacttcc ctgactaatg ccgtgttcaa acgatacctg gcagtgactc ctagcgctca
     8881 ccaagctctt aaaacggaat tatggtgcac tctcagtaca atctgctctg atgccgcata
     8941 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     9001 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     9061 ttcaccgtca tcaccgaaac gcgcga
//
