LOCUS       Exported                7172 bp ds-DNA     circular SYN 20-MAR-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 20, 2019 from SnapGene 4.3.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        160..639
                     /label=hSYN Promoter
                     /note="color: #ffff1f"
     CDS             665..1384
                     /locus_tag="mCherry"
                     /label=mCherry
                     /note="color: #ffc5bb; direction: RIGHT"
     CDS             1999..2343
                     /codon_start=1
                     /label=SspB
                     /note="color: #ffc5bb"
                     /translation="MEYSSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVK
                     DGQIVLNLSASATGNLQLTNDFIQFNAQFKGVSRELYIPMGAALAIYARENGDGVMFEP
                     EEIYDELNIEL"
     misc_feature    2167..2169
                     /label=A58
                     /note="color: #00ffff"
     misc_feature    2215..2217
                     /label=R73
                     /note="color: #00ffff"
     misc_feature    2344..2370
                     /label=linker
                     /note="color: #cccccc"
     misc_feature    2344..2370
                     /label=New Feature
                     /note="color: #00ffff"
     misc_feature    2371..2388
                     /label=linker
                     /label=linker(1)
                     /note="color: #ff1dd5"
     misc_feature    2389..3273
                     /label=BoNT/B 147-441
                     /note="color: #ff8a30"
     misc_feature    2419..2421
                     /label=N156
                     /note="color: #00ffff"
     misc_feature    2452..2454
                     /label=N167
                     /note="color: #00ffff"
     misc_feature    2854..2856
                     /label=R301
                     /note="color: #00ffff"
     misc_feature    2881..2886
                     /label=S310, D311
                     /note="color: #00ffff"
     misc_feature    2929..2931
                     /label=K326
                     /note="color: #00ffff"
     misc_feature    3043..3045
                     /label=Y364
                     /note="color: #00ffff"
     polyA_signal    3918..4398
                     /label=hGH polyA signal
                     /note="color: #ffc0cb"
     rep_origin      4650..5105
                     /label=M13 origin
                     /note="color: #808080"
     rep_origin      4667..4973
                     /label=F1 ori
                     /note="color: #808080"
     CDS             5690..6349
                     /label=AmpR
                     /note="color: #ffff00; direction: RIGHT"
     rep_origin      6447..7129
                     /label=ColE1 origin
                     /note="color: #808080"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat ctgctagcgc
      661 caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
      721 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
      781 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
      841 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
      901 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
      961 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     1021 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     1081 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     1141 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     1201 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     1261 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     1321 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtgtggcgg
     1381 ctagtactcc ggtattgcgg tacccttgta cgcctgtttt atactccctt cccgtaactt
     1441 agacgcacaa aaccaagttc aatagaaggg ggtacaaacc agtaccacca cgaacaagca
     1501 cttctgtttc cccggtgatg tcgtatagac tgcttgcgtg gttgaaagcg acggatccgt
     1561 tatccgctta tgtacttcga gaagcccagt accacctcgg aatcttcgat gcgttgcgct
     1621 cagcactcaa ccccagagtg tagcttaggc tgatgagtct ggacatccct caccggtgac
     1681 ggtggtccag gctgcgttgg cggcctacct atggctaacg ccatgggacg ctagttgtga
     1741 acaaggtgtg aagagcctat tgagctacat aagaatcctc cggcccctga atgcggctaa
     1801 tcccaacctc ggagcaggtg gtcacaaacc agtgattggc ctgtcgtaac gcgcaagtcc
     1861 gtggcggaac cgactacttt gggtgtccgt gtttcctttt attttattgt ggctgcttat
     1921 ggtgacaatc acagattgtt atcataaagc gaattggata ggatcaagct tatcgatacc
     1981 gtcgacctcg aggccaccat ggaatacagc tccccgaaac gccctaagct gctgcgtgaa
     2041 tattacgatt ggctggttga taacagcttt accccatatc tggtggtgga tgccacatac
     2101 ctgggcgtga acgtgcccgt ggagtatgtg aaagacggtc agatcgtgct gaatctgtct
     2161 gcaagtgcga ccggcaacct gcaactgaca aatgatttta tccagttcaa cgcccagttt
     2221 aagggcgtgt ctcgtgaact gtatatcccg atgggtgccg ctctggccat ttacgctcgc
     2281 gagaacggcg atggtgtgat gttcgaacca gaagaaatct atgacgagct gaatattgag
     2341 ctcggtggcg gtggctctgg aggtggtggg tccggaggag gcggccgcga agtggagcga
     2401 aaaaaaggta ttttcgcaaa tttaataata tttggacctg ggccagtttt aaatgaaaat
     2461 gagactatag atataggtat acaaaatcat tttgcatcaa gggaaggctt cgggggtata
     2521 atgcaaatga agttttgccc agaatatgta agcgtattta ataatgttca agaaaacaaa
     2581 ggcgcaagta tatttaatag acgtggatat ttttcagatc cagccttgat attaatgcat
     2641 gaacttatac atgttttaca tggattatat ggcattaaag tagatgattt accaattgta
     2701 ccaaatgaaa aaaaattttt tatgcaatct acagatgcta tacaggcaga agaactatat
     2761 acatttggag gacaagatcc cagcatcata actccttcta cggataaaag tatctatgat
     2821 aaagttttgc aaaattttag agggatagtt gatagactta acaaggtttt agtttgcata
     2881 tcagatccta acattaatat taatatatat aaaaataaat ttaaagataa atataaattc
     2941 gttgaagatt ctgagggaaa atatagtata gatgtagaaa gttttgataa attatataaa
     3001 agcttaatgt ttggttttac agaaactaat atagcagaaa atgccaaaat aaaaactaga
     3061 gcttcttatt ttagtgattc cttaccacca gtaaaaataa aaaatttatt agataatgaa
     3121 atctatacta tagaggaagg gtttaatata tctgataaag atatggaaaa agaatataga
     3181 ggtcagaata aagctataaa taaacaagct tatgaagaaa ttagcaagga gcatttggct
     3241 gtatataaga tacaaatgtg taaaagtgtt aaatgaattc gatatcaagc ttatcgataa
     3301 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3361 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3421 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3481 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3541 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3601 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3661 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     3721 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3781 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3841 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgagc
     3901 gctgctcgag agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
     3961 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
     4021 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
     4081 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt
     4141 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg
     4201 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
     4261 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct
     4321 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct
     4381 cccttccctg tccttctgat tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac
     4441 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     4501 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     4561 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     4621 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     4681 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4741 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4801 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4861 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4921 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4981 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5041 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     5101 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     5161 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     5221 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     5281 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5341 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5401 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5461 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5521 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5581 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5641 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5701 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5761 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5821 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5881 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5941 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6001 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6061 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6121 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6181 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6241 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6301 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6361 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6421 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6481 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6541 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6601 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6661 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6721 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6781 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6841 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6901 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6961 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7021 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7081 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7141 cctggccttt tgctggcctt ttgctcacat gt
//
