LOCUS       pDONR223_LC3A_G120A.xdna    3159 bp      DNA     circular   11/02/2019
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=GateWay(tm) BP reaction between PCR(AttB-LC3B wt) as an Expr
            ession fragment and pDONR223 as a Donor vector.
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     terminator      complement(270..297)
                     /label=rrnB T2 terminator
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="rrnB_T2_terminator"complement(268..295) \ 
     terminator      complement(429..472)
                     /label=rrnB T1 terminator
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="rrnB_T1_terminator" - complement(427..470) \ 
     misc_feature    524..546
                     /label=M13 pUC fwd primer
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_pUC_fwd_primer" 522..544 \ 
     promoter        539..555
                     /label=M13 forward20 primer
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_forward20_primer" 537..553 \ 
     misc_feature    complement(571..654)
                     /label=attL1
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="attL1"complement(569..652) \ 
     misc_feature    1052..1139
                     /label=attL2
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="attL2" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCD \ 
     promoter        complement(1157..1175)
                     /label=T7 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="T7_promoter" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNE \ 
     promoter        complement(1176..1194)
                     /label=M13 reverse primer
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="M13_reverse_primer" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFH \ 
     gene            1408..2418
                     /label=spect
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="spect" - gene="spect" - translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF - LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF - SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV - ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCD \ 
     rep_origin      2495..3114
                     /label=pBR322_origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label="pBR322_origin" - translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA - RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL - AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE - VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLA \ 
     CDS             673..1030
                     /label=LC3A [*]
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             1031..1031
                     /label=LC3A [*] [5']
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             1033..1035
                     /label=LC3A [*] [3']
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT 
       61 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA 
      121 GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG 
      181 CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TACGCGTACC 
      241 GCTAGCCAGG AAGAGTTTGT AGAAACGCAA AAAGGCCATC CGTCAGGATG GCCTTCTGCT 
      301 TAGTTTGATG CCTGGCAGTT TATGGCGGGC GTCCTGCCCG CCACCCTCCG GGCCGTTGCT 
      361 TCACAACGTT CAAATCCGCT CCCGGCGGAT TTGTCCTACT CAGGAGAGCG TTCACCGACA 
      421 AACAACAGAT AAAACGAAAG GCCCAGTCTT CCGACTGAGC CTTTCGTTTT ATTTGATGCC 
      481 TGGCAGTTCC CTACTCTCGC GTTAACGCTA GCATGGATGT TTTCCCAGTC ACGACGTTGT 
      541 AAAACGACGG CCAGTCTTAA GCTCGGGCCC CAAATAATGA TTTTATTTTG ACTGATAGTG 
      601 ACCTGTTCGT TGCAACAAAT TGATGAGCAA TGCTTTTTTA TAATGCCAAC TTTGTACAAA 
      661 AAAGCAGGCT TAATGCCCTC AGACCGGCCT TTCAAGCAGC GGCGGAGCTT CGCCGACCGC 
      721 TGTAAGGAGG TACAGCAGAT CCGCGACCAG CACCCCAGCA AAATCCCGGT GATCATCGAG 
      781 CGCTACAAGG GTGAGAAGCA GCTGCCCGTC CTGGACAAGA CCAAGTTTTT GGTCCCGGAC 
      841 CATGTCAACA TGAGCGAGTT GGTCAAGATC ATCCGGCGCC GCCTGCAGCT GAACCCCACG 
      901 CAGGCCTTCT TCCTGCTGGT GAACCAGCAC AGCATGGTGA GTGTGTCCAC GCCCATCGCG 
      961 GACATCTACG AGCAGGAGAA AGACGAGGAC GGCTTCCTCT ATATGGTCTA CGCCTCCCAG 
     1021 GAAACCTTCG CCTTCTAATA CCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT 
     1081 TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC 
     1141 CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG 
     1201 CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAA TATATCATCA 
     1261 TGCCTCCTCT AGACCAGCCA GGACAGAAAT GCCTCGACTT CGCTGCTGCC CAAGGTTGCC 
     1321 GGGTGACGCA CACCGTGGAA ACGGATGAAG GCACGAACCC AGTGGACATA AGCCTGTTCG 
     1381 GTTCGTAAGC TGTAATGCAA GTAGCGTATG CGCTCACGCA ACTGGTCCAG AACCTTGACC 
     1441 GAACGCAGCG GTGGTAACGG CGCAGTGGCG GTTTTCATGG CTTGTTATGA CTGTTTTTTT 
     1501 GGGGTACAGT CTATGCCTCG GGCATCCAAG CAGCAAGCGC GTTACGCCGT GGGTCGATGT 
     1561 TTGATGTTAT GGAGCAGCAA CGATGTTACG CAGCAGGGCA GTCGCCCTAA AACAAAGTTA 
     1621 AACATCATGA GGGAAGCGGT GATCGCCGAA GTATCGACTC AACTATCAGA GGTAGTTGGC 
     1681 GTCATCGAGC GCCATCTCGA ACCGACGTTG CTGGCCGTAC ATTTGTACGG CTCCGCAGTG 
     1741 GATGGCGGCC TGAAGCCACA CAGTGATATT GATTTGCTGG TTACGGTGAC CGTAAGGCTT 
     1801 GATGAAACAA CGCGGCGAGC TTTGATCAAC GACCTTTTGG AAACTTCGGC TTCCCCTGGA 
     1861 GAGAGCGAGA TTCTCCGCGC TGTAGAAGTC ACCATTGTTG TGCACGACGA CATCATTCCG 
     1921 TGGCGTTATC CAGCTAAGCG CGAACTGCAA TTTGGAGAAT GGCAGCGCAA TGACATTCTT 
     1981 GCAGGTATCT TCGAGCCAGC CACGATCGAC ATTGATCTGG CTATCTTGCT GACAAAAGCA 
     2041 AGAGAACATA GCGTTGCCTT GGTAGGTCCA GCGGCGGAGG AACTCTTTGA TCCGGTTCCT 
     2101 GAACAGGATC TATTTGAGGC GCTAAATGAA ACCTTAACGC TATGGAACTC GCCGCCCGAC 
     2161 TGGGCTGGCG ATGAGCGAAA TGTAGTGCTT ACGTTGTCCC GCATTTGGTA CAGCGCAGTA 
     2221 ACCGGCAAAA TCGCGCCGAA GGATGTCGCT GCCGACTGGG CAATGGAGCG CCTGCCGGCC 
     2281 CAGTATCAGC CCGTCATACT TGAAGCTAGA CAGGCTTATC TTGGACAAGA AGAAGATCGC 
     2341 TTGGCCTCGC GCGCAGATCA GTTGGAAGAA TTTGTCCACT ACGTGAAAGG CGAGATCACC 
     2401 AAGGTAGTCG GCAAATAACC CTCGAGCCAC CCATGACCAA AATCCCTTAA CGTGAGTTAC 
     2461 GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 
     2521 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 
     2581 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 
     2641 CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 
     2701 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 
     2761 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 
     2821 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 
     2881 CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 
     2941 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 
     3001 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 
     3061 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 
     3121 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATG
//